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1.
J Musculoskelet Neuronal Interact ; 23(4): 426-435, 2023 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-38037361

RESUMO

OBJECTIVES: To examine effects of whole-body vibration (WBV) on bone properties in pre-type 2 diabetes mellitus (T2DM) rats. METHODS: Six-week-old male Hos:ZFDM-Lepr fa, fa/fa (DM) and Hos:ZFDM-Leprfa,fa/+ (CON; untreated non-DM) rats were used in the experiments. Half of DM rats were subjected to WBV (45 Hz, 0.5 g, 15 min/day, 5 days/week) for 8 weeks (WBV group), and the other half was not (DM group). RESULTS: Bone mass, trabecular bone microstructure (TBMS), and cortical bone geometry (CBG) parameters were worse in the DM and WBV groups compared with the CON group. Maximum load was significantly decreased in the DM group compared with the CON group, and the break point was significantly higher in the WBV group compared with the DM group. Serum levels of bone specific alkaline phosphatase were significantly lower in the WBV group compared with the CON group. Glycemic control was not worse in the WBV group compared with the DM group, but not the same levels as the CON group. CONCLUSIONS: These findings suggest that WBV can potentially delay the decrease in maximum load, although it does not prevent the deterioration of bone mass, TBMS, and CBG parameters.


Assuntos
Diabetes Mellitus Tipo 2 , Vibração , Ratos , Masculino , Animais , Vibração/uso terapêutico , Osso e Ossos/diagnóstico por imagem , Densidade Óssea , Osso Esponjoso
2.
Int Biomech ; 9(1): 19-26, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36384427

RESUMO

This study aimed to examine the continuous effects of whole body vibration (WBV) on bone properties, in growing rats. Fifty 5-week-old male rats were divided into control and experimental groups. Each experimental group underwent WBV at 50 Hz (0.5 g, 15 min/day, 5 days/week) for 5 or 10 weeks. Bone size, muscle weight and bone mechanical strength of the right tibia were measured. Trabecular bone microstructure, cortical bone geometry and bone mass of the left tibia were analyzed by micro-CT. Serum levels of bone formation/resorption markers were also measured. In rats that underwent 5-week WBV, tibial cortical bone mineral content and cortical bone area significantly increased (p < 0.05), and tibial cortical bone volume, thickness, maximum load, break point and stiffness tended to be increased (p = 0.05-0.09), compared with control rats. In rats that underwent 10-week WBV, stiffness tended to be increased (p = 0.07), and the serum level of osteocalcin decreased, compared with control rats. These findings suggest that 5-week WBV had beneficial effects on bone properties, and that increased bone mineral content and cortical bone geometry may lead to higher bone mechanical strength. Further studies will be needed to determine the optimal conditions of WBV for improving bone properties in the growth stage.


Assuntos
Osso e Ossos , Vibração , Ratos , Masculino , Animais , Vibração/uso terapêutico , Osso e Ossos/diagnóstico por imagem , Densidade Óssea/fisiologia , Osso Esponjoso/diagnóstico por imagem , Osso Cortical/diagnóstico por imagem
3.
J Musculoskelet Neuronal Interact ; 21(2): 287-297, 2021 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-34059574

RESUMO

OBJECTIVE: This study aimed to explore optimal conditions of whole-body vibration (WBV) for improving bone properties in aged rats. METHODS: Eighty-week-old rats were divided into baseline control (BC), age-matched control (CON) and experimental groups, which underwent WBV (0.5 g) at various frequencies (15, 30, 45, 60 or 90 Hz) or WBV (45 Hz) with various magnitudes (0.3, 0.5, 0.7 or 1.0 g) for 7 weeks. After interventions, femur bone size, bone mechanical strength and circulating bone formation/resorption markers were measured, and trabecular bone microstructure (TBMS) and cortical bone geometry (CBG) of femurs were analyzed by micro-CT. RESULTS: Several TBMS parameters and trabecular bone mineral content were significantly lower in the 15 Hz WBV (0.5 g) group than in the CON group, suggesting damage to trabecular bone. On the other hand, although frequency/magnitude of WBV did not influence any CBG parameters, the 0.7 g and 1.0 g WBV (45 Hz) group showed an increase in tissue mineral density of cortical bone compared with the BC and CON groups, suggesting the possibility of improving cortical bone properties. CONCLUSION: Based on these findings, it should be noted that WBV conditions are carefully considered when applied to elderly people.


Assuntos
Osso e Ossos , Vibração , Animais , Ratos , Densidade Óssea , Microtomografia por Raio-X
4.
Biomed Res ; 41(1): 13-22, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32092736

RESUMO

The number of patients with chronic obstructive pulmonary disease (COPD), a typical respiratory disorder, is rapidly increasing globally. The purpose of this study was to determine the effects of appropriate-intensity treadmill exercise on skeletal muscle and respiratory functions in a rat model of emphysema. Twenty-one Wistar rats were randomly divided into three groups: the sham (SH) group, pulmonary emphysema (PE) group, and emphysema + exercise (EX) group. Cigarette smoke solution and lipopolysaccharide were intratracheally administered for 4 weeks in the PE and EX groups. The rats in the EX group were made to run on treadmills in the latter 2 weeks of the experiment. Lung tissue was stained with anti-macrophage antibodies; the specific force (contractile force per unit cross-sectional area) of the diaphragm and hind-limb muscles was measured, and blood was analyzed for serum cytokine levels. Many macrophages were observed in the lung tissue of the PE group. In the EX group, the population of macrophages was smaller, and the specific force of the diaphragm and extensor digitorum longus muscles was higher than in the PE group. Moreover, the degree of inflammation in the pulmonary tissue was reduced in the EX group. These results suggest that adaptive exercise may improve not only respiratory and muscle functions but also inflammation of the pulmonary tissue associated with emphysema.


Assuntos
Músculo Esquelético/fisiopatologia , Condicionamento Físico Animal , Enfisema Pulmonar/fisiopatologia , Animais , Modelos Animais de Doenças , Inflamação , Lipopolissacarídeos , Pulmão/efeitos dos fármacos , Masculino , Contração Muscular , Ratos , Ratos Wistar , Respiração , Fumaça , Fumar/efeitos adversos
5.
Osteoporos Sarcopenia ; 5(3): 78-83, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31728424

RESUMO

OBJECTIVES: To examine the effects of whole body vibration (WBV) on bone properties in growing rats, and to explore the optimal conditions for enhancing bone properties. METHODS: Thirty-six 4-week-old male rats were divided into 1 control and 5 experimental groups. Each experimental group underwent WBV at 15, 30, 45, 60, and 90 Hz (0.5 g, 15 min/d, 5 d/wk) for 8 weeks. We measured bone size, muscle weight and bone mechanical strength of the right tibia. Trabecular bone mass and trabecular bone microstructure (TBMS) of the left tibia were analyzed by micro-computed tomography. Serum levels of bone formation/resorption markers were also measured. RESULTS: WBV at 45 Hz and 60 Hz tended to enhance trabecular bone mass and TBMS parameters. However, there was no difference in maximum load of tibias among all groups. Serum levels of bone resorption marker were significantly higher in the 45-Hz WBV group than in the control group. CONCLUSIONS: WBV at 45-60 Hz may offer a potent modality for increasing bone mass during the period of rapid growth. Further studies are needed to explore the optimal WBV conditions for increasing peak bone mass and TBMS parameters. WBV modality may be a potent strategy for primary prevention against osteoporosis.

6.
J Musculoskelet Neuronal Interact ; 19(2): 169-177, 2019 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-31186387

RESUMO

OBJECTIVE: This study aimed to examine whether WBV becomes a possible modality for the primary prevention of osteoporosis by exploring WBV frequency that has positive effects on bone properties in adult rats. METHODS: Thirty-six 24-week-old rats were divided into one control and 5 experimental groups, which underwent WBV at various frequencies (15, 30, 45, 60 or 90 Hz), with a magnitude of 0.5 g, for 15 min/day, 5 days/week, for 8 weeks. Bone size, muscle weight and bone mechanical strength were measured at the end of experimental period. Bone mass, trabecular bone microarchitecture (TBMA) and cortical bone geometry were analyzed by micro-CT. Circulating bone formation/resorption markers were determined by ELISA. RESULTS: Body weight-corrected soleus weight in all experimental groups and body weight-corrected extensor digitorum longus muscle weight in the 15 and 30 Hz groups were significantly higher than those of the control group, respectively. Femur trabecular thickness and width were significantly higher in the 15 Hz group than in the control group. However, there was no difference in bone mechanical strength or bone formation/resorption markers among all groups. CONCLUSION: These results suggest that WBV at low-frequencies may become a potent modality for the primary prevention of osteoporosis in adults.


Assuntos
Densidade Óssea/fisiologia , Osso Esponjoso/fisiologia , Vibração , Fatores Etários , Animais , Masculino , Ratos , Ratos Wistar , Vibração/uso terapêutico
7.
J Musculoskelet Neuronal Interact ; 18(4): 493-500, 2018 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-30511953

RESUMO

OBJECTIVE: We investigated the effects of upslope, level surface, and downslope running on indices of tibia and femur bone recovery in ovariectomized (OVX) rats. METHODS: Rats were randomly divided into five groups: one sham-operated (SHAM) group and four OVX groups. One OVX group was a non-running control (OVX-Cont) and the others performed upslope running (OVX-Up), level surface running (OVX-Level), or downslope running (OVX-Down) on a treadmill after ovariectomy. The metaphysis region of the proximal tibia, distal femur, and proximal femur were scanned by micro-computed tomography and various geometric and microarchitectural parameters as well as bone mineral density measured using bone analysis software. RESULTS: Tibial bone geometric parameters, BV/TV and trabecular thickness, were significantly improved in OVX-Up and OVX-Level groups compared to that in OVX-Cont and OVX-Down groups, and improved to a greater degree in OVX-Up group than in OVX-Level group. CONCLUSIONS: Therefore, running slope substantially influences the beneficial effects of treadmill running on OVX-induced bone degeneration, with upward running being more effective than level surface running or downslope running, likely due to the greater bone loads associated with upslope running. The benefits of upslope treadmill running were particularly observed in the proximal tibia.


Assuntos
Densidade Óssea/fisiologia , Doenças Ósseas Metabólicas/diagnóstico por imagem , Ovariectomia , Corrida/fisiologia , Tíbia/diagnóstico por imagem , Tíbia/fisiologia , Animais , Doenças Ósseas Metabólicas/reabilitação , Teste de Esforço/tendências , Feminino , Ovariectomia/efeitos adversos , Ovariectomia/tendências , Distribuição Aleatória , Ratos , Ratos Wistar , Corrida/tendências , Tomografia Computadorizada por Raios X/tendências
8.
Bone Rep ; 8: 18-24, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29322073

RESUMO

Intake of high-fat/high-sucrose (HFS) diet or high fat diet influences bone metabolism in young rodents, but its effects on bone properties of aged rodents still remain unclear. This study aimed to examine the effects of HFS diet intake on trabecular bone architecture (TBA) and cortical bone geometry (CBG) in aged rats. Fifteen male Wistar rats over 1 year were randomly divided into two groups. One group was fed a standard laboratory diet (SLD) and the other group was fed a HFS diet for six months. The femur/tibia, obtained from both groups at the end of experimental period, were scanned by micro-computed tomography for TBA/CBG analyses. Serum biochemical analyses were also conducted. Body weight was significantly higher in the HFS group than in the SLD group. In both femur and tibia, the HFS group showed higher trabecular/cortical bone mass in reference to bone mineral content, volume bone mineral density and TBA/CBG parameters compared with the SLD group. In addition, serum calcium, inorganic phosphorus, total protein, triacylglycerol, HDL and TRACP-5b levels were significantly higher in the HFS group than in the SLD group. There were good correlations between body weight and bone parameters in the femur and tibia. These results suggest that HFS diet intake results in higher bone mass in aged rats. Such effects of HFS diet intake might have been induced by increased body weight.

9.
Calcif Tissue Int ; 102(3): 358-367, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29103160

RESUMO

The purpose of this study was to examine the effects of green tea extract (GTE) intake on bone structural and physiological properties, such as bone mass, trabecular bone microarchitecture, cortical bone geometry, and bone mechanical strength, in growing rats. Four-week-old male Wistar rats were divided into the following four groups: standard diet feeding for 85 days (S-CON) or 170 days (L-CON), and GTE diet feeding for 85 days (S-GTE) or 170 days (L-GTE). At the end of the experiment, in addition to measurement of circulating bone formation/resorption markers, bone mass, trabecular bone microarchitecture, and cortical bone geometry were analyzed in the left femur, and bone mechanical strength of the right femur was measured. There was no difference in all bone parameters between the S-CON and S-GTE groups. On the other hand, the L-GTE group showed the decrease in some trabecular bone mass/microarchitecture parameters and no change in cortical bone mass/geometry parameters compared with the L-CON group, and consequently the reduction in bone weight corrected by body weight. There was no difference in bone formation/resorption markers and bone mechanical strength between the S-CON and S-GTE groups and also between the L-CON and L-GTE groups. However, serum leptin levels were significantly lower in the L-GTE group than in the L-CON group. Thus, the long-term GTE intake had negative effects on bone, especially trabecular bone loss and microarchitecture mal-conformation, in growing rats.


Assuntos
Densidade Óssea/efeitos dos fármacos , Osso e Ossos/efeitos dos fármacos , Fêmur/crescimento & desenvolvimento , Chá/efeitos adversos , Animais , Peso Corporal/efeitos dos fármacos , Peso Corporal/fisiologia , Densidade Óssea/fisiologia , Osso Cortical/crescimento & desenvolvimento , Fêmur/efeitos dos fármacos , Leptina/metabolismo , Masculino , Ratos , Ratos Wistar
10.
Data Brief ; 8: 1247-54, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27547805

RESUMO

This data article is related to the research article entitled, "Antioxidant potential in non-extractable fraction of dried persimmon (Diospyros kaki Thunb.)" (Matsumura et al., 2016) [1]. We investigated antioxidant activities of the non-extractable fraction of dried persimmon fruits in vitro and in vivo. We evaluated both extracted fraction and non-extractable fraction, and reported that non-extractable fraction may possess significantly antioxidant potential in vivo on the basis of the oxygen radical absorbance capacity (ORAC). We showed our experimental raw data about antioxidant capacity of dried persimmon, plasma triglycerides (TG) and HDL-cholesterol (HDL-C), and this data article might contribute to evaluate real antioxidant capacity of other fruits and vegetables.

11.
Food Chem ; 202: 99-103, 2016 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-26920271

RESUMO

Dried fruits of persimmon (Diospyros kaki Thunb.) are a traditional food in Japan and contain large quantities of tannins. In this study, we investigated the in vitro and in vivo antioxidant potentials of non-extractable fractions from dried persimmons. Hydrolysed non-extractable fractions showed the highest antioxidant activities in vitro. In subsequent experiments, the plasma oxygen radical absorbance capacity (ORAC) values in rats supplemented with a 5% non-extractable fraction were approximately 1.5times higher than those in control rats after 1week in vivo. Furthermore, using an in vitro model of the gastrointestinal tract, the ORAC values of the non-extractable fraction were significantly increased with colonic fermentation in the large bowel stage. These data indicate that non-extractable fractions may possess significant antioxidant potential in vitro and in vivo.


Assuntos
Antioxidantes/farmacologia , Diospyros , Extratos Vegetais/farmacologia , Animais , Frutas , Masculino , Ratos , Ratos Wistar
12.
BMC Pulm Med ; 15: 64, 2015 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-26059026

RESUMO

BACKGROUND: Systemic inflammation is present in chronic obstructive pulmonary disease (COPD). A whey peptide-based enteral diet reduce inflammation in patients with COPD, but its effect on COPD development has not been determined. On the other hand, it is known that short chain fatty acids (SCFAs), which are produced by micro-flora in the gut, attenuates bronchial asthma in mice model. METHODS: Mice with elastase-induced emphysema were fed with 1 of 3 diets (control diet, whey peptide-based enteral diet, or standard enteral diet) to determine the effects of whey peptide-based enteral diet on emphysema and on cecal SCFAs. RESULTS: The whey peptide-based enteral diet group exhibited fewer emphysematous changes; significantly lower total cell counts in bronchoalveolar lavage fluid (BALF); and significantly higher cecal SCFA levels than either the control or standard enteral diet groups. The total cell count was inversely correlated with total cecal SCFA levels in these three diet groups. CONCLUSIONS: The whey peptide-based enteral diet attenuates elastase-induced emphysema through the suppression of inflammation in the lung. This may be related to the increase in cecal SCFA.


Assuntos
Nutrição Enteral , Ácidos Graxos Voláteis/metabolismo , Interleucina-6/imunologia , Pulmão/efeitos dos fármacos , Enfisema Pulmonar/imunologia , Fator de Necrose Tumoral alfa/efeitos dos fármacos , Proteínas do Soro do Leite/farmacologia , Animais , Líquido da Lavagem Broncoalveolar/citologia , Caseínas/farmacologia , Ceco , Inflamação , Pulmão/imunologia , Pulmão/patologia , Camundongos , Camundongos Endogâmicos C57BL , Proteínas do Leite/farmacologia , Elastase Pancreática/toxicidade , Enfisema Pulmonar/induzido quimicamente , Enfisema Pulmonar/dietoterapia , Fator de Necrose Tumoral alfa/imunologia
13.
J Phys Ther Sci ; 27(3): 791-4, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25931732

RESUMO

[Purpose] The purpose of this study was to make an experimental model of cervical spinal cord injury (CSCI) using Wistar rats, in order to analyze the influence of CSCI on the respiratory function. [Subjects] Thirty-two male 12-week-old Wistar rats were used. [Methods] The CSCI was made at the levels from C3 to C7, and we performed pneumotachography and electromyography (EMG) on the diaphragm. Computed tomography was used to determine the level of spinal cord damage. [Results] After the operation, the tidal volume of the rats with a C3 level injury decreased to approximately 22.3% of its pre-injury value. In addition, in the same rats, the diaphragmatic electromyogram activity decreased remarkably. Compared with before CSCI, the tidal volume decreased to 78.6% of its pre-injury value in CSCI at the C5 level, and it decreased to 94.1% of its pre-injury value in CSCI at the C7 level. [Conclusion] In the rats that sustained a CSCI in this study, the group of respiratory muscles that receive innervation from the thoracic spinal cord was paralyzed. Therefore, the EMG signal of the diaphragm increased. These results demonstrate that there is a relationship between respiratory function and the level of CSCI.

14.
J Toxicol Sci ; 39(2): 331-7, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24646715

RESUMO

Cigarette smoke induces skeletal muscle wasting by a mechanism not yet fully elucidated. Branched-chain amino acids (BCAA) in the skeletal muscles are useful energy sources during exercise or systemic stresses. We investigated the relationship between skeletal muscle wasting caused by cigarette smoke and changes in BCAA levels in the plasma and skeletal muscles of rats. Furthermore, the effects of BCAA-rich diet on muscle wasting caused by cigarette smoke were also investigated. Wistar Kyoto (WKY) rats that were fed with a control or a BCAA-rich diet were exposed to cigarette smoke for four weeks. After the exposure, the skeletal muscle weight and BCAA levels in plasma and the skeletal muscles were measured. Cigarette smoke significantly decreased the skeletal muscle weight and BCAA levels in both plasma and skeletal muscles, while a BCAA-rich diet increased the skeletal muscle weight and BCAA levels in both plasma and skeletal muscles that had decreased by cigarette smoke exposure. In conclusion, skeletal muscle wasting caused by cigarette smoke was related to the decrease of BCAA levels in the skeletal muscles, while a BCAA-rich diet may improve cases of cigarette smoke-induced skeletal muscle wasting.


Assuntos
Aminoácidos de Cadeia Ramificada/administração & dosagem , Aminoácidos de Cadeia Ramificada/metabolismo , Dieta , Músculo Esquelético/metabolismo , Atrofia Muscular/dietoterapia , Atrofia Muscular/etiologia , Fumar/efeitos adversos , Aminoácidos de Cadeia Ramificada/sangue , Aminoácidos de Cadeia Ramificada/farmacologia , Animais , Metabolismo Energético , Masculino , Músculo Esquelético/patologia , Atrofia Muscular/metabolismo , Atrofia Muscular/patologia , Tamanho do Órgão/efeitos dos fármacos , Ratos , Ratos Endogâmicos WKY
15.
J Toxicol Sci ; 37(3): 575-85, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22687997

RESUMO

Dietary fiber, maintaining the gut environment, contributes to better lung function among smokers. This study was aimed to investigate the role of dietary fiber on the anti-oxidant capacity and gut environment during exposure to cigarette smoke. The anti-oxidant capacity as well as caecal levels of organic acids and population of micro-flora in the gut was measured after 4 months' exposure to cigarette smoke in mice (C57BL/6NcrSlc) fed with a cellulose-free diet. Animals were divided into control diet (AIN-93G)/non-smoke, cellulose-free diet/non-smoke, control diet/smoke, and cellulose-free diet/smoke groups. The anti-oxidant capacity in plasma was significantly suppressed by the cellulose-free diet in the non-smoke exposed mice. The suppression in the anti-oxidant capacity further declined following exposure to cigarette smoke. Both these changes in the anti-oxidant capacity were accompanied with changes in some organic acids levels in caecal contents. The anti-oxidant activity was significantly inversely correlated to succinic acid / acetic acid levels balance in caecal contents. In conclusion the cellulose-free diet suppressed the anti-oxidant capacity in mice, and the suppression further decreased by exposure to cigarette smoke. These changes in the anti-oxidant capacity may be related with changes in the gut environment.


Assuntos
Antioxidantes/metabolismo , Celulose/administração & dosagem , Dieta , Fibras na Dieta/administração & dosagem , Fumar/efeitos adversos , Acetatos/análise , Acetatos/metabolismo , Animais , Ceco/efeitos dos fármacos , Ceco/metabolismo , Ceco/microbiologia , Concentração de Íons de Hidrogênio , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Modelos Animais , Ácido Succínico/análise , Ácido Succínico/metabolismo
16.
J Toxicol Sci ; 37(1): 131-8, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22293417

RESUMO

Cigarettes smoke (CS) limits food intake and body weight increase. Ghrelin and leptin are hormones regulating appetite and energy balance. While ghrelin increases food intake and causes a positive energy balance, leptin decreases food intake and enhances a negative energy balance. To investigate the possible role of ghrelin and leptin regarding the negative energy balance caused by CS, 10-week old male Wistar rats (n = 10) were exposed to CS from 30 cigarettes twice a day for 5 days a week for four weeks. In the smoking group, food intake and body weight gain were less than those in the non-smoking group (n = 10) during the entire CS exposure. In the smoking group, the plasma levels of acyl ghrelin were significantly higher (75.9 ± 5.1 fmol/ml versus 46.5 ± 3.3 fmol/ml, p < 0.01), while those of leptin were significantly lower than those in the non-smoking group (434.9 ± 41.1 ng/ml versus 744.0 ± 45.4 ng/ml, p < 0.01) after the final CS exposure. However, the plasma des-acyl ghrelin levels were not affected by CS exposure. These results suggested that acyl ghrelin and leptin levels in plasma may change to compensate for the negative energy balance by CS.


Assuntos
Grelina/sangue , Leptina/sangue , Fumar/efeitos adversos , Animais , Carboxihemoglobina/análise , Ingestão de Alimentos/efeitos dos fármacos , Peróxido de Hidrogênio/sangue , Masculino , Ratos , Ratos Wistar , Aumento de Peso/efeitos dos fármacos
17.
J Toxicol Sci ; 36(3): 261-6, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21628954

RESUMO

Cigarette smoke has been known to affect the development of bowel disease. However it has not been fully elucidated how cigarette smoke has effects on the gut. In this context we evaluated not only caecal levels of organic acids but also populations of micro-flora and pH in caecal contents after exposing rats (n=5) to cigarette smoke for a 4-week in order to investigate whether the gut environment is altered by cigarette smoke or not. After the exposure of cigarette smoke, caecal levels of organic acids such as acetic acid, propionic acid, butyric acid and valeric acid significantly decreased. Additionally the population of Bifidobacterium significantly decreased and the pH significantly elevated. In conclusion cigarette smoke changes caecal levels of certain organic acids, the population of Bifidobacterium and the pH in caecal contents of rats. These results suggest that cigarette smoke may alter the gut environment of rats.


Assuntos
Ácidos Acíclicos/metabolismo , Bifidobacterium/efeitos dos fármacos , Ceco/efeitos dos fármacos , Ceco/microbiologia , Fumaça/efeitos adversos , Ácido Acético/metabolismo , Animais , Bifidobacterium/isolamento & purificação , Bifidobacterium/fisiologia , Peso Corporal/efeitos dos fármacos , Ácido Butírico/metabolismo , Ingestão de Alimentos/efeitos dos fármacos , Fezes/microbiologia , Concentração de Íons de Hidrogênio , Masculino , Ácidos Pentanoicos/metabolismo , Propionatos/metabolismo , Ratos , Ratos Wistar
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