[Use of monoclonal antibodies for detecting Aujeszky's disease virus in culture media by an immunoenzyme method]. / Ispol'zovanie monoklonal'nykh antitel dlia opredeleniia immunofermentnym metodom virusa bolezni aueski v kul'tural'noi srede.

Three modifications of quantitative enzyme immunoassay (EIA) for assessment of Aujeszky's disease virus (ADV) in culture medium are compared, in which monoclonal antibodies (MAB) to ADV GH glycoprotein were used: nonconcurrent two-site "sandwich" EIA on the basis of two different MABs, indirect concurrent EIA, and direct concurrent EIA. MABs fit best of all for use in every of EIA modifications were selected. Conditions of the assays were optimized. Concurrent EIAs were 10-20 times less sensitive than sandwich EIA. The developed sandwich EIA permitted ADV assay in preparations of both live and formaldehyde-inactivated virus.

[The isolation and characteristics of monoclonal antibodies to the glycoprotein GII of Aujeszky's disease virus and their use for the epitopic mapping of GII]. / Poluchenie i kharakteristika monoklonal'nykh antitel k glikoproteinu GII virusa bolezni Aueski i ikh ispol'zovanie dlia épitopnogo kartirovaniia GII.

A panel of 24 different monoclonal antibodies (MAB) to Aujeszky's disease virus (ADV) proteins was prepared. Seven MABs were directed to ADV glycoprotein GII (6 MABs to GIIc chain and 1 MAB to GIIb chain). GII epitope mapping with the resultant MABs was carried out. Four ADV GII epitopes were detected: epitopes A, B, and C located on GIIc chain and epitope D located on GIIb chain. Epitope B overlaps epitopes A and C which do not overlap each other, epitope D does not overlap other epitopes. Epitopes A, B, and C are located in a restricted area of GIIc chain which seems to be one of GII immunodominant regions. The detected epitopes are mainly conformational, for they are sensitive to denaturation. Of all MABs obtained more than 30% were directed to ADV GII. This indicates that GII is one of the most important ADV antigens. All the seven MABs to GII interacted with all tested ADV strains (K. Arsky, VGNKI, BUK, and NIA-4), this being in line with the data on a highly conservative nature of ADV GII.

[Isolation and characterization of Pseudomonas aeruginosa bacteriophage phikF77 mutants]. / Vydelenie i kharakteristika mutatsionnykh proizvodnykh bakteriofaga phikF77 Pseudomonas aeruginosa.

It was found that phage phi kF77 is resistant to all known Pseudomonas aeruginosa restriction systems. Three types of mutants (dc-) which were unable to grow on different restrictive strains were isolated. All of them belong to one complementation group. Some of these mutations affected also the number of nicks in phage phi kF77 DNA and increased phage resistance to temperature treatment. It may be supposed that genes responsible for antirestriction mechanisms and introduction of nicks into DNA are connected in definite way.

[Incompatibility groups of epsilon-caprolactam biodegradation plasmids from bacteria of Pseudomonas genus]. / Gruppy nesovmestimosti plazmid biodegradatsii epsilon-kaprolaktama bakterii roda Pseudomonas.

Incompatibility of epsilon-caprolactam biodegradation plasmids pBS262, pBS263, pBS264, pBS265, pBS266, pBS267, pBS268, pBS270, pBS276, pBS269 with the tester plasmids of P-1, P-2, P-7, P-9 incompatibility groups in the system of strains of P. putida line BSA, as well as the character of plasmid interaction with the number of P. aeruginosa and P. putida bacteriophages have been studied. The majority of the studied plasmids belongs to IncP-7, IncP-9 or simultaneously to IncP-7 and IncP-9 incompatibility groups. The ability to restrict the growth of some bacteriophages of P. aeruginosa and P. putida has been demonstrated for some plasmids.

[Bacteriophages of Pseudomonas putida containing single-stranded canonical DNA breaks]. / Bakteriofagi Pseudomonas putida, soderzhashchie odnonitevye kanonicheskie razryvy v DNK.

It was shown that bacteriophage tf as well as bacteriophages phi p4/40, phi p25/42, phi p23/40 and phi p6/40, which are specific to different P. putida strains, contain the single strand breaks in their DNA. The breaks are localized in one strand of DNA molecules and are repairable with T4 DNA ligase. Bacteriophage tf has no detectable DNA homology with phi p4/40, phi p25/42, phi p23/40 and phi p6/40 bacteriophages. All the phages studied have no relation with other known Pseudomonas phages. Bacteriophages phi p4/40 and phi p25/42 share the extensive DNA homology.