Surface Coating of Nickel-Titanium (Ni-Ti) Pediatric Rotary File Using Graphene Oxide: A Scanning Electron Microscopy Analysis.

BACKGROUND: The effectiveness of endodontic files relies significantly on the characteristics of the outermost layer, which can be greatly improved through suitable surface treatments and appropriate coatings. Graphene-based materials (GBMs) have been utilized to fabricate nanocomposite coatings aimed at improving surface characteristics and mechanical behavior, including resilience, sustainability, oxidation resistance, solidity, and traction. AIM: This research aims to study the surface topography of a nickel-titanium (Ni-Ti) pediatric rotary file coated with graphene oxide (GO) using a scanning electron microscope (SEM). METHODS: The study utilized Ni-Ti pediatric rotary instruments that were 16 mm long and had the same ISO tip size of #25. The Ni-Ti pediatric rotary files had a titanium oxide coating that needed to be removed for the application of the GO coating. The GO coating was applied to the files using an electrophoretic deposition (EPD) procedure. Data were gathered to evaluate the surface topography and structural profiles of the GO-coated endodontic files through SEM analysis. RESULTS: SEM imaging showed that the GO coatings consisted of numerous layers of GO sheets, which were uniformly and thoroughly applied to the endodontic instrument. A substantial portion of the GO layers aligned with neighboring layers along the edges, creating a continuous structure. CONCLUSION: GO coatings were effectively applied to Ni-Ti endodontic instruments using EPD. The deposition of the GO coating is consistent throughout the surface of the Ni-Ti rotary instrument.

Role of Oral Veillonella Species in Predicting Surgical Site Infections After Maxillofacial Trauma: A Prospective Observational Study.

INTRODUCTION: There are comparatively fewer surgical site infections after craniofacial trauma than after trauma to the extremities, and the etiology is complex. Gram-negative facultative anaerobic bacteria Veillonella is a common commensal in the oral cavity and has been linked to osteomyelitis and surgical site infections in prosthetic joint infections. They serve as early biological indicators. AIMS/OBJECTIVES: This study aims to assess the presence of Veillonella in patients presenting with maxillofacial trauma, to document the difference in colony count in patients requiring surgical intervention at different time intervals as against patients with surgical site infections, and to provide better hospital care and management so as to improve the standard of care with an attempt to prevent the possibility of postoperative surgical site infections. METHODOLOGY: In this study, individuals with trauma/fractures of the maxillofacial region requiring surgical intervention at varied time spans, early, intermediate, and late, were included. After obtaining informed consent, the examination was done; the fracture type and site were noted, and a swab was taken on the day of admission, on the day of surgery, and on the day of discharge and given for microbiological evaluation. Findings were recorded. RESULTS: The primary and secondary objectives of the study were established. The mean colony count in colony-forming units/milliliter for patients undergoing early surgical intervention, on the day of admission, was 2.01E+0.6. On the day of discharge, the mean colony count was 1.51E+0.6. In contrast, for patients with surgical site infection, on the day of admission, the mean was 6.5E+0.7, and on the day of discharge, the mean colony count reduced to 4.01E+0.6. The time-colony-forming unit graph showed a difference in the colony count of Veillonella in patients operated at different time intervals as against patients with surgical site infection and modified relation with a number of other oral commensals. The colony count in patients with osteomyelitis was found and compared. CONCLUSION: There is a change in the colony count of Veillonella species and its relation to their commensals when intervened at different time intervals. Our study indicates that the estimation of Veillonella species and the colony count could aid in determining the possibility of a surgical site infection. This study also stresses on the appropriate reporting of maxillofacial trauma in cases of a poly-trauma for appropriate management.

Evaluating the Prevalence of Nasolacrimal Canal Injury in Midfacial Fractures: A Retrospective CT-Based Study.

INTRODUCTION: Midface injuries, which are most common, can result in affectations to adjacent structures, including the nasolacrimal apparatus (NLA), which consists of the lacrimal sac, canaliculi, and nasolacrimal duct. OBJECTIVES: The objectives of this study were to visualise the radiographic patency of the nasolacrimal canal in a computed tomography (CT) scan and assess the type of injury. METHODOLOGY: This was a retrospective study wherein 322 CT scans of facial bones were analysed of patients who presented with midface fractures to the Department of Oral and Maxillofacial Surgery. The bony nasolacrimal canal was visualised on axial and coronal sections and confirmed on sagittal sections. The collapse of the bony nasolacrimal canal was measured using the advanced tools setting in the RadiAnt DICOM Viewer (Medixant, Poznan, Poland). The values were tabulated and statistically analysed. RESULTS: The incidence of NLA involvement in midface fractures was 37.6% (121 out of 322 fractures). The maximal involvement was seen in zygomaticomaxillary complex (ZMC) fractures. The visualisation of the fractures in the CT scan revealed that avulsion of the fossa was seen in 2.5% (eight out of 322 fractures), communication of the fossa or canal in 2.8% (nine out of 322 fractures), and linear fracture of the canal in 32.0% (103 out of 322 fractures). When measured in axial section, the Le Fort III fractures presented with a median of 2.15 mm, naso-orbito-ethmoid (NOE) fractures with a median of 0.90 mm, and fronto-naso-orbito-ethmoid (FNOE) fractures with a median of 1.15 mm. In the coronal section, the type of injuries that showed avulsion of the fossa had a median of 9.00 mm, communication of the fossa or canal showed 6.52 mm and linear fracture of the canal showed a median of 7.00 mm. CONCLUSION: Many a time, the NLA is often neglected during a routine radiographic assessment of a CT scan in patients presenting with maxillofacial injuries. These injuries may not be clinically evident during examination. This might lead to postoperative clinical presentations in a patient. This study shows the various types of injuries to the NLA and its appearance on a CT scan. It also explains the requirement of soft tissue management and the clinical co-relationship of these injuries.

Bibliometric Analysis of Dental Caries Detection.

The main purpose of this bibliometric analysis is to conduct a quantitative and qualitative analysis of the publications on dental caries. Research productivity can be measured with the use of bibliometric analysis. In this study, we conducted a bibliometric analysis using the Scopus database to identify dental caries research trends and patterns over the years. The search yielded 1630 scientific articles. The data was analysed using bibliometric indicators such as the h-index, the total number of citations and the number of publications. An analysis of the data highlighted that the United States of America (USA) and Brazil have the highest number of single-country publications. Top authors were listed based on the h-index and the total number of citations. Top cited countries, institutions etc. were also analysed using this bibliometric study. This bibliometric evaluation provides a wide area of literature carried out to date. The existing knowledge can be used to direct future research.

Biocompatibility of Chitosan Nanoparticle in Root Canal Sealant with Vero Cell Line.

Root canal sealants placed in primary tooth are unique in various characteristics. Chitosan nanoparticles have gained an important milestone in research area due to its biodegradable and its bioavailability nature. Chitosan nanoparticles synthesized by ionic gelation method and studied for its physical characteristics when added with root canal sealant provides a promising result. In this study, biocompatibility nature of Chitosan nanoparticles with Vero cell line was being investigated. Several solution parameters of Chitosan nanoparticles and root canal sealants were investigated to optimize diameter of nanoparticles after being characterized to XRD, SEM, and FTIR. The Chitosan nanoparticles were found to be biocompatible to fibroblasts and on a dose-dependent manner, these can be used in combination with the root canal sealant in primary teeth. How to cite this article: Ramar K, N V. Biocompatibility of Chitosan Nanoparticle in Root Canal Sealant with Vero Cell Line. Int J Clin Pediatr Dent 2022;15(S-1):S57-S62.

Evaluation of cytotoxicity levels of poly vinyl ether silicone, polyether, and poly vinyl siloxane impression materials: An in vitro study.

AIM: To assess the cytotoxicity level of newly introduced poly vinyl ether silicone (PVES) compared to poly vinyl siloxane (PVS) and polyether (PE) elastomeric impression materials. SETTINGS AND DESIGN: Comparative -Invitro study design. MATERIALS AND METHODS: Mouse cell line NIH/3T3 was grown in Dulbecco's modified Eagle's medium. Samples of three elastomers were dissolved in dimethyl sulfoxide and were tested at various concentrations. Twenty-four well plates with NIH/3T3 cells with different concentrations of elastomeric solutions were incubated at 37°C. 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay was performed on day 1, 3, and 7, with a time interval of 15 min, 30 min, 60 min, and 24th h to estimate the cytotoxicity for all three elastomers. STATISTICAL ANALYSIS USED: Kruskal-Wallis ANOVA test and the period effect within the subjects, repeated-measure ANOVA was done using the Greenhouse-Geisser correction method. RESULTS: The mean cell viability (survival rate) of NIH 3T3 cells at the concentrations tested was measured. A repeated-measure Kruskal-Wallis ANOVA determined the mean survival concentration on day 1, 3, and 7. PVES showed significant decrease in the survival rate on day 1 than PVS and PE, while PVS and PE had significant decrease in the survival rates of cells on day 3 and 7 which were statistically significant (P < 0.001). CONCLUSION: PVES shows early cytotoxic signs as compared to PVS and PE, and cell viability for PVS was the highest among all. When making impression with PVES and PE, it is always better to evaluate the impression and gingival sulcus carefully with magnification to prevent adverse reaction, if any material is left inadvertently for longer period of time.