Enriched advanced platelet-rich fibrin plus gold nanoparticles against Enterococcus faecalis for its potential use in revascularization for necrotic immature permanent teeth.

Background: Regenerative endodontic procedures allow reinforcement of root canal wall and continuation of root development, opening new therapeutic possibilities. The root canal system of infected teeth is colonized by a variety of microorganisms, which hinder the regenerative process, leading to treatment failure if not adequately addressed, thereby requiring careful attention to microbial control. Aim and Objective: The aim of the study was to assess the antimicrobial activity of advanced platelet-rich fibrin (A-PRF) and gold nanoparticles (AuNps) against Enterococcus faecalis. Materials and Methods: Intravenous blood (5-6 ml) was drawn from four healthy individuals, and A-PRF was prepared through centrifugation at 1500 revolutions per minute (rpm) for 14 min. A-PRF was doped with 3 µl of AuNps and centrifuged at 1000 rpm for 1 min. Antimicrobial activity was assessed using disk diffusion; inhibition zones were measured. For minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), A-PRF + AuNps were added to the microbial broth at varying concentrations to determine growth inhibition and microbial death. Results: Disk diffusion assays revealed significant antibacterial effects against E. faecalis. Norfloxacin displayed the highest mean zone of inhibition (20.33 ± 1.53 mm), followed by the Test group (A-PRF + AuNPs) (19.33 ± 0.58 mm). Multiple comparisons indicated significant differences (P < 0.001). MIC of A-PRF + AuNPs against E. faecalis was 0.031 mg/ml, with MBC at 0.015 mg/ml. Conclusion: The addition of AuNPs to A-PRF offers the potential for sustained growth factor release while maintaining the sterility of the canal, leading to successful revitalization and regeneration. The combined use of A-PRF + AuNps shows promise for enhancing revascularization in necrotic immature permanent teeth.

Evaluation of the effect of remineralization with strontium-doped nanohydroxyapatite with noncollagenous protein analog: Chitosan on the shear bond strength of resin composite to dentin - An in vitro study.

Context: The resin-dentin interface is less durable, which reduces the longevity of tooth-colored restorations. To encounter this shortcoming, the use of nanotechnology to mimic biomineralization proves beneficial. Aims: This study was conducted to evaluate the effect of remineralization with strontium-doped nanohydroxyapatite (Sr-nHAp) with chitosan on shear bond strength of resin composite to dentin. Materials and Methods: Sixty five extracted human premolars were divided into five groups (n = 13) based on remineralization protocol as: Group A - 20% (Sr-nHAp) with chitosan, Group B - 10% (Sr-nHAp) with chitosan, Group C - 20% (Sr-nHAp) with simulated body fluid, Group D - 10% (Sr-nHAp) with simulated body fluid, and Group E - control. Following bonding, resin composite of specified dimension was built and was subjected to shear bond strength test after 24 h and 1 week using Universal Testing Machine, and mode of failure was assessed. ANOVA and paired sample t-test were used for analyzing the data, and the level of significance was set at 5%. Results: The highest value of shear bond strength was obtained from Group A after aging for a week, and there is a significant increase in the value of all the groups as compared to the control group after 1 week of storage than 24 h. Conclusions: Remineralization with Sr-nHAp and chitosan has positively improved the bond strength of resin to dentin at the end of 1 week.

Effect of Staining and Bleaching on Color stability and Surface Roughness of Three Resin Composites: An in vitro study.

AIM: This study evaluated the effect of staining and subsequent bleaching on the color stability and surface roughness of three resin composites. MATERIALS AND METHODS: One hundred and eight customized plastic disks from a plastic mold with an outer diameter of 10 mm and thickness of 1.02 mm were fabricated. Samples were divided into three groups of 36 specimens each - Group I (Estelite® α - Supranano), Group II (Filtek™ Z250 XT - Nanohybrid), and Group III (Filtek™ P90-Silorane). Subsequently, baseline surface color and roughness values were tabulated using spectrophotometer and profilometer, respectively. The specimens were then subjected to staining with coffee and turmeric for a period of 3 h/day for 40 days and following this, bleaching of the samples was done using a bleaching agent carbamide peroxide gel 15% for 8 h/day for 14 consecutive days. RESULTS: Estelite® α performed better by maintaining color stability with minimal roughness compared to Filtek™ P90 and Filtek™Z250 XT. Turmeric had a greater potential to stain composites compared to coffee in all the groups.

Effect of Desensitization Using Bioactive Glass, Hydroxyapatite, and Diode Laser on the Shear Bond Strength of Resin Composites Measured at Different Time Intervals: An In vitro Study.

BACKGROUND: Dentin desensitizers may change the properties of smear layer and have adverse effects on the bonding performance of adhesive systems. AIM: The aim of this study was to compare the effect of bioactive glass (BG), hydroxyapatite, and diode laser desensitization on shear bond strength of resin composites to dentin at different time intervals. MATERIALS AND METHODS: Seventy-two caries-free maxillary premolars were selected. Buccal surfaces were flattened to expose dentin. Teeth were divided into four groups (Groups 1, 2, 3, and 4) according to treatment modality (control with no pretreatment, Sensodyne Repair and Protect, Teethmate Desensitizer, diode laser). Bonding was performed using self-etch adhesive followed by composite buildup. Universal testing machine was used to determine shear bond strengths immediately after bonding, after 3 months, and 5 months storage in artificial saliva. RESULTS: Pretreatment with BG and hydroxyapatite desensitizers increased, whereas diode laser decreased mean shear bond strength of composite to dentin as compared to control group. No statistical significant difference in shear bond strength values was seen in groups after storage. CONCLUSION: Desensitizing toothpastes incorporating remineralizing agents not only occluded open dentinal tubules but also increased shear bond strength of composite to dentin.

The effect of 2% chlorhexidine on the bond strength of two different obturating materials.

The bond strength of ActiV GP and gutta-percha/AH Plus after using 2% CHX as final rinse was evaluated in this study. According to the final irrigation regimen forty roots were divided into two groups (n = 20) randomly: Group 1-5 ml of 17% EDTA; Group 2-5 ml of 17% EDTA followed by 5 ml of 2% chlorhexidine gluconate (CHX). Based on the obturation system the two groups were further subdivided into two subgroups (n = 10). The roots were horizontally sectioned from the coronal and midthird sections for measuring the push-out bond strength. CHX/AH Plus (2.795 ± 0.42 MPa) and EDTA/ActiV GP (1.489 ± 0.117 MPa) recorded the highest and lowest mean bond strength values respectively. It was concluded that when 2% CHX was used as a final irrigation rinse the bond strength of both gutta-percha/AH Plus and ActiV GP obturation system improved significantly.