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1.
Jpn J Infect Dis ; 76(1): 55-63, 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36184398

RESUMO

Mosquitoes are important arthropod vectors of arboviruses. The family Phenuiviridae includes several medically important arboviruses, such as the Rift Valley fever phlebovirus and Toscana phlebovirus. Recent comprehensive genetic analyses have identified many novel mosquito-specific viruses that are phylogenetically related to Phenuiviridae. We collected mosquitoes from Hokkaido in northern Japan, and conducted reverse transcription polymerase chain reactions (RT-PCRs) targeting the RNA-dependent RNA polymerase (RdRp) gene of Phenuiviridae. A total of 285 pools, comprising 3,082 mosquitoes from 2 genera and 8 species, were collected. Partial RdRp sequences were detected in 97 pools, which allowed us to classify the viruses into 3 clusters provisionally designated as Etutanne virus (ETTV) 1, 2, and 3. The virus most closely related to ETTVs is Narangue virus (family Phenuiviridae, genus Mobuvirus), which was detected in Mansonia mosquitoes; the nucleotide and amino acid sequences of the Narangue virus are 58.4-66.2% and 64.7-86.7% similar, respectively, to those of ETTVs. PCR and RT-PCR using DNA and RNase digestion methods showed that the ETTVs are RNA viruses that do not form non-retroviral integrated RNA virus sequences in the mosquito genome.


Assuntos
Aedes , Arbovírus , Phlebovirus , Vírus , Animais , Aedes/genética , Japão , Mosquitos Vetores/genética , Arbovírus/genética , Phlebovirus/genética , RNA Polimerase Dependente de RNA
2.
Insects ; 12(12)2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34940145

RESUMO

Species of the genus Spiroplasma are common within arthropods and plants worldwide. Mosquito-associated Spiroplasma spp. have been reported to show pathogenicity toward mosquitoes, which serve as vectors of several infectious diseases that have detrimental effects on public health. Although Spiroplasma spp. are expected to have potential use as biological vector-control tools, characteristics such as their distribution, host species, and cytopathogenic effects (CPEs) are not well understood. In this study, we isolated a Spiroplasma sp. from a female Aedes japonicus collected in Hokkaido, northern Japan. Phylogenetic analysis based on the 16S rRNA gene sequence indicated our isolate was closely related to S. cantharicola. We screened 103 mosquito pools consisting of 3 genera and 9 species, but only detected S. cantharicola in the first isolation. In an in vitro assay, our isolate grew well at 28 °C, but no propagation was observed at 37 °C. Furthermore, the isolate showed strong CPE on a mosquito-derived cultured cell line (C6/36), and its propagation slightly increased when co-cultured with C6/36 cells. To our knowledge, this is the third report of the isolation of S. cantharicola from mosquitoes and the first case in Asia. Our findings provide epidemiological data on S. cantharicola distribution in the region.

3.
Pathogens ; 10(8)2021 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-34451402

RESUMO

The Zika virus (ZIKV) is a rapidly expanding mosquito-borne virus that causes febrile illness in humans. Aedes aegypti and Ae. albopictus are the primary ZIKV vectors; however, the potential vector competence of other Aedes mosquitoes distributed in northern Japan (Palearctic ecozone) are not yet known. In this study, the susceptibility to Zika virus infection of three Aedes mosquitoes distributed in the main city of the northern Japan and their capacities as vectors for ZIKV were evaluated. Field-collected mosquitoes were fed ad libitum an infectious blood meal containing the ZIKV PRVABC59. The Zika virus was detected in the abdomen of Ae. galloisi and Ae. japonicus at 2-10 days post infection (PI), and from the thorax and head of Ae. galloisi at 10 days PI, resulting in 17.6% and 5.9% infection rates, respectively. The Zika virus was not detected from Ae. punctor at any time. Some northern Japanese Aedes could be suspected as vectors of ZIKV but the risk may be low when compared with major ZIKV vectors.

4.
Ticks Tick Borne Dis ; 11(4): 101438, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32299787

RESUMO

Chemical tick control is a major means of preventing East Coast fever (ECF), especially in sub-Saharan Africa. However, in southwestern Uganda, improper tick control practices have led to severe acaricide resistance. The objectives of this study were to determine the risk factors associated with tick infestation in dairy cattle and Theileria parva infection, and to generate evidence for the prioritization of effective countermeasures for tick control. A cross-sectional study was conducted in 30 farms in Mbarara District, and information on tick control practices and tick infestation were collected. Tick samples were collected from 13 farms to test tick acaricide efficacy. A total of 420 blood samples from calves to adults of exotic- and cross-breed dairy cattle were collected, and T. parva diagnosis via polymerase chain reaction was performed. All the 13 tick populations tested were resistant to deltamethrin (synthetic pyrethroid). Resistance to single-formulation organophosphate-chlorfenvinphos was 39 % (5/13); co-formulations (chlorpyrifos + cypermethrin), 69 % (9/13); and amitraz (amidine), 85 % (11/13). The overall prevalence of T. parva infection at the individual-level was 45.2 % (190/420, 95 % confidence interval (CI): 40.4-50.1), and that at the farm-level was 83 % (25/30, 95 %CI: 65-94). A good quality cattle crush was a preventive factor for tick infestation (odds ratio (OR): 0.32, 95 %CI: 0.15-0.63, p =  0.001). Well-managed acaricide storage (OR: 0.36, 95 %CI: 0.17-0.76, p =  0.008), and a good quality measuring cylinder for acaricide were preventive factors (OR: 0.32, 95 %CI: 0.11-0.93, p = 0.036) for T. parva infection. The risk factors for T. parva infection were a longer period of acaricide use of the same brand (OR: 1.06, 95 %CI: 1.01-1.10, p = 0.012), and a higher frequency (twice a week) of acaricide use rather than once a week (OR: 11.70, 95 %CI: 1.95-70.13, p = 0.007). These risk factors should be given high intervention priority in order to effectively control ticks and prevent T. parva infections in dairy farms. Teaching proper practices to dairy farmers and to technical staff should be used to overcome the severe challenge of acaricide resistance in Mbarara District.


Assuntos
Theileria parva/fisiologia , Theileriose/epidemiologia , Controle de Ácaros e Carrapatos , Infestações por Carrapato/veterinária , Animais , Bovinos , Estudos Transversais , Indústria de Laticínios , Feminino , Fatores de Risco , Theileriose/parasitologia , Theileriose/prevenção & controle , Infestações por Carrapato/epidemiologia , Infestações por Carrapato/parasitologia , Infestações por Carrapato/prevenção & controle , Uganda/epidemiologia
5.
J Vet Med Sci ; 82(4): 488-493, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32132316

RESUMO

A cross-sectional study was conducted to investigate the risk factors for sub-clinical mastitis (SCM) in Mbarara District, an intensive dairy production region of Uganda where hand-milking is dominant. In 30 farms, herd-level milking practices and SCM prevalence were studied. The SCM prevalences were 68.6% (417/608, 95% confidence interval (CI): 64.9-72.2%) and 39.2% (946/2,411, 37.3-41.2%) at the cow- and quarter-levels, respectively. A preventive factor for SCM was cow calmness at the end of milking (OR: 0.20, 95%CI: 0.05-0.79, P=0.021); a risk factor was rough teat-end (OR: 1.75, 95%CI: 1.14-2.68, P=0.011). Good cow hygiene was negatively associated with environmental mastitis (P=0.002). Appropriate hand-milking practices that avoid teat damage are expected to reduce SCM in Uganda.


Assuntos
Indústria de Laticínios/métodos , Mastite Bovina/epidemiologia , Criação de Animais Domésticos/métodos , Animais , Infecções Assintomáticas/epidemiologia , Bactérias/isolamento & purificação , Comportamento Animal , Bovinos , Estudos Transversais , Feminino , Glândulas Mamárias Animais , Mastite Bovina/etiologia , Mastite Bovina/microbiologia , Leite/microbiologia , Prevalência , Fatores de Risco , Uganda/epidemiologia
6.
J Vet Med Sci ; 82(2): 188-192, 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-31875599

RESUMO

East Coast fever is caused by Theileria parva, and poses serious concerns for dairy farmers owing to massive economic losses. In the current study, we compared three methods (DNA extraction kits, FTA-NaOH and FTA-TENT) of DNA extraction to identify the most economical and reliable method. A survey for T. parva prevalence was conducted in dairy cattle in Mbarara, Uganda. Cytochrome C oxidase subunit I (COI) and T. parva-p104 genes were amplified to compare the methods. FTA-NaOH-based polymerase chain reaction (PCR) yielded the best detection rate for both COI gene and p104 gene. Prevalence of T. parva was 45.0% and 83.3% at animal and farm-level, respectively. FTA-NaOH based-PCR is simple, highly sensitive and cost-effective tool for T. parva diagnosis in resource constrained settings.


Assuntos
Doenças dos Bovinos/diagnóstico , Reação em Cadeia da Polimerase/veterinária , Theileria parva/isolamento & purificação , Theileriose/diagnóstico , Animais , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/parasitologia , DNA/análise , DNA/isolamento & purificação , Indústria de Laticínios , Papel , Reação em Cadeia da Polimerase/economia , Reação em Cadeia da Polimerase/métodos , Prevalência , Hidróxido de Sódio , Manejo de Espécimes/instrumentação , Theileria parva/genética , Theileriose/sangue , Theileriose/epidemiologia , Uganda
7.
Zoonoses Public Health ; 66(8): 936-942, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31464049

RESUMO

Bergeyella zoohelcum causes rare but severe human clinical diseases, which mostly arise from animal bites. Notably, Bergeyella infections can also occur in older people after prolonged exposure to dogs or cats without biting. We detected B. zoohelcum in oral cavities of therapy dogs in close contact with older people residing in nursing homes. Twenty-two bacterial isolates were identified as B. zoohelcum by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing. Our results showed that MALDI-TOF MS is an effective tool for rapid identification of rarely isolated, difficult-to-identify microorganisms, such as B. zoohelcum, derived from not only human clinical samples but also animal samples. To our knowledge, this is the first report on detection of B. zoohelcum from therapy dogs. We have provided information on dog-assisted therapy to improve the relationship between humans and animals in ageing societies, particularly for preventive healthcare of older people living in nursing care facilities.


Assuntos
Infecções por Flavobacteriaceae/veterinária , Flavobacteriaceae/isolamento & purificação , Boca/microbiologia , Animais , Doenças do Cão/microbiologia , Cães , Flavobacteriaceae/genética , Infecções por Flavobacteriaceae/transmissão , Instituição de Longa Permanência para Idosos , Humanos , Japão , RNA Ribossômico 16S/genética , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
8.
J Vet Med Sci ; 80(6): 985-988, 2018 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-29669948

RESUMO

Tick-borne encephalitis (TBE) and severe fever with thrombocytopenia syndrome (SFTS) are both tick-borne zoonotic diseases caused by TBE virus (TBEV) and SFTS phlebovirus (SFTSV). In 2016, a second domestic TBE case was reported in Hokkaido, Japan, after an absence of 23 years. We conducted IgG ELISA for TBEV and SFTSV on 314 deer (Cervus nippon yesoensis) serum samples collected from 3 places in Hokkaido. There were 7 seropositive samples for TBEV but none for SFTSV by ELISA. The specificity of the 7 positive samples was confirmed by neutralization tests against TBEV, and 5 sera showed 320 to 640 of 50% focus reduction endpoint titers. Our results provide information about the infectious status of TBEV in wild deer in Hokkaido, Japan.


Assuntos
Cervos/parasitologia , Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação , Encefalite Transmitida por Carrapatos/veterinária , Animais , Encefalite Transmitida por Carrapatos/epidemiologia , Feminino , Japão/epidemiologia , Masculino , Inquéritos e Questionários
9.
Southeast Asian J Trop Med Public Health ; 45(5): 1167-72, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25417520

RESUMO

A seroepidemiologic survey of Coxiella burnetii in cattle and chickens in Thailand was carried out using indirect fluorescent antibody test. Nine of the 130 serum samples from cattle were positive for antibodies against C. burnetii, with antibody titers ranging from 32 to 64. Only one of 113 serum samples from chickens was seropositive, with antibody titer of 16. No C. burnetii-specific DNA was detected using restriction fragment length polymorphism-nested PCR in spleens of cattle and chickens. However, coxiella DNA was detected in two of 102 engorged Rhipicephalus microplus ticks attached to dairy cattle. These results indicated that infestation of C. burnetii among cattle and chickens is considerably low in Thailand.


Assuntos
Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Doenças das Aves Domésticas/diagnóstico , Doenças das Aves Domésticas/epidemiologia , Febre Q/diagnóstico , Febre Q/epidemiologia , Criação de Animais Domésticos , Animais , Anticorpos Antibacterianos , Bovinos , Galinhas , Coxiella burnetii/imunologia , Coxiella burnetii/isolamento & purificação , Técnica Indireta de Fluorescência para Anticorpo , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Febre Q/veterinária , Rhipicephalus , Estudos Soroepidemiológicos , Tailândia/epidemiologia
10.
J Vet Med Sci ; 76(7): 1001-8, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24705506

RESUMO

There has been an accumulation of information on frequencies of insertion/deletion (indel) polymorphisms within the bovine prion protein gene (PRNP) and on the number of octapeptide repeats and single nucleotide polymorphisms (SNPs) in the coding region of bovine PRNP related to bovine spongiform encephalopathy (BSE) susceptibility. We investigated the frequencies of 23-bp indel polymorphism in the promoter region (23indel) and 12-bp indel polymorphism in intron 1 region (12indel), octapeptide repeat polymorphisms and SNPs in the bovine PRNP of cattle and water buffaloes in Vietnam, Indonesia and Thailand. The frequency of the deletion allele in the 23indel site was significantly low in cattle of Indonesia and Thailand and water buffaloes. The deletion allele frequency in the 12indel site was significantly low in all of the cattle and buffaloes categorized in each subgroup. In both indel sites, the deletion allele has been reported to be associated with susceptibility to classical BSE. In some Indonesian local cattle breeds, the frequency of the allele with 5 octapeptide repeats was significantly high despite the fact that the allele with 6 octapeptide repeats has been reported to be most frequent in many breeds of cattle. Four SNPs observed in Indonesian local cattle have not been reported for domestic cattle. This study provided information on PRNP of livestock in these Southeast Asian countries.


Assuntos
Búfalos/genética , Bovinos/genética , Variação Genética , Príons/genética , Animais , Indonésia , Príons/metabolismo , Tailândia , Vietnã
11.
Microbiol Immunol ; 58(3): 149-54, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24397564

RESUMO

To explore the prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) in veterinary medical practices, MRSA carriage was tested among 96 veterinarians (Vets), 70 veterinary technicians (VTs) and 292 dogs with which they had contact at 71 private veterinary clinics (VCs) in Hokkaido, Japan. MRSA isolates were obtained from 22 Vets [22.9%] and 7 VTs [10%]. The prevalence of MRSA among Vets was as high as that found in an academic veterinary hospital in our previous study. In contrast, only two blood donor dogs and one dog with liver disease (1.0%, 3/292) yielded MRSA. All MRSA-positive dogs were reared or treated in different VCs, in each of which at least one veterinary staff member carrying MRSA worked. Sequence types (ST) identified by multilocus sequence typing, spa types, and SCCmec types for canine MRSA isolates (ST5-spa t002-SCCmec II [from two dogs] or ST30-spa t021-SCCmec IV [from a dog]) were concordant with those from veterinary staff members in the same clinics as the MRSA-positive dogs, with which they had potentially had contact. Most MRSA isolates from veterinary staff were the same genotype (SCCmec type II and spa type t002) as a major hospital-acquired MRSA clone in Japan. The remaining MRSA was the same genotypes as domestic and foreign community-associated MRSA. Measures against MRSA infection should be provided in private VCs.


Assuntos
Técnicos em Manejo de Animais , Portador Sadio/microbiologia , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Infecções Estafilocócicas/microbiologia , Médicos Veterinários , Zoonoses/microbiologia , Adulto , Animais , Antibacterianos/farmacologia , Portador Sadio/epidemiologia , Portador Sadio/veterinária , Doenças do Cão/epidemiologia , Doenças do Cão/microbiologia , Cães , Feminino , Hospitais Veterinários , Humanos , Japão/epidemiologia , Masculino , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/genética , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/veterinária , Zoonoses/epidemiologia
12.
J Vet Med Sci ; 76(5): 685-92, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24476850

RESUMO

To determine the prevalence of indicator bacteria resistant to antimicrobials among poultry in three Southeast Asian countries (Vietnam, Indonesia and Thailand), we examined the antimicrobial susceptibilities of commensal bacteria isolated from chickens. In total, 125, 117 and 180 isolates of Escherichia coli, Enterococcus faecalis and Enterococcus faecium, respectively, were used to test for antimicrobial susceptibility. Bacterial resistance to antimicrobial treatment was most frequently observed with oxytetracycline with a prevalence of 73.6% (E. coli), 69.2% (E. faecalis) and 92.2% (E. faecium). Resistance to fluoroquinolones, which are critically important medicines, was also frequently observed in E. coli (48.8%), E. faecalis (17.9%) and E. faecium (82.8%). The prevalence of indicator bacteria resistant to most of the antimicrobials tested in these countries was higher than those for developed countries. The factors underlying antimicrobial resistance may include inappropriate and/or excessive use of antimicrobials. These results highlight the need for monitoring the emergence and prevalence of antimicrobial resistance in developing countries.


Assuntos
Galinhas , Resistência Microbiana a Medicamentos/genética , Monitoramento Epidemiológico/veterinária , Infecções por Bactérias Gram-Positivas/veterinária , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/microbiologia , Animais , Sudeste Asiático/epidemiologia , Enterococcus faecalis/genética , Enterococcus faecium/genética , Escherichia coli/genética , Fluoroquinolonas , Infecções por Bactérias Gram-Positivas/epidemiologia , Oxitetraciclina , Prevalência , Especificidade da Espécie
13.
J Vet Med Sci ; 76(12): 1627-9, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25649946

RESUMO

Veterinary staff carrying methicillin-resistant Staphylococcus aureus(MRSA) can be a source of MRSA infection in animals. To identify risk factors of MRSA carriage among veterinary staff, MRSA carriage and epidemiological information (sex, career, contact with MRSA-identified animal patients and others) were analyzed from 96 veterinarians and 70 veterinary technicians working at 71 private veterinary clinics in Japan. Univariate analysis determined sex (percentage of MRSA carriage, male (29.2%) vs. female (10%); P=0.002) and career (veterinarians (22.9%) vs. veterinary technicians (10%); P=0.030) as risk factors. Multivariable analysis revealed that sex was independently associated with MRSA carriage (adjusted odds ratio, 3.717; 95% confidence interval, 1.555-8.889; P=0.003). Therefore, male veterinary staff had a higher risk of MRSA carriage than female staff.


Assuntos
Técnicos em Manejo de Animais/estatística & dados numéricos , Portador Sadio/microbiologia , Staphylococcus aureus Resistente à Meticilina/genética , Infecções Estafilocócicas/epidemiologia , Médicos Veterinários/estatística & dados numéricos , Zoonoses/epidemiologia , Animais , Feminino , Humanos , Japão/epidemiologia , Masculino , Razão de Chances , Animais de Estimação , Fatores de Risco , Fatores Sexuais , Infecções Estafilocócicas/transmissão , Zoonoses/microbiologia
14.
Microbiol Immunol ; 57(10): 684-91, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23930694

RESUMO

To determine and compare the extent of contamination caused by antimicrobial-resistant lactic acid bacteria (LAB) in imported and domestic natural cheeses on the Japanese market, LAB were isolated using deMan, Rogosa and Sharpe (MRS) agar and MRS agar supplemented with six antimicrobials. From 38 imported and 24 Japanese cheeses, 409 LAB isolates were obtained and their antimicrobial resistance was tested. The percentage of LAB resistant to dihydrostreptomycin, erythromycin, and/or oxytetracycline isolated from imported cheeses (42.1%) was significantly higher than that of LAB resistant to dihydrostreptomycin or oxytetracycline from cheeses produced in Japan (16.7%; P=0.04). Antimicrobial resistance genes were detected in Enterococcus faecalis (tetL, tetM, and ermB; tetL and ermB; tetM) E. faecium (tetM), Lactococcus lactis (tetS), Lactobacillus (Lb.), casei/paracasei (tetM or tetW), and Lb. rhamnosus (ermB) isolated from seven imported cheeses. Moreover, these E. faecalis isolates were able to transfer antimicrobial resistance gene(s). Although antimicrobial resistance genes were not detected in any LAB isolates from Japanese cheeses, Lb. casei/paracasei and Lb. coryniformis isolates from a Japanese farm-made cheese were resistant to oxytetracycline (minimal inhibitory concentration [MIC], 32 µg/mL). Leuconostoc isolates from three Japanese farm-made cheeses were also resistant to dihydrostreptomycin (MIC, 32 to >512 µg/mL). In conclusion, the present study demonstrated contamination with antimicrobial-resistant LAB in imported and Japanese farm-made cheeses on the Japanese market, but not in Japanese commercial cheeses.


Assuntos
Antibacterianos/farmacologia , Queijo/microbiologia , Farmacorresistência Bacteriana , Lactobacillales/efeitos dos fármacos , Lactobacillales/isolamento & purificação , Técnicas Bacteriológicas , Sulfato de Di-Hidroestreptomicina/farmacologia , Eritromicina/farmacologia , Japão , Lactobacillales/genética , Testes de Sensibilidade Microbiana , Oxitetraciclina/farmacologia
15.
J Vet Med Sci ; 75(4): 407-14, 2013 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-23149545

RESUMO

Fluoroquinolone resistance is mainly caused by mutations in quinolone resistance-determining regions of DNA gyrase and topoisomerase IV in Escherichia coli. The AcrAB-TolC efflux pump contributes to resistance against fluoroquinolone and other antimicrobials. In this study, we investigated a high-level mechanism of fluoroquinolone resistance in E. coli that was isolated from human clinical samples and canine fecal samples. E. coli strains with high levels of fluoroquinolone resistance have been found to be frequently resistant to cephalosporins. Strains with high-level fluoroquinolone resistance exhibited lower intracellular enrofloxacin (ENR) concentrations, higher expression of AcrA, and a greater reduction in the fluoroquinolone minimum inhibitory concentration for treatment with an efflux pump inhibitor. The frequency of strains with enhanced ENR resistance selection and the survival rate of E. coli in the presence of ENR in vitro were correlated well with AcrA protein expression levels in the parental strains. These results suggest that AcrAB-TolC efflux pump over-expression is related to high-level fluoroquinolone resistance and the selection of strains with enhanced fluoroquinolone resistance.


Assuntos
Anti-Infecciosos/farmacologia , Doenças do Cão/microbiologia , Infecções por Escherichia coli/veterinária , Proteínas de Escherichia coli/metabolismo , Escherichia coli/efeitos dos fármacos , Fluoroquinolonas/farmacologia , Lipoproteínas/metabolismo , Proteínas de Membrana Transportadoras/metabolismo , Animais , DNA Bacteriano/química , DNA Bacteriano/genética , Doenças do Cão/tratamento farmacológico , Cães , Farmacorresistência Bacteriana/genética , Enrofloxacina , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Escherichia coli/metabolismo , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/microbiologia , Proteínas de Escherichia coli/genética , Humanos , Lipoproteínas/genética , Proteínas de Membrana Transportadoras/genética , Testes de Sensibilidade Microbiana/veterinária , Reação em Cadeia da Polimerase/veterinária
16.
J Vet Med Sci ; 74(1): 117-20, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21878745

RESUMO

Campylobacter was isolated from retail meat samples collected during the fiscal year 2009 in Japan. The higher percentages of contamination of chicken products were observed from June (39.3%) to November (83.3%). However, the highest number of human campylobacteriosis cases was reported in June in the Infectious Agents Surveillance Report. The chicken isolates with distinct clusters IVb and I, based on the restriction fragment length polymorphism of the flaA gene, were predominantly obtained during the periods between April and November 2009 and between February and March 2010, respectively. Extensive monitoring of Campylobacter contamination in chickens produced in various places is needed to analyse the seasonal variations between contamination of the meat products and the number of human cases with campylobacteriosis.


Assuntos
Infecções por Campylobacter/epidemiologia , Campylobacter/isolamento & purificação , Microbiologia de Alimentos , Carne/microbiologia , Estações do Ano , Animais , Campylobacter/efeitos dos fármacos , Bovinos , Galinhas , Farmacorresistência Bacteriana , Humanos , Japão/epidemiologia , Fígado/microbiologia , Fatores de Tempo
17.
Res Vet Sci ; 93(2): 574-80, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21975215

RESUMO

Factors associated with the carriage of antimicrobial-resistant Escherichia coli isolates were analysed among zoo animals. An association was observed between selection of amoxicillin as the first-line therapy and a significantly higher percentage of resistance to ampicillin (54.5%) from 11 animals treated with antimicrobials, compared with isolates from 32 untreated animals (9.4%). In addition, the percentage resistance to kanamycin (36.4%), gentamicin (27.3%), trimethoprim (27.3%) and tetracycline (63.6%) from 11 treated animals was significantly higher than those from 32 untreated animals (3.1%, 3.1%, 3.1% and 25%, respectively), although these antimicrobials were rarely used. All kanamycin-, gentamicin- and trimethoprim-resistant isolates and more than half of the tetracycline-resistant isolates from treated animals were also resistant to ampicillin. Co-resistance to other antimicrobials with ampicillin was suggested to contribute to an increasing of resistance towards antimicrobials that were rarely administered. The present investigation revealed an association of antimicrobial treatment with the spread of antimicrobial-resistant bacteria among zoo animals.


Assuntos
Animais de Zoológico , Antibacterianos/farmacologia , Farmacorresistência Bacteriana , Infecções por Escherichia coli/veterinária , Escherichia coli/efeitos dos fármacos , Animais , Escherichia coli/enzimologia , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Regulação Bacteriana da Expressão Gênica/fisiologia , Regulação Enzimológica da Expressão Gênica/fisiologia , Japão/epidemiologia , Filogenia , beta-Lactamases/genética , beta-Lactamases/metabolismo
18.
J Vet Med Sci ; 73(9): 1191-3, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21525710

RESUMO

To reveal the antimicrobial susceptibilities of Escherichia coli isolates from wild mice, 81 E. coli isolates were obtained from 109 voles (Clethrionomys spp.), 52 large Japanese field mice (Apodemus speciosus) and 19 small Japanese field mice (A. argenteus) captured in a forest of a natural park in Hokkaido, Japan. Seventy-eight of the 81 E. coli isolates were susceptible to all 10 antimicrobial agents tested. One E. coli isolate was resistant to ampicillin, dihydrostreptomycin, kanamycin, chloramphenicol and oxytetracycline. Two isolates were resistant to oxytetracycline. A low prevalence of antimicrobial resistance was maintained among wild mice that inhabited the forest.


Assuntos
Arvicolinae/microbiologia , Farmacorresistência Bacteriana , Infecções por Escherichia coli/veterinária , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Murinae/microbiologia , Animais , Animais Selvagens , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Japão/epidemiologia , Testes de Sensibilidade Microbiana , Árvores
19.
Appl Environ Microbiol ; 76(15): 5165-74, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20543040

RESUMO

Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of each in a veterinary college. MRSA and MRSP were isolated from nasal samples from veterinarians, staff members, and veterinary students affiliated with a veterinary hospital. Using stepwise logistic regression, we identified two factors associated with MRSA carriage: (i) contact with an identified animal MRSA case (odds ratio [OR], 6.9; 95% confidence interval [95% CI], 2.2 to 21.6) and (ii) being an employee (OR, 6.2; 95% CI, 2.0 to 19.4). The majority of MRSA isolates obtained from individuals affiliated with the veterinary hospital and dog patients harbored spa type t002 and a type II staphylococcal cassette chromosome mec (SCCmec), similar to the hospital-acquired MRSA isolates in Japan. MRSA isolates harboring spa type t008 and a type IV SCCmec were obtained from one veterinarian on three different sampling occasions and also from dog patients. MRSA carriers can also be a source of MRSA infection in animals. The majority of MRSP isolates (85.2%) carried hybrid SCCmec type II-III, and almost all the remaining MRSP isolates (11.1%) carried SCCmec type V. MRSA and MRSP were also isolated from environmental samples collected from the veterinary hospital (5.1% and 6.4%, respectively). The application of certain disinfection procedures is important for the prevention of nosocomial infection, and MRSA and MRSP infection control strategies should be adopted in veterinary medical practice.


Assuntos
Portador Sadio/microbiologia , Portador Sadio/veterinária , Resistência a Meticilina , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/veterinária , Staphylococcus/classificação , Staphylococcus/isolamento & purificação , Animais , Técnicas de Tipagem Bacteriana , Impressões Digitais de DNA , DNA Bacteriano/genética , Transmissão de Doença Infecciosa/prevenção & controle , Cães , Microbiologia Ambiental , Hospitais Veterinários , Humanos , Controle de Infecções/métodos , Japão , Epidemiologia Molecular , Nariz/microbiologia , Staphylococcus/efeitos dos fármacos , Staphylococcus/genética , Estudantes , Médicos Veterinários
20.
J Vet Med Sci ; 68(9): 1007-8, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17019076

RESUMO

The indirect fluorescent antibody test (IFAT) revealed seropositivity to Coxiella burnetii in aged non-laying chickens in poultry farms in a prefecture in the central part of Japan. Seropositivity was 7%, and antibody titers ranged from 16 to 64. No DNA fragment specific for C. burnetii was detected in the chickens by nested-PCR. The prevalence of C. burnetii infection in a prefecture of Japan in which poultry farming prospers was 7%.


Assuntos
Agricultura , Galinhas/microbiologia , Coxiella burnetii/isolamento & purificação , DNA Bacteriano/análise , Doenças das Aves Domésticas/microbiologia , Animais , Anticorpos Antibacterianos/sangue , Galinhas/sangue , Coxiella burnetii/genética , DNA Bacteriano/genética , Feminino , Imunoglobulina G/sangue , Japão , Doenças das Aves Domésticas/sangue , Doenças das Aves Domésticas/diagnóstico , Estudos Soroepidemiológicos
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