RA-dimer†B, a New Dimeric RA-series Cyclopeptide Incorporating Two Different Types of Cycloisodityrosine Units, from Rubia cordifolia†L.

RA-dimer B, a new cytotoxic RA-series peptide, was isolated from the roots of Rubia cordifolia L. Its structure was elucidated on the basis of spectroscopic analysis to be a dimeric cyclopeptide composed of deoxybouvardin and allo-RA-V. Those two cyclopeptide units are connected by an ether linkage between the phenolic oxygen atom of deoxybouvardin and the ϵa carbon atom of Tyr-6 of allo-RA-V. RA-dimer B was synthesized by the coupling reaction of deoxybouvardin with the boronic acid derivative of allo-RA-V, and subsequent deprotection, confirming the relative stereochemistry and establishing the absolute configuration of this peptide. RA-dimer B showed cytotoxic activity against human promyelocytic leukaemia HL-60, human colonic carcinoma HCT-116, and human renal cell carcinoma ACHN cells with IC50 values of 0.59, 0.54, and 0.74 µm, respectively.

[Living areas considered by healthy elderly people living in the suburbs of a metropolitan area].

PURPOSE: The purpose of this study was to identify living areas perceived by healthy elderly people living in A, a Metropolitan city. The study also focused on the influences of age, gender, and family structures, residence period, the level of instrumental activities of daily living (the IADL), and frequency of weekly outing of the elderly upon their perceptions. METHODS: The inclusion criteria for the study subjects were: (1) elderly people aged 65 and over living in A city, and (2) not receiving Long-Term Insurance services. The survey period was between January and February in 2005. Self-administered questionnaires were sent to 4,000 randomly selected elderly people by mail and 3,070 (77.0%) questionnaires were returned. 2,692 (67.3%) of these could be analyzed. RESULTS: The majority of respondents perceived the entire area of A city and the local center's area as living areas. Junior high school districts and primary school districts were stated by less than 3% of all respondents as within their living areas. Regarding gender, men were more likely to respond that the local center's area was their living area, whereas women considered that the whole area of A city was included. However, there was no statistically significant difference. In terms of age, respondents aged under 79 included the whole area of A city, whereas those aged 80 and under stated that their areas of self-governing body/neighborhood association were their living areas, indicating reduction in living areas with aging. Regarding the family structure of the respondents, the most frequent answer was the entire K city in subjects in all types of households except those consisting of the subject and parents, who most frequently regarded local center's as the living area. Respondents who were living 10 years and over in K city perceived that the whole area of the city was included, but respondents residing for under 10 years perceived the local center's area to be their living area. With regard to the level of the IADL, independent elderly responded the entire city, whereas dependent elderly responded the local center's area. Moreover, the living areas of respondents became smaller with decrease in the frequency of weekly outing. CONCLUSION: The living areas perceived by elderly people differ depending on their attributes, including age and gender, IADL, frequency of weekly outing, and the period of residence. In order to provide support to facilitate the elderly staying at home, we need to consider their backgrounds and thoughts. Further investigation is necessary to identify the influences of the characteristics and environments of communities upon the elderly perception of living areas.