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1.
Molecules ; 28(15)2023 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-37570879

RESUMO

The present investigation aimed to develop inclusion complexes (ICs) from Psidium gaudichaudianum (GAU) essential oil (EO) and its major compound ß-caryophyllene (ß-CAR), and to evaluate their herbicidal (against Lolium multiflorum and Bidens pilosa) and cytogenotoxic (on Lactuca sativa) activities. The ICs were obtained using 2-hydroxypropyl-ß-cyclodextrin (HPßCD) and they were prepared to avoid or reduce the volatility and degradation of GAU EO and ß-CAR. The ICs obtained showed a complexation efficiency of 91.5 and 83.9% for GAU EO and ß-CAR, respectively. The IC of GAU EO at a concentration of 3000 µg mL-1 displayed a significant effect against weed species B. pilosa and L. multiflorum. However, the ß-CAR IC at a concentration of 3000 µg mL-1 was effective only on L. multiflorum. In addition, the cytogenotoxic activity evaluation revealed that there was a reduction in the mitotic index and an increase in chromosomal abnormalities. The produced ICs were able to protect the EO and ß-CAR from volatility and degradation, with a high thermal stability, and they also enabled the solubilization of the EO and ß-CAR in water without the addition of an organic solvent. Therefore, it is possible to indicate the obtained products as potential candidates for commercial exploration since the ICs allow the complexed EO to exhibit a more stable chemical constitution than pure EO under storage conditions.


Assuntos
Herbicidas , Óleos Voláteis , Psidium , 2-Hidroxipropil-beta-Ciclodextrina/química , Herbicidas/farmacologia , Herbicidas/análise , Óleos Voláteis/química , Folhas de Planta/química , Psidium/química , Solubilidade
2.
Sci Rep ; 13(1): 3930, 2023 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-36894661

RESUMO

Terpenoids are essential for plant growth, development, defense, and adaptation mechanisms. Psidium cattleyanum (Myrtaceae) is a fleshy fruit tree species endemics from Atlantic Forest, known for its pleasant fragrance and sweet taste, attributed to terpenoids in its leaves and fruits. In this study, we conducted genome-wide identification, evolutionary and expression analyses of the terpene synthase gene (TPS) family in P. cattleyanum red guava (var. cattleyanum), and yellow guava (var. lucidum Hort.) morphotypes. We identified 32 full-length TPS in red guava (RedTPS) and 30 in yellow guava (YlwTPS). We showed different expression patterns of TPS paralogous in the two morphotypes, suggesting the existence of distinct gene regulation mechanisms and their influence on the final essential oil content in both morphotypes. Moreover, the oil profile of red guava was dominated by 1,8-cineole and linalool and yellow guava was enriched in α-pinene, coincident in proportion to TPS-b1 genes, which encode enzymes that produce cyclic monoterpenes, suggesting a lineage-specific subfamily expansion of this family. Finally, we identified amino acid residues near the catalytic center and functional areas under positive selection. Our findings provide valuable insights into the terpene biosynthesis in a Neotropical Myrtaceae species and their potential involvement in adaptation mechanisms.


Assuntos
Myrtaceae , Psidium , Frutas/química , Myrtaceae/metabolismo , Psidium/genética , Psidium/química , Terpenos/metabolismo , Genoma de Planta
3.
Sci Rep ; 13(1): 1385, 2023 01 25.
Artigo em Inglês | MEDLINE | ID: mdl-36697447

RESUMO

Diploid and polyploid species derived from the euploid series x = 11 occur in the genus Psidium, as well as intraspecific cytotypes. Euploidy in the genus can alter the gene copy number, resulting in several "omics" variations. We revisited the euploidy, reported genomic (nuclear 2C value, GC%, and copy number of secondary metabolism genes) and epigenomic (5-mC%) differences in Psidium, and related them to essential oil yield and composition. Mean 2C values ranged from 0.90 pg (P. guajava) to 7.40 pg (P. gaudichaudianum). 2C value is intraspecifically varied in P. cattleyanum and P. gaudichaudianum, evidencing cytotypes that can be formed from euploid (non-reduced) and/or aneuploid reproductive cells. GC% ranged from 34.33% (P. guineense) to 48.95% (P. myrtoides), and intraspecific variations occurred even for species without 2C value intraspecific variation. Essential oil yield increased in relation to 2C value and to GC%. We showed that P. guajava (diploid) possesses two and P. guineense (tetraploid) four copies of the one specific TPS gene, as well as eight and sixteen copies respectively of the conserved regions that occur in eight TPS genes. We provide a wide "omics'' characterization of Psidium and show the outcome of the genome and epigenome variation in secondary metabolism.


Assuntos
Óleos Voláteis , Psidium , Epigenômica , Genômica , Poliploidia
4.
Nat Prod Res ; 37(12): 2013-2017, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35979669

RESUMO

Inclusion complexes (ICs) of 2-hydroxypropyl-ß-cyclodextrin with the essential oil (EO) from Seculo XXI cultivar of Psidium guajava were prepared using kneading (KN) and freeze-drying (FD) methods. The resulting ICs clusters have a nanometric size, with a diameter of approximately 80 and 40 nm for KN and FD, respectively. Complexation efficiency was 80.3% and 50.8% for KN and FD methods, respectively. The larvicidal activity of the EO in DMSO on A. aegypti had LC50 and LC90 values of 51.49 and 64.51 µg mL-1, respectively. For the KN method, the toxicity corresponded to 77.54 and 107.29 µg mL-1 for LC50 and LC90, respectively. FD method demonstrated toxicity at concentrations above 600 µg mL-1. Thus, ICs enable the use of EO in breeding sites for A. aegypti, thus being potential products to be commercially exploited.


Assuntos
Aedes , Inseticidas , Óleos Voláteis , Psidium , Animais , Óleos Voláteis/farmacologia , 2-Hidroxipropil-beta-Ciclodextrina , Larva , Inseticidas/farmacologia
5.
Food Res Int ; 160: 111676, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36076387

RESUMO

Essential oils (EOs) have been considered potential green additives for active food packaging. However, sub-lethal concentrations of EOs may lead to bacterial resistance, which is a concern. In this sense, the effects of 1% (GEO1) and 10% (GEO10) of garlic EO in cellulose acetate-based films regarding homologous resistance in Listeria innocua were investigated after incubation at 37 °C/24 h and 7 °C/10 d. The films were also characterized and tested on sliced mozzarella cheese as interfold packaging for 8-days storage at 7 °C. The EO did not alter the mechanical properties of the films nor their thermal degradation profile. However, GEO10 was less permeable to water vapor than GEO1. When tested against L. innocua, the incubation at 7 °C enhanced the films' antimicrobial effect: log reductions of 4.3 and 5.7 were obtained for GEO1 and GEO10, respectively. Moreover, 86.3% of L. innocua cells were injured at sub-lethal level when exposed to GEO10. Despite this, no occurrence of homologous resistance was found. When the active films were tested on cheese against the natural microbiota, they resulted in slices of mozzarella with fewer contaminants, however the reduction was not significant. Nevertheless, we considered this an important finding to the food industry since this work suggested that GEO is a safe active compound from the point of view of homologous resistance to be used against Listeria.


Assuntos
Alho , Listeria , Óleos Voláteis , Celulose/análogos & derivados , Microbiologia de Alimentos , Óleos Voláteis/farmacologia
6.
Sci Rep ; 11(1): 24408, 2021 12 23.
Artigo em Inglês | MEDLINE | ID: mdl-34949763

RESUMO

Some forest trees have been polyploidized to improve their traits and to supply new germplasms for breeding programs. As trees have a long juvenile stage, the early characterization of the chromosome set doubling effects is crucial for previous selection. Thus, we aimed to characterize the chemical variability of essential oils from diploid and autotetraploid germplasms (autotetraploid A and B) of Eucalyptus benthamii, as well as to evaluate their larvicidal and allelopathic effects. Autotetraploid A showed a higher essential oil yield than diploid and autotetraploid B, which did not differ quantitatively. Aromadendrene, viridiflorol and α-pinene were the major compounds in the diploid essential oil. In contrast, compounds were present in autotetraploids, such as 1,8-cineole, limonene, α-terpineol, and α-terpinyl-acetate. Essential oils from the diploid at 50-200 ppm were twice as larvicidal than those from autotetraploids against Aedes aegypti larvae. Considering the phytotoxicity bioassays using Lactuca sativa, essential oils from both ploidy levels affected root growth. Moreover, the essential oils inhibited shoot growth at all concentrations tested (187.5; 375; 750; 1500; and 3000 ppm). Autotetraploid A and B had the same effect on shoot growth as glyphosate. The essential oils had no cytogenotoxic effect on root meristematic cells of L. sativa, whereas phytotoxic potential was identified mainly in shoot growth. This work demonstrated a dramatic change in secondary metabolism (terpene composition) related to an increase in the ploidy level in Eucalyptus germplasms. In addition, we report the novelty of the chemical composition of essential oils among germplasms and their potential use as larvicidal and post-emergence weed control agents.


Assuntos
Óleo de Eucalipto/química , Óleo de Eucalipto/farmacologia , Eucalyptus/química , Eucalyptus/genética , Herbicidas , Inseticidas , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Tetraploidia , Aedes/efeitos dos fármacos , Alelopatia/efeitos dos fármacos , Animais , Bioensaio , Relação Dose-Resposta a Droga , Larva/efeitos dos fármacos , Lactuca/efeitos dos fármacos , Lactuca/crescimento & desenvolvimento , Melhoramento Vegetal , Brotos de Planta/efeitos dos fármacos , Brotos de Planta/crescimento & desenvolvimento
7.
Sci Rep ; 10(1): 12213, 2020 07 22.
Artigo em Inglês | MEDLINE | ID: mdl-32699377

RESUMO

The intensive application of agrochemicals in crops has negatively impacted the environment and other organisms. The use of naturally occurring compounds may be an alternative to mitigate these effects. Plants are secondary metabolite reservoirs and may present allelopathic activity, which is potentially interesting to be used in bioherbicide formulations. In this context, the present work aimed to evaluate the phytotoxic and cytotoxic effects of essential oils extracted from leaves of Sparattanthelium botocudorum and Sparattanthelium tupiniquinorum in bioassays with the plant models Lactuca sativa L. and Sorghum bicolor L. Moench. The essential oils were applied at concentrations of 3,000, 1,500, 750, 375 and 187.5 ppm. Chemical characterization of the oils was performed, and their impact on the percentage of germinated seeds, initial development of L. sativa and S. bicolor seedlings, and changes in the mitotic cycle of meristematic cells from L. sativa roots was evaluated. The major compound of the essential oils was germacrene D, followed by bicyclogermacrene, ß-elemene and germacrene A. The phytotoxicity assay showed that the essential oils of both species reduced the root and shoot growth in L. sativa and decreased the germination and shoot growth in S. bicolor. Inhibition was dependent on the tested oil concentration. In the cytotoxicity assay, a decrease in mitotic index and chromosomal and nuclear alterations were observed, which resulted from aneugenic and clastogenic action.


Assuntos
Hernandiaceae/metabolismo , Óleos Voláteis/química , Plântula/efeitos dos fármacos , Compostos Orgânicos Voláteis/farmacologia , Cromatografia Gasosa , Germinação/efeitos dos fármacos , Hernandiaceae/química , Lactuca/efeitos dos fármacos , Lactuca/crescimento & desenvolvimento , Mitose/efeitos dos fármacos , Óleos Voláteis/análise , Óleos Voláteis/farmacologia , Folhas de Planta/química , Folhas de Planta/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Brotos de Planta/efeitos dos fármacos , Brotos de Planta/crescimento & desenvolvimento , Sementes/crescimento & desenvolvimento , Sesquiterpenos de Germacrano/farmacologia , Sorghum/efeitos dos fármacos , Sorghum/crescimento & desenvolvimento , Compostos Orgânicos Voláteis/análise , Compostos Orgânicos Voláteis/química
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