Effect of corn dry distiller grains plus solubles supplementation level on performance and digestion characteristics of steers grazing native range during forage growing season.

Two experiments were conducted to evaluate effects of corn dry distiller grains plus condensed solubles (DDGS) supplementation level on performance digestion characteristics of steers grazing native range during the forage growing season. In the performance study, 72 (206 ± 23.6 kg; 2008) and 60 (230 ± 11.3 kg; 2009) English crossbred steer calves were used in a randomized complete block design replicated over 2 yr. The grazing periods lasted 56 and 58 d and started on August 11 and 18 for 2008 and 2009, respectively. Each year, steers were blocked by BW (light, medium, and heavy), stratified by BW within blocks, and randomly assigned to 1 of 4 grazing groups. Each grazing group (6 steers in 2008 and 5 in 2009) was assigned to a DDGS supplementation levels (0, 0.2, 0.4, and 0.6% BW). Grazing group served as the experimental unit with 12 groups per year receiving 1 of 4 treatments for 2 yr (n = 6). In the metabolism study, 16 English crossbred steers (360 ± 28.9 kg) fitted with ruminal cannulas grazing native range during the summer growing season were used in a completely randomized design to evaluate treatment effects on forage intake and digestion. The experiment was conducted during the first and second weeks of October 2008. Steers were randomly assigned to supplement level (0, 0.2, 0.4, and 0.6% BW; n = 4) and grazed a single native range pasture with supplements offered individually once daily at 0700 h. In the performance study, ADG (0.64, 0.75, 0.80, and 0.86 ± 0.03 kg/d for 0, 0.2, 0.4, and 0.6% BW, respectively) increased linearly (P = 0.01) with increasing DDGS supplementation level. In the metabolism study, forage OM, NDF, CP, and ether extract (EE) intake decreased (P ≤ 0.05) linearly with increasing DDGS supplementation level. Total CP and EE intake increased (P ≤ 0.002) with increasing DDGS supplementation level. Digestibility of OM, NDF, and EE increased (linear; P ≤ 0.008) whereas the soluble CP fraction of forage masticate sample linearly increased (P = 0.01) and slowly degradable CP fraction linearly decreased (P = 0.05) with increasing DDGS supplementation level. Forage in situ masticate DM and NDF disappearance rate decreased (quadratic; P ≤ 0.05) and DDGS in situ DM disappearance rate increased (linear; P = 0.03) with increasing supplementation levels. These results indicate that DDGS supplementation enhanced grazing performance and total-tract digestion of steers grazing native range during the forage growing season.

Isolation of viable porcine islets by selective osmotic shock without enzymatic digestion.

Islet transplantation is a potential cure for type 1 diabetes, but clinical results have been disappointing. Currently, islet isolation is by enzymatic digestion of the pancreas which has significant pitfalls: warm ischemia exposure, collagenase-induced damage to the islet mass and viability, poor reproducibility, high cost, a relatively low number of islets obtained per whole pancreas, and selection of islets for collagenase resistance rather than for glucose responsiveness. In the present study we performed a series of experiments in a porcine model to demonstrate the feasibility of a new isolation method based on selective osmotic shock (SOS) using very high glucose solutions, doubling or tripling physiological osmotic strength. The SOS method can be carried out at room temperature or in the cold eliminating warm ischemia time which damages the islets. The SOS method does not depend on the texture of the pancreas so all pancreases can be processed identically and the process can be fully automated. The SOS method isolates all the islets of the pancreas regardless of size and shape allowing a greater number of islets to be harvested. The SOS method avoids exposure to toxins in collagenase solutions, is inexpensive and selects for islets with high concentrations of Glut 2 transporters, representing the best glucose responding islets. The SOS method showed a comparable recovery of islets from young pig pancreas and the islets showed improved viability. We conclude that the selective osmotic shock (SOS) method of separating islets from the pancreatic tissue is superior to the collagenase method.

Influence of slice baling on feeding value of alfalfa hay in receiving and finishing diets for feedlot cattle.

Three studies were conducted to evaluate the feeding value of slice alfalfa hay in feedlot diets. In Exp. 1, 108 steer calves (183.1 +/- 1.2 kg initial BW; 6 pens/treatment) were used in a completely randomized design to evaluate the effect of baling method on performance and morbidity of newly received calves. The study lasted 28 d. Treatments consisted of a 65% concentrate receiving diet containing 1) ground or 2) slice alfalfa hay. Steer calves were fed daily at 0800 h. Animals also received long-stem sudangrass hay the first 7 d. Steers were weighed on d 0, 16, and 28. Feed, sudangrass hay, or feed plus sudangrass hay intakes were not affected (P > 0.25) by treatment. Conversely, ADG from d 0 to 16 was greater (P < 0.001) for slice than ground (1.27 vs. 0.81 +/- 0.067 kg/d, respectively) and from d 0 to 28 (1.23 vs. 0.91 +/- 0.042 kg/d, respectively). In addition, G:F was greater (P < 0.001) for slice than ground hay from d 0 to 16 (0.39 vs. 0.25 +/- 0.021), and from d 0 to 28 (0.31 vs. 0.24 +/- 0.013 for slice and ground, respectively). Moreover, morbidity (40.5 +/- 3.9%; P = 0.20) and retreatment rates (30.7 +/- 7.5%; P = 0.14) were similar for slice and ground. In Exp. 2, 176 crossbred steers (393.9 +/- 10.8 kg initial BW) were used in an 84-d feeding experiment (4 pens/treatment) in a randomized complete block experimental design with a 2 x 2 factorial arrangement of treatments to evaluate effects of alfalfa baling method (ground or slice) and forage level (8 or 14%) on growth performance. Experimental diets were based on steam-flaked corn. Daily BW gain was greater (P = 0.10) for steers consuming ground compared with the slice hay diet. A baling method x forage level interaction (P = 0.07) was observed for DMI. Baling method did not (P = 0.98) influence DMI with 8% roughage level. But with 14% roughage, DMI was greater (P = 0.02) for steers consuming ground hay than the slice diet. The G:F ratio was affected (P = 0.03) only by forage level (0.194 vs. 0.182 +/- 0.003 for 8 and 14% roughage, respectively). In Exp. 3, 4 ruminally cannulated mixed-breed steers were used in a 4 x 4 Latin square design to evaluate effects on digestive function. No baling method effects (P >or= 0.16) were detected for DM, OM, CP, or NDF intakes or DM, OM, and NDF total tract digestibility. Digestibility of NDF and OM were greater (P

Prebiotic ingestion does not improve gastrointestinal barrier function in burn patients.

Prebiotics increase intestinal levels of health-promoting bacteria implicated in decreasing pathogen colonization, stimulating immune functions and stabilizing gut barrier functions, parameters which are altered in burn patients. We propose that regular intake of a prebiotic, oligofructose (OF), might help to improve the altered gastrointestinal (GI) permeability observed in burn patients. A randomized, double-blind, controlled clinical trial was carried out in 41 burn patients (mean burn surface area=17.1+/-8.2%) who ingested daily 6 g of oligofructose (OF group) or sucrose as placebo (Control group) during 15 days. Gastrointestinal permeability to sucrose and lactulose/mannitol (L/M) was evaluated on days 1 (before treatment) 3, 7, 14 and 21. A permeability test was also performed in 18 healthy subjects as controls. Thirty-one patients completed the protocol (dropout rate=24.4%). Healthy subjects had a basal sucrose excretion of 21.3 mg (14.0-32.5 mg) and a basal L/M ratio of 0.017% (0.009-0.022%). Sucrose excretion increased 5-fold and L/M ratio 4.4-fold in burn patients on day 1 and these high levels of marker excretion decreased significantly throughout the study (p=0.016 and 0.000001, respectively). No differences between the OF and Control groups were observed for sucrose excretion or L/M ratio. In conclusion, the normalization of gastrointestinal permeability is not accelerated by prebiotic intake.

Use of spermicide and impact of prophylactic condom use among sex workers in Santa Fe de Bogota, Colombia.

BACKGROUND AND OBJECTIVES: Condom use is one of the most important preventive measures sex workers can take to reduce the risk of becoming infected with a sexually transmitted disease. However, a client may refuse to use a condom when requested. Some sexually transmitted disease prevention programs are recommending that sex workers use spermicide as an alternative prophylaxis when a condom is refused, yet little is known about the effect of this recommendation on prophylactic condom use. GOAL: To determine if using spermicide, either in conjunction with condoms or as a backup, influenced overall condom use among a group of sex workers at high risk of sexually transmitted diseases in Santa Fe de Bogota, Colombia. STUDY DESIGN: Participants were assigned randomly to one of three condom use groups: use of condoms only (Condoms Only), use of condoms and spermicides concurrently (Condom and Spermicide), or use of spermicide when condoms were refused (Spermicide as a Backup). A total of 199 sex workers entered the study and were asked to return for follow-up every 2 weeks for a period of 12 weeks. RESULTS: Women assigned to the Spermicide as a Backup group used a condom for an average of 78.1% of their reported acts of intercourse, compared with an average of 94.5% in the Condom Only and 92.3% in the Condom and Spermicide groups. However, women in the Spermicide as a Backup group used a condom or spermicide for an average of 96.9% of their acts of intercourse. Condoms were used for every intercourse act by less than 5% of the women in the Spermicide as a Backup groups, compared with 50.7% in the Condom Only group and 41.2% in the Condom and Spermicide group (P 0.001). When condoms were not used, client refusal was the primary reason reported. The incidence of sexually transmitted diseases and other urogenital inflammations in all groups was lower than expected. CONCLUSIONS: Among Colombian sex workers, condom use declined substantially when women were instructed to use spermicides if they were unable to persuade their partner to use a condom. However, these same women usually used the study spermicide as an alternate prophylaxis.

PIP: Some sexually transmitted disease (STD) prevention programs recommend that prostitutes use spermicide as an alternative prophylaxis when a condom is refused. 199 female sex workers in Santa Fe de Bogota, Colombia, participated in a study to assess the effect of this recommendation upon condom use. Women were randomly assigned to use condoms only, use condoms and spermicides concurrently, or use spermicide when condom use was refused. They were instructed to return for follow-up every 2 weeks for 12 weeks. Women in the spermicide-as-a-backup group used a condom for an average of 78.1% of reported acts of intercourse, compared to an average of 94.5% among the condom-only users and 92.3% among the women instructed to use both condoms and spermicide. Women in the spermicide-as-a-backup group used either a condom or spermicide for an average of 96.9% of their acts of intercourse, but less than 5% used a condom for every act of intercourse. 50.7% of women in the condom-only group and 41.2% in the condom/spermicide group used a condom for every act of intercourse. There was a lower than expected incidence of STDs and other urogenital inflammations in all groups.