The Acute Demands of Repeated-Sprint Training on Physiological, Neuromuscular, Perceptual and Performance Outcomes in Team Sport Athletes: A Systematic Review and Meta-analysis.

BACKGROUND: Repeated-sprint training (RST) involves maximal-effort, short-duration sprints (≤ 10 s) interspersed with brief recovery periods (≤ 60 s). Knowledge about the acute demands of RST and the influence of programming variables has implications for training prescription. OBJECTIVES: To investigate the physiological, neuromuscular, perceptual and performance demands of RST, while also examining the moderating effects of programming variables (sprint modality, number of repetitions per set, sprint repetition distance, inter-repetition rest modality and inter-repetition rest duration) on these outcomes. METHODS: The databases Pubmed, SPORTDiscus, MEDLINE and Scopus were searched for original research articles investigating overground running RST in team sport athletes ≥ 16 years. Eligible data were analysed using multi-level mixed effects meta-analysis, with meta-regression performed on outcomes with ~ 50 samples (10 per moderator) to examine the influence of programming factors. Effects were evaluated based on coverage of their confidence (compatibility) limits (CL) against elected thresholds of practical importance. RESULTS: From 908 data samples nested within 176 studies eligible for meta-analysis, the pooled effects (± 90% CL) of RST were as follows: average heart rate (HRavg) of 163 ± 9 bpm, peak heart rate (HRpeak) of 182 ± 3 bpm, average oxygen consumption of 42.4 ± 10.1 mL·kg-1·min-1, end-set blood lactate concentration (B[La]) of 10.7 ± 0.6 mmol·L-1, deciMax session ratings of perceived exertion (sRPE) of 6.5 ± 0.5 au, average sprint time (Savg) of 5.57 ± 0.26 s, best sprint time (Sbest) of 5.52 ± 0.27 s and percentage sprint decrement (Sdec) of 5.0 ± 0.3%. When compared with a reference protocol of 6 × 30 m straight-line sprints with 20 s passive inter-repetition rest, shuttle-based sprints were associated with a substantial increase in repetition time (Savg: 1.42 ± 0.11 s, Sbest: 1.55 ± 0.13 s), whereas the effect on sRPE was trivial (0.6 ± 0.9 au). Performing two more repetitions per set had a trivial effect on HRpeak (0.8 ± 1.0 bpm), B[La] (0.3 ± 0.2 mmol·L-1), sRPE (0.2 ± 0.2 au), Savg (0.01 ± 0.03) and Sdec (0.4; ± 0.2%). Sprinting 10 m further per repetition was associated with a substantial increase in B[La] (2.7; ± 0.7 mmol·L-1) and Sdec (1.7 ± 0.4%), whereas the effect on sRPE was trivial (0.7 ± 0.6). Resting for 10 s longer between repetitions was associated with a substantial reduction in B[La] (-1.1 ± 0.5 mmol·L-1), Savg (-0.09 ± 0.06 s) and Sdec (-1.4 ± 0.4%), while the effects on HRpeak (-0.7 ± 1.8 bpm) and sRPE (-0.5 ± 0.5 au) were trivial. All other moderating effects were compatible with both trivial and substantial effects [i.e. equal coverage of the confidence interval (CI) across a trivial and a substantial region in only one direction], or inconclusive (i.e. the CI spanned across substantial and trivial regions in both positive and negative directions). CONCLUSIONS: The physiological, neuromuscular, perceptual and performance demands of RST are substantial, with some of these outcomes moderated by the manipulation of programming variables. To amplify physiological demands and performance decrement, longer sprint distances (> 30 m) and shorter, inter-repetition rest (≤ 20 s) are recommended. Alternatively, to mitigate fatigue and enhance acute sprint performance, shorter sprint distances (e.g. 15-25 m) with longer, passive inter-repetition rest (≥ 30 s) are recommended.

δ13 C values of urinary 19-norandrosterone in antidoping samples and potential for adverse findings from boar offal consumption.

19-Norandrosterone (19NA) is the preferred urinary target compound to identify doping with nandrolone or related 19-norsteroids. At concentrations between 2.5 and 15 ng/mL, isotope ratio mass spectrometry (IRMS) is required to establish exogenous origin of urinary 19NA. An absolute difference of 3‰ between urinary 19NA and an endogenous reference compound (ERC) constitutes a finding for exogenous origin of 19NA. Over the last 3 years, 77 samples containing urinary 19NA between 2.5 and 15 ng/mL were analyzed at our laboratory. The measured δ13 C values for 19NA ranged from -29.5‰ to -16.8‰. In comparison, the δ13 C values for the corresponding urinary ERCs ranged from -22.4‰ to -16.2‰. Due to the considerable overlap in values between the target compound and the natural range of urinary ERCs, it can be challenging to distinguish between endogenous and exogenous origins of urinary 19NA. In addition, it is well known that consumption of offal from non-castrated pigs can produce 19NA in urine. To determine whether this could cause a positive IRMS finding under the current IRMS positivity criteria, meat from non-castrated boars fed a mixture of corn and soy was consumed by 13 volunteers. Two volunteers produced 19NA findings above 2.5 ng/mL, and the measured isotope values, while inconsistent with documented 19-norsteroid preparations, did meet IRMS positivity criteria. However, these increases in 19NA urinary concentrations were short-lived due to rapid elimination. Timely follow-up collections may help support a claim for dietary exposure when low urinary concentrations of 19NA with pseudo-endogenous isotope values are observed.

Confronting ethical and social issues related to the genetics of musicality.

New interdisciplinary research into genetic influences on musicality raises a number of ethical and social issues for future avenues of research and public engagement. The historical intersection of music cognition and eugenics heightens the need to vigilantly weigh the potential risks and benefits of these studies and the use of their outcomes. Here, we bring together diverse disciplinary expertise (complex trait genetics, music cognition, musicology, bioethics, developmental psychology, and neuroscience) to interpret and guide the ethical use of findings from recent and future studies. We discuss a framework for incorporating principles of ethically and socially responsible conduct of musicality genetics research into each stage of the research lifecycle: study design, study implementation, potential applications, and communication.

Effect of Traditional, Rest Redistribution, and Velocity-Based Prescription on Repeated Sprint Training Performance and Responses in Semiprofessional Athletes.

ABSTRACT: Weakley, J, Castilla, AP, Ramos, AG, Banyard, H, Thurlow, F, Edwards, T, Morrison, M, McMahon, E, and Owen, C. The effect of traditional, rest redistribution, and velocity-based prescription on repeated sprint training performance and responses in semi-professional athletes. J Strength Cond Res 37(8): 1566-1572, 2023-The aim of this study was to investigate the effects of traditional, rest redistribution, and velocity-based repeated sprint training methods on repeated sprint performance, perceived effort, heart rate, and changes in force-velocity-power (FVP) profiles in male semiprofessional athletes. In a randomized crossover design, a traditional (2 sets of 6 repetitions [TRAD]), 2 different rest redistribution (4 sets of 3 repetitions [RR4] and 12 sets of 1 repetition [RR12]), and a 5% velocity loss (VL5%) (12 repetitions, with sets terminated when a 5% reduction in mean velocity had occurred) condition were completed. Mean and peak velocity, mean heart rate, and differential ratings of perceived exertion (dRPE) were measured throughout each session, while horizontal FVP profiles were assessed presession and postsession. The RR4 and RR12 conditions allowed the greatest maintenance of velocity, while the RR4, RR12, and VL5% had a moderate , significantly greater mean heart rate than the traditional condition. Trivial , nonsignificant differences between all conditions were observed in dRPE of the legs and breathlessness and FVP profiles. These findings indicate that rest redistribution can allow for greater maintenance of sprint velocity and heart rate, without altering perceived effort during repeated sprint training. In addition, velocity-loss thresholds may be a feasible method of prescription if athletes have diverse physical qualities and reductions in sprint performance during repeated sprint training are undesirable. Practitioners should consider these outcomes when designing repeated sprint training sessions because the strategic use of these methods can alter sprint performance and internal load without changing perceptions of intensity.

Comparison of Sprint Timing Methods on Performance, and Displacement and Velocity at Timing Initiation.

ABSTRACT: Weakley, J, McCosker, C, Chalkley, D, Johnston, R, Munteanu, G, and Morrison, M. Comparison of sprint timing methods on performance, and displacement and velocity at timing initiation. J Strength Cond Res 37(1): 234-238, 2023-Sprint testing is commonly used to assess speed and acceleration in athletes. However, vastly different outcomes have been reported throughout the literature. These differences are likely due to the sprint timing method rather than differences in athlete ability. Consequently, this study compared different sprint starting methods on sprint time and quantified the velocity and displacement of the athlete at the moment timing is initiated. Starting in a staggered 2-point stance, 12 team sport athletes were required to accelerate 10 meters for 10 repetitions. During each repetition, 5 independent timing methods were triggered. The methods were (a) triggering a Move sensor; (b) starting 50 cm behind the line; (c) triggering a front-foot switch; (d) triggering a rear-foot switch; and (e) starting with the front foot on the line. Timing for each method was initiated at different points during the acceleration phase, and the displacement and velocity of the centroid of the pelvis at the point of timing initiation was assessed under high-speed motion capture. The Move sensor had the smallest displacement and lowest velocity at the point of timing initiation, whereas the front-foot trigger demonstrated the largest displacement and highest velocities. Trivial to very large effect size differences were observed between all methods in displacement and velocity at the point of timing initiation. Furthermore, small to very large differences in time to 5 m were found. These findings emphasize that sprint outcomes should not be compared, unless starting methods are identical. In addition, to detect real change in performance, consistent standardized protocols should be implemented.

The Criterion Validity and Between-Day Reliability of the Perch for Measuring Barbell Velocity During Commonly Used Resistance Training Exercises.

ABSTRACT: Weakley, J, Munteanu, G, Cowley, N, Johnston, R, Morrison, M, Gardiner, C, Pérez-Castilla, A, and García-Ramos, A. The criterion validity and between-day reliability of the Perch for measuring barbell velocity during commonly used resistance training exercises. J Strength Cond Res 37(4): 787-792, 2023-This study aimed to assess the criterion validity and between-day reliability (accounting for technological and biological variability) of mean and peak concentric velocity from the Perch measurement system. On 2 testing occasions, 16 subjects completed repetitions at 20, 40, 60, 80, 90, and 100% of 1-repetition maximum in the free-weight barbell back squat and bench press. To assess criterion validity, values from the Perch and a 3-dimensional motion capture system (criterion) were compared. Technological variability was assessed by determining whether the differences between the Perch and criterion for each load were comparable for both testing sessions, whereas between-day reliability with both technological and biological variability was calculated from Perch values across days. Generalized estimating equations were used to calculate R2 and root mean square error, whereas Bland-Altman plots assessed magnitude of difference between measures. To support monitoring of athletes over time, standard error of measurement and minimum detectable changes (MDC) were calculated. There was excellent agreement between the Perch and criterion device, with mean velocity in both exercises demonstrating a mean bias ranging from -0.01 to 0.01 m·s -1 . For peak velocity, Perch underestimated velocity compared with the criterion ranging from -0.08 to -0.12 m·s -1 for the back squat and -0.01 to -0.02 m·s -1 for the bench press. Technological variability between-days were all less than the MDC. These findings demonstrate that the Perch provides valid and reliable mean and peak concentric velocity outputs across a range of velocities. Therefore, practitioners can confidently implement this device for the monitoring and prescription of resistance training.

PRELP Regulates Cell-Cell Adhesion and EMT and Inhibits Retinoblastoma Progression.

Retinoblastoma (RB) is the most common intraocular pediatric cancer. Nearly all cases of RB are associated with mutations compromising the function of the RB1 tumor suppressor gene. We previously demonstrated that PRELP is widely downregulated in various cancers and our in vivo and in vitro analysis revealed PRELP as a novel tumor suppressor and regulator of EMT. In addition, PRELP is located at chromosome 1q31.1, around a region hypothesized to be associated with the initiation of malignancy in RB. Therefore, in this study, we investigated the role of PRELP in RB through in vitro analysis and next-generation sequencing. Immunostaining revealed that PRELP is expressed in Müller glial cells in the retina. mRNA expression profiling of PRELP-/- mouse retina and PRELP-treated RB cells found that PRELP contributes to RB progression via regulation of the cancer microenvironment, in which loss of PRELP reduces cell-cell adhesion and facilitates EMT. Our observations suggest that PRELP may have potential as a new strategy for RB treatment.

Sleep, circadian biology and skeletal muscle interactions: Implications for metabolic health.

There currently exists a modern epidemic of sleep loss, triggered by the changing demands of our 21st century lifestyle that embrace 'round-the-clock' remote working hours, access to energy-dense food, prolonged periods of inactivity, and on-line social activities. Disturbances to sleep patterns impart widespread and adverse effects on numerous cells, tissues, and organs. Insufficient sleep causes circadian misalignment in humans, including perturbed peripheral clocks, leading to disrupted skeletal muscle and liver metabolism, and whole-body energy homeostasis. Fragmented or insufficient sleep also perturbs the hormonal milieu, shifting it towards a catabolic state, resulting in reduced rates of skeletal muscle protein synthesis. The interaction between disrupted sleep and skeletal muscle metabolic health is complex, with the mechanisms underpinning sleep-related disturbances on this tissue often multifaceted. Strategies to promote sufficient sleep duration combined with the appropriate timing of meals and physical activity to maintain circadian rhythmicity are important to mitigate the adverse effects of inadequate sleep on whole-body and skeletal muscle metabolic health. This review summarises the complex relationship between sleep, circadian biology, and skeletal muscle, and discusses the effectiveness of several strategies to mitigate the negative effects of disturbed sleep or circadian rhythms on skeletal muscle health.

Design-development of an at-home modular brain-computer interface (BCI) platform in a case study of cervical spinal cord injury.

OBJECTIVE: The objective of this study was to develop a portable and modular brain-computer interface (BCI) software platform independent of input and output devices. We implemented this platform in a case study of a subject with cervical spinal cord injury (C5 ASIA A). BACKGROUND: BCIs can restore independence for individuals with paralysis by using brain signals to control prosthetics or trigger functional electrical stimulation. Though several studies have successfully implemented this technology in the laboratory and the home, portability, device configuration, and caregiver setup remain challenges that limit deployment to the home environment. Portability is essential for transitioning BCI from the laboratory to the home. METHODS: The BCI platform implementation consisted of an Activa PC + S generator with two subdural four-contact electrodes implanted over the dominant left hand-arm region of the sensorimotor cortex, a minicomputer fixed to the back of the subject's wheelchair, a custom mobile phone application, and a mechanical glove as the end effector. To quantify the performance for this at-home implementation of the BCI, we quantified system setup time at home, chronic (14-month) decoding accuracy, hardware and software profiling, and Bluetooth communication latency between the App and the minicomputer. We created a dataset of motor-imagery labeled signals to train a binary motor imagery classifier on a remote computer for online, at-home use. RESULTS: Average bluetooth data transmission delay between the minicomputer and mobile App was 23 ± 0.014 ms. The average setup time for the subject's caregiver was 5.6 ± 0.83 min. The average times to acquire and decode neural signals and to send those decoded signals to the end-effector were respectively 404.1 ms and 1.02 ms. The 14-month median accuracy of the trained motor imagery classifier was 87.5 ± 4.71% without retraining. CONCLUSIONS: The study presents the feasibility of an at-home BCI system that subjects can seamlessly operate using a friendly mobile user interface, which does not require daily calibration nor the presence of a technical person for at-home setup. The study also describes the portability of the BCI system and the ability to plug-and-play multiple end effectors, providing the end-user the flexibility to choose the end effector to accomplish specific motor tasks for daily needs. Trial registration ClinicalTrials.gov: NCT02564419. First posted on 9/30/2015.

A Systematic Review on Fitness Testing in Adult Male Basketball Players: Tests Adopted, Characteristics Reported and Recommendations for Practice.

BACKGROUND: As basketball match-play requires players to possess a wide range of physical characteristics, many tests have been introduced in the literature to identify talent and quantify fitness in various samples of players. However, a synthesis of the literature to identify the most frequently used tests, outcome variables, and normative values for basketball-related physical characteristics in adult male basketball players is yet to be conducted. OBJECTIVE: The primary objectives of this systematic review are to (1) identify tests and outcome variables used to assess physical characteristics in adult male basketball players across all competition levels, (2) report a summary of anthropometric, muscular power, linear speed, change-of-direction speed, agility, strength, anaerobic capacity, and aerobic capacity in adult male basketball players based on playing position and competition level, and (3) introduce a framework outlining recommended testing approaches to quantify physical characteristics in adult male basketball players. METHODS: A systematic review of MEDLINE, PubMed, SPORTDiscus, Scopus, and Web of Science was performed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines to identify relevant studies. To be eligible for inclusion, studies were required to: (1) be original research articles; (2) be published in a peer-reviewed journal; (3) have full-text versions available in the English language; and (4) include the primary aim of reporting tests used and/or the physical characteristics of adult (i.e., ≥ 18 years of age) male basketball players. Additionally, data from the top 10 draft picks who participated in the National Basketball Association combined from 2011-12 to 2020-21 were extracted from the official league website to highlight the physical characteristics of elite 19- to 24-year-old basketball players. RESULTS: A total of 1684 studies were identified, with 375 being duplicates. Consequently, the titles and abstracts of 1309 studies were screened and 231 studies were eligible for full-text review. The reference list of each study was searched, with a further 59 studies identified as eligible for review. After full-text screening, 137 studies identified tests, while 114 studies reported physical characteristics in adult male basketball players. CONCLUSIONS: Physical characteristics reported indicate a wide range of abilities are present across playing competitions. The tests and outcome variables reported in the literature highlight the multitude of tests currently being used. Because there are no accepted international standards for physical assessment of basketball players, establishing normative data is challenging. Therefore, future testing should involve repeatable protocols that are standardised and provide outcomes that can be monitored across time. Recommendations for testing batteries in adult male basketball players are provided so improved interpretation of data can occur. CLINICAL TRIAL REGISTRATION: This review was registered with the International Prospective Register of Systematic Reviews and allocated registration number CRD42020187151 on 28 April, 2020.

Putting the Squeeze on Compression Garments: Current Evidence and Recommendations for Future Research: A Systematic Scoping Review.

BACKGROUND: Compression garments are regularly worn during exercise to improve physical performance, mitigate fatigue responses, and enhance recovery. However, evidence for their efficacy is varied and the methodological approaches and outcome measures used within the scientific literature are diverse. OBJECTIVES: The aim of this scoping review is to provide a comprehensive overview of the effects of compression garments on commonly assessed outcome measures in response to exercise, including: performance, biomechanical, neuromuscular, cardiovascular, cardiorespiratory, muscle damage, thermoregulatory, and perceptual responses. METHODS: A systematic search of electronic databases (PubMed, SPORTDiscus, Web of Science and CINAHL Complete) was performed from the earliest record to 27 December, 2020. RESULTS: In total, 183 studies were identified for qualitative analysis with the following breakdown: performance and muscle function outcomes: 115 studies (63%), biomechanical and neuromuscular: 59 (32%), blood and saliva markers: 85 (46%), cardiovascular: 76 (42%), cardiorespiratory: 39 (21%), thermoregulatory: 19 (10%) and perceptual: 98 (54%). Approximately 85% (n = 156) of studies were published between 2010 and 2020. CONCLUSIONS: Evidence is equivocal as to whether garments improve physical performance, with little evidence supporting improvements in kinetic or kinematic outcomes. Compression likely reduces muscle oscillatory properties and has a positive effect on sensorimotor systems. Findings suggest potential increases in arterial blood flow; however, it is unlikely that compression garments meaningfully change metabolic responses, blood pressure, heart rate, and cardiorespiratory measures. Compression garments increase localised skin temperature and may reduce perceptions of muscle soreness and pain following exercise; however, rating of perceived exertion during exercise is likely unchanged. It is unlikely that compression garments negatively influence exercise-related outcomes. Future research should assess wearer belief in compression garments, report pressure ranges at multiple sites as well as garment material, and finally examine individual responses and varying compression coverage areas.

The Validity and Reliability of Commercially Available Resistance Training Monitoring Devices: A Systematic Review.

BACKGROUND: Monitoring resistance training has a range of unique difficulties due to differences in physical characteristics and capacity between athletes, and the indoor environment in which it often occurs. Traditionally, methods such as volume load have been used, but these have inherent flaws. In recent times, numerous portable and affordable devices have been made available that purport to accurately and reliably measure kinetic and kinematic outputs, potentially offering practitioners a means of measuring resistance training loads with confidence. However, a thorough and systematic review of the literature describing the reliability and validity of these devices has yet to be undertaken, which may lead to uncertainty from practitioners on the utility of these devices. OBJECTIVE: A systematic review of studies that investigate the validity and/or reliability of commercially available devices that quantify kinetic and kinematic outputs during resistance training. METHODS: Following PRISMA guidelines, a systematic search of SPORTDiscus, Web of Science, and Medline was performed; studies included were (1) original research investigations; (2) full-text articles written in English; (3) published in a peer-reviewed academic journal; and (4) assessed the validity and/or reliability of commercially available portable devices that quantify resistance training exercises. RESULTS: A total of 129 studies were retrieved, of which 47 were duplicates. The titles and abstracts of 82 studies were screened and the full text of 40 manuscripts were assessed. A total of 31 studies met the inclusion criteria. Additional 13 studies, identified via reference list assessment, were included. Therefore, a total of 44 studies were included in this review. CONCLUSION: Most of the studies within this review did not utilise a gold-standard criterion measure when assessing validity. This has likely led to under or overreporting of error for certain devices. Furthermore, studies that have quantified intra-device reliability have often failed to distinguish between technological and biological variability which has likely altered the true precision of each device. However, it appears linear transducers which have greater accuracy and reliability compared to other forms of device. Future research should endeavour to utilise gold-standard criterion measures across a broader range of exercises (including weightlifting movements) and relative loads.

Velocity Loss Thresholds Reliably Control Kinetic and Kinematic Outputs during Free Weight Resistance Training.

Exercise velocity and relative velocity loss thresholds (VLTs) are commonly used in velocity-based resistance training. This study aims to quantify the between-day reliability of 10%, 20%, and 30% VLTs on kinetic and kinematic outputs, changes in external load, and repetition characteristics in well-trained athletes. Using a repeated, counter-balanced crossover design, twelve semi-professional athletes completed five sets of the back squat with an external load corresponding to a mean concentric velocity of ~0.70 m·s-1 and a VLT applied. The testing sessions were repeated after four weeks of unstructured training to assess the long-term reliability of each VLT. A coefficient of variation (CV) <10% was used to classify outputs as reliable. Kinetic and kinematic outputs and external load were largely reliable, with only peak power during sets 2-5 within the 10% VLT condition demonstrating a CV >10% (CV: 11.14-14.92%). Alternatively, the repetitions completed within each set showed large variation (CV: 18.92-67.49%). These findings demonstrate that by utilizing VLTs, kinetic and kinematic outputs can be prescribed and replicated across training mesocycles. Thus, for practitioners wishing to reliably control the kinetic and kinematic stimulus that is being applied to their athletes, it is advised that a velocity-based approach is used.

Evaluation of epiandrosterone as a long-term marker of testosterone use.

Identification and evaluation of long-term markers is crucial in prolonging the detection window for anabolic steroid abuse in sport. Recently, sulfoconjugated epiandrosterone was identified as a potential long-term marker for the abuse of certain endogenous anabolic agents, including testosterone, which continues to be widely used as a performance enhancing agent in sport. To evaluate the applicability of epiandrosterone sulfate as a marker for testosterone use, administration studies were conducted with multiple modes of testosterone administration - transdermal, intramuscular, and subcutaneous. A modified sample preparation method was used to collect both glucuronidated and sulfoconjugated analytes of interest. Carbon isotope ratio measurements from the administration studies are presented here. Epiandrosterone was less effective than the conventionally used target compounds for detection of the low dose application (transdermal gel). With intramuscular administration, epiandrosterone was more diagnostic than with transdermal administration, but it did not prolong the detection window more than the conventional target compounds. With subcutaneous administration, the doses administered to the subjects were varied and the effect on the epiandrosterone values was dependent on the magnitude of the dose administered. Epiandrosterone does not appear to be a useful marker in the detection of low dose testosterone administration. It is responsive to higher dose administration, but it does not provide an extension of the detection window relative to conventional target compounds.

Criterion Validity, and Interunit and Between-Day Reliability of the FLEX for Measuring Barbell Velocity During Commonly Used Resistance Training Exercises.

Weakley, J, Chalkley, D, Johnston, R, García-Ramos, A, Townshend, A, Dorrell, H, Pearson, M, Morrison, M, and Cole, M. Criterion validity, and interunit and between-day reliability of the FLEX for measuring barbell velocity during commonly used resistance training exercises. J Strength Cond Res 34(6): 1519-1524, 2020-The aim of this study was to assess the criterion validity, interunit reliability (accounting for technological and biological variance), and between-day reliability of a novel optic laser device (FLEX) for quantifying mean concentric velocity. To assess the validity against a three-dimensional motion capture system and interunit reliability with both technological and biological variation, 18 men and women completed repetitions at 20, 40, 60, 80, 90, and 100% of one repetition maximum in the free-weight barbell back squat and bench press. To assess interunit (technological only) reliability, a purpose-built, calibrated rig completed a set protocol with 2 devices. To assess between-day reliability of the technology, the same protocol was repeated 21 days later. Standardized bias, typical error of the estimate (TEE; %), and Pearson's correlation coefficient (r) were used to assess validity, whereas typical error and coefficient of variation (CV%) were calculated for reliability. Overall, TEE (±90 CL) between the FLEX and criterion measure was 0.03 (±0.004) and 0.04 (±0.005) m·s in the back squat and bench press, respectively. For measures of reliability, overall interunit technological variance (CV% [± 90% confidence interval]) was 3.96% (3.83-4.12) but increased to 9.82% (9.31-10.41) and 9.83% (9.17-10.61) in the back squat and bench press, respectively, when biological variance was introduced. Finally, the overall between-day reliability was 3.77% (3.63-3.91). These findings demonstrate that the FLEX provides valid and reliable mean concentric velocity outputs across a range of velocities. Thus, practitioners can confidently implement this device for the monitoring and prescription of resistance training loads.

Male mice adjust courtship behavior in response to female multimodal signals.

Multimodal signaling is nearly ubiquitous across animal taxa. While much research has focused on male signal production contributing to female mate-choice or preferences, females often give their own multimodal signals during intersexual communication events. Multimodal signal components are often classified based on whether they contain redundant information (e.g., the backup hypothesis) or non-redundant information (e.g., the multiple messages hypothesis) from the perspective of the receiver. We investigated the role of two different female vocalizations produced by the female house mouse (Mus musculus): the broadband, relatively low-frequency squeaks (broadband vocalizations or BBVs,), and the higher-frequency ultrasonic vocalizations (USVs). These female vocalizations may convey differently valenced information to the male receivers. We paired these vocalizations with and without female urine to examine the influence of combining information across multiple modalities. We found evidence that female urine and vocalizations act as non-redundant multimodal cues as males responded with different behaviors and vocalization rates depending on the female signal presented. Additionally, male mice responded with greater courtship effort to the multimodal combination of female USVs paired with female urine than any other signal combination. These results suggest that the olfactory information contained in female urine provides the context by which males can then evaluate potentially ambiguous female vocalizations.

Retrospective analysis of a gabapentin high dose taper compared to lorazepam in acute inpatient alcohol withdrawal.

Background: Benzodiazepines have remained the standard of care for alcohol withdrawal syndrome; however, they have numerous unfavorable physiologic effects. Gabapentin has limited data to support a benefit in reducing benzodiazepine usage in alcohol withdrawal syndrome.Objectives: Evaluate the association of an institutional guideline and order set for alcohol withdrawal that incorporates high dose gabapentin tapers in acutely withdrawing patients. Methods: This retrospective study evaluated patients experiencing acute alcohol withdrawal. Two time periods were evaluated: a pre-protocol group assessed outcomes prior to implementation of a gabapentin backbone taper-based guideline (PRE-implementation), and a post-protocol group assessed post-guideline and order set introduction (POST-implementation). A total of 100 patients (50 PRE-implementation and 50 POST-implementation; 84% male, 16% female) were included in the analysis. Results: There was a significant difference in the median daily lorazepam usage in the PRE-implementation versus POST-implementation groups (9.48 [5.58-28.46] vs 6.52 [3.84-11.65] mg, P = 0.024) with a reduction observed in the POST-implementation group. There was also a significant difference in the mean hospital length of stay (LOS) in the PRE-implementation versus POST-implementation groups (9.92 ± 7.33 vs 7.04 ± 4.59 days, P = 0.021) in favor of the POST-implementation group. There was no difference in the number of rapid responses called, median intensive care unit (ICU) LOS, median number of days the patient was confusion assessment method for the ICU (CAM-ICU) positive or number of transfers to a higher level of care. Conclusions: Implementation of an institutional guideline and order set for alcohol withdrawal incorporating high dose gabapentin tapers was associated with a decreased median daily lorazepam use as well as hospital LOS; however, retrospective design and inherent limitations require larger prospective trials to validate results.

Identifying Candidate Biomarkers for Pleomorphic Adenoma: A Case-Control Study.

Pleomorphic adenoma (PA) is the most common benign salivary gland tumor. Kallikrein-related peptidases have been identified as biomarkers in many human tumors and may influence tumor behavior. We investigated KLK1-15 messenger ribonucleic acid and proteins in PA specimens to determine a KLK expression profile for this tumor. Fresh frozen PA tissue specimens (n = 26) and matched controls were subjected to quantitative real-time reverse transcription polymerase chain reaction to detect KLK1-15 mRNA. Expression of KLK1, KLK12, KLK13, and KLK8 proteins were then evaluated via immunostaining techniques. Statistical analyses were performed with the level of significance set at P < .05. We observed downregulation of KLK1, KLK12, and KLK13 mRNA expression, and immunostaining studies revealed downregulation of the corresponding proteins. Histologic evidence of capsular perforation was associated with increased KLK1 protein expression. Tumor size was not associated with capsular invasion and/or perforation. This study is the first to detail a KLK expression profile for PA at both the transcriptional level and the protein level. Future work is required to develop clinical applications of these findings.

Positron Emission Tomography Imaging of Macrophages in Atherosclerosis with 18F-GE-180, a Radiotracer for Translocator Protein (TSPO).

Intraplaque inflammation plays an important role in the progression of atherosclerosis. The 18 kDa translocator protein (TSPO) expression is upregulated in activated macrophages, representing a potential target to identify inflamed atherosclerotic plaques. We preclinically evaluated 18F-GE-180, a novel third-generation TSPO radioligand, in a mouse model of atherosclerosis. Methods. Nine hypercholesterolemic mice deficient in low density lipoprotein receptor and apolipoprotein B48 (LDLR-/-ApoB100/100) and six healthy C57BL/6N mice were injected with 10 MBq of 18F-GE-180. Specificity of binding was demonstrated in three LDLR-/-ApoB100/100 mice by injection of nonradioactive reference compound of 18F-GE-180 before 18F-GE-180. Dynamic 30-minute PET was performed followed by contrast-enhanced CT, and the mice were sacrificed at 60 minutes after injection. Tissue samples were obtained for ex vivo biodistribution measurements, and aortas were cut into serial cryosections for digital autoradiography. The presence of macrophages and TSPO was studied by immunohistochemistry. The 18F-GE-180 retention in plaque areas with different macrophage densities and lesion-free vessel wall were compared. Results. The LDLR-/-ApoB100/100 mice showed large, inflamed plaques in the aorta. Autoradiography revealed significantly higher 18F-GE-180 retention in macrophage-rich plaque areas than in noninflamed areas (count densities 150 ± 45 PSL/mm2 versus 51 ± 12 PSL/mm2, p < 0.001). Prominent retention in the vessel wall without plaque was also observed (220 ± 41 PSL/mm2). Blocking with nonradioactive GE-180 diminished the difference in count densities between macrophage-rich and noninflamed areas in atherosclerotic plaques and lowered the count density in vessel wall without plaque. Conclusion. 18F-GE-180 shows specific uptake in macrophage-rich areas of atherosclerotic plaques in mice. However, retention in atherosclerotic lesions does not exceed that in lesion-free vessel wall. The third-generation TSPO radioligand 18F-GE-180 did not show improved characteristics for imaging atherosclerotic plaque inflammation compared to previously studied TSPO-targeting tracers.