Mouse model of microembolic stroke and reperfusion.

BACKGROUND AND PURPOSE: To test the role of fibrinolysis in stroke, we used a mouse model in which preformed 2.5- to 3-microm-diameter fibrin microemboli are injected into the cerebral circulation. The microemboli lodge in the downstream precapillary vasculature and are susceptible to fibrinolysis. METHODS: We injected various doses of microemboli into the internal carotid artery in mice and characterized their distribution, effects on cerebral blood flow, neurological deficit, infarct area, and spontaneous dissolution. By comparing wild-type and tissue plasminogen activator (tPA) knockout (tPA-/-) mice, we analyzed the role of endogenous tPA in acute thrombotic stroke. RESULTS: Microemboli cause dose-dependent brain injury. Although moderate doses of microemboli are followed by spontaneous reperfusion, they result in reproducible injury. Gene knockout of tPA markedly delays dissolution of cerebral emboli and restoration of blood flow and aggravates ischemic thrombotic infarction in the brain. CONCLUSIONS: We describe a microembolic model of stroke, in which degree of injury can be controlled by the dose of microemboli injected. Unlike vessel occlusion models, this model can be modulated to allow spontaneous fibrinolysis. Application to tPA-/- mice supports a key role of endogenous tPA in restoring cerebral blood flow and limiting infarct size after thrombosis.

Validation of the functional fitness age (FFA) index in older Japanese women.

Functional fitness age (FFA), which we previously described, is a measure of functional age that reflects a person's overall physical ability to complete daily tasks such as preparing meals and performing various household chores. The purpose of this study was to validate FFA in two elderly populations: 1) older subjects from different communities, and 2) older subjects participating in an exercise intervention program. FFA was calculated from 4 performance tests: performing arm curls; moving beans with chopsticks; demonstrating functional reach; and sitting and walking around two cones. The first study group was selected from 4 different communities (N=127), and was subdivided into a less active group (N=87) and an active group (N=40). The results of the 4 tests for the less active group were not significantly different from those for the original validation group used in developing the FFA equation. The FFA of the less active group (71.1+/-7.7 yrs) was not different from their chronological age (70.4+/-6.6 yrs). In the active group, except for the arm curls, test scores were significantly higher than those of the original validation group. The FFA of the active group (66.2+/-5.1 yrs) was significantly younger than their chronological age (70.9+/-4.2 yrs). Another group of 14 older women (79.5+/-3.9 yrs) was recruited for pre- and post-training testing. After the 3-month exercise program, they showed significant improvements in sitting and walking around two cones and functional reach; their FFA decreased significantly from 81.5+/-5.0 yrs to 78.3+/-4.5 yrs. The control group selected from the same community (N=16, 79.8+/-5.3 yrs) showed no significant changes in the 4 performance tests, and their FFA remained unchanged. These results indicate that our FFA is highly cross-validated for different Japanese communities, and is sensitive to changes associated with participation in an exercise intervention program.

Mental health issues affecting ethnic minorities in Japan.

Although Japan is becoming a multicultural society, the policy for ethnic minorities is not as mature as that in Western countries. Mental health issues among new ethnic minorities will be discussed. New minorities are mainly South-East Asian refugees, foreign brides from the Philippines, Korea and China, and returnees from China. Because their communities are very small, they tend to have great difficulties in coping with the Japanese society. Problems in their adjustment to the host country will be discussed.

Psychosomatic approach to patients with gynecologic cancer.

In patients with terminal cancer, there is a need for a psychosomatic approach in which both the patient's psychological state, including QOL, and physical condition are considered. We studied patients with gynecologic cancer with the following practical objectives: 1. To evaluate the effect of a psychosomatic approach to patients with terminal gynecologic cancer; 2. Using psychological tests and interviews, to clarify the character tendencies of cancer patients, which are often reported in other countries; and 3. To discuss the benefits in the patient's attitude toward living with cancer. In case studies, separation anxiety of terminal cancer patients is increased due to impending death, and often patients cannot deal with these feelings. When the medical staff accepts these emotions, the patients' feelings even in the face of death often change to positive feelings of gratitude to the people around them. All of the results of psychological testing showed model answers, close to the mean of the normal range. This suggests that the patients were suppressing emotions behind their standard responses. Patients with uterine cancer showed a type C or cancer character, while those with ovarian cancer showed type A or heart disease character. Results indicated different attitudes between patients with uterine cancer and those with ovarian cancer. Cancer patients with a vigorous attitude (high POMS-V score pattern behavior) tended to have a good prognosis. This investigation represents a pilot study of a psychosomatic approach to cancer patients. However, recent psychoneuroimmunologic studies have reported the influence of emotion on cancer. Further studies of this kind are needed.

Family factors affecting adjustment in Japanese immigrant housewives.

Data obtained from 130 questionnaires distributed to immigrant Japanese housewives were analyzed to determine if family factors were associated with poor adjustment, and in particular with depressive symptoms. It was possible to identify women who had no major adjustment problems in adapting to life in Canada (n = 70), transitional adjustment problems (n = 30), late onset adjustment problems (n = 7) and chronic adjustment problems (n = 23). These categories were determined by self-report and validated by symptom identification. Style of marriage, communication with spouse and mother-child interactions were hypothesized to be related to adjustment problems. A 16 item questionnaire was developed to assess the degree to which these factors affected adjustment. The only factor which affected degree of adjustment was communication with the spouse. These results are discussed within the context of traditional hypotheses concerning adaptation to a new culture and the implication for treatment.

Structure of Cell Wall and Extracellular Mannans from Saccharomyces rouxii and Their Relationship to a High Concentration of NaCl in the Growth Medium.

Cells of Saccharomyces rouxii (a salt-tolerant yeast) were grown in the presence of two levels of NaCl, 0 and 15%. Mannans obtained from both the cell walls and culture filtrates (extracellular) were examined. Yields based on the dry weight of cells demonstrated that the levels of both cell wall and extracellular mannans were lower when cells were grown in the presence of 15% NaCl. However, the carbohydrate and protein contents of the mannan preparations were not altered. The cell wall mannans obtained from the two growth conditions had similar molecular weights, whereas the extracellular mannans had different molecular weight distributions. Structural analyses showed that the cell wall and extracellular mannans had similar structures. Both had an alpha1-6-linked backbone to which single mannose and mannobiose units were connected as side chains, predominantly by alpha1-2 linkages. The mannans also contained mannosyloligosaccharides, mannotriose, mannobiose, and mannose attached to protein through an O-glycosidic bond. The molecular structure of the cell wall mannans remained unchanged at both levels of NaCl. However, in the presence of 15% NaCl, the side chains consisting of a mannobiose unit were slightly reduced.

Influence of pH on Inhibitory Activity of Acetic Acid on Osmophilic Yeasts Used in Brine Fermentation of Soy Sauce.

The inhibitory activity of acetic acid on osmophilic yeasts (Saccharomyces rouxii and Torulopsis versatilis) used for the brine fermentation of soy sauce increased remarkably as the pH of the medium was lowered. This increased toxicity could not be attributed to a hydrogen ion effect alone.

Antagonism between osmophilic lactic Acid bacteria and yeasts in brine fermentation of soy sauce.

Brine fermentation by osmophilic lactic acid bacteria and yeasts for long periods of time is essential to produce a good quality of shoyu (Japanese fermented soy sauce). It is well known that lactic acid fermentation by osmophilic lactic acid bacteria results in the depression of alcoholic fermentation by osmophilic yeasts, but the nature of the interaction between osmophilic lactic acid bacteria and yeasts in brine fermentation of shoyu has not been revealed. The inhibitory effect of osmophilic lactic acid bacteria on the growth of osmophilic yeasts was investigated. It was recognized that osmophilic shoyu yeasts such as Saccharomyces rouxii and Torulopsis versatilis were inhibited by a metabolite produced by osmophilic lactic acid bacteria (belonging to Pediococcus halophilus) in brine fermentation of shoyu. The primary inhibitor was considered to be acetic acid, although lactic acid was slightly inhibitory.