[Etiology of pulmonary diseases in children in Khabarovsk Region].

Microflora of upper respiratory tract in 658 children aged 1 month - 17 years hospitalized with acute pneumonia (AP), acute bronchitis (AB), recurrent obstructive bronchitis (ROB), malformation of lungs (ML) and broncho-alveolar dysplasia (BALD) were studied. Carriage rates of Streptococcus pneumoniae (up to 95%) and Haemophilus influenzae (up to 89%) in 240 children attending daycare centers and schools were determined. Etiology of infectious process was ascertained in 40% of cases. S. pneumoniae was isolated in 45% of acute cases (AP and AB) and in 25% of chronic cases (BALD). H. influenzae was isolated in 8 - 12% of acute cases and in 32% of chronic cases. In 23 - 29% of all cases of pulmonary pathology in children persistence of Enterococcus faecium was determined. There were 13 different serotypes among isolated pneumococci. In patients with pneumonia the rate of detection of S. pneumoniae and H. influenzae DNA fragments by PCR was significantly higher compared with rate of their isolation from sputum.

[Prenatal diagnosis of cytomegalovirus infection]. / Prenatal'naia diagnostika tsitomegalovirusnoi infektsii.

Prognostic value of markers of cytomegalovirus infection (CMV) in pregnant women for the neonatal status was assessed. Detection of such markers as antiCMV IgM and CMV DNA in cervical secretion by DNA dot-spot hybridization in women with a complicated course of pregnancy indicates a 5.7% risk of delivery of children with stable symptoms. Studies of antibodies to pre-early proteins (IE CMV) showed that antiCMV IgG to IE are more incident in pregnant women than antiCMV IgM; moreover, antiCMV IgG to IE but not antiCMV IgM are detected in umbilical blood. The results of detection of antiCMV IgG and IgM to IE correlated with the clinical characteristics of newborns.

[The effect of symptom-free genital herpes on the course and outcome of pregnancy]. / Vliianie bessimptomnogo genital'nogo gerpesa na techenie i iskhody beremennosti.

The incidence of primary genital herpes among women of the childbearing age in Khabarovsk is 2.6% or from 0 to 10.4 per 1000. Asymptomatic herpes simplex virus (HSV) carrier state in the genital tract was detected in 24.1 +/- 5.2% normal women. The virus-carrier state exerts untoward effects on the course of pregnancy and condition of the fetus. In women infected with HSV, the hazard of abortion is 1.8 times as frequent, premature delivery is 3.2 times as frequent, and children of such mothers have symptoms of intrauterine hypotrophy 4.5 times as frequently as normally.

[The use of an immunoglobulin in the comprehensive treatment of ophthalmic herpes]. / Primenenie immunoglobulina v kompleksnom lechenii oftal'mogerpesa.

The paper analyses results after treatment of 30 patients with herpetic keratitis by means of a specific antiherpetic immunoglobulin manufactured at the Sverdlovsk Research Institute of Virus Infections. As compared with traditional treatment, the terms of clinical recovery and the number of bed-days reduced. The preparation can be used in a complex treatment of patients with a herpetic disease.

[Tick-borne encephalitis in a northern zone of coniferous and broad-leaved forests of a focal region of the Amur River basin]. / Kleshchevoi éntsefalit na severe zony khvoino-shirokolistvennykh lesov ochagovogo regiona Priamur'ia.

The geographical variability of the population of tick-borne encephalitis (TBE) virus in the northern part of the zone of combined coniferous and broad-leaved forests was established; this variability was manifested by higher virulence and homogeneity of TBE virus strains, as shown in experiments on white mice receiving the virus extraneurally, in comparison with the southern part of the zone and by higher virus carriership of the ticks Ixodes persulcatus. With the epizootic situation remaining tense and the danger of TBE virus infection still present, TBE morbidity and mortality rates decreased in the years of the construction of the Baikal-Amur Railroad, which was due to greater attention given to measures for the prophylaxis of TBE during this period.

[Demonstration of the tick-borne encephalitis virus in ticks by means of nucleic acid hybridization]. / Indikatsiia virusa kleshchevogo éntsefalita v kleshchakh metodom gibridizatsii nukleinovykh kislot.

The method of molecular hybridization of nucleic acids (MHNA) is compared to the traditional bioprobe technique in the study of virus carriership of I. persulcatus ticks collected in the South and in the North of the area of coniferous and broad-leaved forests in the Khabarovsk Territory. Higher sensitivity of the MHNA method than that of the bioprobe was demonstrated by studying the tick pools: 4.9% versus 1.6% (1985), 18.2% versus 3.6% (1986). Virus carriership studied by MHNA in isolated ticks comprised 8.6% (1985) and 10.8% (1986) in the south of the region and 20.0% (1985)--in the north; average content of RNA of the tick-borne encephalitis virus comprised 10 pg. MHNA allows massive studies of individual ticks and better indication of the virus to be carried out; the response can be obtained in 24-48 hours.

[Effect of detergents on the hemagglutinating activity of different strains of tick-borne encephalitis virus]. / Vliianie detergentov na gemaggliutiniruiushchuiu aktivnost' razlichnykh shtammov virusa kleshchevogo éntsefalita.

A new genetic marker, sensitivity of hemagglutinins of tick-borne encephalitis virus (TBE) strains to the effect of hyamine 10-X (Hs), was used for characterization of the hemagglutinating component of freshly isolated TBE virus variants with differing neurovirulence and for strain grouping. This marked may also be used for directed search of strains highly active in HI.

[Detection of the natural heterogeneity of natural populations of tick-borne encephalitis viruses and a grouping of the strains]. / Vyiavlenie estestvennoi neodnorodnosti prirodnykh populiatsii virusa kleshchevogo éntsefalita i gruppirovka shtammov.

Eighty strains of tick-borne encephalitis virus isolated from I. persulcatus ticks collected in 1976-1981 in the same biotopes in the south and north of the zone of coniferous-broad-leaved forests, and two biotopes of the southern taiga subzone of the Khabarovsk region and Sakhalin island, as well as from the brains of fatal cases were studied. Significant differences were found between maximal and minimal values of the invasive activity in spatially separated populations of the same zone (t = 2.32, P less than 0.02). Significant interpopulation differences were also found in the value of peripheral activity (t = = 2.77, P less than 0.02) and invasivity index (t = 3.83, P less than 0.01). Natural heterogeneity of the strains was found both by previously known markers (high neurovirulence for mice, invasivity index, plaque size in SPEV cell culture, viremia in mice, stability to thiamine 10X- and a new marker proposed by us: the degree of adsorption of hemagglutinins on an ion-exchanger DEAE-Sephadex A-50. Strains of tick-borne encephalitis virus were grouped in two landscape zones, this grouping indicated the existence of significant interpopulation differences.