Different ratios of raw material triggered composting maturity associated with bacterial community co-occurrence patterns.

Exploring the ecological function of potential core bacteria for high-efficiency composting can provide a fundamental understanding of the role of composting bacterial communities. Mushroom residue and kitchen garbage at different ratios (N1: 1/1, N2: 1/2) of dry weight were tested to investigate the key ecological role of the core bacteria responsible for producing mature compost. N1 had a peak temperature of 75.0 °C which was higher than N2 (68.3 °C). Other key composting parameters (carbon to nitrogen ratio (C/N) and germination index (GI)) also indicated that N1 achieved higher compost maturity. Rice seedlings experiments also further validated this conclusion. Putative key bacterial taxa (Thermobifida, Luteimonasd, Bacillus, etc.) were positively associated with the GI, indicating a substantial contribution to composting maturity. Co-occurrence network analysis revealed the ecological function of potentially beneficial core bacteria promoted cooperation among the bacterial community. The putative core bacteria in N1 may affect composting efficiency. Our findings reveal the mechanism of potential core bacteria throughout the compost maturity phases.

Determining the Effects of Compost Substitution on Carbon Sequestration, Greenhouse Gas Emission, Soil Microbial Community Changes, and Crop Yield in a Wheat Field.

Compost produced by straw and livestock and poultry manure under the action of micro-organisms is one of the main forms of organic alternative fertilizers at present. The present study explored the effects of compost substitution on soil greenhouse gas emissions, soil microbial community changes, and wheat yield to determine the best substitution ratio for reducing greenhouse gas emissions and soil microbial community changes and increasing wheat yield. Using the single-factor randomized block trial design, four treatments were employed, the characteristics of greenhouse gas emission, yield and yield components, and the changes of soil microbial community under different compost substitution ratio in the whole wheat growing season were determined by static box-gas chromatography. During the wheat season, both CO2 and N2O emissions were reduced, whereas CH4 emission was increased. That all treatments reduced the Global Warming Potential (GWP) and Greenhouse gas emission intensity (GHGI) in wheat season compared with T0. Compost substitution can alleviate the global warming potential to some extent. Under the condition of compost substitution, the wheat yield under T2 and T3 increased significantly compared with that under the control; however, the spike number and 1000-grain weight did not differ significantly among the treatments. When compost replacement was 30%, the yield was the highest. Under different ratios of compost substitution, the microbial communities mainly comprised Proteobacteria, Actinobacteria, Firmicutes, Patescibacteria, Chloroflexi, Acidobacteria, Bacteroidetes, Gemmatimonadetes, and Verrucomicrobia. The soil microbial community structure differed mainly due to the difference in the compost substitution ratio and was clustered into different groups. In conclusion, to achieve high wheat yield and low greenhouse gas emissions, compost replacement of 30% is the most reasonable means for soil improvement and fertilization.