Sandwich-type electrochemical aptasensor based on hemin-graphite oxide as a signal label and rGO/MWCNTs/chitosan/carbon quantum dot modified electrode for sensitive detection of Acinetobacter baumannii bacteria.

Acinetobacter baumannii (A. baumannii) is a pathogenic bacterium that causes severe infections and its rapid and reliable diagnosis is essential for effective control and treatment. In this study, we present an electrochemical aptasensor based on a signal amplification strategy for the detection of A. baumannii, the high specificity and affinity of the aptamer for the target make it favorable for signal amplification. This allows for a highly sensitive and selective detection of the target. The aptasensor is based on a carbon screen-printed electrode (CSPE) that has been modified with a nanocomposite consisting of multi-walled carbon nanotubes (MWCNTs), reduced graphene oxide (rGO), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS. Additionally, the self-assembled aptamers were immobilized on hemin-graphite oxide (H-GO) as a signal probe. The composition of the nanocomposite (rGO-MWCNT/CS/CQD) provides high conductivity and stability, facilitating the efficient capture of A. baumannii onto the surface of the aptasensor. Also, aptamer immobilized on Hemin-graphite oxide (H-GO/Aptamer) was utilized as an electrochemical signal reporter probe by H reduction. This approach improved the detection sensitivity and the aptamer surface density for detecting A. baumannii. Furthermore, under optimized experimental conditions, the aptasensor was demonstrated to be capable of detecting A. baumannii with a linear range of (10 - 1 × 107 Colony-forming unit (CFU)/mL) and a limit of detection (LOD) of 1 CFU/mL (σ = 3). One of the key features of this aptasensor is its ability to distinguish between live and dead bacteria cells, which is very important and critical for clinical applications. In addition, we have successfully detected A. baumannii bacteria in healthy human serum and skim milk powder samples provided using the prepared electrochemical aptasensor. The functional groups present in the synthetic CQD, rGO-MWCNT, and chitosan facilitate biomolecule immobilization and enhance stability and activity. The fast electron-transfer kinetics and high conductivity of these materials contribute to improved sensitivity and selectivity. Furthermore, The H-GO/Aptamer composite's large surface area increases the number of immobilized secondary aptamers and enables a more stable structure. This large surface area also facilitates more H loading, leading to signal amplification.

One Pot Synthesis and Biological Activity Studies of New Spirooxindoles.

This article reports one-pot synthesis of ten novel spirooxindoles using 5-methyl-2-thiohydantoin, isatin derivatives, and malononitrile in good to high yields (65-90 %). The structures of the synthesized compounds were deduced by 1H-NMR, 13C NMR, FT-IR, and Mass spectral data. The antibacterial activity of the compounds was evaluated against two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) based on the Kirby-Bauer method. According to the obtained data, the synthesized compounds show more activity against Gram-positive bacteria than Gram-negative bacteria. Also, the antioxidant activity of these compounds was measured using the DPPH radical scavenging test method, which showed good to excellent activity (59.65-94.03 %). Among them, the chlorinated derivatives (4 f-j) exhibited more antioxidant activity (84.85-94.03 %) than the other compounds (4 a-e) (56.65-74.4 %) and even ascorbic acid as a standard antioxidant compound (82.3 %).

Implications of ALS-Associated Mutations on Biochemical and Biophysical Features of hSOD1 and Aggregation Formation.

One of the recognized motor neuron degenerative disorders is amyotrophic lateral sclerosis (ALS). By now, several mutations have been reported and linked to ALS patients, some of which are induced by mutations in the human superoxide dismutase (hSOD1) gene. The ALS-provoking mutations are located throughout the structure of hSOD1 and promote the propensity to aggregate. Despite numerous investigations, the underlying mechanism related to the toxicity of mutant hSOD1 through the gain of a toxic function is still vague. We surveyed two mutant forms of hSOD1 by removing and adding cysteine at positions 146 and 72, respectively, to investigate the biochemical characterization and amyloid formation. Our findings predicted the harmful and destabilizing impact of two SOD1 mutants using multiple programs. The specific activity of the wild-type form was about 1.42- and 1.92-fold higher than that of C146R and G72C mutants, respectively. Comparative structural studies using CD spectropolarimetry, and intrinsic and ANS fluorescence showed alterations in secondary structure content, exposure of hydrophobic patches, and structural compactness of WT-hSOD1 vs. mutants. We demonstrated that two mutants were able to promote amyloid-like aggregates under amyloid induction circumstances (50-mM Tris-HCl pH 7.4, 0.2-M KSCN, 50-mM DTT, 37 °C, 190 rpm). Monitoring aggregates were done using an enhancement in thioflavin T fluorescence and alterations in Congo red absorption. The mutants accelerated fibrillation with subsequently greater fluorescence amplitude and a shorter lag time compared to WT-SOD1. These findings support the aggregation of ALS-associated SOD1 mutants as an integral part of ALS pathology.

Preparation of Some New Pyrazolo[1,5-a]pyrimidines and Evaluation of Their Antioxidant, Antibacterial (MIC and ZOI) Activities, and Cytotoxic Effect on MCF-7 Cell Lines.

This study aims to synthesize some novel pyrazolo[1,5-a]pyrimidine derivatives, and investigate their biological activities. These compounds exhibited good to high antioxidant activities [2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capabilities]. Among them, Ethyl 5-(2-ethoxy-2-oxoethyl)-7-hydroxy-2-methylpyrazolo[1,5-a]pyrimidine-3-carboxylate (3h) showed the highest antioxidant activity [Half-maximal Inhibitory Concentration (IC50 )=15.34 µM] compared to ascorbic acid (IC50 =13.53 µM) as a standard compound. Their antibacterial activities were investigated against two Gram-positive bacteria (Bacillus subtilis, and Staphylococcus aureus) and two Gram-negative bacteria (Pseudomonas aeruginosa, and Escherichia coli). The results showed that Ethyl 7-hydroxy-5-phenylpyrazolo[1,5-a]pyrimidine-3-carboxylate (3i) has the best antibacterial activity against Gram-positive B. subtilis [Zone of Inhibition (ZOI)=23.0±1.4 mm, Minimum Inhibitory Concentration (MIC)=312 µM]. Also, the cytotoxicity of these compounds was assessed against breast cancer cell lines [human breast adenocarcinoma (MCF-7)], which 7-Hydroxy-2-methyl-5-phenylpyrazolo[1,5-a]pyrimidine-3-carbonitrile (3f) displayed the most cytotoxicity (IC50 =55.97 µg/mL), in contrast with Lapatinib (IC50 =79.38 µg/mL) as a known drug.

Development of a label-free impedimetric aptasensor for the detection of Acinetobacter baumannii bacteria.

Acinetobacter baumannii (A. baumannii) is responsible for various nosocomial infections, which is known as a clinically crucial opportunistic pathogen. Therefore, rapid detection of this pathogen is critical to prevent the spread of infection and appropriate treatment. Biological detection probes, such as aptamers and synthetic receptors can be used as diagnostic layers to detect bacteria. In this work, an electrochemical aptasensor was developed for the ultrasensitive detection of A. baumannii by electrochemical impedance spectroscopy (EIS). The aptamer was immobilized on the surface of a CSPE modified with the nanocomposite Fe3O4@SiO2@Glyoxal (Gly) for selective and label-free detection of A. baumannii. The charge transfers resistance (Rct) between redox couple [Fe(CN)63-/4-] and the surface of aptasensor in the Nyquist plot of EIS study was used as electroanalytical signal for detection and determination of A. baumannii. The obtained results showed that the constructed aptasensor could specifically detect A. baumannii in the concentration range from 1.0 × 103-1.0 × 108 Colony-forming unit (CFU)/mL and with a detection limit of 150 CFU/mL (S/N = 3). In addition to its sensitivity, the biosensor exhibits high selectivity over some other pathogens. Therefore, a simple, inexpensive, rapid, label-free, selective, and sensitive electrochemical aptasensor was developed to detect A. baumannii.

A signal-off aptasensor for the determination of Acinetobacter baumannii by using methylene blue as an electrochemical probe.

An electrochemical aptasensor has been developed to detect Acinetobacter baumannii (A. baumannii). The proposed system was developed by modifying carbon screen-printed electrodes (CSPEs) with a synthesized MWCNT@Fe3O4@SiO2-Cl nanocomposite and then binding A. baumannii-specific aptamer using covalent immobilization on the modified electrode surface and the interaction of methylene blue (MB) with Apt as an electrochemical redox indicator. As a result of the incubation of the A. baumannii bacteria as a target on the proposed aptasensor, a cathodic peak current density (Jpc) of MB decreased due to the formation of the Apt-A. baumannii complex and MB being released from the immobilized Apt on the surface of the modified electrode. In addition to increasing the electron transfer kinetics, the nanocomposite provides a relatively stable matrix to improve the loading Apt sequence. The suggested aptasensor was demonstrated to be capable of detecting A. baumannii with a linear range of 10.0-1.0 × 107 colony-forming unit (CFU) mL-1 and a detection limit of 1 CFU mL-1 (S/N = 3) using differential pulse voltammetry (DPV) studies at a working potential of ~0.29 V and a scan rate of 100 mV s-1. The outcomes revealed that the aptasensor exhibited high A. baumannii detection sensitivity, stability, reproducibility, and specificity.

Toward decentralized wastewater treatment: A flow-through module using microtubular gas diffusion electrodes for micropollutants removal.

Electro-Fenton (EF) represents an eco-friendly and cost-effective advanced oxidation process that can remove highly persistent and hazardous pharmaceuticals, e.g., contrast media agents, from water bodies. However, up to date, EF modules incorporate a planar carbonaceous gas diffusion electrode (GDE) cathode containing fluorinated compounds as polymeric binders. Here, we introduce a novel flow-through module that deploys freestanding carbon microtubes (CMT) as microtubular GDEs, omitting any risks of secondary pollution by highly-persistent fluorinated compounds (e.g., Nafion). The flow-through module was characterized for electrochemical hydrogen peroxide (H2O2) generation and micropollutant removal via EF. H2O2 electro-generation experiments illustrated high production rates (1.1 ± 0.1-2.7 ± 0.1 mg cm-2 h-1) at an applied cathodic potential of - 0.6 V vs. SHE, depending on the porosity of CMTs. Diatrizoate (DTZ), as the model pollutant, with a high initial concentration of 100 mg L-1 was successfully oxidized (95-100 %), reaching mineralization (TOC-total organic carbon removal) efficiencies up to 69 %. Additionally, Electro-adsorption experiments demonstrated the capability of positively charged CMTs to remove negatively charged DTZ with a capacity of 11 mg g-1 from a 10 mg L-1 DTZ solution. These results reveal the potential of the as-designed module to serve as an oxidation unit coupled with other separation techniques, e.g., electro-adsorption or membrane processes.

EfectroH2O: Development and evaluation of a novel treatment technology for high-brine industrial wastewater.

Textile production is one of the main sources of freshwater consumption by industries worldwide. In addition, according to the world bank, 20 % of the wastewater generated globally is caused by textile wet-processing. Textile wet-processing includes the processes in textile production where garments are dyed or given the final functions like water-repellency. Several thousand chemicals were used in this process, some of which are highly toxic. Discharging untreated or insufficiently treated wastewater in water bodies results in high pollution levels, severely impacting the environment and human health. Especially in textile-producing countries like India, environmental pollution and water consumption from textile wet-processing have severe impacts. Next to the high volume of chemicals used in textile production, the high salt concentration in textile wastewater also poses a challenge and is critical for freshwater systems. Moreover, textile wastewater is one of the most difficult to treat wastewater. Currently, used treatment technologies do not meet the requirements to treat textile wastewater. Therefore, the further development of efficient treatment technologies for textile wastewater is critically important. Hence, in the interdisciplinary project, effect-based monitoring demonstrates the efficiency of electrically-driven water treatment processes to remove salts and micropollutants from process water (EfectroH2O), a low-energy Zero Liquid Discharge (ZLD) textile wastewater treatment technology is being developed consisting of a combination of capacitive deionization (CDI) and advanced oxidation processes (AOP). In addition to treatment technology development, methods for evaluating the efficiency of treatment technologies also need to be improved. Currently, mainly physicochemical parameters such as pH, biochemical oxygen demand (BOD) and chemical oxygen demand (COD) are tested worldwide to check water quality. However, these methods are insufficient to make a statement about the toxic potential of such complex mixtures as textile wastewater. Therefore, also next to chemical analyses, effect-based methods (EBM) are used to verify the treated wastewater.

Sandwich-Type Electrochemical Aptasensor for Highly Sensitive and Selective Detection of Pseudomonas Aeruginosa Bacteria Using a Dual Signal Amplification Strategy.

An electrochemical aptasensor developed to realize the detection of Pseudomonas aeruginosa (P. aeruginosa) bacteria based on a signal amplification strategy. The carbon screen-printed electrode (CSPE) surface was modified by MIL-101(Cr)/Multi-walled carbon nanotubes (MWCNT), which significantly increased the effective surface area of the electrode, thus resulting in further F23 aptamer immobilization at the surface of the modified electrode. As a result, the P. aeruginosa can be efficiently captured onto the surface of the aptasensor. Moreover, aptamer immobilized on the two-dimensional graphitic carbon nitride complex with silver nanoparticles (AgNPs/c-g-C3N4/Apt) was used as an electrochemical signal label, connected to P. aeruginosa bacteria at the modified electrode. This strategy increased the aptamer surface density and the sensitivity for detecting P. aeruginosa. Also, the resultant material was thoroughly characterized using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis techniques. A highly sensitive voltammetric aptasensor for P. aeruginosa detection was obtained via this strategy at the limit of detection of 1 Colony-forming unit (CFU)/mL (σ = 3). Therefore, this proposed strategy with dual signal amplification can be a promising platform for simple, practical, reliable, and sensitive method for P. aeruginosa.

Highly efficient, bioactive, and bifunctional sorbent p-n-p visible light heterogeneous photocatalyst utilizing ultra-fine ZnS nanoparticles embedded in a polymeric nanocomposite.

This study reports the successful synthesis of a ZnS@GO@Pani polymeric nanocomposite (NC) via chemical polymerization. The product was used for simultaneous photocatalytic degradation-adsorption of malachite green (MG), a carcinogenic and widely used dye. The physicochemical properties of the prepared NC were characterized by various techniques. Morphological and XRD results confirmed the fine size of ZnS nanoparticles (NPs) with an approximate mean size of 5 nm, uniformly distributed within the polymeric matrix. For comparative purposes, photocatalytic dye degradation-adsorption of this nanohybrid was explored both in the dark and under natural light. It was observed that 0.1 g of the ternary NC in MG aqueous solution (20 ppm) leads to dye adsorption within 15 minutes with an efficiency of 70% under dark conditions. Also, MG removal efficiency of up to 90% was achieved in 15 minutes under natural light owing to integrated photocatalytic degradation-adsorption mechanisms. Adsorption isotherm studies were performed considering Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich (D-R) models. The results showed that the Freundlich isotherm with R 2 = 0.988 is well consistent with the experimental data. Integrated photocatalytic degradation-adsorption kinetics were modeled with pseudo-first-order (PFO) and pseudo-second-order (PSO) models where PSO with R 2 = 0.999 best fitted the data, implying the predominant role of chemical adsorption in the dye removal process. Antibacterial tests revealed superior antibacterial activity of the prepared ZnS@GO@Pani NC against both Gram-negative and Gram-positive bacteria, demonstrating the remarkable synergistic effect of ZnS NPs embedded in the GO@Pani matrix. Accordingly, the prepared NC could be regarded as a promising candidate for wastewater treatment applications. The leaching and regeneration studies also confirmed that the prepared NC is a non-toxic dye removal agent with good reusability.

Freestanding Nitrogen-Doped Carbons with Hierarchical Porosity for Environmental Applications: A Green Templating Route with Bio-Based Precursors.

Powdery hierarchical porous carbons serve as cost-effective, functional materials in various fields, namely energy storage, heterogeneous catalysis, electrochemistry, and water/wastewater treatment. Such powdered activated carbons (PAC) limit new module designs and require further preparation steps, for example, adding polymeric binders, to be shaped into a standalone geometry. Polymeric binders, however, can block PACs' catalytic and active sites and, more importantly, pose the risk of secondary pollution for environmental purposes, especially in the context of clean water supply. This study introduces a novel synthesis method for fabricating freestanding nitrogen-doped carbons with hierarchical porosity using chitosan and sucrose as green precursors. Chitosan supplies nitrogen and acts as a backbone, giving a freestanding geometry to the final product, and sucrose is a carbon-rich precursor. The proposed method employs ice- and hard-templating for macropores and mesopores and combines carbonization and activation steps with no required activating agent. Final freestanding carbons function as adsorbents for removing persistent pollutants, as binder-free electrodes with high specific surface area and capacitive current, and as tubular gas diffusion electrodes for oxygen reduction reactions. These freestanding carbons enable new module designs and can be scaled-up by numbering-up, serving as bio-based functional materials for a wide range of applications involving porous heteroatom-doped carbons.

Antimicrobial activity, environmental sensitivity, mechanism of action, and food application of αs165-181 peptide.

αs165-181 is a peptide derived from αs2-casein of ovine milk. Herein, we report the antimicrobial activity and mechanism, and food application of the peptide. αs165-181 showed antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Listeria monocytogenes, Bacillus cereus, and Salmonella enterica serovar Enteritidis in a dose-dependent manner. The minimum inhibitory concentration of the peptide was 3.9 mg/ml for E. coli and 7.8 mg/ml for the other bacteria. The peptide did not show antimicrobial activity against Lactobacillus plantarum up to 3.9 mg/ml concentration. The minimum bactericidal concentration of αs165-181 peptide was 7.8 mg/ml for E. coli, S. aureus, L. monocytogenes, and B. cereus. The peptide was sensitive to monovalent and divalent cations, pH, and high temperatures. Transmission electron microscopy, cytoplasmic ß-galactosidase leakage, and DNA electrophoresis analyses showed that αs165-181 peptide affects bacteria by damaging cell membrane and binding to the genomic DNA. When αs165-181 peptide was applied to minced beef or UHT cream, the antimicrobial activity (7.8 mg/g) was almost the same as or even better than nisin (0.5 mg/g). This study helps understand the antimicrobial mode of action of αs165-181 peptide and develop strategies for application in food products.

Wet-Spun PEDOT/CNT Composite Hollow Fibers as Flexible Electrodes for H2O2 Production.

The electrochemical synthesis of hydrogen peroxide (H2O2) using the oxygen reduction reaction (ORR) requires highly catalytic active, selective, and stable electrode materials to realize a green and efficient process. The present publication shows for the first time the application of a facile one-step bottom-up wet-spinning approach for the continuous fabrication of stable and flexible tubular poly(3,4-ethylene dioxythiophene) (PEDOT : PSS) and PEDOT : PSS/carbon nanotube (CNT) hollow fibers. Additionally, electrochemical experiments reveal the catalytic activity of acid-treated PEDOT : PSS and its composites in the ORR forming hydrogen peroxide for the first time. Under optimized conditions, the composite electrodes with 40 wt % CNT loading could achieve a high production rate of 0.01 mg/min/cm2 and a current efficiency of up to 54 %. In addition to the high production rate, the composite hollow fiber has proven its long-term stability with 95 % current retention after 20 h of hydrogen peroxide production.

Preparation of New Spiropyrazole, Pyrazole and Hydantoin Derivatives and Investigation of Their Antioxidant and Antibacterial Activities.

In this study, the synthesis of new spiropyrazoles, pyrazole and hydantoin heterocycles is reported by three component reactions of parabanic acids, hydrazine derivatives, and phenacyl bromides in the presence of triphenylphosphine as a nucleophile and triethylamine as a base in good to high yields (69-91 %). Evaluation of the synthesized compounds revealed a good to excellent antioxidant activities (37.6-96.2 %) using DPPH inhibitory potency. Among these compounds, hydantoin derivatives displayed higher antioxidant activities (93.7-96.2 %) comparing with spiropyrazoles and pyrazoles. The obtained results showed that Cl and Br substituents on the phenyl ring increased antioxidant activities of the related heterocycles. The antibacterial activities of the synthesized compounds were examined against two Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and two Gram-positive (Staphylococcus aureus and Bacillus subtilis) bacteria. Among the synthesized heterocycles, 2-[1,3-dimethyl-2,5-dioxo-4-(2-oxo-2-phenylethyl)imidazolidin-4-yl]hydrazine-1-carbothioamide exhibited the excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.

Effects of parenteral administration of tylosin and ivermectin on abomasal emptying rate in healthy suckling lambs by use of nuclear scintigraphy.

Abomasal hypomotility is one of the important causes of neonatal mortality in small ruminants. Various pharmaceutical agents have been studied to address this problem in large ruminants. The aim of this study was to determine the effect of parenteral administration of tylosin and ivermectin on abomasal emptying rate in neonatal suckling lambs. Abomasal emptying rate was assessed using nuclear scintigraphic method in 10 healthy female Iranian fat tailed Ghezel lambs. Each lamb was tested three times, once as a control (1 ml of saline 0.9%, IM) and twice after the injection of tylosin (17.6 mg/kg, IM) and ivermectin (200 µg/kg, SC) in a crossover study. Based on radiopharmaceutical counts, remnant activity in abomasums at 90 min were 48.3 ± 3.5, 45.6 ± 7.5 and 41.6 ± 2.9% in control, tylosin and ivermectin groups, respectively. Administration of tylosin (p = 0.049) and ivermectin (p = 0.045) to lambs, significantly caused faster abomasal emptying rate compared to control. Evaluating the ROIs revealed that the half emptying time (T1/2) in control, tylosin and ivermectin groups were 67.1 ± 8.6, 62.6 ± 14.2 and 54.3 ± 9.9 min, respectively. These difference between all groups, statistically were significant (p = 0.026). However, the clinical efficacy of abomasal emptying rate facilitating by tylosin or ivermectin administration in lambs remains to be determined.

Biodegradable macro-porous CMC-polyaniline hydrogel: synthesis, characterization and study of microbial elimination and sorption capacity of dyes from waste water.

Certainly water pollution control will play a key role in environmental health. Therefore, researchers are attempting to reduce the ecological effects of water pollution by creating restrictions in the use of chemicals or water reuse. Among all the available techniques, adsorption method attracted much attention due to the higher efficiency, cost-effectiveness and simplicity. So, in this work, novel biodegradable hydrogel based on carboxymethyl cellulose (CMC) and polyaniline (PANI) was introduced to remove toxic dyes from wastewater. The synthesis process was accomplished in two steps: free radical polymerization of acrylic acid (AA) on the CMC (0.25 g) in the presence of ammonium per sulfate (APS) as radical initiator (0.2 g) and N,N-methylene-bis-acrylamide (MBA) as crosslinker (0.1 g) at 70 °C, and after 30 min, growing PANI chains on the synthesized CMC-PAA hydrogel by radical polymerization of aniline (1.0 mL) in acidic condition (20 mL of hydrochloric acid 1.0 M) to form macro-porous conductive hydrogel (CMC-PAA-PANI). The resulted hydrogel showed high antibacterial activity and excellent biodegradability by natural soil microorganisms with decomposition to 91.7 %. Also, the final hydrogel exhibited reasonable conductivity and pH sensitivity properties.

Antibacterial and Cytotoxic Effects of Cyclodextrin-Triazole-Titanium Based Nanocomposite

Abstract In this paper, the antibacterial activity of triazole functionalized cyclodextrin (CD.Click) and cyclodextrin-triazole-titanium based nanocomposite (CD.COM) was evaluated. The results indicated that CD.Click and CD.COM perform a wide range of antibacterial activity against both gram positive (Staphylococcus aureus and Bacillus subtilis) and gram negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The cytotoxic effect of CD.COM was investigated in vitro on cancerous cell lines (cervical cancer, breast carcinoma and sarcoma osteogenic) and fibroblast cells by MTT assay. The cell viability evaluation confirmed that the growth of cancerous cells is inhibited in a dose and time dependent way without any significant effect on the normal fibroblast cells.

Synthesis of bioactive polyaniline-b-polyacrylic acid copolymer nanofibrils as an effective antibacterial and anticancer agent in cancer therapy, especially for HT29 treatment.

In this work, a new highly water-soluble copolymer of polyacrylic acid with polyaniline is introduced. Acrylic acid was polymerized via the Reversible Addition Fragmentation Chain Transfer method (RAFT) in the presence of an initiator and the obtained polyacrylic acid was copolymerized with aniline at room temperature. As the main achievements of this work, the resulting block copolymer with nanosized structure revealed favorable solubility in polar solvents, as well as excellent antibacterial and anticancer activities. Therefore, it is an appropriate candidate for medical applications such as wound healing and cancer therapy, especially in HT29 treatment.