RESUMO
Bioactive compound substances from Grifola frondosa (maitake) inhibited adipocyte differentiation of B(2)C(1) preadipocytes. This compound is not related to MAP kinase (ERK1 and ERK2) cascades or beta-catenin, which inhibit the expression of Glut4, PPARgamma and C/EBPalpha. The compound reduced the expression time of C/EBPbeta and C/EBPdelta. These results suggest that the inhibitory action of these bioactive compounds on adipocyte differentiation is exhibited through preadipocytes. They cannot induce the expression of PPARgamma or C/EBPalpha because of the reduced expression time of C/EBPbeta and C/EBPdelta.
Assuntos
Adipócitos/metabolismo , Proteína beta Intensificadora de Ligação a CCAAT/efeitos dos fármacos , Proteína delta de Ligação ao Facilitador CCAAT/efeitos dos fármacos , Grifola , Extratos Vegetais/farmacologia , Animais , Western Blotting , Proteína beta Intensificadora de Ligação a CCAAT/genética , Proteína beta Intensificadora de Ligação a CCAAT/metabolismo , Proteína delta de Ligação ao Facilitador CCAAT/genética , Proteína delta de Ligação ao Facilitador CCAAT/metabolismo , Técnicas de Cultura de Células , Diferenciação Celular/genética , Eletroforese em Gel de Poliacrilamida , Regulação da Expressão Gênica/genética , Grifola/química , Camundongos , Extratos Vegetais/isolamento & purificação , CoelhosRESUMO
We investigated the effect of a water-soluble extract from Grifola frondosa, the maitake mushroom, on lipid droplets in brown adipocyte tissue (BAT) cells. This water-soluble extract inhibits the conversion of pre white adipocyte tissue (WAT) cells but does not inhibit that of pre BAT cells. It reduces the amount of accumulated triglycerides (TG) in BAT cells. The glycerol-3-phosphate dehydrogenase (GPDH) activities of BAT cells decreased, but the expression of uncoupling protein 1 (UCP1) levels increased. These results suggest that maitake extract inhibits TG accumulation-related energy metabolism.