RESUMO
Four levels (types) of immune response, differing by expression of cytokines (TNF-alpha, IL-1 beta, IL-4, and gamma-IFN) and immunoglobulins IgG2, IgG3, IgG4, and IgE) and by expression and time course of specific cell-mediated and humoral immune response, were detected in children with different clinical forms of mumps. Types 1 and 3 immune response are predominantly cell-mediated, while types 2 and 4 predominantly humoral during the acute phase of the disease. The cytokine and antigen-specific profiles of each type of immune response correlate with the severity of clinical course of mumps.
Assuntos
Anticorpos Antivirais/biossíntese , Imunidade Celular , Caxumba/imunologia , Criança , Citocinas/biossíntese , Humanos , Imunoglobulinas/biossíntese , Imunoglobulinas/classificação , Caxumba/patologia , Vírus da Caxumba/imunologiaRESUMO
The immunological study of children with infectious parotitis (IP) without complications and with such complications as pancreatitis, meningitis or orchitis in the glandular form was carried out. In accordance with the previously proposed principle, 4 types of immune response (IR) were established on the basis of differences in initial resistance and the IR profile: cell-mediated immunity (types I and III) and humoral immunity (types II and IV). The patients included nonvaccinated children, as well as children vaccinated on epidemic indications, 3-6, 7-9, 10 and more years before infection. The comparative analysis of the number of IP cases with and without complications in the groups of children, divided according to their immunization history and the type of IR, revealed that postvaccinal immunity in children vaccinated on epidemic indications (less than a month ago) or 3-6 years before infection had protective potential, sufficient for the prevention of complicated forms of IP. Immunity obtained 7-9 years ago was effective for the protection from IP complications only in cell-mediated, but not humoral IR. Postvaccinal immunity obtained more than 10 years ago did not ensure the decrease in the occurrence of complicated forms of IP (in comparison with that in nonvaccinated patients) in children with any type of IR.
Assuntos
Vacina contra Caxumba/imunologia , Caxumba/prevenção & controle , Adolescente , Anticorpos Antivirais/sangue , Formação de Anticorpos , Criança , Pré-Escolar , Humanos , Imunidade Celular , Masculino , Caxumba/complicações , Caxumba/imunologia , Vírus da Caxumba/imunologia , Linfócitos T/imunologia , Fatores de TempoRESUMO
Virus adsorption on cell receptors changed the state of cellular membranes, as was shown on a model of 25 viruses belonging to 13 families. This is indicated by an increase in the fluorescence of the DSM probe. Homologous antibodies block the virus adsorption on the cell membrane, this permitting identification of viruses and detection of antibodies to them.