Neuromuscular system of the causative agent of dicrocoeliosis, Dicrocoelium lanceatum. I. 5-Hydroxytryptamine in the nervous system.

The trematode Dicrocoelium lanceatum known as lancet fluke, is a causative agent of dicrocoeliosis, a widespread parasitic disease of the grazing ruminants. The investigation of the major neurotransmitters and their functions are an important step in the development of a new pharmacological strategy of the struggle against the dicrocoeliosis affecting the neuronal signal substances and the functions of its nervous system. The aim of this work was to study the presence and localization of the neurotransmitter serotonin (5-HT, 5-Hydroxytryptamine) in the nervous system of D. lanceatum using immunocytochemical technique and confocal laser scanning microscopy. For the first time the data on the presence and distribution of serotonin-immunopositive components in the central and peripheral compartments of the nervous system of D. lanceatum has been obtained. Serotonin-immunopositive neurons and neurites were identified in paired brain ganglia, in the brain commissure, longitudinal nerve cords and connective nerve commissures. The innervation of the oral and ventral suckers by serotonergic nerve structures was revealed. The distal part of the reproductive system and the region of the reproductive pore were intensively innervated by serotonergic neurites. Serotonin-immunopositive neurons and neurites were also revealed in the proximal region of the reproductive system. The data obtained suggest that the serotonergic nervous system is involved in the regulation of the attachment organs and the reproductive system functions in D. lanceatum. The new results on the morphological and functional organization of the D. lanceatum nervous system increase our knowledge of the structure and function of nervous system of trematodes of various taxonomic groups and support the possibility of the exploitation of the serotonergic system of the parasite as a target for anthelmintic drugs.

The New Data on the Serotonin and FMRFamide Localization in the Nervous System of Opisthorchis felineus Metacercaria.

BACKGROUND: Trematoda Opisthorchis felineus Rivolta, 1884 is the causative agent of dangerous parasite disease-opisthorchiasis, widespread in the Russian Federation. The details of the neuroanatomical localization of the serotoninergic and FMRFamidergic neurotransmitter elements as well as their functional roles remain not studied enough in both adult and larval forms of O. felineus. The studies in this area are important in term of the development of a new pharmacological strategy of the struggle with the causative agent of opisthorchiasis affecting the neuronal signal substances and the function of its nervous system. PURPOSE: The aim of this work was the immunocytochemical study of the neurotransmitters serotonin (5-HT, 5-Hydroxitryptamine) and neuropeptide FMRFamide localization in the nervous system of the opisthorchiasis causative agent-O. felineus metacercaria. To study the relationship between the detected neurotransmitters and the muscular elements of the parasite, the muscle staining was carried out simultaneously using fluorophore-conjugated phalloidin. METHODS: The localization of 5-HTergic and FMRFamidergic nerve structures was determined by immunocytochemical method. The staining samples were analyzed using a fluorescent and confocal laser scanning microscopies. RESULTS: The new data on the presence and distribution of the serotonin-immunopositive (IP)- and FMRFa-IP components in the central and peripheral departments of the nervous system of  O. felineus metacercaria has been obtained. Besides that a number of the new anatomical details of the nervous system organization and of the innervation of the organs and tissues in the investigated parasite have been revealed. CONCLUSION: The data obtained on the presence and localization of the 5-HTergic and peptidergic (FMRFamide) components in central and peripheral departments of the nervous system of O. felineus metacercaria elaborated and expanded the existing information about the nervous system as well as the innervations of the tissues and organs in the causative agent of opistchorchiasis.

Serotonin and Neuropeptide FMRFamide in the Attachment Organs of Trematodes.

The serotoninergic and FMRFamidergic nervous system of the attachment organs of trematodes were examined using immunocytochemical techniques and confocal scanning laser microscopy. Adult trematodes from eight families as well as cercariae and metacercariae from ten families were studied. TRITC-conjugated phalloidin was used to stain the muscle fibres. The serotonin- and FMRFamide-immunoreactive (IR) nerve cells and fibres were revealed to be near the muscle fibres of the oral and ventral suckers of the trematodes and their larvae. The results indicate the important role of neurotransmitters, serotonin and neuropeptide FMRFamide in the regulation of muscle activity in the attachment organs of trematodes and can be considered in perspective for the development of new anthelmintic drugs, which can interrupt the function of the attachment organs of the parasites.