Resistance of mosquitoes to Lambda-Cyhalothrin and DDT in a Niger Delta Region of Nigeria.

Background: The escalation of insecticide resistance across the World Health Organization (WHO) African region calls for the routine monitoring of insecticide resistance in mosquitoes. While pyrethroids are recommended for use and dichlorodiphenyltrichloroethane (DDT) has been prescribed, it is imperative to reevaluate their efficacy across diverse geographical settings. The extent of resistance among mosquitoes to DDT and lambda-cyhalothrin remains understudied in Ika North East, Delta State, Nigeria, where the yellow fever virus was reported in 2019. Materials and Methods: We investigated the susceptibility of female mosquitoes to DDT (4%) and lambda-cyhalothrin (0.05%) in this specific study area. Adult female mosquitoes of Anopheles gambiae, Culex quinquefasciatus, and Aedes albopictus, aged between 2 and 4 days, were subjected to these insecticides utilizing the WHO bioassay method. The assessment of knockdown was done between 10 and 60 min, then mortality after 24 h. Results: C. quinquefasciatus and A. albopictus mosquitoes exposed to DDT and lambda-cyhalothrin exhibited high susceptibility, resulting in complete mortality (100%); however, A. gambiae displayed resistance, with mortality rates of 19% and 76%, respectively . Notably, A. gambiae mosquitoes exposed to piperonyl butoxide (PBO)-DDT and PBO-lambda-cyhalothrin exhibited enhanced mortality, reaching 95% (indicating suspected resistance) and 100% (indicating susceptibility), respectively. Knockdown time (KDT) for 50% in mosquitoes exposed to lambda-cyhalothrin ranged from 3.94 to 33.51 min. Similarly, KDT model for 95% ranged from 19.04 to 84.15 min. Among the tested mosquito species, Culex mosquitoes exhibited the shortest knockdown resistance time for lambda-cyhalothrin, recorded at 3.94 min. Similarly, the KDT for DDT ranged from 24.97 to 187.06 min for 50% mortality and from 61.04 to 431.03 min for 95% mortality, respectively. Anopheles mosquitoes exposed to PBO + DDT recorded the lowest KDT. Conclusion: Our study underscores the potential of exercising caution in the use of DDT and lambda-cyhalothrin insecticides for the control of mosquitoes due to emerging resistance.

Susceptibility status and synergistic activity of DDT and Lambda-cyhalothrin on Anopheles gambiae and Aedes aegypti in Delta State, Nigeria.

The detection of insecticide resistance in male mosquitoes has been treated with less importance in monitoring insecticide resistance spread in mosquitoes. There are no studies on the susceptibility and synergistic activity of DDT and lambda-cyhalothrin on male Anopheles gambiae and Aedes aegypti in Delta State, Nigeria. Even though studies have extensively reported resistance in female mosquitoes, the susceptibility of male mosquitoes to insecticide classes should be ascertained. In this study, we tested the susceptibility status and synergistic activity of DDT and Lambda-cyhalothrin on An. gambiae and Ae. aegypti in Delta State, Nigeria, in order to ascertain the level of resistance and knockdown. In addition, we modelled the knockdown time using Probit analysis model. WHO bioassay method was used to expose two days old adult mosquitoes to 4% DDT and 0.05% lambda-cyhalothrin. The results showed that An. gambiae mosquitoes exposed to DDT and lambda-cyhalothrin were confirmed resistant (61% and 53% respectively). However, pre-exposing the resistant mosquito population to piperonyl butoxide (4%) showed an increase in mortality to 90% (possible resistance) in DDT and 98% (susceptible) in lambda-cyhalothrin. Ae. aegypti mosquitoes exposed to DDT were susceptible (98%) while those exposed to lambda-cyhalothrin were confirmed resistant (87%) and this increased to complete mortality (100%) in PBO+lambda-cyhalothrin population. Furthermore, the results showed that the knockdown time (KDT50 and KDT95) in An. gambiae exposed to DDT was 39.5-71.2 minutes and 124.5-146.4 minutes respectively, while that of lambda-cyhalothrin was 33.0-81.8 minutes and 64.0-124.4 minutes respectively. In Ae. aegypti, KDT50 and KDT95 was 23.9 and 61.7minutes for DDT exposure whereas it was 5.6-15.3 minutes and 36.1-72.3 minutes for lambda-cyhalothrin exposure. It can be concluded that male An. gambiae mosquitoes exposed to the insecticides were resistant and the causes may be linked to certain resistant genes in the mosquitoes. The chances of transferring resistance are possible in wild species and molecular-based studies on the resistant gene in male mosquitoes as well as the tendencies of transfer are required to establish this focus.

Insecticidal effectiveness of naphthalene and its combination with kerosene against the emergence of Aedes aegypti in Ika North East, LGA, Delta State, Nigeria.

Despite the substantial progress achieved in the search of nonchemical alternatives to insecticidal larviciding on mosquitoes, more work is still required to unravel the potency of viable substances in order to attend to several pest and disease problems. Insecticidal effectiveness of naphthalene and its combination with kerosene against the emergence of Ae. aegypti in Ika North East, LGA, Delta State, Nigeria was assessed. Immature stages of Ae. aegypti were collected and left to acclimatize for 6 h in standard laboratory conditions. Naphthalene measured in 2 g and its combinations with kerosene in 50:50 were emptied in 400 ml, 200 ml and 100 ml of water which resulted in 0.005%, 0.01% and 0.02% concentrations respectively. Water alone served as control for the experiment. Twenty third instar larvae and pupae were sorted into containers before exposure to treatments. Experiment was done in triplicates and observed for 10, 15, 20, 30, 40, 50, 60, and 80 min coinciding with WHO protocol for Aedes exposure. Mortality was highest in larvae exposed to 0.02% kerosene and naphthalene, and was also high in 0.02% naphthalene. Lowest mortality was recorded in pupae exposed to 0.005% of naphthalene. Significant differences in toxicity was recorded (p < 0.05). Mortality increased with time in larvae and pupae. Highest mortality in pupae and larvae was recorded in 0.02% kerosene and naphthalene mixture at 80 min post exposure time respectively. LC50 and LC95 of naphthalene exposed to Aedes larvae and pupae was between 0.002 and 0.018% and 0.021-0.051% respectively. Similarly, for naphthalene with kerosene was between 0.002 and 0.007%, and 0.015-0.035%. Pupae exposed to 0.005% naphthalene had more adult emergence than in others and the differences were significant (p < 0.05). Field trial is required with optimum concentrations.

Pyrethroid Susceptibility in Culex quinquefasciatus Say. (Diptera: Culicidae) Populations from Delta State, Niger-Delta Region, Nigeria.

The development of insecticide resistance in different species of mosquitoes to Pyrethroids is a major challenge for vector-borne diseases transmitted by mosquitoes. Failure of Pyrethroids in control of mosquitoes would impact negatively on the gains recorded in control of mosquito-borne diseases in previous years. In anticipation of a country-wide deployment of Pyrethroid-treated nets for control of mosquito-borne diseases in Nigeria, this study assessed susceptibility of Culex quinquefasciatus Say. (Diptera: Culicidae) to Pyrethroids in Owhelogbo, Ejeme and Oria-Abraka communities in Delta State, Niger-Delta, Nigeria. Three to five day old Cx. quinquefasciatus were exposed to Deltamethrin (0.05%), Permethrin (0.75%), and Alphacypermethrin (0.05%) using World Health Organization bioassay method. Polymerase chain reaction (PCR) was employed in characterization of species and knockdown mutation. Results revealed that Cx. quinquefasciatus were generally susceptible (98-100%) to Deltamethrin, Permethrin, and Alphacypermethrin in the three communities with the exception of Owhelogbo where resistance to Deltamethrin (97%) was suspected. Knockdown time to Deltamethrin (11.51, 11.23, and 12.68 min), Permethrin (28.75, 13.26, and 14.49 min), and Alphacypermethrin (15.07, 12.50, and 13.03 min) were considerably low for Owhelogbo, Ejeme, and Oria-Abraka Cx. quinquefasciatus populations, respectively. Species identification result showed that all amplified samples were Cx. quinquefasciatus; however, no kdr allele was found in the three populations. Deployment of pyrethroid-treated nets for control of mosquito-borne diseases in Niger-Delta region of Nigeria is capable of reducing burden of diseases transmitted by Cx. quinquefasciatus as well as addressing nuisance value of the vector; however, caution must be entertained so as not to increase selection pressure thereby aiding resistance development.