RESUMO
Cytostatic activity of baicalin, baicalein, and neogalenical drug Chlorophyllipt was studied in vitro on HeLa-v cells. Standard samples of Eucalimin, baicalin, and baicalein, as well as Chlorophyllipt and paclitaxel (reference drug Taxacad) were used. The cell deaths were determined by MTT assay in a Multiskan FC microplate reader with incubator. The effective inhibition concentration (IC50) of the tested substances were: paclitaxel (4.0±0.4 µM)-baicalein (10.5±1.1 µM)-baicalin (16.5±1.7 µM)-sum of euglobals in Chlorophyllipt (24.1±2.5 µM). Chlorophyllipt was found to exhibit cytostatic activity. Cytostatic activity of baicalein, baicalin, and Chlorophyllipt was lower than cytostatic activity of the reference drug by 2.6, 4.1, and 6 times, respectively. The prospects of further evaluation of the synergetic effect of baicalin, baicalein, and chlorophyllipt used in combinations with different cytostatic agents for finding the most effective combination have been shown.
Assuntos
Citostáticos , Flavanonas , Humanos , Citostáticos/farmacologia , Flavonoides/farmacologia , Flavanonas/farmacologia , Flavanonas/metabolismo , Células HeLa , PaclitaxelRESUMO
We studied the expression of transcription factors RUNX2 and Osterix after addition of a concentrate of osteogenic-conditioned medium to the culture medium for osteogenic differentiation of mesenchymal stem cells (MSC). The obtained concentrate of osteogenic-conditioned medium containing a complex of bioactive substances with a molecular weight >10 kDa provided MSC differentiation into osteoblasts, which was confirmed by high level of expression of transcription factors RUNX2 and Osterix in comparison with the negative control. The highest expression of transcription factor Osterix was revealed on day 14 of MSC culturing in the presence of osteogenic supplement StemPro (positive control) and the studied concentrate of osteogenic-conditioned medium.
Assuntos
Subunidade alfa 1 de Fator de Ligação ao Core/genética , Meios de Cultivo Condicionados/farmacologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Osteoblastos/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Fatores de Transcrição/genética , Animais , Ácido Ascórbico/farmacologia , Biomarcadores/metabolismo , Células da Medula Óssea/citologia , Células da Medula Óssea/efeitos dos fármacos , Células da Medula Óssea/metabolismo , Diferenciação Celular/efeitos dos fármacos , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Meios de Cultivo Condicionados/química , Dexametasona/farmacologia , Expressão Gênica , Glicerofosfatos/farmacologia , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo , Osteoblastos/citologia , Osteoblastos/metabolismo , Osteogênese/genética , Cultura Primária de Células , Ratos , Ratos Wistar , Fatores de Transcrição/metabolismoRESUMO
We studied the influence of the characteristics of oxide porous coatings on osteogenesis and integration of new bone tissue and titanium implant surface in the ectopic osteogenesis test. Implants with coating with pore size 2-20 µ exhibit better osteogenic and osteoconductive characteristics than implants with homogenous surface and smooth relief (pore size 0.5-5.0 µ). Physical characteristics of the surface of the material along with chemical composition of the coating are essential for the formation of bone tissue and its integration with the implant.
Assuntos
Células da Medula Óssea/efeitos dos fármacos , Interface Osso-Implante/anatomia & histologia , Compostos de Cálcio/farmacologia , Materiais Revestidos Biocompatíveis/farmacologia , Osteoblastos/efeitos dos fármacos , Óxidos/farmacologia , Titânio/farmacologia , Animais , Células da Medula Óssea/citologia , Células da Medula Óssea/fisiologia , Interface Osso-Implante/fisiologia , Diferenciação Celular , Coristoma/diagnóstico por imagem , Implantes Dentários , Masculino , Osteoblastos/citologia , Osteoblastos/fisiologia , Osteogênese/efeitos dos fármacos , Porosidade , Cultura Primária de Células , Ratos , Ratos Wistar , Propriedades de Superfície , Titânio/químicaRESUMO
The osteoinductive characteristics of hydroxylapatite-based coating with different physicochemical characteristics were studied. The formation of the bone tissue on the studied types of the implant coating differed by the pattern and rate of formation of structural elements of the bone. Clear-cut phase-wise osteogenesis was seen on combined calcium phosphate coating: intense formation of the fibrous interstitial substance (due to cellular structure of hydroxylapatite) and slow maturing of cell elements. Bilayer composite coating provided (during the same period) the formation of more mature connective tissue. This phenomenon can be explained by better bioavailability of the coating material. The properties of combined calcium phosphate coating can be evaluated as mainly osteoconductive, while those of bilayer composite coating as osteoinductive.
Assuntos
Materiais Revestidos Biocompatíveis , Durapatita , Osteogênese/fisiologia , Animais , Osso e Ossos/citologia , Osso e Ossos/patologia , Osso e Ossos/fisiologia , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/metabolismo , Durapatita/química , Durapatita/metabolismo , Implantes Experimentais , Teste de Materiais , Distribuição Aleatória , Ratos , Propriedades de Superfície , Titânio/químicaRESUMO
The possibility of using the atomic-force microscopy for the evaluation of the geometrical characteristics of blood cells has been shown. It has been found by comparing the hemocyte morphometric indices obtained at different modes of scanning that noncontact and semicontact methods are adequate for the estimation of the size and geometry of biological objects. The scanning of cells in the semicontact mode leads to the irreversible deformation of samples.
Assuntos
Eritrócitos/citologia , Linfócitos/citologia , Animais , Tamanho Celular , Galinhas , Microscopia de Força Atômica , RatosRESUMO
Based on the method of hypoosmotic loads, the value of membrane reserve and its use by blood cells of reptiles and mammals have been studied. It has been shown that lymphocytes of the both animal species have the highest reserve of plasmalemma, while frog heterophils - the lowest one. A significantly lower part of the membrane reserve participates in formation of phagosomes by mammalian neutrophils as compared with amphibian erythrocytes.
Assuntos
Membrana Celular/fisiologia , Eritrócitos/citologia , Linfócitos/citologia , Neutrófilos/citologia , Fagocitose/fisiologia , Animais , Tamanho Celular , Células Cultivadas , Meios de Cultura , Membrana Eritrocítica/fisiologia , Eritrócitos/fisiologia , Linfócitos/fisiologia , Neutrófilos/fisiologia , Concentração Osmolar , Rana ridibunda , Ratos , Especificidade da EspécieRESUMO
Iron-deficiency anemia in pregnant females negatively affects pregnancy, delivery, neonatal infants. Anemia-caused placental changes found during pregnancy are characterized by imbalance between the processes of adaptation and dysadaptation in response to the developed hypoxia in the pregnant-placenta-fetus system and placental tissue ischemia. When iron-deficiency anemia had developed before pregnancy, placental morphological changes show the signs of chronic placental insufficiency. The developed therapy for anaemia with Ferro-Folgama corrects all the elements of the pregnant-placenta-fetus system.