Biocompatibility, osseointegration, antibacterial and mechanical properties of nanocrystalline Ti-Cu alloy as a new orthopedic material.

The demands for high-performance biomaterials are driving the development of new metallic alloys with improved mechanical and biological responses. In this study, a nanocrystalline Ti-Cu intermetallic alloy was prepared by a powder metallurgy route, and its application as an orthopedic material was evaluated by the microstructural, mechanical, corrosion, antibacterial, cytotoxicity and osseointegration examinations. Microstructural characterization revealed the formation of TiCu and Ti2Cu3 as major phases with 23 nm grain size in the structure of the alloy. The synthesized alloy exhibited ultra-high hardness of 10 GPa, acceptable toughness of 8.14 MPam1/2, a ∼98 % anti-bacterial rate against S. aureus and E. coli, excellent cell viability to MG-63 osteosarcoma cells, and high osteoblast formation rate, which indicate a great potential of this alloy for biomedical application.

Antibacterial activity of ultra-small copper oxide (II) nanoparticles synthesized by mechanochemical processing against S. aureus and E. coli.

In this paper, ultra-small CuO nanoparticles (NPs) were synthesized through a mechanochemical method using two different Cu-containing precursors (i.e. CuSO4·5H2O and CuCl2·2H2O), and their structure and antibacterial activity were studied. From the microstructural studies, it was observed that CuO NPs have a spherical morphology and a narrow size distribution with 7 and 14 nm median particle sizes for CuCl2·2H2O and CuSO4.5H2O precursors, respectively. The CuCl2·2H2O derived nanoparticles showed more antibacterial activity than CuSO4.5H2O derived nanoparticles. The minimum inhibitory concentration (MIC) of the synthesized nanoparticles (derived from both precursors) against E. coli and S.aureus were 3.75 and 2.50 mg/ml, respectively, which are higher than those reported in the literature for CuO NPs synthesized by other methods. This difference may be originated from ultra-small size of the synthesized nanoparticles, high bandgap energy and Fe inclusion entering from milling media and their effect on oxidative stress-mediated cytotoxicity of CuO NPs. The higher MIC value reported in this work indicates that the synthesized NPs not only show good antibacterial activity, but also they yield lower cytotoxicity, which extends their applications in the biomedical field.