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2.
Sci Rep ; 9(1): 10356, 2019 07 25.
Artigo em Inglês | MEDLINE | ID: mdl-31346187

RESUMO

Increasingly large proportions of tropical forests are anthropogenically disturbed. Where natural regeneration is possible at all, it requires the input of plant seeds through seed dispersal from the forest matrix. Zoochorous seed dispersal - the major seed dispersal mode for woody plants in tropical forests - is particularly important for natural regeneration. In this study, covering a period of more than 20 years, we show that small New World primates, the tamarins Saguinus mystax and Leontocebus nigrifrons, increase their use of an anthropogenically disturbed area over time and disperse seeds from primary forest tree species into this area. Through monitoring the fate of seeds and through parentage analyses of seedlings of the legume Parkia panurensis from the disturbed area and candidate parents from the primary forest matrix, we show that tamarin seed dispersal is effective and contributes to the natural regeneration of the disturbed area.


Assuntos
Callitrichinae , Florestas , Dispersão de Sementes , Animais , Biodiversidade , Conservação dos Recursos Naturais , Fabaceae/genética , Fabaceae/crescimento & desenvolvimento , Comportamento Alimentar , Humanos , Periodicidade , Estações do Ano , Plântula/genética , Plântula/crescimento & desenvolvimento , Clima Tropical
3.
Appl Plant Sci ; 3(4)2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25909042

RESUMO

PREMISE OF THE STUDY: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing. METHODS AND RESULTS: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. CONCLUSIONS: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.

4.
Am J Bot ; 98(5): e127-9, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21613179

RESUMO

PREMISE OF THE STUDY: We present a set of 12 polymorphic nuclear microsatellite loci, identified for the first time within the herbaceous plant species Cnidium dubium (Schkuhr) Thell. using next-generation sequencing. METHODS AND RESULTS: To characterize these loci, 40 plants were analyzed genetically. The number of alleles ranged from 2 to 23, with an average of 8.3 alleles per locus. Mean observed and expected heterozygosities ranged from 0.25 to 0.95 and 0.30 to 0.94, respectively. CONCLUSIONS: All 12 nuclear microsatellite loci showed scorable and polymorphic fragments after polymerase chain reaction. The new marker set can be used for future studies of genetic diversity and differentiation as well as estimation of gene flow and spatial genetic structures.


Assuntos
Cnidium/genética , Primers do DNA/genética , DNA de Plantas/genética , Repetições de Microssatélites , Núcleo Celular/genética , Alemanha , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polimorfismo Genético
5.
Am J Bot ; 97(5): e34-6, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-21622435

RESUMO

PREMISE OF THE STUDY: We present here a set of nine polymorphic nuclear microsatellite loci, identified for the first time within the neotropical legume tree species Parkia panurensis Benth. ex H. C. Hopkins, which is widespread in western and central Amazonia. METHODS AND RESULTS: To characterize these loci, 33 Parkia panurensis adult trees were analyzed. The number of alleles ranged from eight to 32, with an average of 14.4 alleles per locus. Mean expected heterozygosity ranged from 0.74 to 0.955. CONCLUSIONS: All nine loci could also be verified in six other Parkia species and polymorphic fragments amplified. The new marker set can be used for future studies of genetic diversity and differentiation, as well as estimation of gene flow and parentage analyses in various Parkia species.

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