Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
Mais filtros

Base de dados
Tipo de documento
Intervalo de ano de publicação
1.
J Manipulative Physiol Ther ; 38(2): 119-29, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25487299

RESUMO

OBJECTIVE: The purpose of this study was to investigate oxidative-stress parameters in individuals with chronic neck or back pain after 5 weeks of treatment with high-velocity, low-amplitude (HVLA) spinal manipulation. METHODS: Twenty-three individuals aged 38.2 ± 11.7 years with nonspecific chronic neck or back pain verified by the Brazilian Portuguese version of the Chronic Pain Grade, with a sedentary lifestyle, no comorbidities, and not in adjuvant therapy, underwent treatment with HVLA chiropractic manipulation twice weekly for 5 weeks. Therapeutic procedures were carried out by an experienced chiropractor. Blood samples were assessed before and after treatment to determine the activities of the antioxidant enzymes superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx), and the levels of nitric oxide metabolites and lipid hydroperoxides. These blood markers were analyzed by paired Student t test. Differences were considered statistically significant, when P was <.05. RESULTS: There was no change in catalase but an increase in SOD (0.35 ± 0.03 U SOD per milligram of protein vs 0.44 ± 0.04 U SOD per milligram of protein; P < .05) and GPx (7.91 ± 0.61 nmol/min per milligram of protein vs 14.07 ± 1.07 nmol/min per milligram of protein; P < .001) activities after the treatment. The nitric oxide metabolites and the lipid hydroperoxides did not change after treatment. CONCLUSION: High-velocity, low-amplitude spinal manipulation twice weekly for 5 weeks increases the SOD and GPx activities. Previous studies have shown a relationship between pain and oxidative and nitrosative parameters; thus, it is possible that changes in these enzymes might be related to the analgesic effect of HVLA spinal manipulation.


Assuntos
Dor Lombar/reabilitação , Manipulação Quiroprática/métodos , Manipulação da Coluna/métodos , Cervicalgia/reabilitação , Estresse Oxidativo/fisiologia , Adulto , Biomarcadores/sangue , Brasil , Catalase/metabolismo , Dor Crônica/reabilitação , Estudos de Coortes , Feminino , Humanos , Dor Lombar/sangue , Dor Lombar/diagnóstico , Masculino , Pessoa de Meia-Idade , Cervicalgia/sangue , Cervicalgia/diagnóstico , Óxido Nítrico/sangue , Índice de Gravidade de Doença , Superóxido Dismutase/sangue , Resultado do Tratamento
2.
Neurosci Lett ; 569: 163-8, 2014 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-24704379

RESUMO

Since N-acetylcysteine (NAC) is a donor of cysteine, we studied the relationship between NAC and concentration of oxidized and reduced glutathione (GSH/GSSG ratio), and glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities in the lumbosacral spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve that received NAC (150mg/kg/day, i.p.) or 0.9% saline solution for 3 or 10 days. Hydrogen peroxide (H2O2) and nitric-oxide (NO) metabolites were also measured. Von Frey hair and hot-plate tests showed hyperalgesia at day 1 in CCI rats. Hyperalgesia persisted at all other times in saline-treated CCI rats, but returned to pre-injury values in NAC-treated CCI rats after 3 postoperative days. GST activity and the GSH/GSSG ratio increased in saline-treated CCI rats, while the NAC treatment increased GST and GPx activities at day 10, with no significant change in the GSH/GSSG ratio. NAC treatment did not affect H2O2 levels, but it reduced NO metabolites in CCI rats 3 days after the surgery. Thus, the anti-hyperalgesic effect of NAC appears not to involve its action as a cysteine precursor for GSH synthesis, but involves a decrease in NO.


Assuntos
Acetilcisteína/farmacologia , Analgésicos/farmacologia , Glutationa Peroxidase/metabolismo , Glutationa Transferase/metabolismo , Glutationa/metabolismo , Neuralgia/metabolismo , Medula Espinal/efeitos dos fármacos , Animais , Constrição Patológica , Temperatura Alta , Peróxido de Hidrogênio/metabolismo , Hiperalgesia/fisiopatologia , Região Lombossacral , Masculino , Neuralgia/fisiopatologia , Óxido Nítrico/metabolismo , Estimulação Física , Ratos Wistar , Nervo Isquiático/lesões , Medula Espinal/metabolismo , Tato
3.
Neurochem Res ; 37(9): 1952-8, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22674084

RESUMO

Although reactive oxygen species (ROS) are involved in neuropathic pain, the direct relationship between these species and chronic constriction of sciatic nerve (CCI) has not been studied in spinal cord. Thus, this study induced CCI in rats and these animals were sacrificed 3 and 10 days after the surgical procedure to determine the superoxide dismutase (SOD) and catalase activities, as well as ascorbic acid, hydrogen peroxide (H(2)O(2)) and lipid hydroperoxide levels in lumbosacral spinal cord. Von Frey Hair and hot plate tests were performed to assess the degree of mechanical and thermal hyperalgesia at days 0, 3 and 10. The results showed that CCI significantly induced mechanical and thermal hyperalgesia at days 3 and 10. Parallel there was increase in spinal cord lipid hydroperoxide at days 3 and 10 in rats submitted to CCI. In Sham rats a significant increase in this parameter occurred at day 10. H(2)O(2) decreased at day 10 only in CCI group. SOD activity was decreased in Sham and CCI groups at day 3, while catalase activity was increased in CCI rats at days 3 and 10. Ascorbic acid levels were reduced only in CCI rats at day 3. Although the role of such changes is unclear, many were not specific to neuropathic pain and the differences could be related to different degrees of central sensitization in Sham and CCI rats.


Assuntos
Neuropatia Ciática/metabolismo , Medula Espinal/metabolismo , Animais , Ácido Ascórbico/metabolismo , Comportamento Animal , Catalase/metabolismo , Doença Crônica , Constrição Patológica , Temperatura Alta , Peróxido de Hidrogênio/metabolismo , Hiperalgesia/metabolismo , Peróxidos Lipídicos/metabolismo , Masculino , Proteínas do Tecido Nervoso/metabolismo , Oxirredução , Estimulação Física , Ratos , Ratos Wistar , Neuropatia Ciática/psicologia , Medula Espinal/patologia , Superóxido Dismutase/metabolismo
4.
J Manipulative Physiol Ther ; 35(4): 295-300, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22632589

RESUMO

OBJECTIVE: This study investigates the analgesic effect of high-velocity, low-amplitude (HVLA) manipulation and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) in erythrocytes of men with neck pain. METHODS: Twenty-two men with neck pain of mechanical origin who were aged 20 to 50 years, were nonsmokers, had a sedentary lifestyle, had no comorbidities, and were not in adjuvant therapy underwent 6 sessions of HVLA chiropractic manipulation 3 times a week for 2 weeks. Patients were treated by the same chiropractor and under the same conditions. Blood samples were collected before the beginning of the treatment and at the end of the third and last session. Erythrocytes were separated from blood and then processed to determine SOD and GPx activities. The quadruple visual scale and the Neck Disability Index were used to demonstrate the analgesic effect of treatment. The results were analyzed by repeated-measures analysis of variance followed by Bonferroni posttest. Differences were considered significant when P was less than .05. RESULTS: Despite the tendency to reduction in SOD and increase in GPx activities, there was no significant change after the treatment. CONCLUSION: High-velocity, low-amplitude treatment for 6 sessions in men with neck pain did not affect systemic SOD and GPx activities. Despite the absence of significant changes, this study is important because it is the first to investigate the activities of SOD and GPx in patients with neck pain treated with HVLA spinal manipulation.


Assuntos
Eritrócitos/enzimologia , Glutationa Peroxidase/metabolismo , Manipulação Quiroprática/métodos , Manipulação da Coluna/métodos , Cervicalgia/enzimologia , Cervicalgia/terapia , Superóxido Dismutase/metabolismo , Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Cervicalgia/sangue , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA