Optical coherence tomography and confocal laser scanning microscopy as non-invasive tools in the diagnosis of sinonasal inverted papilloma: a pilot study.

Optical coherence tomography (OCT) and confocal laser scanning microscopy (CLSM) are light-based imaging techniques that allow for a visualization of microscopic tissue properties in vivo. Our study was to examine whether they allow for differentiation of inverted papilloma (IP) from nasal polyps (NP). Five cases of IP and NP, respectively, were investigated intraoperatively with OCT and CLSM. Biopsies were taken of the investigated area and were analyzed ex vivo with OCT and CLSM and then underwent HE-staining for standard light microscopy. On OCT images, IP showed the characteristic inverted character of the epithelium, that was thicker with a high degree of variability of thickness compared to the thin and homogenous epithelium of NP. In addition, the characteristic stromal edema of NP could be visualized. On CLSM images, the typical epithelial invaginations of IP appeared as crypts, while in NP the highly organized cylindric epithelium could be visualized. In vivo, OCT acquired images of sufficient quality to visualize these characteristics, while CLSM did not. Our study demonstrates that OCT and CLSM can distinguish IP from NP. Further technical development is required to apply the techniques clinically to guide intranasal biopsies or even to make them dispensable.

Antimicrobial susceptibility of bacterial isolates from patients presenting with ear, nose and throat (ENT) infections in the German community healthcare setting.

Empiric initial antibiotic therapy of bacterial infections is based primarily upon the susceptibility of the most common causative pathogens. The purpose of this study was to provide susceptibility data on six bacterial species known to cause ear, nose and throat (ENT) infections. A total of 1066 isolates collected during a nationwide laboratory-based surveillance study were analysed. All Streptococcus pyogenes isolates were penicillin (PEN)-susceptible, indicating that natural penicillins can still be recommended as the first-line treatment for group A streptococcal tonsillopharyngitis. Of the S. pneumoniae isolates, 92.9% were PEN-susceptible and of the Haemophilus influenzae isolates, 89.7% were amoxicillin-susceptible, retaining aminopenicillins as the first-line treatment for acute otitis media (AOM) and acute rhinosinusitis (ARS), in case antibiotic therapy is considered. In contrast, cefuroxime axetil seems less likely to be suitable for the treatment of AOM or ARS, as all Moraxella catarrhalis and >99% of the H. influenzae isolates were categorised as intermediate or resistant. The susceptibility rates of Pseudomonas aeruginosa were 97-100% for the drugs tested, except for the fluoroquinolones (87.6%). Overall, bacterial isolates from outpatients presenting with ENT infections showed low frequencies of resistance in Germany. However, given the emergence of multidrug resistance to standard antibiotics in Escherichia coli and other pathogens, inappropriate use of broad-spectrum antibiotics for the treatment of ENT infections has to be avoided.

Specific stressors in endonasal skull base surgery with and without navigation.

The goal of modern organizational psychology is to recognize, anticipate and finally avoid stress situations. The aim of this study was to measure objectively the mental and physical demands during transnasal surgery with and without the aid of a navigation system. Forty endonasal surgeries (20 with and 20 without navigation, not blinded and not randomized) done by four different experienced rhinosurgeons (>250 FESS procedures done) were included. The heart rate, the heart rate variability, the respiratory frequency and the masseter tone were monitored as biometrical parameters by the surgeons during the whole surgery for the quantification of mental demand. Stress situations could be identified during the procedures by an increase in the heart rate and a decrease in the heart rate variability. Stress level in procedures with navigation did not significantly differ from procedures without navigation. Interestingly, in 10 % of the cases a navigation system would have been helpful, although the surgeon stated before the procedure that such a system would not be necessary. Other stressors could be identified like time pressure, students or colleagues speaking with the surgeon or chatting in the OR and system failure of medical devices, i.e. navigation, sinus drill, electrocautery or shaver. Surgical stressors blurred vision due to diffuse bleeding and drill out procedures in the sphenoid sinus. Calming situations were a quiet atmosphere in the OR (i.e. closed doors) and the participation of another experienced colleague, especially a neurosurgeon. Stress situations occur when complex medical devices like the navigation do not work. For their proper function it is important that the whole OR-team is trained with it. Unqualified or unmotivated OR personnel create stress for the surgeon and disharmony in the team, which then ends in inadequate behaviour.

[Inpatient vs. outpatient costs analysis of septumplasty in Germany]. / Potenzialanalyse und Kostenbewertung bei der ambulanten Durchführung der Septumkorrektur.

The septumplasty is realized inpatient and outpatient in different countries. Caused by the pressure of reduction of expenses there is the question of the economic benefit if done outpatient in Germany. A comparison of the inpatient and outpatient gratification will be done. There is a yearly potential of cost reduction of 180 million euro, if the operation would be done as an outpatient procedure. From the hospital view actual there is no economical recommendation doing an outpatient septumplasty caused by the poor outpatient remuneration. That's why an adjustment of the outpatient remuneration should be done. Actual there is no medical or economical recommendation doing the septumplasty as an outpatient procedere.

Diode laser versus radiofrequency treatment of the inferior turbinate - a randomized clinical trial.

BACKGROUND: Laser and radiofrequency induced volume reduction of the inferior turbinates are frequently used treatment modalities. Which of both is superior, however, is not clear to date due to a lack of controlled prospective studies. Here, we compare both methods regarding improvement of nasal breathing, complications, patient comfort and wound healing. METHODOLOGY: Prospective, randomized, single-blinded clinical trial with intra-individual design. After randomization, one side of the nose was treated with a 940nm diode laser and the other side with bipolar radiofrequency therapy. Pre- and postoperative evaluation was performed using visual analogue scales, nasal endoscopy and objective measurements of nasal patency. RESULTS: Of 27 enrolled patients, 26 completed the protocol. No severe complications were observed. Intraoperative discomfort was significantly more severe on the radiofrequency side. After three months, a significant reduction of nasal obstruction was observed for laser treatment and radiofrequency therapy with no significant difference between them. Objective parameters did not improve significantly. When asked which treatment modality they would chose again 50 % of the patients decided for radiofrequency treatment, 23 % for laser treatment, and 19 % for both. CONCLUSION: DLVR and RFVR are well-tolerated treatment modalities and both significantly reduce the degree of nasal obstruction in patients with hypertrophic inferior turbinates. There was no significant difference between both treatment modalities regarding efficiency.

Effect of transtympanic low-pressure therapy in patients with unilateral Menière's disease unresponsive to betahistine: a randomised, placebo-controlled, double-blinded, clinical trial.

OBJECTIVE: To determine the effect of the Meniett low-pressure generator on the subjective symptoms and audiovestibular disease markers of patients with unilateral Menière's disease unresponsive to betahistine treatment. METHODS: Randomised, placebo-controlled, double-blinded, clinical trial at a tertiary referral centre. After ventilation tube placement, patients were randomised to the active treatment or placebo group. Monitoring comprised audiometry and air caloric testing and a vertigo diary (enabling calculation of vertigo and activity scores, and the number of vertigo days, vertigo-free days and sick days). RESULTS: Sixty-eight patients completed the study. For the active treatment versus placebo group, the following pre- and post-treatment values, and significances for treatment effect comparisons, were respectively seen: cumulative vertigo scores, 22.47 and 15.97 vs 20.42 and 19.23 (p = 0.048); vertigo days, 6.5 and 4.08 vs 5.94 and 5.52 (p = 0.102); sick days, 3.08 and 0.78 vs 2.87 and 3.45 (p = 0.041); vertigo-free days, 14.47 and 17.61 vs 15.48 and 17.58 (p = 0.362); activity score, 23.61 and 13.42 vs 24.68 and 20.23 (p = 0.078); low-tone hearing threshold, 49.15 and 53.18 dB nHL vs 41.66 and 46.10 dB nHL (p > 0.05); and slow phase velocity in response to caloric stimulation, 18.86 and 18.72 °/second vs 14.97 and 15.95 °/second, (p > 0.05). CONCLUSION: Use of the Meniett low-pressure generator improved patients' vertigo but not their hearing or vestibular function. This safe, minimally invasive treatment is recommended as second-line treatment for unilateral Menière's disease.

Double-blind, randomised, controlled study of post-operative pain in children undergoing radiofrequency tonsillotomy versus laser tonsillotomy.

INTRODUCTION: In the last decade tonsillotomy has come into vogue again, whereas the number of tonsillectomies is decreasing rapidly. Currently, most tonsils are reduced by utilise electrosurgery, radiofrequency or carbon dioxide laser. However, it is not clear whether radiofrequency tonsillotomy is as effective as laser or other surgical techniques in respect of post-operative pain and haemorrhage. MATERIAL AND METHODS: A prospective, randomised, double-blinded, controlled, clinical study was conducted in the otorhinolaryngology department of Ludwig Maximilians University, Munich, Germany. Twenty-six children with tonsillar hypertrophy were included. Exclusion criteria were: history of peritonsillar abscess, previous tonsil surgery, tonsillitis within two weeks, pain before surgery, psychiatric illness, asymmetrical tonsils, chronic analgesic usage, bleeding disorders and other surgical procedures during the same operation. Tonsillotomy was performed on one side with radiofrequency and on the other side with a carbon dioxide laser. All procedures were performed by a single surgeon, under general anaesthesia. A visual analogue scale was used to measure patients' pain on each side, administered by a 'blinded' nurse on the three post-operative mornings and evenings, within the hospital. RESULTS: There was no difference in post-operative pain scores or haemorrhage, comparing laser versus radiofrequency tonsillotomy. Patient's overall reported pain was very modest compared with post-tonsillectomy pain. No haemorrhage or other adverse effects were observed.

The influence of cholesterol on the motility of cochlear outer hair cells and the motor protein prestin.

CONCLUSIONS: We provide evidence that cholesterol reduces electromotility in a dose-dependent matter. The data show that cholesterol modulates electromotility mainly by influencing the motor protein prestin, less by affecting the passive membrane properties. OBJECTIVES: Elevated serum cholesterol is linked to inner ear disorders and may influence hearing by altering membrane properties of outer hair cells (OHCs) and by affecting the motor protein prestin. In this study we wanted to determine whether cholesterol modulates the electromotility of OHCs and if this modulation results from effects on the membrane properties or on the motor protein prestin. MATERIALS AND METHODS: The motile responses of 12 isolated OHCs were investigated at increasing concentrations of 0, 0.1, and 1 mM extracellular cholesterol using the patch clamp technique and continuous video image analysis. To study effects on prestin, experiments were performed in 12 cells with half activated protein function and concentrations of 0 and 1 mM cholesterol. RESULTS: Cholesterol at a concentration of 0.1 mM had no effect on motility. A concentration of 1 mM reduced maximal evoked shortening significantly by 29% in the depolarizing and by 9% in the hyperpolarizing direction. Investigating half activated motor proteins, 1 mM cholesterol reduced movements significantly by 18%, elongations decreased nonsignificantly by 5%.

[Otorhinolaryngological aspects of high altitude medicine]. / HNO-ärztliche Aspekte der Höhenmedizin.

The World Health Organisation estimates that about 40 million tourists every year climb to high (2,500-5,300 m) and extremely high altitudes (5,300-8,850 m). Thus altitude sickness and other health risks are increasing accordingly and so this fact requires clarification and advice for tourists in order to reduce the risks. That applies to the otolaryngologist, too. The non-traumatic health risks all result from the atmospheric conditions at high altitudes, in particular due to the lower atmospheric pressure. The partial pressure of oxygen (pO2), the temperature and the partial pressure of water vapour decrease continuously with increasing altitude and at the summit of the highest mountain on earth, Mt. Everest, the pO2 is reduced by two-thirds, from 212 to about 70 hPa. The temperature drops on average 6.5 degrees C per 1,000 m and at -20 degrees C 1 m3 of air contains at most just about 1 g of water vapour. The shortage of oxygen above 2500 m cannot be compensated for at once. Respiratory alcalosis, followed by hyperventilation, improves the alveolar loading of red blood cells (RBC) with oxygen, however, it also reduces the ventilatory drive from the central CO2-chemosensors as well from the peripheral O2-chemosensors located in the carotid bodies. Not until the alcalosis has been balanced by a renal secretion of bicarbonate, does the pO2-driven ventilatory stimulus normalize and the relative increase of RBC as a result of altitude diuresis improve and complete the acclimatisation. Up to an altitude of 4,000 m this adaptation takes several days to one week and up to 5,000 m up to 2 weeks. If acclimatisation has not taken place or has been insufficient, acute mountain sickness may develop. It is a harmless disorder, although it noticeably affects people physically and mentally and in some rare cases it might even develop into a life-threatening high-altitude edema in the brain or in the lung. Hematocrit values of up to 58 or even 60% at great altitudes are quite usual. Up to an altitude of 7,500 m the distortion product signals of the otoacustic emissions decrease not only between 1,000 and 1,500 Hz, but also between 3,000 and 4,000 Hz. The reduction of the inner ear signals, however, is reversible and disappears after descent. For the vestibular organ high altitudes do not mean a risk, either. 70% of all infections suffered by trekkers and climbers affect the upper airways. The cold, dry mountain air damages the mucociliary apparatus and thus leads a disposition towards acute recurrences in climbers suffering from chronic inflammations of the tonsils, the paranasal sinuses and the middle ear. In the oxygen-poor air these recurrences do not heal at all, or only very slowly, but also often tend to have a rather more complicated course.

Subpixel tracking for the analysis of outer hair cell movements.

CONCLUSION: Videomicroscopy with subpixel analysis is an excellent system for quantification of outer hair cell (OHC) movements. The resolution of a few nanometers is accurate enough to show induced differences of electromotility. OBJECTIVE: Electromotility of OHCs is a voltage-dependent process resulting from a membrane protein named prestin. Voltage sensitivity is conferred to prestin by intracellular anions. Reduction of these anions reduces electromotility. Videomicroscopy and subpixel tracking combine video-based analysis with a resolution of few nanometers. The aim of this study was to show the feasibility of a system for quantification of OHC movements. MATERIALS AND METHODS: Electromotility was investigated under normal and reduced intracellular chloride conditions. Cells were stimulated by the patch-clamp technique. Voltage steps were 500 ms long, ranging from -170 to +30 mV in 10 mV steps. RESULTS: As in previous studies our results show the following. The direction of OHC movement depends on the polarity of voltage steps, length changes are not equal for symmetrical voltage steps of opposite polarity, average shortening for a depolarizing step (-70 mV to +30 mV) is about 13 nm/mV. Hyperpolarization (-70 mV to -170 mV) on average evokes elongations of about 3 nm/mV. Half maximal chloride concentration reduces motility by 14%; half maximal electromotility is reached by a 94% reduction of chloride.

WITHDRAWN: [Effects of atorvastatin on presbyacusis and tinnitus : A prospective, randomized, double-blind clinical trial.] / Der Einfluss von Atorvastatin auf die Altersschwerhörigkeit und den chronischen Tinnitus : Eine prospektive, randomisierte, doppelblinde, klinische Studie.

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Bilateral and unilateral internal carotid artery dissection causing isolated hypoglossal nerve palsy: a case report and review of the literature.

Two cases of isolated hypoglossal nerve palsy due to internal carotid artery dissection are reported. In one case, a bilateral dissection was encountered. The clinical presentation and multidisciplinary management are discussed and the literature reviewed.

[The incidence of sudden hearing loss is greater than previously assumed]. / Praktische Erfahrung bestätigt. Hörsturz wesentlich häufiger als bisher angenommen.

The global incidence of sudden hearing loss is quoted to be 5-20 new cases/100,000 inhabitants/year. In the opinion of the present authors, the data on which these figures are based, are too old, methodologically questionable and non-transferable. An analysis of data from the compulsory health insurance carriers in Baden-Württemberg and the Nordrhein district suggests that an appreciably higher incidence must be assumed for Germany. Statistical processing of the numerical data of all cost carriers is necessary if we are to obtain an accurate incidence for Germany.