RESUMO
Isothiocyanates (ITCs) have gained increasing attention since they have been attributed the merits for the potential beneficial effects of cruciferous vegetable dietary consumption on cancer. The aim of the present study was to determine the cytotoxic effects of 3-butenyl ITC (3-BI) on prostate cancer (PC) cells under in vitro conditions. Two androgen-insensitive human PC cell lines, PC-3 and DU145, were assayed. Cells were cultured in the presence of increasing concentrations of 3-BI (5, 10, 30 and 50 µM) in the absence or presence of the chemotherapeutic drug docetaxel (DOCE) (1 and 2 nM). The cytotoxic effects of these compounds were analyzed using the trypan blue exclusion assay at 24, 48 and 72 h. Apoptosis and migration assays were also performed. The results showed that 3-BI induced a dose-dependent cytotoxic effect on PC-3 cells at 24, 48 and 72 h. These effects were significantly higher than those found with DOCE at 72 h of culture. Moreover, 3-BI also potentiated the effects of DOCE in a dose-dependent manner. Additionally, 3-BI showed inhibition of the migration of PC-3 cells. Nevertheless, 3-BI was not effective in the DU145 PC cell line. These results show a promising role for the 3-BI compound as a co-adjuvant agent in DOCE-based therapy in certain types of PC.
Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Docetaxel/farmacologia , Isotiocianatos/farmacologia , Neoplasias da Próstata/tratamento farmacológico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Docetaxel/uso terapêutico , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Humanos , Isotiocianatos/uso terapêutico , MasculinoRESUMO
Experiments were conducted to evaluate the effects of amphetamine (0. 4 mg/kg) on the development of autochthonous tumors induced by the Moloney sarcoma virus (MSV) in Balb/c female mice. Enhancement of MSV-induced tumor incidence and tumor growth was observed, together with a delay in the usual prompt regression of the tumors, when mice were daily injected with amphetamine for 3 days after MSV-inoculation. However, no effects of amphetamine on tumor development were observed when it was administered during the 3 days before tumor inoculation.
Assuntos
Adrenérgicos/farmacologia , Anfetamina/farmacologia , Vírus do Sarcoma Murino de Moloney , Infecções por Retroviridae/patologia , Sarcoma Experimental/patologia , Infecções Tumorais por Vírus/patologia , Animais , Feminino , Imunidade Celular/efeitos dos fármacos , Injeções Subcutâneas , Camundongos , Camundongos Endogâmicos BALB C , Pré-Medicação , Infecções por Retroviridae/tratamento farmacológico , Sarcoma Experimental/tratamento farmacológico , Infecções Tumorais por Vírus/tratamento farmacológicoRESUMO
Mice injected with amphetamine showed a dose-related suppression of the natural killer cell activity. The capacity of T-cells to generate cytotoxic T-lymphocytes (CTL) in mixed lymphocyte cultures and in vivo was also assayed and amphetamine was found to inhibit CTL responses.
Assuntos
Anfetamina/farmacologia , Imunidade Celular/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Células Matadoras Naturais/efeitos dos fármacos , Células Matadoras Naturais/imunologia , Teste de Cultura Mista de Linfócitos , Masculino , Camundongos , Camundongos Endogâmicos , Baço/citologia , Baço/imunologia , Linfócitos T/efeitos dos fármacos , Linfócitos T/imunologia , Linfócitos T Citotóxicos/efeitos dos fármacos , Linfócitos T Citotóxicos/imunologiaRESUMO
Experiments were conducted to evaluate the effects of alprazolam (1 mg/kg i.p.) on the development of autochthonous tumors induced by the Moloney sarcoma virus (MSV) in BALB/c female mice subjected to stress. Enhancement of MSV-induced tumor incidence and growth was observed, together with a delay in the usual prompt regression of the tumors, when programmed white sound anxiety-stress was administered immediately following MSV i.m. inoculation. However, a depressant effect on tumor size and incidence was observed when stress was administered before virus inoculation. Treatment with alprazolam was found to reverse partially the adverse effects of postinoculation stress, and also to inhibit the beneficial effects of the preinoculation administration of stress on tumor development. Pretreatment with Ro 15-1788 (10 mg/kg s.c.), a central benzodiazepine antagonist, resulted in a suppression on both effects of alprazolam in stressed mice.