RESUMO
We tested whether the probability of detecting avian haemosporidia (Plasmodium and Haemoproteus) using molecular techniques differs among blood, liver, heart, and pectoral muscle tissues. We used a paired design, sampling the 4 tissue types in 55 individuals of a wild South American suboscine antbird, the white-shouldered fire-eye (Pyriglena leucoptera). We also identified parasites to cytochrome b lineage. Detection probability was significantly lower in blood compared to the other 3 tissue types combined. Eight of 22 infections were not detected in blood samples; 4-7 infections were not detected in the other individual tissues. The same parasite lineage was recovered from different tissues.
Assuntos
Haemosporida/isolamento & purificação , Malária Aviária/parasitologia , Passeriformes/parasitologia , Animais , Brasil , Citocromos b/genética , DNA Mitocondrial/química , DNA Mitocondrial/isolamento & purificação , DNA de Protozoário/química , DNA de Protozoário/isolamento & purificação , Coração/parasitologia , Fígado/parasitologia , Malária Aviária/sangue , Passeriformes/sangue , Músculos Peitorais/parasitologia , Plasmodium/isolamento & purificaçãoRESUMO
We described in this paper the first synthesis to the (+/-) cis (6-ethyl-tetrahydropyran-2-yl) formic acid (1) using the very efficient Prins cyclization reaction as strategy to construction of its tetrahydropyran skeleton. This new compound presented a significant antinociceptive property by the tail-flick model.