A Scd1-mediated metabolic alteration participates in liver responses to low-dose bavachin.

Hepatotoxicity induced by bioactive constituents in traditional Chinese medicines or herbs, such as bavachin (BV) in Fructus Psoraleae, has a prolonged latency to overt drug-induced liver injury in the clinic. Several studies have described BV-induced liver damage and underlying toxicity mechanisms, but little attention has been paid to the deciphering of organisms or cellular responses to BV at no-observed-adverse-effect level, and the underlying molecular mechanisms and specific indicators are also lacking during the asymptomatic phase, making it much harder for early recognition of hepatotoxicity. Here, we treated mice with BV for 7 days and did not detect any abnormalities in biochemical tests, but found subtle steatosis in BV-treated hepatocytes. We then profiled the gene expression of hepatocytes and non-parenchymal cells at single-cell resolution and discovered three types of hepatocyte subsets in the BV-treated liver. Among these, the hepa3 subtype suffered from a vast alteration in lipid metabolism, which was characterized by enhanced expression of apolipoproteins, carboxylesterases, and stearoyl-CoA desaturase 1 (Scd1). In particular, increased Scd1 promoted monounsaturated fatty acids (MUFAs) synthesis and was considered to be related to BV-induced steatosis and polyunsaturated fatty acids (PUFAs) generation, which participates in the initiation of ferroptosis. Additionally, we demonstrated that multiple intrinsic transcription factors, including Srebf1 and Hnf4a, and extrinsic signals from niche cells may regulate the above-mentioned molecular events in BV-treated hepatocytes. Collectively, our study deciphered the features of hepatocytes in response to BV insult, decoded the underlying molecular mechanisms, and suggested that Scd1 could be a hub molecule for the prediction of hepatotoxicity at an early stage.

Excessive sulfur oxidation in endoplasmic reticulum drives an inflammatory reaction of chondrocytes in aging mice.

Osteoarthritis, as a common joint disease among middle-aged and elderly people, has many problems, such as diverse pathogenesis, poor prognosis and high recurrence rate, which seriously affects patients' physical and mental health and reduces their quality of life. At present, the pathogenesis of osteoarthritis is not completely clear, and the treatment plan is mainly to relieve symptoms and ensure basic quality of life. Therefore, it is particularly urgent to explore the pathogenesis of osteoarthritis. Protein, as organic macromolecule which plays a major role in life activities, plays an important role in the development of disease. Through protein omics, this study found that with the increase of age, excessive sulfur oxidation occurred in endoplasmic reticulum of chondrocytes, which then drove the occurrence of inflammatory reaction, and provided a direction for the follow-up molecular targeted.

Impact of solid digestate processing on carbon emission of an industrial-scale food waste co-digestion plant.

Anaerobic digestion (AD) has been widely applied for treating organic waste and is known as a carbon-offsetting process. However, most studies relied on laboratory-scale experiments or literature to calculate carbon emissions from AD process, and the impact of digestate processing was overlooked. This study assessed the carbon footprint for an industrial food waste co-digestion plant with operational data. The results indicated that carbon emission before digestate treatment is -88.5 ± 4.4 kg CO2-eq/t. The major source of carbon emission is electricity provision, followed by fuel combustion, unburned biogas, and fugitive gas emissions, while waste oil recovery and biogas utilization offset the carbon emissions. Considering digestate treatment and disposal options, the plant's net carbon emissions are as follows: -86.1 ± 6.2 kg CO2-eq/t (incineration) < -80.7 ± 6.5 kg CO2-eq/t (land application) < 6.7 ± 12.2 kg CO2-eq/t (landfilling). This work aims at providing a roadmap for making site-specific calculations of the carbon footprint for AD process.

Impact of Maternal Age on Singleton Birthweight in Frozen Embryo Transfer Cycles.

Background: Previous studies have investigated the effect of maternal age on assisted reproductive technology success rates. However, little is known about the relationship between maternal age and neonatal birthweight in frozen embryo transfer (FET) cycles. Whether maternal age influences singleton birthweight in FET cycles remains to be elucidated. Methods: This study was conducted at a tertiary care center, involving singleton live births born to women undergoing frozen-thawed embryo transfer during the period from January 2010 to December 2017. A total of 12,565 women who fulfilled the inclusion criteria were enrolled and grouped into four groups according to the maternal age: <30, 30-34, 35-39, and ≥40 years old. A multivariable linear regression analysis was conducted to reveal the relationship between maternal age and neonatal birthweight with controlling for a number of potential confounders. Results: The highest proportions of low birthweight (LBW, 4.1%), high birthweight (1.2%), preterm birth (PTB, 5.9%), and very PTB (0.9%) were found in the group over 40 years old, but no significant difference was observed among the four groups. Additionally, the 35-39-year-old group had the highest rate of very LBW (0.6%), whereas the 30-34-year-old group had the lowest rate of small for gestational age (SGA, 2.7%). However, multivariate analyses revealed that neonatal outcomes including PTB, LBW, and SGA were similar between the different maternal age groups. Conclusion: Grouping with different maternal age was not associated with mean birthweight and Z-scores of singletons resulting from FET.

Engineering Resistance to Bacterial Blight and Bacterial Leaf Streak in Rice.

BACKGROUND: Xanthomonas oryzae (Xo) is one of the important pathogenic bacterial groups affecting rice production. Its pathovars Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) cause bacterial blight and bacterial leaf streak in rice, respectively. Xo infects host plants by relying mainly on its transcription activator-like effectors (TALEs) that bind to host DNA targets, named effector binding elements (EBEs), and induce the expression of downstream major susceptibility genes. Blocking TALE binding to EBE could increase rice resistance to the corresponding Xo. FINDINGS: We used CRISPR/Cas9 to edit the EBEs of three major susceptibility genes (OsSWEET11, OsSWEET14 and OsSULTR3;6) in the rice varieties Guihong 1 and Zhonghua 11. Both varieties have a natural one-base mutation in the EBE of another major susceptibility gene (OsSWEET13) which is not induced by the corresponding TALE. Two rice lines GT0105 (from Guihong 1) and ZT0918 (from Zhonghua 11) with target mutations and transgene-free were obtained and showed significantly enhanced resistance to the tested strains of Xoo and Xoc. Furthermore, under simulated field conditions, the morphology and other agronomic traits of GT0105 and ZT0918 were basically the same as those of the wild types. CONCLUSIONS: In this study, we first reported that the engineering rice lines obtained by editing the promoters of susceptibility genes are resistant to Xoo and Xoc, and their original agronomic traits are not affected.

A novel redox-responsive ursolic acid polymeric prodrug delivery system for osteosarcoma therapy.

Ursolic acid (UA), found widely in nature, exerts effective anti-tumoral activity against various malignant tumors. However, the low water solubility and poor bioavailability of UA have greatly hindered its translation to the clinic. To overcome these drawbacks, a simple redox-sensitive UA polymeric prodrug was synthesized by conjugating UA to polyethylene glycol using a disulfide bond. This formulation can self-assemble into micelles (U-SS-M) in aqueous solutions to produce small size micelles (∼62.5 nm in diameter) with high drug loading efficiency (∼16.7%) that exhibit pH and reduction dual-sensitivity. The cell and animal studies performed using the osteosarcoma MG-63 cell line and MG-63 cancer xenograft mice as the model systems consistently confirmed that the U-SS-M formulation could significantly prolong the circulation in blood and favor accumulation in tumor tissue. Targeted accumulation allows the U-SS-M to be effectively internalized by cancer cells, where the rapid release of UA is favored by a glutathione-rich and acidic intracellular environment, and ultimately achieves potent antitumor efficacy.

Influence of temperature on enhancement of volatile fatty acids fermentation from organic fraction of municipal solid waste: Synergism between food and paper components.

This study explores individual contributions and synergistic effects of food and paper, main components of organic fraction of municipal solid waste (OFMSW) towards volatile fatty acids (VFA) fermentation under different temperatures (25, 37, 42 and 52 °C). Thanks to the synergism of food and paper component (FC & PC), the results revealed that OFMSW is suitable for VFA production. Maximum VFA production was noticed to be 21.5 mg/L at 42 °C, ~2.1, and 1.42 times higher than fermentation of PC and FC. Enhanced hydrolysis of PC occurred at >37 °C, increasing alkalinity in leachate to 6.7 g/L at 42 °C, thus maintaining a stable pH (5.4-5.6) during acidogenic fermentation. Additionally, 74% of COD is hydrolyzed, of which 79% is converted to VFA based on biodegradable carbon at 42 °C. It is suggested that co-existence of FC and PC can enhance VFA production of OFMSW, and targeted VFA production can be maximized through process optimization.

Type III effectors xopN and avrBS2 contribute to the virulence of Xanthomonas oryzae pv. oryzicola strain GX01.

Xanthomonas oryzae pv. oryzicola (Xoc) depends on its type III secretion system (T3SS) to translocate type III secreted effectors (T3SEs), including transcription activator-like effectors (TALEs) and non-transcription activator-like effectors (non-TALEs), into host cells. T3SEs can promote the colonization of Xoc and contribute to virulence by manipulating host cell physiology. We annotated 25 genes encoding non-TALEs in Xoc strain GX01, an isolate from Guangxi in the South China's rice growing region. Through systematic mutagenesis of non-TALEs, we found that xopN, the virulence contribution of which was previously unknown for Xoc, significantly contributes to the virulence of Xoc GX01, as does avrBs2.

Dual RNA-seq of Xanthomonas oryzae pv. oryzicola infecting rice reveals novel insights into bacterial-plant interaction.

The Gram-negative bacterium Xanthomonas oryzae pv. oryzicola (Xoc) is the causal agent of rice bacterial leaf streak (BLS), one of the most destructive diseases of rice (Oryza sativa L.) that is the important staple crop. Xoc can invade host leaves via stomata and wounds and its type three secretion system (T3SS) is pivotal to its pathogenic lifestyle. In this study, using a novel dual RNA-seq approach, we examined transcriptomes of rice and Xoc in samples inoculated with wild type Xoc GX01 and its T3SS defective strain (T3SD), to investigate the global transcriptional changes in both organisms. Compared with T3SD strain, rice inoculated with wild type Xoc GX01 resulted in significant expression changes of a series of plant defence related genes, including ones altered in plant signalling pathway, and downregulated in phenylalanine metabolism, flavonoid and momilactone biosynthesis, suggesting repression of plant defence response and reduction in both callose deposition and phytoalexin accumulation. Also, some known transcription activator-like effector (TALE) targets were induced by Xoc GX01, e.g. OsSultr3;6 which contributes to rice susceptibility. Some cell elongation related genes, including several expansin genes, were induced by GX01 too, suggesting that Xoc may exploit this pathway to weaken cell wall strength, beneficial for bacterial infection. On the other hand, compared with wild type, the T3SD strain transcriptome in planta was characterized by downregulation of ATP, protein and polysaccharide synthesis, and upregulation of antioxidation and detoxification related genes, revealing that T3SD strain faced serious starvation and oxidation stresses in planta without a functional T3SS. In addition, comparative global transcript profiles of Xoc in planta and in medium revealed an upregulation of virulence factor synthesis and secretion in planta in favour of bacterial infection. Collectively, this study provides a comprehensive representation of cross talk between the host and bacterial pathogen, revealing insights into the Xoc-rice pathogenic dynamic and reveals novel strategies exploited by this important pathogen to cause disease.

The effect of ISR on OFMSW during acidogenic fermentation for the production of AD precursor: kinetics and synergies.

Acidogenic fermentation of the organic fraction of municipal solid waste (OFMSW) and its components (food waste, paper waste) was studied in a batch percolator reactor without artificial pH adjustment. The effect of inoculum to substrate ratio on process performance, in terms of pH, hydrolysis and volatile fatty acid (VFA) production, has been investigated. The inoculum to substrate ratio (ISR) was varied from 0 to 0.36 VS/VS and at optimized conditions for fermentation of OFMSW, with ISR 0.23, pH, hydrolysis and acidification yield were 5.5, 625 mg sCOD per g BD VS and 408 mg g-1 BD VS respectively. Due to the uplift of pH from 4 to 5.5 because of addition of ISR, the VFA composition was dominated mostly by butyric, acetic acids and propionic acid. Kinetics regarding rate of hydrolysis and acidification were calculated and reported. A significant synergistic effect was noticed in the acidification and hydrolysis, which were 1.76 and 1.35 fold higher than individual components (paper waste and food waste) of OFMSW, respectively and approximately 70% of biodegradable solid carbon solubilized into the liquid carbon within a short retention time of 78 h.

Expression of miR-206 in Human Knee Articular Chondrocytes and Effects of miR-206 on Proliferation and Apoptosis of Articular Chondrocytes.

BACKGROUND: Increasing evidence has demonstrated that microRNAs regulate the development of cartilage and osteogenesis. Whether miR-206 participates in the development of human articular cartilage remains largely unknown. This study aimed to investigate the role of miR-206 in human chondrocytes. METHODS: Expression of miR-206 was initially assessed in human osteoarthritis (OA) tissues and articular chondrocytes through quantitative real-time polymerase chain reaction. The effects of miR-206 on proliferation and apoptosis of human chondrocytes were assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining assay. Then, the effects of miR-206 on type II collagen alpha 1 (Col2a1), aggrecan, runt-related transcription factor 2 (RUNX2) and matrix metalloproteinase13 (MMP13) were examined with quantitative real-time polymerase chain reaction and Western blot analysis. RESULTS: MiR-206 was significantly increased in human OA tissues and chondrocytes. MiR-206 significantly inhibited the proliferation of chondrocytes, but promoted apoptosis. Expression of Col2a1 and aggrecan were dramatically decreased, and the expression of RUNX2 and MMP13 were significantly increased when miR-206 was overexpressed. CONCLUSIONS: MiR-206 may participate in cartilage degradation in OA. Manipulation of the expression of miR-206 in human chondrocytes may be a novel therapeutic strategy for the treatment of OA.

Cementless total hip arthroplasty with extended sliding trochanteric osteotomy for high congenital hip dislocation: A retrospective study.

Total hip arthroplasty (THA) for high congenital hip dislocation (CHD) is technically demanding. The purpose of this retrospective study was to evaluate the results of cementless THA combined with extended sliding trochanteric osteotomy. We also assessed whether chronic low back pain was relieved after surgery.The study included 19 patients (23 hips) with high CHD treated with cementless THA using extended sliding trochanteric osteotomy technique. Clinical and radiographic outcomes were evaluated.Harris Hip Score, WOMAC score, visual analog scale for low back pain and Trendelenburg sign were significantly improved (P < 0.01) compared with the preoperative. Average limb-length discrepancy in the 15 unilateral hips was reduced from 38.2 ±â€Š7.9 mm to 6.7 ±â€Š4.1 mm (P < 0.01). No dislocation, deep vein thrombosis, or infection occurred. Two patients (8.7%) developed sciatic nerve palsy. One (4.3%) developed symptomatic greater trochanteric bursitis. Two (8.7%) sustained proximal femur shaft fracture during implantation of the femoral component. All femoral components showed successful bony ingrowth at the final follow-up. No stem subsidence was detected. There was no acetabular loosening. Bony union of the reattached greater trochanter was obtained in all hips. Wire breakage occurred in 3 hips (13%).Cementless THA with extended sliding trochanteric osteotomy may be appropriate options for patients with high CHD.

Effect of initial bulk density on high-solids anaerobic digestion of MSW: General mechanism.

Initial bulk density (IBD) is an important variable in anaerobic digestion since it defines and optimizes the treatment capacity of a system. This study reveals the mechanism on how IBD might affect anaerobic digestion of waste. Four different IBD values: D1 (500-700kgm-3), D2 (900-1000kgm-3), D3 (1100-1200kgm-3) and D4 (1200-1400kgm-3) were set and tested over a period of 90days in simulated landfill reactors. The main variables affected by the IBD are the methane generation, saturation degree, extraction of organic matter, and the total population of methanogens. The study identified that IBD >1000kgm-3 may have significant effect on methane generation, either prolonging the lag time or completely inhibiting the process. This study provides a new understanding of the anaerobic digestion process in saturated high-solids systems.

Shikonin inhibits inflammation and chondrocyte apoptosis by regulation of the PI3K/Akt signaling pathway in a rat model of osteoarthritis.

Shikonin has previously been shown to have antitumor, anti-inflammatory, antiviral and extensive pharmacological effects. The aim of the present study was to explore whether the protective effect of shikonin is mediated via the inhibition of inflammation and chondrocyte apoptosis, and to elucidate the potential molecular mechanisms in a rat model of osteoarthritis. A model of osteoarthritis was established in healthy male Sprague-Dawley rats and 10 mg/kg/day shikonin was administered intraperitoneally for 4 days. It was found that shikonin treatment significantly inhibited inflammatory reactions in the rats with osteoarthritis. Osteoarthritis was found to significantly increase interleukin (IL)-1ß, tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS) levels compared with those in the sham group. However, shikonin treatment significantly inhibited the increases in IL-1ß, TNF-α and iNOS levels in the rats with osteoarthritis. Furthermore, caspase-3 activity and cyclooxygenase (COX)-2 protein expression were significantly increased and phosphorylated Akt protein expression was greatly suppressed in rats with osteoarthritis when compared with the sham group. Shikonin administration attenuated the changes in caspase-3 activity and COX-2 expression and Akt phosphorylation in rats with osteoarthritis. These results indicate that shikonin inhibits inflammation and chondrocyte apoptosis by regulating the phosphoinositide 3-kinase/Akt signaling pathway in a rat model of osteoarthritis.

Targeted modification of organic components of municipal solid waste by short-term pre-aeration and its enhancement on anaerobic degradation in simulated landfill bioreactors.

Pre-aeration is effective on regulating subsequent anaerobic degradation of municipal solid waste (MSW) with high organic fractions during landfilling. The strength of pre-aeration should be optimized to intentionally remove some easily biodegradable fractions while conserve bio-methane potential as much as possible. This study investigates the evolution of organic components in MSW during 2-14days pre-aeration process and its impacts on subsequent anaerobic degradation in simulated landfill bioreactors. Results showed that a 6-day pre-aeration enabled to develop a thermophilic stage, which significantly accelerated biodegradation of organics except lignocelluloses, with removal rates of 42.8%, 76.7% and 25.1% for proteins, carbohydrates and lipids, respectively. Particularly, ammonia from accelerated ammonification in the thermophilic stage neutralized VFAs generated from anaerobic landfilling. As a result, the MSW with 6-day pre-aeration obtained the highest methane yield 123.4NL/kg dry matter. Therefore, it is recommended to interrupt pre-aeration before its cooling stage to switch to anaerobic landfilling.

Identification and characterization of odorous gas emission from a full-scale food waste anaerobic digestion plant in China.

Odorous gas emission characteristic along with the successive processes of a typical full-scale food waste (FW) anaerobic digestion plant in China was investigated in September and January. Seasonal variations in pollutant concentration and principal component analysis (PCA) showed markedly different characteristics between the two months. However, the main reason for the seasonal difference at the sorting process differed from the reason for the seasonal difference at other treatment units. Most odorous volatile organic compound (VOC) concentrations tested near an anaerobic digestion tank were similar and low in both months. Odor indices, including odor contribution (OC) and odor activity value (OAV) of various odorants, were further calculated to evaluate the malodor degree and contribution to the nuisance smell of any odorant. Brought about by people's different dietary habits, H2S and sulfocompounds were found to be dominant contributors to the large total OVA in the January test. By contrast, oxygenated organic compounds played an important role on the sum of OVA in September.

Evaluating the impact of odors from the 1955 landfills in China using a bottom-up approach.

Landfill odors have created a major concern for the Chinese public. Based on the combination of a first order decay (FOD) model and a ground-level point source Gaussian dispersion model, the impacts from odors emitted from the 1955 landfills in China are evaluated in this paper. Our bottom-up approach uses basic data related to each landfill to achieve a more accurate and comprehensive understanding of impact of landfill odors. Results reveal that the average radius of impact of landfill odors in China is 796 m, while most landfills (46.85%) are within the range of 400-1000 m, in line with the results from previous studies. The total land area impacted by odors has reached 837,476 ha, accounting for 0.09% of China's land territory. Guangdong and Sichuan provinces have the largest land areas impacted by odors, while Tibet Autonomous Region and Tianjin Municipality have the smallest. According to the CALPUFF (California Puff) model and an analysis of social big data, the overall uncertainty of our calculation of the range of odor impacts is roughly -32.88% to 32.67%. This type of study is essential for gaining an accurate and detailed estimation of the affected human population and will prove valuable for addressing the current Not In My Back Yard (NIMBY) challenge in China.

Characterization of odorous charge and photochemical reactivity of VOC emissions from a full-scale food waste treatment plant in China.

Food waste treatment plants (FWTPs) are usually associated with odorous nuisance and health risks, which are partially caused by volatile organic compound (VOC) emissions. This study investigated the VOC emissions from a selected full-scale FWTP in China. The feedstock used in this plant was mainly collected from local restaurants. For a year, the FWTP was closely monitored on specific days in each season. Four major indoor treatment units of the plant, including the storage room, sorting/crushing room, hydrothermal hydrolysis unit, and aerobic fermentation unit, were chosen as the monitoring locations. The highest mean concentration of total VOC emissions was observed in the aerobic fermentation unit at 21,748.2-31,283.3 µg/m3, followed by the hydrothermal hydrolysis unit at 10,798.1-23,144.4 µg/m3. The detected VOC families included biogenic compounds (oxygenated compounds, hydrocarbons, terpenes, and organosulfur compounds) and abiogenic compounds (aromatic hydrocarbons and halocarbons). Oxygenated compounds, particularly alcohols, were the most abundant compounds in all samples. With the use of odor index analysis and principal components analysis, the hydrothermal hydrolysis and aerobic fermentation units were clearly distinguished from the pre-treatment units, as characterized by their higher contributions to odorous nuisance. Methanthiol was the dominant odorant in the hydrothermal hydrolysis unit, whereas aldehyde was the dominant odorant in the aerobic fermentation unit. Terpenes, specifically limonene, had the highest level of propylene equivalent concentration during the monitoring periods. This concentration can contribute to the increase in the atmospheric reactivity and ozone formation potential in the surrounding air.

[Short-term curative effects of Tantalum rod treatment in early avascular necrosis].

OBJECTIVE: To explore the recent clinical curative effect of Tantalum rod in treating the early avascular necrosis. METHODS: From January 2008 to November 2008, the 25 patients (39 hips) with early avascular necrosis accepted tantalum rod placement and included 9 males (11 hips) and 16 females (28 hips) with an average age of 37 years old ranging from 18 to 74 years old. Four patients (6 hips) caused by Alcoholic, 6 patients (8 hips) by hormone, 2 cases (2 hips) by traumatic, 13 cases (23 hips) by idiopathic. Steinberg preoperative stage involved 7 hips in period I, 24 hips in period II, 8 hips in period III. Curative effect analysis included preoperative and postoperative Harris score, radiographic changes and hip replacement for follow-up to accept the end of the femoral head survival rate. RESULTS: All patients were followed up for 6 to 47 months (averaged 37.4 months). All 12 hips imaging appeard progress,including tantalum rod exit in 1 hip, hip hemiarthroplasty collapse in 3 hips, the area increased to avascular necrosis in 8 hips. Six hips accepted total hip replacement, including imaging progress in 5 hips (41.7%, 5/12), no imaging progress in 1 hip (3.7%,1/27). All hips' Kaplan-Meier survival curves showed 6-month survival rate was (97.4 +/- 2.5)% after tantalum stick insertion, 1-year survival rate was (94.7 +/- 3.6), and 2-year survival rate was (88.6 +/- 5.4)%, 3-year survival rate was (72.5 +/- 11.2). CONCLUSION: It is effective for treatment of avascular necrosis of femoral head in Steinberg I and II by Tantalum rod, and it can effectively relieve femoral head replacement time.

Layered double hydroxides as efficient photocatalysts for visible-light degradation of Rhodamine B.

A series of Zn/M-NO3-LDHs (M=Al, Fe, Ti, and Fe/Ti) have been synthesized by two different methods, and their activities for visible-light photocatalytic degradation on Rhodamine B (RB) were tested. Solids were analyzed by XRD, FT-IR, and ICP characterization, confirming the formation of pure LDH phase with good crystal structure. It was observed that the band gap of these nitrate LDH materials was following this order: Zn/Fe-NO3-LDHs (2.55 eV)>Zn/Fe/Ti-NO3-LDHs (2.88 eV)>Zn/Ti-NO3-LDHs (3.0 3eV)>Zn/Al-NO3-LDHs (3.23 eV); however, the degradation performance of RB by four materials followed the order: Zn/Ti-NO3-LDHs (98%)>Zn/Al-NO3-LDHs (96%)>Zn/Fe/Ti-NO3-LDHs (88%)>Zn/Fe-NO3-LDHs (72%). In addition, a possible mechanism for photocatalytic degradation on RB has also been presumed. Moreover, after three regeneration cycles, the percentage of RB degradation rate was still close to 90%.