Effect of foliar application of phosphorus nanoparticles on the performance and sustainable agriculture of sweet corn.

Traditional phosphorus fertilizers are necessary for plant growth but about 80-90% are lost into the surrounding environment via irrigation, therefore nano-fertilizers have been developed as slow-release fertilizers to achieve sustainable agriculture. This trial investigated the impact of the foliar application of hydroxyapatite nanoparticles (HA-NPs) as a source of nano-phosphorus (P-NPs) on two cultivars of sweet corn (yellow and white) throughout two seasons. The morphology and structure of the prepared HA-NPs were characterized via transmission electron microscopy (TEM) and X-ray diffractometry (XRD). In addition, agro-morphological criteria, chemical contents (i.e., photosynthetic pigments, phenols, indoles, minerals, etc.), and genomic template stability percentage (GTS%) were evaluated in the produced sweet corn. The application of 50 mg/l HA-NPs improved the growth characteristics, yield per hectare, leaf pigments, and chemical content of yellow sweet corn, whereas the application of 100 mg/l of HA-NPs to white sweet corn enhanced the vegetative characteristics, production, photosynthetic pigments, phenols, and indoles. The difference in results may be due to the presence of a +ve unique band with SCoT-4 and SCot-2 primers at 1250 and 470 bp in yellow and white corn treated with 50 and 100 mg/l, respectively. The minimum GTS% was recorded at a concentration of 75 mg/l for both white and yellow corn. The HA-NPs can be applied as a foliar source of P-NPs to achieve agricultural sustainability.

The impact of nanofertilizer on agro-morphological criteria, yield, and genomic stability of common bean (Phaseolus vulgaris L.).

The use of agricultural fertilizers is one of the methods to beat the desired enormous increase in universal food production. The application of nanotechnology in agriculture is regarded as one of the promising approaches to elevate crop production. Whereas mineral nutrients play a crucial role in the growth and yield of the common bean. The experiments were conducted to investigate the application effect of micronutrients as nanoparticles (MN-NPs) on the common ben plants. The trial was performed in the field in El-Menofya, Egypt, through two seasons (2019 & 2020) in a randomized complete block design with three replicates and four combinations of MN-NPs (ZnO, MnO2 and MoO3) with concentrations 0, 10, 20, 30, 40 mg/L as a foliar application. The data exhibited that the foliar application of MN-NPs significantly upgraded the vegetative growth characters, flower number/plant, photosynthetic pigments, and yield. The concentration of 40 mg/L of MN-NPs leads to improving the vegetative growth, flowering number, and yield characteristics of the common bean. While the biochemical components varied in their response to MN-NPs combinations. The recommended MN-NPs concentration to ameliorate the common bean growth and yield was 40 mg/L.

Ribosomal DNA localization on Lathyrus species chromosomes by FISH.

BACKGROUND: Fluorescence In Situ Hybridization (FISH) played an essential role to locate the ribosomal RNA genes on the chromosomes that offered a new tool to study the chromosome structure and evolution in plant. The 45S and 5S rRNA genes are independent and localized at one or more loci per the chromosome complement, their positions along chromosomes offer useful markers for chromosome discriminations. In the current study FISH has been performed to locate 45S and 5S rRNA genes on the chromosomes of nine Lathyrus species belong to five different sections, all have chromosome number 2n=14, Lathyrus gorgoni Parl, Lathyrus hirsutus L., Lathyrus amphicarpos L., Lathyrus odoratus L., Lathyrus sphaericus Retz, Lathyrus incospicuus L, Lathyrus paranensis Burkart, Lathyrus nissolia L., and Lathyrus articulates L. RESULTS: The revealed loci of 45S and 5S rDNA by FISH on metaphase chromosomes of the examined species were as follow: all of the studied species have one 45S rDNA locus and one 5S rDNA locus except L. odoratus L., L. amphicarpos L. and L. sphaericus Retz L. have two loci of 5S rDNA. Three out of the nine examined species have the loci of 45S and 5S rRNA genes on the opposite arms of the same chromosome (L. nissolia L., L. amphicarpos L., and L. incospicuus L.), while L. hirsutus L. has both loci on the same chromosome arm. The other five species showed the loci of the two types of rDNA on different chromosomes. CONCLUSION: The detected 5S and 45S rDNA loci in Lathyrus could be used as chromosomal markers to discriminate the chromosome pairs of the examined species. FISH could discriminate only one chromosome pair out of the seven pairs in three species, in L. hirsutus L., L. nissolia L. and L. incospicuus L., and two chromosome pairs in five species, in L. paranensis Burkart, L. odoratus L., L. amphicarpos L., L. gorgoni Parl. and L. articulatus L., while it could discriminate three chromosome pairs in L. sphaericus Retz. these results could contribute into the physical genome mapping of Lathyrus species and the evolution of rDNA patterns by FISH in the coming studies in future.

Genetic relationship study of some Vicia species by FISH and total seed storage protein patterns.

BACKGROUND: Genus Vicia is a member of family Fabaceae and comprises 180 to 210 species. The most important species is faba bean (Vicia faba) which is still one of the most favourable grain legumes over all the world. The genus contains some additional food crops and a number of forage plants and some other weedy strains such as Vicia angustifolia and Vicia cordata. The aim of the present investigation is to elucidate the phylogenetic relationships among four Vicia species, two species (Vicia angustifolia L. ssp. Angustifolia (2n = 12) and Vicia cordata wulfen ex Hoppe (2n = 10)) belong to section Vicia, Vicia dalmatica A. Kern (2n = 12, section Cracca), and Vicia johannis tamamsch (2n = 14, section Faba). RESULTS: Two tools have been applied to identify the genetic relationships among the examined species, double fluorescence in situ hybridization (FISH) has been used to localize the sites of 5S and 45S rDNA, and sodium dodecyl sulfate-poly acrylamide gel electrophoretic (SDS-PAGE) patterns of total seed storage protein fractions. Double FISH experiment has not shown any variation in the loci number, but the positions along the chromosomes were different; both Vicia johannis and Vicia dalmatica exhibited the same interstitial 45S rRNA gene loci, while Vicia angustifolia and Vicia cordata have shown single large stretched 45S rRNA loci almost at the terminal region of the shortest chromosome. It could be concluded from the similarity matrix among the Vicia species as computed according to Jaccard coefficient from the SDS-PAGE, that V. cordata is similar to V. angustifolia and V. dalmatica by a percentage of 73 and 69%, respectively, and the most related species to V. johannis is V. dalmatica (~ 64%). CONCLUSION: FISH and SDS-PAGE of the total seed storage proteins together reflected the similar genetic relationship among the studied species as fellows, V. angustifolia is more related to V. cordata then comes V. dalmatica and then V. johannis which is at a distal position from the other species.

The influence of MoO3-NPs on agro-morphological criteria, genomic stability of DNA, biochemical assay, and production of common dry bean (Phaseolus vulgaris L.).

Molybdenum is considered one of the most important micronutrients applied as a foliar fertilizer for common dry bean. In this study, molybdenum oxide nanoparticles (MoO3-NPs) were applied in different concentrations (0, 10, 20, 30 and 40 ppm) over two sequent seasons, 2018 and 2019, to investigate their effect on the plant morphological criteria, yield, and the genomic stability of DNA. The results showed that the application of 40 ppm MoO3-NPs as a foliar fertilizer showed preferable values of plant morphological criteria, such as the number of leaves and branches per plant, as well as the fresh and dry weight with regard to the common bean plant. In addition, the seed yield increased by 82.4% and 84.1% with 40 ppm, while the shoot residue increased by 32.2% and 32.1% with 20 ppm of MoO3-NPs during two seasons, 2018 and 2019, respectively. Furthermore, the common bean treated with 20 and 40 ppm MoO3-NPs had positive unique bands with ISSR primer 848 at 1400 bp (Rf 0.519) and with primer ISSR2M at 200 bp (Rf 0.729), respectively. In addition, SDS-PAGE reveald some proteins in seedlings which were absent in the flowering stage at 154, 102, 64, 37 and 34 KDa, which may be due to differences in plant proteins required for metabolic processes in each stage. In conclusion, the application of 40 ppm MoO3-NPs was more effective on the productivity of the common bean plants.