Time-course study of genetic changes in periodontal ligament regeneration after tooth replantation in a mouse model.

This research focused on analyzing gene expression changes in the periodontal ligament (PDL) after tooth re-plantation to identify key genes and pathways involved in healing and regeneration. Utilizing a mouse model, mRNA was extracted from the PDL at various intervals post-replantation for RNA sequencing analysis, spanning from 3 to 56 days. The results revealed significant shifts in gene expression, particularly notable on day 28, supported by hierarchical clustering and principal component analysis. Gene ontology (GO) enrichment analysis highlighted an upregulation in olfactory receptor and G protein-coupled receptor signaling pathways at this time point. These findings were validated through reverse transcription-quantitative PCR (RT-qPCR), with immunochemical staining localizing olfactory receptor gene expression to the PDL and surrounding tissues. Moreover, a scratch assay indicated that olfactory receptor genes might facilitate wound healing in human PDL fibroblasts. These results underscore the importance of the 28-day post-transplant phase as a potential "tipping point" in PDL healing and regeneration. In conclusion, this research sheds light on the potential role of olfactory receptor genes in PDL regeneration, providing a foundation for developing new therapeutic approaches in tooth replantation and transplantation, with broader implications for regenerative medicine in oral health.

Antibacterial activities and mineral induction abilities of proprietary MTA cements.

Mineral trioxide aggregate (MTA) cements are used in direct pulp capping and many other applications, and several types of these products have been commercialized. The aim of this study was to examine the antibacterial effects and mineral induction abilities of three conventional MTA cements and one resin-modified MTA cement. Agar diffusion tests revealed that, after setting, all four cements exhibited little antibacterial effects against Enterococcus faecalis and Streptococcus mutans, with no significant differences among the materials. After 24 h, E. faecalis and S. mutans suspensions incubated in the presence of each cement did not exhibit reduced numbers of viable bacteria, compared with those same bacterial suspensions incubated without any cement; this indicated that none of the cements inhibited bacterial growth. Furthermore, the resin-modified MTA cement exhibited lower mineral induction ability, compared with that of the three conventional MTA cements.

Correlation between newborn size and gross fetal movement as counted by a fetal movement acceleration measurement recorder.

The development of the fetal movement acceleration measurement (FMAM) recorder has enabled the accurate counting of gross fetal movements. The aim of the study was to investigate whether gross fetal movement is related to a newborn's size. A total of 90 pregnant women who delivered singleton infant at term were recruited. Gross fetal movements were counted using an FMAM recorder during maternal sleep. The ratio of movement positive 10-s epochs to all epochs during one night was calculated as an index of fetal movement. Independent explanatory variables for the fetal movement index were selected from eight possibilities, that is, maternal age, gestational week, and the six physical measures of the newborn (height, weight, head circumference, chest circumference, Kaup index, and the ratio of head to chest circumference) with the stepwise regression procedure. The selected physical variables and the fetal movement index were analyzed using multiple regression analysis. A total of 2812.95 h from 423 night records were available. Gestational weeks and weight of the newborn were selected as the significant independent variables. Multiple regression analysis revealed that newborn weight had a positive correlation with the fetal movement index (p < 0.0001). The multiple regression equation was "The fetal movement index (%) = 34.9989-0.9088 × gestational weeks + 0.0033 × newborn weight (g)." A person's physical ability and lifetime activity level may originate from fetal health. This study may provide a new way of looking at the Developmental Origins of Health and Disease theory.

Counting fetal movements of small-for-gestational infants using a fetal movement acceleration measurement recorder.

Background: Our purpose was to clarify whether small-for-gestational (SGA) infants is associated with a decrease in fetal movements (FMs) even in the absence of hypoxia. We used a fetal movement acceleration measurement (FMAM) recorder, which enabled counting gross FMs for hours at a time.Methods: (1) Parameters of FMs for 13 women who delivered SGA infants were plotted over normal reference value curves made from 64 normal pregnant women in another study. (2) Linear regression analysis was conducted for the women with SGA infants and the normal pregnant women.Results: Thirty-eight data recordings were available in the SGA group. (1) For the ratio of movement positive 10-s epoch, 14 recordings (36.8%) were below 10% of the normal values. For an average number of movements, 13 (34.2%) were below 10%. Regarding average number, average duration, and longest duration of non-movement period, 12 (31.6%), 13 (34.2%), and 15 records (39.4%) were above 90% of the normal values, respectively. (2) SGA was a factor that decreased the positive epoch ratio and the average movements number, and increased the average number and duration, and the longest duration of non-movement period.Conclusions: SGA is associated with decreased movements even in the absence of hypoxia.

Correlation between umbilical cord length and gross fetal movement as counted by a fetal movement acceleration measurement recorder.

INTRODUCTION: The fetal movement acceleration measurement (FMAM) recorder has made it possible to count gross fetal movements over many hours. Our purpose was to examine the relationship between umbilical cord length and fetal movements as counted by the FMAM recorder. METHODS: Sixty-two pregnant women recorded fetal movements weekly from 28 weeks to term. The ratio of 10-s periods in which movement occurred to total time was calculated as a movement index. Umbilical cord length was measured at delivery. (1) Multiple linear regression analyses were conducted with six explanatory variables (primipara / multipara, anterior / posterior located placenta, placental weight, the mean movement index of 28-31, 32-35, and 36-39 week) and a response valuable (umbilical cord length). (2) All women were divided into groups of shorter, middle, and longer cord length, specifically less than 50 cm, between 50 and 60 cm, and more than 60 cm. The movement index was compared among the three groups at 28-31, 32-35, and 36-39 weeks. RESULTS: A total of 2355.6 h from 368 night records were available. (1) There were no relationships between the cord length and the movement index of 28-31, 32-35, and 36-39 weeks (p = 0.090, 0.235, 0.129, respectively). (2) There were no differences in the movement index among the three groups at 28-31 and 32-35 gestational weeks (p = 0.096, and 0.465, respectively); however, the longer cord group had a greater movement index than the other two groups at 36-39 weeks (p = 0.0008). DISCUSSION: This study suggested that fetal movement near term is an important factor in determining whether cord length becomes relatively longer in normal pregnancies.

Reference values for a fetal movement acceleration measurement recorder to count fetal movements.

BackgroundA newly developed fetal movement acceleration measurement recorder has made it possible to count gross movements for hours. The purpose of this study was to determine the normal reference values for such movements.MethodsOne hundred and six pregnant women recorded fetal movements by themselves when they slept at home weekly from 28 weeks to term. The normal reference values were determined based on the data that could be recorded for more than 4 h per night.ResultsA total of 2,458 h of data from 385 recordings from 64 women was available. The median ratio of 10-s periods in which fetal movements occurred to the total time interval was 17% at 28 gestational weeks, decreasing to ∼6% at term. The number of fetal movements was 74 times/h, decreasing to 29 times at term. The number, the mean, and the longest durations of periods with no fetal movement, meaning no fetal movements were found for more than 5 min, were 1.56 times/h, 7.95 and 14.25 min, respectively, at 28 weeks, and increasing to 2.54 times, and 9.63 and 19.67 min, respectively, at term.ConclusionsThis study provides normal reference values for gross fetal movement count using the fetal movement acceleration measurement recorder.

Counting fetal hiccups using a fetal movement acceleration measurement recorder.

OBJECTIVE: To clarify the degree of fetal hiccup occurrence by using a fetal movement acceleration measurement recorder. METHODS: A total of 23 pregnant women recorded fetal movements weekly or biweekly between 28 and 39 gestational weeks at home with the recorder. Fetal hiccups were defined as regular sharp oscillations - which occurred at 2-4 second intervals, more than 15 times per minute - on the maternal abdomen. The duration and frequency of the hiccup bouts were counted. The data were classified into an early (28-33 weeks) gestational group and a late (34-39 weeks) group, and compared between the two. RESULTS: A total of 164 records were obtained, and the total time analyzed amounted to 1035 hours. The mean incidence of a fetal hiccup bout at an early group was 0.19 times per hour, and it decreased to 0.15 at a late group (p = 0.02). The durations of fetal hiccup bouts were 8.17 and 7.88 minutes at an early and a late group, respectively, with no significant difference (p = 0.64). CONCLUSIONS: The duration of a fetal hiccup bout did not change after 28 gestational weeks; however, incidence slightly decreased from an early to a late group.

Changes in uterine flexion caused by cesarean section: correlation between post-flexion and deficient cesarean section scars.

PURPOSE: To investigate changes in uterine flexion after cesarean delivery in comparison with vaginal delivery, and their relationship with the presence of deficient cesarean section scars. METHODS: In 147 women who had a vaginal delivery and 101 women who had a cesarean delivery, transvaginal ultrasonographic photographs of the uterus were obtained at the first trimester of pregnancy and at 1 month postpartum, and they were reviewed to determine changes in uterine flexion. The presence of a deficient cesarean section scar was also reviewed in women with a cesarean section. RESULTS: Changes in uterine flexion were observed more frequently among the woman with a cesarean delivery than in those with a vaginal delivery. The incidence of post-flexed uterus during puerperium tended to increase depending on the number of cesarean sections the woman had undergone. In the women with a cesarean section, changes in uterine flexion were more frequently observed in the women with a deficient cesarean section scar than in those without one. CONCLUSION: Changes in uterine flexion after birth occurred more frequently in the women who had a cesarean delivery than in those who had a vaginal delivery, especially in the presence of a deficient cesarean section scar.