[Biological Properties of Nocardia vaccinii IMV B-7405 Surfactants Synthesized on Fried Sunflower Oil].

Aim: To study of antimicrobial and antiadhesive properties of the surface-active substances (surfactants) Nocardia vaccinii IMV B-7405 synthesized on different oil-containing substrates (refined and waste after frying meat and potatoes sunflower oil). Methods: Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2 : 1). Antimicrobial against bacteria and yeast properties of the surfactant was determined by index of the minimum inhibitory concentration (MIC). The number (%) of attached cells (adhesion) was determined as a ratio of the optical density of the suspension obtained from the materials treated with surfactants to the optical density of the control samples (100 %). Results: It was established that surfactants synthesized N. vaccinii IMV B-7405 on fried oil, at a concentration of 0.02 - 0.04 mg/ml decreased adhesion of bacteria (Escherichia coli IEM-1, Bacillus subtilis BT-2) on plastic, dutch tile, glass and linoleum by 25-90 %, and yeast Candida albicans Д-6 - by 15 - 65 %. The minimum inhibitory concentration with respect to studied test cultures surfactants synthesized on waste after frying potato oil was 8 - 67 mg/ml, and against phytopathogenic bacteria Pectobacterium carotovorum UCM B-1095, Pseudomonas syringae pv. atrofaciens UCM B-1015 and Xanthomonas campestris pv. campestris UCM B-1049 - 14 - 52 mg/ml. Conclusions: Replacing traditional substrates on fried oil can not only reduce cost of N. vaccinii IMV B-7405 surfactant biosynthesis, but also to obtain the final product with high antiadhesive and antimicrobial properties. The dependence of antiadhesive and antimicrobial potential of surfactants on the nature of the carbon source indicate the need for studies effect of cultivation conditions of producer on biological properties of synthesized surfactants.

[Biological Properties of Nocardia vaccinii IMV B-7405 Surfactants Synthesized on Byproduct of Biodiesel Production].

Aim: Comparison of antimicrobial and anti-adhesive activity of Nocardia vaccinii ІMV B-7405 surfactants synthesized on purified and technical glycerol (byproduct of biodiesel production). Methods: Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2:1). Antimicrobial against bacteria and yeast properties of the surfactant was determined by index of the minimum inhibitory concentration (MIC). The number of attached cells was determined spectrophotometrically. Results: The dependence of surfactant antimicrobial and anti-aghesive activity on the degree of glycerol purification (purified, technical), as well as the duration of N. vaccinii IMV B-7405 cultivation on these substrates was established. MIC against studied test cultures surfactants, synthesized on technical glycerol during 5 days, was 15­121 µg/ml, that lower than MIC of surfactant obtained on purified substrate (22.5−180 µg/ml). Increasing duration of N. vaccinii ІMV B-7405 cultivation accompanied by rise the MIC against some test cultures surfactants synthesized on both technical and purified glycerol. Adhesion of the bacteria Escherichia coli ІEM-1, Bacillus subtilis BT-2 (vegetative cells and spores) and the yeast Candida albicans Д-6 on abiotic surfaces treated with surfactants synthesized on technical glycerol for both 5 and 7 days, was an average of 11−12 % less than after materials treatment with surfactants obtained on the purified substrate. Conclusions: Increasing activity of NADP+-dependent glutamate dehydrogenase − key enzyme of aminolipids biosynthesis (effective antimicrobial and anti-adhesion agents) in the presence of K+ and Na+ may indicate the possibility of biosynthesis intensification of these components of surfacrants complex under N. vaccinii ІMV B-7405 cultivation on technical glycerol, which is characterized by a high content of sodium and potassium cations. Replacing refined glycerol on byproduct of biodiesel production will not only reduce the cost of N. vaccinii ІMV B-7405 surfactant biosynthesis, but also to obtain the final product with high antiadhesive and antimicrobial activity.