Reproductive Risk Factors Associated with Female Infertility in Sonepat District of Haryana: A Community Based Cross-Sectional Study.

Background: Infertility is a disease of the male or female reproductive system defined by the failure to achieve a pregnancy after 12 months or more of regular unprotected sexual intercourse. Aims: The aim of this study was to estimate the prevalence of infertility and its association with reproductive risk factors amongst women of reproductive age group (18-49 years) in district Sonepat, Haryana. Settings and Design: This observational community-based cross-sectional study was conducted amongst 444 reproductive age group (18-49 years) women residing in district Sonepat, from August 2021 to May 2022. Materials and Methods: A simple random sampling technique was used to select the study subjects. After taking written informed consent, all the participants were interviewed using a pre-designed, pre-tested semi-structured questionnaire for desired information. Statistical Analysis Used: Mean and standard deviation (SD) were calculated for quantitative data. Percentages and proportions were calculated for qualitative data. Results: Out of 444 study population, majority of women were fertile (88.7%), while 6.3% of women were secondary infertile and 5% of women were primary infertile. Most women were aged between 30 and 39 years. The difference of occurrence of infertility in relation to genital discharge (P = 0.049), genital ulcer/sores (P ≤ 0.001), groin swelling (P ≤ 0.001), warts (P = 0.015), menstrual cycle duration (P ≤ 0.001) and menstrual flow amount (P = 0.048) was statistically significant. The mean age of menarche for the female with infertility was 14.34 years (standard deviation = 1.40). Conclusion: Almost all of the symptoms of sexually transmitted infection/reproductive tract infection were high amongst infertile females. Awareness generation about the preventable risk factors and provision of infertility care services at primary healthcare facilities will be of use in addressing infertility in Sonepat.

Development of marker-free insect resistant transgenic okra (Abelmoschus esculentus L. Moench) expressing the cry1Ac gene and identification of vector backbone-free events.

Agrobacterium-mediated co-transformation method was used to generate marker-free insect resistant transgenic okra plants expressing the cry1Ac gene. The cry1Ac gene was borne on the T-DNA of one plasmid while nptII and uidA (GUS) marker genes were present on the T-DNA of a second plasmid. Putative transgenic plants were screened by histochemical GUS assay for expression of -glucuronidase and 32 transgenic events were positive for GUS in which 21 transgenic events were positive in ELISA for the presence of Cry1Ac protein. Out of 21 Cry1Ac positive T0 events, three events displayed Mendelian inheritance of the transgenes in (9:3:3:1 ratio) T1 generation for Cry1Ac and GUS. Selected events were chosen for further genetic and molecular analysis. The cry1Ac and marker genes were found to segregate independently, of each other in 10 events in T1 generation out of 11 Cry1Ac gene inheriting events analysed indicating that the two T-DNAs insertions were genetically unlinked and identification of marker-free plants were possible in these 10 events. The marker-free nature and vector backbone-free Bt events (clean T-DNA insertions carrying cry1Ac gene) were confirmed by Southern analysis using suitable probes. The plants from selected transgenic events were rigorously screened in whole plant insect bioassays using the larvae of shoot and fruit borer, Earias vittella, an important pest of okra. Insect bioassays indicated 100% larval mortality without any infestation in five of the transgenic events and two events showed 5 to 10 percent infestation establishing the insect resistant nature of the transgenic plants. Finally the events inheriting transgenes in Mendelian fashion were characterized further and marker-free and vector backbone-free events were identified showing complete protection from the target pest Earias vittella in whole-plant insect bioassays. Quantification of Cry1Ac protein levels in the plant parts of selected events (lines) was consistent with the results of bioassays. Further, two lines identified in this study met the criteria for inclusion in commercial breeding programs.

Efficient genetic transformation of okra (Abelmoschus esculentus (L.) Moench) and generation of insect-resistant transgenic plants expressing the cry1Ac gene.

KEY MESSAGE: Agrobacterium -mediated transformation system for okra using embryos was devised and the transgenic Bt plants showed resistance to the target pest, okra shoot, and fruit borer ( Earias vittella ). Okra is an important vegetable crop and progress in genetic improvement via genetic transformation has been impeded by its recalcitrant nature. In this paper, we describe a procedure using embryo explants for Agrobacterium-mediated transformation and tissue culture-based plant regeneration for efficient genetic transformation of okra. Twenty-one transgenic okra lines expressing the Bacillus thuringiensis gene cry1Ac were generated from five transformation experiments. Molecular analysis (PCR and Southern) confirmed the presence of the transgene and double-antibody sandwich ELISA analysis revealed Cry1Ac protein expression in the transgenic plants. All 21 transgenic plants were phenotypically normal and fertile. T1 generation plants from these lines were used in segregation analysis of the transgene. Ten transgenic lines were selected randomly for Southern hybridization and the results confirmed the presence of transgene integration into the genome. Normal Mendelian inheritance (3:1) of cry1Ac gene was observed in 12 lines out of the 21 T0 lines. We selected 11 transgenic lines segregating in a 3:1 ratio for the presence of one transgene for insect bioassays using larvae of fruit and shoot borer (Earias vittella). Fruit from seven transgenic lines caused 100 % larval mortality. We demonstrate an efficient transformation system for okra which will accelerate the development of transgenic okra with novel agronomically useful traits.

Reliability and validity of judgments of sound quality in elderly hearing aid wearers.

OBJECTIVE: The aim of the present study was to evaluate a particular sound-quality rating procedure, referred to here as the Judgments of Sound Quality (JSQ) test, for potential use as an outcome measure with elderly hearing aid wearers. The validity and reliability of the JSQ as an outcome measure were determined for unaided and aided listening conditions. DESIGN: A repeated-measures design was employed with two primary independent variables, each with two values: 1) aided condition (aided versus unaided listening); and 2) trial (test versus retest). Sixteen elderly, hearing-impaired hearing aid users participated in the study. The participants provided ratings on eight dimensions of sound quality (softness, brightness, clarity, fullness, nearness, loudness, spaciousness, and total impression) under four stimulus conditions (speech at 65 dB SPL with a +8 dB signal-to-noise ratio, speech at 65 dB SPL in quiet, music at 90 dB SPL, and music at 75 dB SPL). Test and retest ratings were obtained in both unaided and aided conditions. RESULTS: No significant differences were found between median test and retest scores on the JSQ. The median ratings for each sound-quality dimension were found to have moderate test-retest reliability, with test-retest correlations (r) ranging from 0.20 to 0.73 (median r value = 0.58) for the unaided listening condition, and from 0.23 to 0.85 (median r value = 0.51) for the aided listening condition. Test validity was established through significant differences in JSQ ratings for various stimulus pairs (e.g., speech in quiet versus speech in noise). In addition, significant differences were observed between unaided and aided ratings for the dimensions of clarity, nearness, loudness, and total impression with aided JSQ ratings approaching normative "ideal" values established previously. CONCLUSIONS: The JSQ appears to be a potentially useful measure of hearing aid outcome, especially when using group data to document the benefits of amplification. Additional efforts should be directed at improving the reliability of the JSQ, however, before application to hearing aid wearers on an individual basis.