On-Chip Optical Anodic Stripping with Closed Bipolar Cells and Cathodic Electrochemiluminescence Reporting.

The aim of this work was to develop a simple, accessible, and point-of-use sensor to measure heavy metal ions in water in low-resource areas that cannot accommodate expensive or technical solutions. This report describes a new bipolar electrochemical sensor platform that reimagines conventional anodic stripping voltammetry in a wireless bipolar format with an optical electrochemiluminescent readout that can be quantified with any simple optical sensor like that found on most modern cell phone cameras. We call this technique as optical anodic stripping. Using a new nonlithographic fabrication process, devices could be produced rapidly and simply at <$1/sensor. The sensing scheme was developed, characterized, and optimized using electrochemical and optical methods. Quantitation of Pb2+ in both lab and natural water samples was rapid (2-3 min), accurate, precise, and highly linear in the 25-1000 ppb range and was shown to be sufficiently selective in the presence of other common heavy metal ions such as Cu2+, Cd2+, and Zn2+.

Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization.

The correct assembly, migration, and segregation of the mRNAs of the germ plasm during the first cell divisions are intimately connected to the cytoskeleton and cytokinesis.RhoA is a key regulator of germ plasm localization during the first two cell division cycles in zebrafish embryos. Pharmacological inhibition of RhoA and his effector ROCK affected the correct assembly of microtubules in the cleavage furrow with the concomitant abnormal localization of germ plasm mRNAs. The inhibition of RhoA/ROCK pathway caused a significant decrease in the germ cell population later in development.