RESUMO
It is shown that the transport of oxygen through the surface of the isolated perfused rat liver is an energy-dependent process that requires the energy of ATP hydrolysis.
Assuntos
Trifosfato de Adenosina/metabolismo , Fígado/metabolismo , Consumo de Oxigênio , Oxigênio/metabolismo , Animais , Transporte Biológico Ativo/efeitos dos fármacos , Transporte Biológico Ativo/fisiologia , Fármacos Cardiovasculares/farmacologia , Cobalto/farmacologia , Simulação por Computador , Difusão , Técnicas In Vitro , Fígado/efeitos dos fármacos , Masculino , Modelos Biológicos , Consumo de Oxigênio/efeitos dos fármacos , Ratos WistarRESUMO
It is convenient to study liver function and metabolism on the model of isolated perfused organ. The results of the present study indicate that viability and metabolic activity of the organ largely depend on the composition of the medium. Under conditions of isolated perfusion, the known pathway of oxygen transport through capillary filtration is supplemented by oxygen delivery to cells through the organ surface making an important contribution to liver oxygenation.
Assuntos
Meios de Cultura/química , Circulação Hepática/fisiologia , Fígado , Perfusão/métodos , Técnicas de Cultura de Tecidos , Animais , Feminino , Fígado/anatomia & histologia , Fígado/metabolismo , Consumo de Oxigênio , Ratos , Ratos WistarAssuntos
Fígado/metabolismo , Consumo de Oxigênio , Animais , Feminino , Técnicas In Vitro , Fígado/irrigação sanguínea , Perfusão , Ratos , Ratos WistarRESUMO
Haemopoietic stem cells content and proliferative activity were studied in the bone marrow of female F1 (CBA x C57Bl6) mice after single (50 mg/kg) and chronic (0.5 mg/kg daily for 7 days) serotonin (S) injections. It is shown that 9-day and 12-day COEs contents in the bone marrow of experimental mice has been increasing for 24 h after single S injection. After chronic S injections twofold increase of 12-day COEs is observed without any increasing of 9-day COEs. Total myelokaryocyte number is increased too. The study of proliferative status by in vitro incubation of bone marrow cells with ARA-C has shown that the numbers of 9-day and 12-day COEs in S-phase have increased both after single and chronic S injections. Possible mechanisms of stimulating effect of S on bone marrow stem cells are discussed.