Multigenerational exposure to gamma radiation affects offspring differently over generations in zebrafish.

Mutigenerational studies are now of great interest in ecotoxicology and previous studies have shown the importance of conducting multigenerational studies when assessing radiation toxicity in fish. In our study, the first objective was to study the early life stages (embryo-larval stages) and critical functions such as reproduction (which are generally studied in the context of ecological risk assessment (ERA)), in order to assess the sensitivity of zebrafish to ionizing radiation. The second objective was to assess acquisition of phenotypic effects at select life stages over generations. To our knowledge, this was the first time that irradiation of zebrafish (0.05 and 5 mGy.h-1) up to generation F2 was maintained with the following two exposure conditions: (1) recovery, only F0 genitors were irradiated and the progeny were placed in control condition, (2) irradiated condition, all generations were exposed. Multigenerational irradiation affected F1 parental reproductive capacity (reproductive success) mainly over the first reproductive cycle (104d) and larval survival rate. Unexpected yet significant effects on sex ratio were observed in F1 progeny after parental irradiation (mainly at 5 mGy.h-1). These effects were observed for both conditions -irradiated and recovery- suggesting transmitted effects from F0 genitors to offspring. All studied life stages were affected by ionizing radiation (IR), suggesting an alteration of vital physiological functions (reproduction and sexual determination). Such results highlight the hypothesis that IR affects population dynamics. In addition, the clear evidence of transmitted effects suggests worsening of effects at the population scale over generations. This approach is closer to environmental conditions to assess wild population fate, and thus highlights the importance of multigenerational studies to support ERA of ionizing radiation in fish.

Microfluidic Assisted Flash Precipitation of Photocrosslinkable Fluorescent Organic Nanoparticles for Fine Size Tuning and Enhanced Photoinduced Processes.

Highly concentrated dispersions of fluorescent organic nanoparticles (FONs), broadly used for optical tracking, bioimaging and drug delivery monitoring, are obtained using a newly designed micromixer chamber involving high impacting flows. Fine size tuning and narrow size distributions are easily obtained by varying independently the flow rates of the injected fluids and the concentration of the dye stock solution. The flash nanoprecipitation process employed herein is successfully applied to the fabrication of bicomposite FONs designed to allow energy transfer. Considerable enhancement of the emission signal of the energy acceptors is promoted and its origin is found to result from polarity rather than steric effects. Finally, we exploit the high spatial confinement encountered in FONs and their ability to encapsulate hydrophobic photosensitizers to induce photocrosslinking. An increase in the photocrosslinked FON stiffness is evidenced by measuring the elastic modulus at the nanoscale using atomic force microscopy. These results pave the way toward the straightforward fabrication of multifunctional and mechanically photoswitchable FONs, opening novel opportunities in sensing, multimodal imaging, and theranostics.

All-Solution-Processed Organic Light-Emitting Diodes Based on Photostable Photo-cross-linkable Fluorescent Small Molecules.

We demonstrate herein the fabrication of small molecule-based OLEDs where four organic layers from the hole- to the electron-transporting layers have successively been deposited by using an all-solution process. The key feature of the device relies on a novel photopolymerizable red-emitting material, made of small fluorophores substituted with two acrylate units, and displaying high-quality film-forming properties as well as high emission quantum yield as nondoped thin films. Insoluble emissive layers were obtained upon UV irradiation using low illumination doses, with no further need of postcuring. Very low photodegradation was noticed, giving rise to bright layers with a remarkable surface quality, characterized by a mean RMS roughness as low as 0.7 nm after development. Comparative experiments between solution-processed OLEDs and vacuum-processed OLEDs made of fluorophores with close architectures show external quantum efficiencies in the same range while displaying distinct behaviors in terms of current and power efficiencies. They validate the proof of concept of nondoped solution-processable emissive layers exclusively made of photopolymerized fluorophores, thereby reducing the amount of components and opening the way toward cost-effective fabrication of solution-processed OLED multilayer architectures.

Unprecedented Demonstration of Regioselective SE Ar Reaction giving Unsymmetrical Regioregular Oligothiophenes.

Aromatization of 4-cyano-3-oxotetrahydrothiophene by sulfuryl chloride gives the new building block 4-cyano-3-pyrrolidylthiophene, which forms unsymmetrical regioregular oligothiophenes with a strict alternation of the donor and acceptor groups along the conjugated system. The self-coupling reactions that form the oligomers are shown to proceed by a regioselective electrophilic aromatic substitution mechanism involving a stabilized Wheland intermediate.