Effect of Decantation Time on Viability and Apoptosis in Adipocytes After Liposuction.

BACKGROUND: The effect of decantation time on viability and apoptosis in adipocytes has not been described. The objective of the study was to describe viability and apoptosis in adipocytes up to 2 h after harvesting. METHODS: Twenty patients who underwent esthetic liposuction from the abdomen were included. The lipoaspirate was obtained from the infra-umbilical area with the tumescent technique. Liposuction was performed with a 60-ml syringe and a 3-ml cannula. Lipoaspirates were centrifuged at 50 g for 5 min at 0, 60 and 120 min after harvesting. One gram of fat was digested with 0.1% type 1 collagenase and incubated at 37 degrees for 30 min. Adipocytes were counted on 10 random microscopic fields. Apoptosis was determined by TUNEL assay. A fluorescence microscope was used to visualize the staining nuclei and cells. RESULTS: Regarding viability, immediately after harvesting, 57.6 ± 18.9% of the cells were viable, whereas 60 min after liposuction the viability decreased to 51.62 ± 8.8% and 120 min after liposuction the percentage of viable cells was 46.8 ± 16.9%. The percentage of apoptotic cells at time 0 was 38.2 ± 8.0%, whereas it was 51.24 ± 8.1% at 60 min and 62.9 ± 16.1% at 120 min after collection. CONCLUSIONS: Apoptosis and mortality of adipocytes after liposuction increase directly proportional to the time of decantation. Lipoinjection should be performed as soon as possible after harvesting. NO LEVEL ASSIGNED: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Variables diferenciadoras del nivel de gravedad de la depresión mediante el Sistema Comprehensivo Rorschach / Differentiated variables of the seriousness level of the depression by means of the Rorschach Comprehensive System

RESUMEN Se desarrolló una investigación descriptiva transversal, con un enfoque cuantitativo, en la que la muestra fue seleccionada de manera intencional pura, no probabilística, según criterios establecidos por los autores del estudio. Quedó constituida por las variables del Sistema Comprehensivo Rorschach pertenecientes a 120 protocolos de sujetos con trastornos depresivos, de ellos 82 presentaron depresión moderada, y 38 depresión severa. Se aplicaron los siguientes métodos empíricos: entrevista, observación, Autoescala de Zung y Conde, y el Psicodiagnóstico de Rorschach. Los estadígrafos que se utilizaron fueron el Test de Kolmogorov- Smirnov, el Test de Comparación de Proporciones y la prueba de Mann-Whitney. Como principales resultados se obtuvo que las variables del Sistema Comprehensivo Rorschach, que establecieron diferencias estadísticas altamente significativas entre los grupos de sujetos fueron: Lambda alta, Proporción afectiva baja, total de respuestas, las respuestas globales, Suma Ponderada de respuestas con el determinante color cromático y la Experiencia accesible.

ABSTRACT A transverse descriptive investigation was developed, with a quantitative approach, in that the sample was selected in a pure intentional way, not probabilistic, according to criteria established by the authors of the study. It was constituted for the variables of the Rorschach Comprehensive System belonging to 120 protocols of individuals with depressive disorders, of them 82 presented moderate depression, and 38 severe depression. The following empirical methods were applied: interview, observation, Auto-scale of Zung and Conde, and Psycho-diagnosis of Rorschach. The statisticians used were the Test of Kolmogorov - Smirnov, the Test of Comparison of Proportions and the Mann-Whitney test. As main results, it was obtained that the variables of the Rorschach Comprehensive System, which established highly significant statistical differences between the groups of individuals were: tall Lambda, low affective proportion, whole of answers, the global answers, exaggerated sum of answers with the determinant chromatic color and the accessible experience.

Reservorios de leptospirosis en Camagüey: dos resultados y una misma base de datos / Leptospirosis reservoirs in Camagüey: two results and a same database

El objetivo del trabajo fue establecer la fiabilidad de las variables número de muestras y proporción de reactores positivos por especie para el estudio de los animales domésticos como reservorios de leptospirosis en Camagüey. Se hizo una base de datos con los reactores a Leptospira a partir de los sueros sanguíneos de 2 659 bovinos, 2 617 equinos, 814 porcinos, 75 ovinos-caprinos y 69 caninos de la provincia. Los resultados, atendiendo al número de muestras, dieron a bovinos (43 por ciento) y equinos (41 por ciento) como reactores hegemónicos; porcinos (13 por ciento), caninos (1 por ciento) y ovino-caprinos (1 por ciento) mostraron un comportamiento muy inferior. En función de la proporción de reactores por especie, bovinos, porcinos, caninos y caballares prevalecieron homogéneamente (P<0,05) a diferencia de ovino-caprino. Los caballos destinados a la transportación urbana constituyeron un riesgo potencial para el ser humano

The objective of the work was to establish the reliability of the variable number of samples and proportion of positive reactors by species for the study of the domestic animals as leptospirosis reservoirs in Camagüey. A database was made with the reactors to Leptospira from the sanguine serums of 2 659 bovine, 2 617 equine, 814 swinish, 75 ovines-caprines and 69 canines of the province. According to the number of samples, the results gave to bovine (43 percent) and equine (41 percen) as hegemonic reactors; swinish (13 percen), canine (1 percent) and ovine-caprines (1 percen) showed a very inferior behavior. In function of the proportion of reactors by species, bovine, swinish, canine and horses prevailed homogeneously (P<0,05) contrary to ovine-caprine. Horses dedicated to the urban transportation constituted a potential risk for the human being.

Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Based on Surveillance Programs in Freshwater Trout Farms of Mexico.

Diagnostic testing was performed between 2000 and 2012 to determine the distribution of infectious pancreatic necrosis virus (IPNV) in the main states of the Mexican Republic with freshwater Rainbow Trout Oncorhynchus mykiss (Walbaum) farms. This virus was positively identified from Rainbow Trout farms in seven of the eight states assessed. Due to nonnormal data distribution, a logistic regression model was applied for statistical analysis, the results of which indicated that virus prevalence was variable between states, with moderate but significant differences. Regarding the time periods evaluated, IPNV prevalence was higher during the first years of the study. The susceptible, infected, removed model was used to examine this phenomenon, which indicated that the decreased prevalence during the latter years of the study could be associated with a real elimination of the infection. The information of the cases analyzed also suggests a relationship with the irregularity in the submission of samples to the laboratory and emphasizes other factors that have contributed to the transmission of IPNV throughout the country. Received November 10, 2014; accepted December 5, 2015.

Obtención de extractos salubres de tres variedades de frijol común (Phaseolus vulgaris) con actividad mitogénica sobre linfocitos humanos: determinación de su confiabilidad por el uso clínico / Mitogenic activity of 3 solubre extract of common beans (Phaseolus vulgaris): their reliability for clinical use

Se describe un método económico, sencillo y reproducible para obtener un extracto salubre de semillas de tres variedades de frijol común (Phaseolus vulgaris), con actividad mitogénica para linfócitos humanos, cuya potencia es dependiente de la dosis empleada y comparable a la de un producto comercial de importación y alto costo. Se realizaron 193 análisis citogenéticos durante un período de tres años. Con ello se confirmó la utilidad y confiabilidad del producto para uso clínico. La actividad mitogénica de los extractos de frijol, obtenidos mediante el método aqui descrito, es estable durante un año por lo menos, almacenado a -20-C, y cuando menos por cinco meses a 4-C