RESUMO
In this study, internalization of positively charged chitosan-coated nanoparticles (198AuNPs@chitosan) on MCF-7 cells was investigated by γ-ray spectroscopy and then statistically compared to that of 198Au and negatively charged citrate-stabilized nanoparticles (198AuNPs). Sub-50â¯nm 198AuNPs@chitosan had a higher internalization compared to 198Au and 198AuNPs (pâ¯<â¯0.05). More cellular uptake of 198AuNP@chitosan means a higher dose of radioactivity to the tumor cells which, in turn, more effective treatment of the cancer.
Assuntos
Quitosana/administração & dosagem , Radioisótopos de Ouro/administração & dosagem , Radioisótopos de Ouro/farmacocinética , Nanopartículas Metálicas/administração & dosagem , Compostos Radiofarmacêuticos/administração & dosagem , Compostos Radiofarmacêuticos/farmacocinética , Transporte Biológico Ativo , Neoplasias da Mama/metabolismo , Neoplasias da Mama/radioterapia , Quitosana/química , Materiais Revestidos Biocompatíveis/administração & dosagem , Materiais Revestidos Biocompatíveis/química , Sistemas de Liberação de Medicamentos , Endocitose , Exocitose , Feminino , Radioisótopos de Ouro/química , Humanos , Células MCF-7 , Nanopartículas Metálicas/química , Nanotecnologia , Tamanho da Partícula , Compostos Radiofarmacêuticos/química , Espectrometria de Fluorescência , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de SuperfícieRESUMO
The 63Zn was produced by16.8â¯MeV proton irradiation of natural copper. Thick target yield for 63Zn in the energy range of 16.8 â12.2â¯MeV was 2.47 ± 0.12 GBq/µA.h. Reasonable agreement between achieved experimental data and theoretical value of thick target yield for 63Zn was observed. A simple separation procedure of 63Zn from copper target was developed using cation exchange chromatography. About 88 ± 5% of the loaded activity was recovered. The performance of FLUKA to reproduce experimental data of thick target yield of 63Zn is validated. The achieved results from this code were compared with the corresponding experimental data. This comparison demonstrated that FLUKA provides a suitable tool for the simulation of radionuclide production using proton irradiation.