Assuntos
Hepatopatias/sangue , alfa 1-Antitripsina/análise , Adolescente , alfa-Globulinas/análise , Criança , Pré-Escolar , Doença Crônica , Humanos , LactenteAssuntos
Hepatite B/imunologia , Hepatite Crônica/imunologia , Adolescente , Formação de Anticorpos , Criança , Pré-Escolar , Feminino , Humanos , Imunidade Celular , MasculinoAssuntos
Cárie Dentária/etiologia , Hemofilia A/imunologia , Doenças Periodontais/etiologia , Adolescente , Adulto , Cárie Dentária/imunologia , Feminino , Humanos , Imunoglobulina A/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Masculino , Higiene Bucal , Doenças Periodontais/imunologiaRESUMO
The occurrence of circulating immune complexes was tested in 43 children with different forms of glomerulonephritis (GN). The presence of immune complexes was demonstrated in sera of 6 out of 10 patients with GN of hepatitis B etiology, in 6 out of 11 patients with GN of bacterial etiology, and in 3 out of 10 patients with GN of unknown etiology. Immune complexes were not found in sera of 3 patients with Schönlein-Henoch disease, in 6 patients with lipoid nephrosis, and in 3 patients with GN and hepatitis B as a coexisting infection. In a patient with membrane-capillary GN which appeared after infection with hepatitis B virus we demonstrated immune complexes of M.W. 4.0 X 10(6) and 7.5 X 10(5).
Assuntos
Complexo Antígeno-Anticorpo/isolamento & purificação , Glomerulonefrite/imunologia , Adolescente , Infecções Bacterianas/complicações , Criança , Pré-Escolar , Feminino , Glomerulonefrite/etiologia , Hepatite B/complicações , Humanos , Vasculite por IgA/imunologia , Lactente , Masculino , Peso Molecular , Nefrose Lipoide/imunologiaRESUMO
Polyethylene glycol (PEG)-laser nephelometer assay for the detection of circulating immune complexes (i.c.) has been developed. The method proved to be useful in the detection of i.c. in sera of rabbits with chronic serum sickness. I.c. were detected in 28 animal experimental sera and in samples with preformed immune complexes, but not in sera of 10 healthy rabbits and 5 rabbits prior to immunization. Laser nephelometer assay is a rapid, simple, quantitative screening test suited for clinical use as a first step in i.c. detection.
Assuntos
Complexo Antígeno-Anticorpo/análise , Imunoensaio/métodos , Doença do Soro/imunologia , Animais , Precipitação Química , Imunodifusão , Lasers , Nefelometria e Turbidimetria/métodos , Polietilenoglicóis , Coelhos , Soroalbumina Bovina/imunologia , Fatores de TempoRESUMO
Antibody to hepatitis B core antigen in the sera of 10 patients with acute viral hepatitis B, 20 patients with chronic hepatitis B, and 30 carriers of chronic hepatitis B surface antigen was assayed by the indirect immunofluorescence test. The activity of antibody to hepatitis B core antigen in the sera of these three groups of patients was identified as belonging to immunoglobulin classes G, M, A, and D. Antibody to hepatitis B core antigen was predominantly of IgG and IgM classes in all three groups of patients.