RESUMO
Birds of the partially inbred G-B1 chicken line can be classified as either high or low responders to Con A, on the basis of the amount of 3H-thymidine incorporated by Con A-containing cultures of their peripheral blood leukocytes. The pattern of inheritance of the high and low responder traits suggests that the variation in response is due to genetic polymorphism at a single autosomal locus. However, the allele responsible for the low responder trait of the G-B1 line is not identical to the allele of the previously described Mr1 locus carried by the inbred low responder CC line, since (CC x G-B1 low responder)F1 birds are uniformly high responders to Con A. The responses to Con A of mixtures of CC and G-B1 low responder cells are not significantly higher than the responses of either component of the mixture alone. Thus, the gene products responsible for the complementation observed in F1 hybrids cannot complement extracellularly, and are not readily transferable from one cell to another.
Assuntos
Galinhas/genética , Concanavalina A/farmacologia , Ativação Linfocitária , Linfócitos/imunologia , Animais , Cruzamentos Genéticos , Teste de Complementação Genética , Linhagem , Polimorfismo GenéticoRESUMO
In a population of (CB X CC)F1 X WB hybrids, a chicken was found with a presumably recombinant haplotype, BR1, whose antigenic products detectable by hemagglutination contained determinants derived from both parental haplotypes, i.e. B1 (from CB) and B2 (from CC). This recombinant bird and its progeny from different crosses were tested by skin grafting, graft-vs.-host (GVH) and mixed lymphocyte reactions (MLR). The results define two regions of the B system, the B-F and the B-G. The B-F region determined serologically defined antigens, histocompatibility antigens, and controlled the GVH and MLR reactions, while the B-G region was responsible only for synthesis of serologically detectable antigens.
Assuntos
Epitopos , Antígenos de Histocompatibilidade , Recombinação Genética , Animais , Galinhas , Feminino , Genótipo , Reação Enxerto-Hospedeiro , Teste de Cultura Mista de Linfócitos , Masculino , Transplante de Pele , Transplante HomólogoRESUMO
A microtechnique (10 mul culture volume) for in vitro lymphocyte cultures is described, which, compared with current miniaturized techniques, permits a four to 20-fold reduction of both medium volume and cell numbers. Furthermore, two alternative procedures for harvesting and washing radioisotope labelled cells are described. The first, making use of a conventional harvesting device, collects cells on glass fibre filters in a two to three-fold shorter time than that needed for the current semi-automatic collectors. The second takes advantage of the possibility of washing and fixing the cells in their culture wells and allows processing of more cultures with fewer manual operations. Various technical aspects of the micro-culture system are described and discussed with special reference to automation problems.