[Conformational disturbances of serum albumin binding sites in schizophrenia].

The properties of serum albumin binding sites were studied using quenching of fluorescence of the molecular probe CAPIDAN (N-carboxyphenylimide of dimethylaminonaphthalic acid) with the nitrate anion. The samples of serum were obtained from 24 patients with paranoid schizophrenia and 24 healthy volunteers. In the absence of quencher the specific probe fluorescence was 1,4 times higher in patients than in volunteers. Fluorescence quenching constant for the probe bound to albumin was (M+/-m) 2,48+/-0,17 l/mol in patients versus 4,65+/-0,37 l/mol (p<0,01) in volunteers (p<0,01). The fluorescence fraction assessable to quenching was significantly (p<0,01) lower in schizophrenic patients as compared to controls (0,60+/-0,03 and 0,76+/-0,03) respectively). Thus, it is shown that in patients with schizophrenia the conformational state of albumin binding sites is significantly changed as compared to controls that can lead to the changes in the protein-ligand interaction and, thus, contribute to the pathogenesis of the disease and patient's response to treatment.

[Effects of haloperidol therapy on dynamics of serum albumin binding capacity in patients with schizophrenia]. / Vliianie terapii galoperidolom na dinamiku pokazatelei sviazyvaiushchei sposobnosti al'bumina syvorotki krovi u bol'nykh shizofreniei.

Time course of blood serum albumin binding capacity was studied by the fluorescent method in schizophrenics, including those treated with haloperidol. Effective and total albumin concentrations were measured and binding reserve estimated, which was equal to ratio of effective to total albumin concentrations multiplied by 100%. Effective albumin concentrations and albumin binding reserve were found markedly reduced in the patients vs. healthy donors. Drastic changes of albumin binding reserve from one examination to another and a trend to increase of effective concentration of albumin were characteristic of patients administered haloperidol. In the patients administered no haloperidol albumin binding reserve was as a rule unchanged and effective albumin concentration had a trend to reduction.