Ecotoxicological effects of leachate from e-cigarettes and e-liquid on the performance of perennial ryegrass (Loliumperenne).

Once littered, disposable e-cigarettes present a complex type of waste in the environment. They typically contain a lithium battery, electronics to produce vapour and remnant e-liquid, all of which could leach into the environment. The effects of littered e-cigarettes are not well understood, and they have not been tested in terrestrial ecosystems. To address this, an experiment was set up to assess how leachate from e-cigarettes with or without a battery, but also e-liquid on its own can alter fundamental physical characteristics of Lolium perenne (perennial ryegrass) when irrigated with contaminated water. After 31 days, shoot length of L. perenne was not measurably affected, but the biomass was significantly reduced by 30% when e-liquid, and 24% when leachate from intact e-cigarettes was present compared to control plants. Plants grown with leachate or e-liquid displayed a significant level of early senescence of leaf apices, and the chlorophyll content was increased. Furthermore, root biomass was significantly less (29-46%) compared to the control. Leachate from used disposable e-cigarettes can affect the performance of plants when entering the soil ecosystem, therefore stricter regulations are needed to prevent this new type of electronic litter from becoming more widespread.

Physical and chemical effects of conventional microplastic glitter versus alternative glitter particles on a freshwater plant (Lemnaceae: Lemna minor).

Glitters are primary microplastics which are directly littered into the environment, yet the ecological effects have seldom been tested. When microplastics enter the environment, their physical presence and chemical leachate may alter the physiology of primary producers. Glitter can be composed of plastic or natural and/or biodegradable materials, often with additives. Three experiments were run for 14 days to separate chemical and physical effects of different types of glitter: polyethylene terephthalate (PET), biodegradable modified regenerated cellulose (MRC), synthetic mica, and a natural particle control (kaolinite) on several physical characteristics of Lemna minor (common duckweed). L. minor was exposed to either fresh (chemical and physical effects), leachate from glitter (chemical) or aged glitter (physical). Overall, there was little effect of PET, synthetic mica, kaolinite or of any aged glitter. High concentrations of fresh MRC glitters, however, decreased root length, biomass and chlorophyll content of L. minor. Some of these effects were also present when exposed to leachate from MRC glitters, but were less pronounced. Elemental analysis revealed the presence of metals in MRC glitters which may explain these responses. Short-term ecotoxicity of biodegradable glitters can arise due to their physical and chemical properties, but may lessen over time as their surface coating degrades.

Disposable e-cigarettes and cigarette butts alter the physiology of an aquatic plant Lemna minor (Lemnaceae).

Cigarette butts are one of the most littered single-use plastic items worldwide and can have ecotoxicological effects. e-Cigarettes (or 'vapes') have gained popularity globally as an alternative to tobacco-based cigarettes in recent years. The increased availability of disposable e-cigarettes has resulted in a rise in their littering, presenting a complex form of e-waste in the environment, yet the environmental impacts of littered e-cigarettes are largely unknown. Here Lemna minor (common duckweed) was used as a model organism to understand the effects of smoked cigarettes, vaped e-cigarettes or e-liquid. Growth-related physiological responses were measured (biomass, root development and frond chlorophyll content). After 14 days of exposure to cigarette butts there was an increase of all measured growth variables. On the contrary, exposure to e-cigarettes or e-liquid caused a decrease in root length, biomass and frond number relative to controls. The significant deviations from control conditions suggest that the presence of either cigarette butts, e-cigarettes or e-liquid caused stress to the model plant which could indicate disruption of aquatic ecosystems at the primary producer level. Disposable vapes pose a novel threat to aquatic ecosystems and it would be prudent to prevent them from becoming the next top litter item.