RESUMO
BACKGROUND: Controversial findings exist on the role of leptin in obesity and its correlation with metabolic variables, with few data emanating from Nigerian-Africans. Plasma leptin was therefore determined in obese and non-obese. Its relationship with obesity indices and metabolic variables were further envisaged. MATERIALS AND METHODS: A cross-sectional comparative study on 87 randomly-selected non-diabetes Nigerians distributed into 3 groups (24 normal, 23 pre-obese and 40 obese). Obesity indices, fasting Insulin (FI) and homeostasis model assessment-insulin resistance (HOMA-IR) were determined. Plasma leptin was determined by sandwich enzyme-linked immunosorbent assay. Mann-Whitney U-test, Independent Kruskal-Wallis test, Spearman correlation and Step-wise Multivariate Logistic Regression analysis determined outcomes. RESULTS: Median+IQR leptin concentrations were higher in obese {61.8(40.8, 91.4) ng/mL, p<0.001} than pre-obese {42.7(28.0, 51.7) ng/mL, p=0.003} and normal {28.6(20.7, 39.8) ng/mL, p=0.03} BMI categories. Leptin was higher (p<0.001) in females than males {Median+IQR, 52.0 (35.0, 80.0) ng/mL versus 34.0(24, 65.0 ng/mL)}. Leptin was positively correlated with body fat percentage (BFP), r=0.57; waist circumference (WC), r=0.46 and body mass index (BMI), r=0.50, p<0.001, respectively in all subjects, with higher correlation coefficient in males than females. Leptin showed sex-specific correlations to age, FI and HOMA-IR. In the unadjusted models, central obesity/generalized obesity, WC, BMI, BFP, combined pre-obesity/obesity, younger age and female sex were significantly (p<0.001) associated with log-transformed leptin. WC (OR: 1.2, 95% CI, 1.05-1.38, p=0.009), BFP (OR: 1.41, 95% CI, 1.07-1.84, p=0.013) and BMI (OR: 1.6, 95% CI, 1.13-2.31, p=0.008) in men and all subjects, were independently associated with hyperleptinaemia following adjustments. CONCLUSION: Plasma leptin concentrations, fasting insulin and insulin resistance are higher in obese and pre-obese than normal controls, with females showing higher leptin concentrations than males. Leptin is independently related to BMI, BFP, WC, female sex and generalized/central obesity in Nigerian-Africans especially males. It showed sex-specific relations to age, fasting insulin and HOMA-IR.
RESUMO
Most studies on leptin in diabetes mellitus (DM) compared to healthy controls were done in Caucasians, with conflicting findings. Paucity of data on this exists in Nigerian-Africans. Therefore, the study determined plasma leptin concentrations in newly diagnosed type-2 diabetes versus controls and its relation to obesity/demographic-metabolic indices. A cross-sectional comparative study on 154 subjects: 67 diabetes and 87 healthy controls at the Ahmadu Bello University Teaching Hospital, Nigeria. Leptin was determined by the sandwich enzyme-linked immunosorbent assay. Mann-Whitney U test, Spearman's Correlation and Step-wise Multiple Logistic Regression analysis of Log-transformed variables determined outcomes. Leptin trended towards lower levels in DM subjects than controls when both sexes were combined, though insignificant (P=0.12). Leptin was significantly (P<0.001) positively correlated with waist circumference (WC) and body mass index (BMI) in DM (WC, r=0.71; BMI, r=0.84) as well as controls (WC, r=0.46, BMI, r=0.51), respectively. Leptin was significantly (P<0.001) higher in females than males, with approximately 2 times Odds of female sex association with log-transformed (Ln10) hyperleptinaemia {Odds Ratio (OR): 1.9, 95% CI, 0.97-3.92, P<0.001}. Age was positively (r=0.21, P=0.05) correlated to leptin in controls, while fasting blood glucose (FBG) negatively correlated to uncontrolled DM (r=-0.26). Leptin showed no significant (P>0.05) correlation to fasting insulin (FI) and HOMA-IR. WC was an independent predictor of Ln10hyperleptinaemia in DM subjects (OR: 1.12, 95% CI, 1.03-1.23, P=0.01). BMI showed significant (P<0.001) association with Ln10hyperleptinaemia in both subjects. Conclusively, leptin trends towards lower levels but are not different in newly diagnosed DM than controls. The association of leptin with obesity is similar but stronger in diabetes than controls, with no relations to FI and HOMA-IR. WC and BMI are independent predictors of hyperleptinaemia.