Effect of increasing dietary protein with constant lysine:methionine ratio on production and omasal flow of nonammonia nitrogen in lactating dairy cows.

Eight lactating cows were fed 4 diets in which dietary crude protein (CP) was increased in steps of approximately 2 percentage units from 11 to 17% of DM by replacing high-moisture corn with soybean meal supplemented with rumen-protected Met to maintain a Lys:Met ratio of 3:1 in metabolizable protein. Trial design was a replicated 4 × 4 Latin square; experimental periods lasted 28 d, with data and sample collection being performed during wk 3 and 4 of each period. Digesta samples were collected from the rumen as well as the omasum to measure metabolite concentrations and ruminal outflow of N fractions using infusion of 15N-enriched ammonia to quantify microbial nonammonia N (NAN) and nonmicrobial NAN. Data were analyzed using the MIXED procedure of SAS (SAS Institute Inc.). There were linear increases in the yields of milk and true protein and concentration of milk urea N, and a linear decrease in N efficiency, with increasing dietary CP. Apparent ruminal and total-tract N digestibility increased linearly with increasing dietary CP, but estimated true total-tract N digestibility was not affected. Apparent digestibility of the other macronutrients was not influenced by diet. Ruminal ammonia, total AA and peptides, and branched-chain VFA also increased linearly with dietary CP. The 15N enrichment of liquid- and particle-associated microbes linearly declined with increasing dietary CP due to decreasing 15N enrichment of the ammonia pool. Although no effect of dietary CP on nonmicrobial NAN flow was detected, total NAN flow increased linearly from 525 g/d at 11% CP to 637 g/d at 17% CP due to the linear increase in microbial NAN flow from 406 g/d at 11% CP to 482 g/d at 17% CP. Under the conditions of this study, when dietary CP was increased by adding soybean meal supplemented with rumen-protected Met, improved milk and protein yields were driven not by RUP supply but by increased ruminal outflow of microbial protein.

Lactation response to soybean meal and rumen-protected methionine supplementation of corn silage-based diets.

Corn silage, an important forage fed to dairy cows in the United States, is energy rich but protein poor. The objectives of this experiment were to investigate the effects on production of milk and milk components of feeding corn silage-based diets with 4 levels of dietary crude protein (CP) plus rumen-protected methionine (RPM). Thirty-six cows were blocked by days in milk into 9 squares and randomly assigned to 9 balanced 4 × 4 Latin squares with four 4-wk periods. All diets were formulated to contain, as a percent of dry matter (DM), 50% corn silage, 10% alfalfa silage, 4% soyhulls, 2.4% mineral-vitamin supplement, and 30% neutral detergent fiber. Supplemental RPM (Mepron, Evonik Corp., Kennesaw, GA) was added to all diets to maintain a Lys:Met ratio of 3.1 in digested AA. Ground high-moisture corn was reduced and soybean meal (SBM) plus RPM increased to give diets containing, on average, 11% CP (28% corn, 31% starch, 6% SBM, 4 g of RPM/d), 13% CP (23% corn, 29% starch, 10% SBM, 8 g of RPM/d), 15% CP (19% corn, 26% starch, 15% SBM, 10 g of RPM/d), and 17% CP (14% corn, 24% starch, 19% SBM, 12 g of RPM/d). Data from the last 14 d of each period were analyzed using the mixed procedures in SAS (SAS Institute Inc., Cary, NC). With the exception of milk fat and milk lactose content, we found no significant effects of diet on all production traits. We did note linear responses to dietary CP concentration for intake, production of milk and milk components, and MUN. Cows fed the 11% CP diet had reduced DM intake, lost weight, and yielded less milk and milk components. Mean separation indicated that only true protein yield was lower on 13% CP than on 17% dietary CP, but not different between 15 and 17% CP. This indicated no improvement in production of milk and milk components above 15% CP. Quadratic trends for yield of milk, energy-corrected milk, and true protein suggested that a dietary CP concentration greater than 15% may be necessary to maximize production or, alternately, that a plateau was reached and no further CP was required. Although diet influenced apparent digestibility of DM, organic matter, and neutral detergent fiber, digestibility did not increase linearly with dietary CP. However, we observed linear and quadratic effects of dietary CP on acid detergent fiber digestibility. As expected, we found a linear effect of dietary CP on apparent N digestibility and on fecal and urinary N excretion, but no effect of diet on estimated true N digestibility. Ruminal concentrations of ammonia, total AA, peptides, and branched-chain volatile fatty acids also increased linearly with dietary CP. Quadratic responses indicated that 14.0 to 14.8% CP was necessary to optimize digestion and energy utilization. Overall results indicated that, when RPM was added to increase Lys:Met to 3.1, 15% CP was adequate for lactating dairy cows fed corn silage diets supplemented with SBM and secreting about 40 kg of milk/d; N excretion was lower than at 17% CP but with no reduction in yield of milk and milk components.