RESUMO
BACKGROUND: Topical exogenous lipase has been approved for cosmetic use and has been used to mobilize fat from adipocytes. The objective of this study was to determine the effects of exogenous lipase in the subcutaneous adipose tissue. METHODS: Three different concentrations of exogenous lipase 1× (2 Units per ml), 5× (10 units per ml), and 10× (20 units per ml) were applied in a porcine model. Normal saline (NS) solution (as negative control) and phosphatidylcholine (as positive control) were also injected. Skin and subcutaneous tissue biopsies, up to the fascia, were obtained from each injection site on the 3rd day after injection. The number of cells per 20× field was counted as an indirect measurement of the size of the adipocytes. RESULTS: For 1× lipase, the number of cells per field was 47.80 (±7.63) versus 27.26 (±4.93), and 34.66 (±6.84) for NS, and phosphatidylcholine, respectively. For 5× lipase, the count was 36.06 (±4.74) versus 24.13 (±5.18), and 33.2 (±9.34). For 10× lipase, it was 40.06 (±4.35) versus 29.26 (±2.34) and 32.66 (±6.30) (p < .05 for all groups). CONCLUSIONS: A higher number of cells per field were observed in the lipase samples, inferring a decreased volume of adipocytes. No inflammation and/or loss of cell architecture were evidenced in the exogenous lipase groups.
Assuntos
Tecido Adiposo , Lipase , Suínos , Animais , Lipase/farmacologia , Tecido Adiposo/patologia , Gordura Subcutânea , Fosfatidilcolinas/farmacologia , Modelos AnimaisRESUMO
OBJETIVO: Evaluar los resultados estéticos y funcionales, así como el grado de satisfacción, posteriores a la reconstrucción de defectos de la extremidad inferior en niños con colgajo de dorsal ancho preservador del músculo como una alternativa para estos casos. MÉTODO: Se incluyeron pacientes menores de 18 años con defectos en la extremidad inferior tratados en un periodo de 3 años. Se realizó colgajo dorsal ancho preservador de músculo en todos los pacientes. Se valoraron la apariencia estética, la fuerza muscular del área donadora y la función en el área receptora a los 3 meses de realizado el colgajo. RESULTADOS: Se realizaron 13 colgajos de dorsal ancho preservadores de músculo. La apariencia estética de las áreas donadora y receptora fue satisfactoria en la mayoría de los casos. La mayoría de los pacientes presentaron una fuerza muscular normal (M5) en el sitio donador y pudieron deambular. CONCLUSIONES: El colgajo de dorsal ancho preservador de músculo tiene un grado de satisfacción muy elevado, excelente simetría y preservación de la función muscular del sitio donador. Es una técnica ideal en defectos tisulares importantes de la extremidad inferior en la población pediátrica. OBJECTIVE: To evaluate the aesthetic and functional outcomes as well as the degree of postoperative satisfaction of the lower limb's defects reconstruction with a muscle-sparing latissimus dorsi flap in children in order to propose this kind of reconstruction as a secure alternative. METHOD: In this study were included patients under 18 years with lower limb's defects treated over a period of 3 years. A muscle-sparing latissimus dorsi flaps was performed in all patients. After three months the flap was performed, it was evaluated the donor's area muscular strength, recipient area function and aesthetic appearance. RESULTS: They were made 13 muscle-sparing latissimus dorsi flaps. In most cases, the donor and recipient area has satisfactory aesthetic appearance, most of the patients had a normal muscular strength (M5) in donors area and were able to walk. CONCLUSIONS: The reconstruction using a muscle-sparing latissimus dorsi flap has a very high level of satisfaction, excellent symmetry, and preservation of donor area muscle function. It is an ideal technique for major tissue defects of lower limb in children.
Assuntos
Retalhos Cirúrgicos , Adolescente , Criança , Humanos , Estudos RetrospectivosRESUMO
Remarkable advances in burn care have been made over recent decades, and it is recognized that the organized efforts of burn teams are required to continue enhancing survival rates and quality of life for patients. Patients with major burns are unique, representing one of the most severe models of trauma, and therefore necessitate treatment in the best specialized facilities available for that endeavor. Burn centers have developed to meet these intricate needs but can only function most productively and efficiently through well-organized, multifaceted, patient-centered teams in the areas of both clinical care and research.