Cyclophosphamide induces ovarian granulosa cell ferroptosis via a mechanism associated with HO-1 and ROS-mediated mitochondrial dysfunction.

Abnormal granulosa cell (GC) death contributes to cyclophosphamide (CTX) induced primary ovarian insufficiency (POI). To investigate the contribution of GCs to POI, gene profiles of GCs exposed to CTX were assessed using RNA-Seq and bioinformatics analysis. The results showed the differentially expressed genes (DEGs) were enriched in the ferroptosis-related pathway, which is correlated with upregulated heme oxygenase 1 (HO-1) and downregulated glutathione peroxidase-4 (GPX4). Using CTX-induced cell culture (COV434 and KGN cells), the levels of iron, reactive oxygen species (ROS), lipid peroxide, mitochondrial superoxide, mitochondrial morphology and mitochondrial membrane potential (MMP) were detected by DCFDA, MitoSOX, C11-BODIPY, MitoTracker, Nonylacridine Orange (NAO), JC-1 and transmission electron microscopy respectively. The results showed iron overload and disrupted ROS, including cytoROS, mtROS and lipROS homeostasis, were associated with upregulation of HO-1 and could induce ferroptosis via mitochondrial dysfunction in CTX-induced GCs. Moreover, HO-1 inhibition could suppress ferroptosis induced GPX4 depletion. This implies a role for ROS in CTX-induced ferroptosis and highlights the effect of HO-1 modulators in improving CTX-induced ovarian damage, which may provide a theoretical basis for preventing or restoring GC and ovarian function in patients with POI.

KAT8/SIRT7-mediated Fascin-K41 acetylation/deacetylation regulates tumor metastasis in esophageal squamous cell carcinoma.

Fascin actin-bundling protein 1 (Fascin) is highly expressed in a variety of cancers, including esophageal squamous cell carcinoma (ESCC), working as an important oncogenic protein and promoting the migration and invasion of cancer cells by bundling F-actin to facilitate the formation of filopodia and invadopodia. However, it is not clear how exactly the function of Fascin is regulated by acetylation in cancer cells. Here, in ESCC cells, the histone acetyltransferase KAT8 catalyzed Fascin lysine 41 (K41) acetylation, to inhibit Fascin-mediated F-actin bundling and the formation of filopodia and invadopodia. Furthermore, NAD-dependent protein deacetylase sirtuin (SIRT) 7-mediated deacetylation of Fascin-K41 enhances the formation of filopodia and invadopodia, which promotes the migration and invasion of ESCC cells. Clinically, the analysis of cancer and adjacent tissue samples from patients with ESCC showed that Fascin-K41 acetylation was lower in the cancer tissue of patients with lymph node metastasis than in that of patients without lymph node metastasis, and low levels of Fascin-K41 acetylation were associated with a poorer prognosis in patients with ESCC. Importantly, K41 acetylation significantly blocked NP-G2-044, one of the Fascin inhibitors currently being clinically evaluated, suggesting that NP-G2-044 may be more suitable for patients with low levels of Fascin-K41 acetylation, but not suitable for patients with high levels of Fascin-K41 acetylation. © 2024 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

A study on the role of serum uric acid in differentiating acute inflammatory demyelinating polyneuropathy from acute-onset chronic inflammatory demyelinating polyneuropathy.

BACKGROUND AND PURPOSE: Clinical symptoms and laboratory indices for acute inflammatory demyelinating polyneuropathy (AIDP), a variant of Guillain-Barré syndrome, and acute-onset chronic inflammatory demyelinating polyneuropathy (A-CIDP) were analyzed to identify factors that could contribute to early differential diagnosis. METHODS: A retrospective chart review was performed on 44 AIDP and 44 A-CIDP patients looking for any demographic characteristics, clinical manifestations or laboratory parameters that might differentiate AIDP from acutely presenting CIDP. RESULTS: In Guillain-Barré syndrome patients (N = 63), 69.84% (N = 44) were classified as having AIDP, 19.05% (N = 12) were found to have acute motor axonal neuropathy, 6.35% (N = 4) were found to have acute motor and sensory axonal neuropathy, and 4.76% (N = 3) were found to have Miller Fisher syndrome. Serum uric acid (UA) was higher in A-CIDP patients (329.55 ± 72.23 µmol/L) than in AIDP patients (221.08 ± 71.32 µmol/L) (p = 0.000). Receiver operating characteristic analyses indicated that the optimal UA cutoff was 283.50 µmol/L. Above this level, patients were more likely to present A-CIDP than AIDP (specificity 81.80%, sensitivity 81.80%). During the follow-up process, serum samples were effectively collected from 19 AIDP patients during the rehabilitation phase and 28 A-CIDP patients during the remission stage, and it was found that UA levels were significantly increased in A-CIDP (remission) (298.9 ± 90.39 µmol/L) compared with AIDP (rehabilitation) (220.1 ± 108.2 µmol/L, p = 0.009). CONCLUSION: These results suggest that serum UA level can help to differentiate AIDP from A-CIDP with high specificity and sensitivity, which is helpful for early diagnosis and guidance of treatment.

Design and preparation of an artificial vascular scaffold with internal surface modification.

BACKGROUND: Advances in regeneration methods have brought us improved vascular scaffolds with small diameters (φ < 6 mm) for enhancing biological suitability that solve their propensity for causing intimal hyperplasia post-transplantation. METHODS: The correlation between the rehydration ratio of the hydrogel and its material concentration is obtained by adjusting the material ratio of the hydrogel solution. The vascular model with helical structure has been established and analyzed to verify the effect of helical microvascular structure on thrombosis formation by the fluid simulation methods. Then, the helical structure vascular has been fabricated by self-developed 3D bioprinter, the vascular scaffolds are freeze-dried and rehydrated in polyethylene glycol (PEG) solution. RESULTS: The experimental results showed that the hybrid hydrogel had a qualified rehydration ratio when the content of gelatin, sodium alginate, and glycerol was 5, 6, and 3 wt%. The established flow channel model can effectively reduce thrombus deposition and improve long-term patency ratio. After PEG solution modification, the contact angle of the inner wall of the vascular scaffold was less than 30°, showing better hydrophilic characteristics. CONCLUSION: In study, a small-diameter inner wall vascular scaffold with better long-term patency was successfully designed and prepared by wrinkling and PEG modification of the inner wall of the vascular scaffold. This study not only creates small-diameter vascular scaffolds with helical structure that improves the surface hydrophilicity to reduce the risk of thrombosis but also rekindles confidence in the regeneration of small caliber vascular structures.

High-Performance Layered CaV4O9-MXene Composite Cathodes for Aqueous Zinc Ion Batteries.

Due to their reliability, affordability and high safety, rechargeable aqueous zinc ion batteries (ZIBs) have garnered a lot of attention. Nevertheless, undesirable long-term cycle performance and the inadequate energy density of cathode materials impede the development of ZIBs. Herein, we report a layered CaV4O9-MXene (Ti3C2Tx) composite assembled using CaV4O9 nanosheets on Ti3C2Tx and investigate its electrochemical performance as a new cathode for ZIBs, where CaV4O9 nanosheets attached on the surface of MXene and interlamination create a layered 2D structure, efficiently improving the electrical conductivity of CaV4O9 and avoiding the stacking of MXene nanosheets. The structure also enables fast ion and electron transport. Further discussion is conducted on the effects of adding MXene in various amounts on the morphology and electrochemical properties. The composite shows an improved reversible capacity of 274.3 mA h g-1 at 0.1 A g-1, superior rate capabilities at 7 A g-1, and a high specific capacity of 107.6 mA h g-1 can be delivered after 2000 cycles at a current density of 1 A g-1. The improvement of the electrochemical performance is due to its unique layered structure, high electrical conductivity, and pseudo capacitance behavior.

Graphene electrochemical transistors decorated by Ag nanoparticles exhibiting high sensitivity for the detection of paraquat over a wide concentration range.

Paraquat (PQ) is a nonselective contact herbicide used in agriculture for the control of broad leaf weeds, which would cause irreversible damage to human organs even at very low concentrations. Therefore, the trace residue detection of PQ in the environment is of vital importance. Here, a novel graphene electrochemical transistor (GECT) for PQ detection is reported for the first time. The key to the device design is the application of a layer of Ag nanoparticle (Ag NP) modified monolayer graphene as the channel layer. Due to the good electrochemical activity of Ag NPs for PQ detection, the device exhibits excellent sensitivity for PQ with the detection limit of 0.068 nM and a wide linear range from 0.1 nM to 5 mM. The GECT sensor also reveals good selectivity toward several common interferents and exhibits satisfactory recoveries for PQ detection when using Chinese cabbage as a simulant to deduce the real detection situation. The GECT sensor not only provides an efficient method for the detection of PQ residues, but also provides an effective grafting platform for the construction of novel high-sensitivity electrochemical sensors.

Effect of Hydrogel Contact Angle on Wall Thickness of Artificial Blood Vessel.

Vascular replacement is one of the most effective tools to solve cardiovascular diseases, but due to the limitations of autologous transplantation, size mismatch, etc., the blood vessels for replacement are often in short supply. The emergence of artificial blood vessels with 3D bioprinting has been expected to solve this problem. Blood vessel prosthesis plays an important role in the field of cardiovascular medical materials. However, a small-diameter blood vessel prosthesis (diameter < 6 mm) is still unable to achieve wide clinical application. In this paper, a response surface analysis was firstly utilized to obtain the relationship between the contact angle and the gelatin/sodium alginate mixed hydrogel solution at different temperatures and mass percentages. Then, the self-developed 3D bioprinter was used to obtain the optimal printing spacing under different conditions through row spacing, printing, and verifying the relationship between the contact angle and the printing thickness. Finally, the relationship between the blood vessel wall thickness and the contact angle was obtained by biofabrication with 3D bioprinting, which can also confirm the controllability of the vascular membrane thickness molding. It lays a foundation for the following study of the small caliber blood vessel printing molding experiment.

V5+-Doped Potassium Ferrite as an Efficient Trifunctional Catalyst for Large-Current-Density Water Splitting and Long-Life Rechargeable Zn-Air Battery.

Developing non-noble metal catalyst with super trifunctional activities for efficient overall water splitting (OWS) and rechargeable Zn-air battery (ZAB) is urgently needed. However, catalysts with excellent oxygen evolution reaction (OER), oxygen reduction reaction (ORR), and hydrogen evolution reaction (HER) performances are relatively few. Although metal-ionic-conductor K2Fe4O7 (KFO) can output large current densities for OER/HER even in 10.0 M KOH electrolyte, its water-splitting property still needs to be further improved. Herein, we introduced V5+ directly into KFO and synthesized the binder-free nickel foam (NF) basal V-KFO nanoparticles (labeled as V-KFO/NF). Both the theoretical analysis and actual experimental data certify that V5+ doping enhances the instinct water-splitting property of V-KFO/NF. Additionally, V-KFO/NF can directly serve as the air cathode of liquid/flexible ZABs. The assembled liquid ZAB can continue the charge-discharge cycling testing with a lower voltage gap (0.834 V) and a longer operation life (>550 h) at 10 mA cm-2. Meanwhile, the assembled flexible ZAB can drive the two-electrode water-splitting unit of V-KFO/NF and needs only 1.54 V to achieve the current density of 10 mA cm-2, which is much lower than that of KFO/NF (1.59 V). This work not only provides a novel and efficient trifunctional catalyst for a self-powered water-splitting device but also is the foundation support for other heteroatom-doped low-cost materials.

Insights into the Underlying Mechanism of Ochratoxin A Production in Aspergillus niger CBS 513.88 Using Different Carbon Sources.

Aspergillus niger produces carcinogenic ochratoxin A (OTA), a serious food safety and human health concern. Here, the ability of A. niger CBS 513.88 to produce OTA using different carbon sources was investigated and the underlying regulatory mechanism was elucidated. The results indicated that 6% sucrose, glucose, and arabinose could trigger OTA biosynthesis and that 1586 differentially expressed genes (DEGs) overlapped compared to a non-inducing nutritional source, peptone. The genes that participated in OTA and its precursor phenylalanine biosynthesis, including pks, p450, nrps, hal, and bzip, were up-regulated, while the genes involved in oxidant detoxification, such as cat and pod, were down-regulated. Correspondingly, the activities of catalase and peroxidase were also decreased. Notably, the novel Gal4-like transcription factor An12g00840 (AnGal4), which is vital in regulating OTA biosynthesis, was identified. Deletion of AnGal4 elevated the OTA yields by 47.65%, 54.60%, and 309.23% using sucrose, glucose, and arabinose as carbon sources, respectively. Additionally, deletion of AnGal4 increased the superoxide anion and H2O2 contents, as well as the sensitivity to H2O2, using the three carbon sources. These results suggest that these three carbon sources repressed AnGal4, leading to the up-regulation of the OTA biosynthetic genes and alteration of cellular redox homeostasis, ultimately triggering OTA biosynthesis in A. niger.

Influence of zinc levels and Nrf2 expression in the clinical and pathological changes in patients with diabetic nephropathy.

OBJECTIVE: We investigated the correlation between zinc levels and Nrf2 expression and potential effects on the clinicopathology of patients with diabetic nephropathy (DN). METHODS: We selected 30 patients with DN, diagnosed via renal biopsy at our hospital from March 2018 to November 2019, and enrolled 30 healthy individuals from a medical examination center as the control group. Patients with DN were divided into normal-zinc and low-zinc groups. We detected the levels of zinc, copper, and Nrf2 mRNA in their serum, and collected the clinical and pathological data of DN patients. RESULTS: Serum zinc level and Nrf2 mRNA expression were significantly decreased in patients with DN compared to those of healthy people (P < 0.05). Of the 30 patients, 16 had low zinc (53.3%) and 14 had normal zinc levels (46.7%). There was no significant difference in the blood Nrf2 mRNA expression between the two groups (P > 0.05). However, the expression of Nrf2 in the kidney tissue of the low-zinc group was significantly lower compared to the normal-zinc group (P < 0.05). Diastolic blood pressure and copper levels were significantly higher in the low-zinc group (P < 0.05). In contrast, body mass index, red blood cell count, Hb level, and the ratio of zinc to copper were significantly lower in the low-zinc group (P < 0.05). The pathological classifications of the low-zinc group were more severe (P < 0.05). CONCLUSION: Patients with DN were more likely to have zinc deficiency and lower expression of Nrf2. Additionally, DN patients with zinc deficiency were prone to have more severe clinical and pathological manifestations.

Highly Dispersed Antimony-Bismuth Alloy Encapsulated in Carbon Nanofibers for Ultrastable K-Ion Batteries.

Antimony-based alloys have appealed to an ever-increasing interest for potassium ion storage due to their high theoretical capacity and safe voltage. However, sluggish kinetics and the large radius of K+ lead to limited rate performance and severe capacity fading. In this Letter, highly dispersed antimony-bismuth alloy nanoparticles confined in carbon fibers are fabricated through an electrospinning technology followed by heat treatment. The BiSb nanoparticles are uniformly confined into the carbon fibers, which facilitate rapid electron transport and inhibit the volume change during cycling owing to the synergistic effect of the BiSb alloy and carbon confinement engineering. Furthermore, the effect of a potassium bis(fluorosulfonyl)imide (KFSI) electrolyte with different concentrations has been investigated. Theoretical calculation demonstrates that the incorporation of Bi metal is favorable for potassium adsorption. The combination of delicate nanofiber morphology and electrolyte chemistry endows the fiber composite with an improved reversible capacity of 274.4 mAh g-1, promising rate capability, and cycling stability upon 500 cycles.

Antidiarrheal effect of the ethanol extract from Scutellaria barbata and its effect on the contraction of jejunum smooth muscles.

Scutellaria barbata (S. barbata), a traditional herbal medicine used in southern China, possesses anti-inflammatory, antitumor, spasmolytic and expectorant effects. However, there are not many recent studies on its gastrointestinal effects. This study aimed to evaluate the antidiarrheal effect of the ethanol extract of S. barbata (SBE) and its effect on the isolated jejunum smooth muscle. METHODS: The antidiarrheal effect of SBE (doses: 125, 250 and 500 mg/kg) on castor oil-induced diarrhea was investigated in vivo. The effect of SBE (0.01-10 mg/mL) on spontaneous or acetylcholine chloride (ACh, 10µM)/KCl (60mM)-induced contraction of isolated rabbit jejunum smooth muscle was examined in vitro. The possible spasmolytic mechanism of SBE (1 and 3mg/mL) was analyzed by accumulating CaCl2 in a Ca2+-free high-K+ (60mM) solution. RESULTS: SBE (125, 250 and 500mg/kg) could delay the initial semi-solid onset time of mice and also reduce the diarrhea index in vivo. Furthermore, SBE (0.01-10mg/mL) could alleviate the spontaneous or ACh/KCl-induced contraction in vitro. SBE (1 and 3mg/mL) also inhibited the contraction induced by CaCl2, and the concentration-response curves of CaCl2 moved downward and to the right, similar to those of verapamil (0.01 and 0.1µM). CONCLUSIONS: SBE exerts antidiarrheal and spasmolytic effects, which provides a pharmacological basis for its use in functional gastrointestinal disorders.

SS-31, a Mitochondria-Targeting Peptide, Ameliorates Kidney Disease.

Mitochondria are essential for eukaryotic cell activity and function, and their dysfunction is associated with the development and progression of renal diseases. In recent years, there has been a rapid development in mitochondria-targeting pharmacological strategies as mitochondrial biogenesis, morphology, and function, as well as dynamic changes in mitochondria, have been studied in disease states. Mitochondria-targeting drugs include nicotinamide mononucleotide, which supplements the NAD+ pool; mitochondria-targeted protective compounds, such as MitoQ; the antioxidant coenzyme, Q10; and cyclosporin A, an inhibitor of the mitochondrial permeability transition pore. However, traditional drugs targeting mitochondria have limited clinical applications due to their inability to be effectively absorbed by mitochondria in vivo and their high toxicity. Recently, SS-31, a mitochondria-targeting antioxidant, has received significant research attention as it decreases mitochondrial reactive oxygen species production and prevents mitochondrial depolarization, mitochondrial permeability transition pore formation, and Ca2+-induced mitochondrial swelling, and has no effects on normal mitochondria. At present, few studies have evaluated the effects of SS-31 against renal diseases, and the mechanism underlying its action is unclear. In this review, we first discuss the pharmacokinetics of SS-31 and the possible mechanisms underlying its protective effects against renal diseases. Then, we analyze its renal disease-improving effects in various experimental models, including animal and cell models, and summarize the clinical evidence of its benefits in renal disease treatment. Finally, the potential mechanism underlying the action of SS-31 against renal diseases is explored to lay a foundation for future preclinical studies and for the evaluation of its clinical applications.

Dealloying Synthesis of Silicon Nanotubes for High-Performance Lithium Ion Batteries.

The front cover artwork is provided by Dr. Ping Nie and Prof. Limin Chang at Jilin Normal University. The image shows one-dimensional silicon-nitrogen-doped carbon nanotube composite fabricated through a dealloying process. The nanotube engineered silicon coupled with conductive carbon coating synergistically boosts the electrochemical performance. Read the full text of the Research Article at 10.1002/cphc.202100832.

[Preliminary study on the effect of combined invisible orthodontic and orthognathic treatment in 24 skeletal Class â ¢ patients with facial asymmetry].

PURPOSE: To evaluate the clinical effect of combined orthodontic and orthognathic treatment with invisible aligner technique without brackets in skeletal Class Ⅲ patients with facial asymmetry. METHODS: A total of 24 skeletal Class Ⅲ patients with facial asymmetry treated with combined orthodontic and orthognathic treatment during the past 4 years were reviewed. Patients in the experimental group(n=12) were treated with invisible aligner technique without brackets, while patients in the control group(n=12) were treated with traditional fixed orthodontic technique for pre- and post-operative orthodontic treatment respectively. The cephalometric parameters and satisfaction questionnaire scores of the two groups before and after treatment were compared and analyzed with SPSS 20.0 software package for t test and Wilcoxon rank sum test, respectively. RESULTS: After treatment, the cephalometric parameters of SNA, SNB, ANB, U1-SN and L1-MP values were changed significantly(P＜0.05), but there was no significant difference between the values of experimental group and the control group(P＞0.05). The scores of aesthetics, comfort, portability, masticatory and speech function in the experimental group were significantly higher than those in the control group(P＜0.05). The satisfaction scores of the two groups were both 8.8±0.5(P＞0.05). CONCLUSIONS: Skeletal Class Ⅲ patients with facial asymmetry could obtain good clinical effect by using invisible aligner technique. The patients were satisfied with the aesthetics, comfort and the effect of combined invisible orthodontic and orthognathic treatment.

Administration of mesenchymal stem cells in diabetic kidney disease: mechanisms, signaling pathways, and preclinical evidence.

Diabetic kidney disease (DKD) is a serious microvascular complication of diabetes. Currently, the prevalence and mortality of DKD are increasing annually. However, with no effective drugs to prevent its occurrence and development, the primary therapeutic option is to control blood sugar and blood pressure. Therefore, new and effective drugs/methods are imperative to prevent the development of DKD in patients with diabetes. Mesenchymal stem cells (MSCs) with multi-differentiation potential and paracrine function have received extensive attention as a new treatment option for DKD. However, their role and mechanism in the treatment of DKD remain unclear, and clinical applications are still being explored. Given this, we here provide an unbiased review of recent advances in MSCs for the treatment of DKD in the last decade from the perspectives of the pathogenesis of DKD, biological characteristics of MSCs, and different molecular and signaling pathways. Furthermore, we summarize information on combination therapy strategies using MSCs. Finally, we discuss the challenges and prospects for clinical application.

Dealloying Synthesis of Silicon Nanotubes for High-Performance Lithium Ion Batteries.

Practical applications of silicon-based anodes in lithium ion batteries have attracted unprecedented attentions due to the merits of extraordinary energy density, high safety and low cost. Nevertheless, the inevitable huge volume change upon lithiation and delithiation brings about silicon electrode integrity damage and fast capacity fading, hampering the large-scale application. Herein, a novel one-dimensional tubular silicon-nitrogen doped carbon composite (Si@NC) with a core-shell structure has been fabricated using silicon magnesium alloy and polydopamine as a template and precursor. The as-obtained composite exhibits remarkable specific capacity and ultrafast redox kinetics, an outstanding cycling stability with fine capacity of 583.6 mAh g-1 at 0.5 A g-1 over 200 cycles is delivered. Moreover, a full cell matched with LiFePO4 cathode has demonstrated a reversible capacity of 148.8 mAh g-1 with high Coulombic efficiency as well as an excellent energy density of 396 Wh kg-1 . The nanotube structure engineering and silicon confined in nitrogen doped carbon effectively alleviate the volume expansion and endow the composite with superior stability. The robust strategy developed here gives a new insight into designing silicon anodes for enhanced lithium storage properties.

Cellular crosstalk of glomerular endothelial cells and podocytes in diabetic kidney disease.

Diabetic kidney disease (DKD) is a serious microvascular complication of diabetes and is the leading cause of end-stage renal disease (ESRD). Persistent proteinuria is an important feature of DKD, which is caused by the destruction of the glomerular filtration barrier (GFB). Glomerular endothelial cells (GECs) and podocytes are important components of the GFB, and their damage can be observed in the early stages of DKD. Recently, studies have found that crosstalk between cells directly affects DKD progression, which has prospective research significance. However, the pathways involved are complex and largely unexplored. Here, we review the literature on cellular crosstalk of GECs and podocytes in the context of DKD, and highlight specific gaps in the field to propose future research directions. Elucidating the intricates of such complex processes will help to further understand the pathogenesis of DKD and develop better prevention and treatment options.

Encapsulating silicon particles by graphitic carbon enables High-performance Lithium-ion batteries.

Silicon combines the advantages of high theoretical specific capacity, low potential and natural abundance, which exhibits great promise as an anode for lithium-ion batteries. However, the main challenges associated with Si anode are continuous volume expansion upon cycling and intrinsic low electronic conductivity, leading to sluggish reaction kinetics and rapid capacity fading. Herein we propose a novel in-situ self-catalytic strategy for the growth of highly graphitic carbon to encapsulate Si nanoparticles by chemical vapor deposition, where the magnesiothermic reduction byproducts are used as templates and catalysts for the formation of three-dimensional (3D) conductive network architecture. Benefiting from the improved electronic conductivity and significant suppression of volume expansion, the as-synthesized Si carbon composites exhibit excellent lithium storage capabilities in terms of high specific capacity (2126 mAh g-1 at 0.1 A g-1), remarkable rate capability (750 mAh g-1 at 5 A g-1), and good cycling stability over 450 cycles. Furthermore, the as-fabricated full cell (Si//Ni-rich LiNi0.815Co0.185-xAlxO2) shows high energy density of 395.1 Wh kg-1 and long-term stable cyclability. Significantly, this work demonstrates the effectiveness of in-situ self-catalysis reaction by using magnesiothermic reduction byproducts catalytically derived carbon matrix to encapsulate alloy-type anode material in giving rise to the overall energy storage performance.