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1.
Chem Pharm Bull (Tokyo) ; 71(8): 661-664, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37532536

RESUMO

A colloidal silicate granulated nickel-aluminum-zirconium (CSG-NAZ) was prepared, and the chromium(VI) (Cr(VI)) ions recovery capacity was evaluated using a sodium sulfate solution in a column experiment. The amount adsorbed and breakthrough time were enhanced by decreasing the flow rate (flow rate is in the order of 3.0 > 2.0 > 0.5 mL). The breakthrough curves and model parameters were estimated using the Thomas and Yoon-Nelson models. The obtained data confirmed to fit both the Yoon-Nelson model (0.858-0.906) and the Thomas model (0.813-0.906). Additionally, Cr(VI) ions that adsorbed onto CSG-NAZ could be desorbed using a sodium sulfate solution in a column experiment. The total recovery percentage of Cr(VI) ions was 80.9% after six repetitions of adsorption/desorption. Finally, the obtained results revealed that CSG-NAZ was a candidate adsorbent for the recovery of Cr(VI) ions owing to its applicability toward a continuous system.


Assuntos
Níquel , Poluentes Químicos da Água , Zircônio , Alumínio , Hidróxidos , Adsorção , Íons , Concentração de Íons de Hidrogênio , Cinética
2.
Chem Pharm Bull (Tokyo) ; 70(9): 624-627, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36047233

RESUMO

In this study, we evaluate the desorption or recovery capacity of chromium(VI) ions using desorption solutions containing sodium hydroxide (NaOH) or sodium sulfate (Na2SO4). A complex hydroxide of nickel-aluminum-zirconium (NAZ) was prepared as the adsorbent for the removal of chromium(VI) ions. The results from repeated adsorption/desorption experiments on chromium(VI) ions using NAZ complex hydroxide were evaluated. The desorption percentage of chromium(VI) ions increased with the increase in the concentration of NaOH or Na2SO4 in the desorption solution. The determined optimal concentration of NaOH or Na2SO4 in the desorption solution was 10 mmol/L under the used experimental conditions. After three adsorption-desorption cycles, the recovery percentages of chromium(VI) ions using NaOH and Na2SO4 were 60% (total amounts adsorbed and desorbed were 102 and 61 mg/g, respectively) and 75% (total amounts adsorbed and desorbed were 96 and 72 mg/g, respectively), respectively. Additionally, we confirmed the existence of chromium on the surface of the NAZ complex hydroxide. After three adsorption/desorption cycles, the crystal structure of the NAZ complex hydroxide was maintained. These results indicated the potential of the NAZ complex hydroxide using a desorption solution containing NaOH or Na2SO4 for the recovery of chromium(VI) ions.


Assuntos
Níquel , Poluentes Químicos da Água , Adsorção , Alumínio/química , Cromo/química , Concentração de Íons de Hidrogênio , Hidróxidos/química , Íons , Cinética , Níquel/química , Hidróxido de Sódio , Poluentes Químicos da Água/química , Zircônio
3.
Molecules ; 27(8)2022 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-35458592

RESUMO

We combined a nickel-aluminum-zirconium complex hydroxide (NAZ) with colloidal silica as a binder to prepare a granulated agent for adsorbing heavy metals from aqueous media. Three samples with different particle diameters were prepared to evaluate the effects on the properties: small (NAZ-S), medium (NAZ-M), and large (NAZ-L). We confirmed the granulation of the prepared samples at a binder content of 25%. NAZ-S had the largest specific surface area and number of hydroxyl groups, followed by NAZ-M and then NAZ-L. Regarding the adsorption capacity, NAZ-S adsorbed the most chromium(VI) ions followed by NAZ-M and then NAZ-L. The binding energy of Cr(2p) at 575-577 eV was detected after adsorption, and the effects of the temperature, contact time, and pH on the adsorption of chromium(VI) ions were evaluated. We identified the following adsorption mechanism: ion exchange with sulfate ions in the interlayer region of the NAZ samples. Finally, the chromium(VI) ions adsorbed by the NAZ samples were easily desorbed using a desorption solution. The results showed that NAZ offers great potential for the removal of chromium(VI) ions from aqueous solutions.


Assuntos
Níquel , Poluentes Químicos da Água , Adsorção , Alumínio/química , Cromo/química , Concentração de Íons de Hidrogênio , Hidróxidos , Cinética , Níquel/química , Dióxido de Silício/química , Água , Poluentes Químicos da Água/química , Zircônio/química
4.
Chem Pharm Bull (Tokyo) ; 69(8): 789-795, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34334523

RESUMO

In this study, the effect of contact time, temperature, pH, and coexistences on the adsorption of phosphate ions using the complex nickel-aluminum-zirconium hydroxide (NAZ) was evaluated. Moreover, the recovery of adsorbed phosphate ions from NAZ using desorption solution with different concentrations was demonstrated. The results showed that the quantity of phosphate ions adsorbed gradually increased with time, and the adsorption equilibrium was achieved within 24 h after adsorption. This kinetic data could be well described by the pseudo-second-order model with the correlation coefficient in the value of 0.997. Additionally, the quantity of phosphate which was adsorbed increased as temperature increased, and these results corresponded well with both the Langmuir, the correlation coefficient ranged from 0.920-0.949, and Freundlich models, the correlation coefficient ranged from 0.863-0.995. These results showed that the adsorption of phosphate ion was monolayer adsorption onto the NAZ surface. The optimal pH for removal of phosphate ions from aqueous media was during 4-8. In addition, chloride, nitrate, and sulfate ions did not significantly affect to the adsorption capability of phosphate ions in the complex solution system. Finally, the phosphate ions which were adsorbed onto NAZ could be recovered using sodium sulfate solution (recovery percentage: approx. 50% using sodium sulfate solution at 1000 mmol/L). These results highlight the potential of using NAZ as the cost-effectiveness adsorbent for phosphate ions removal from aqueous media.


Assuntos
Alumínio/química , Hidróxidos/química , Níquel/química , Fosfatos/química , Zircônio/química , Adsorção , Concentração de Íons de Hidrogênio , Íons/química , Tamanho da Partícula , Propriedades de Superfície , Termodinâmica
5.
Chem Pharm Bull (Tokyo) ; 69(1): 86-91, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33390525

RESUMO

In this study, complex nickel-aluminum hydroxides were prepared at different molar ratios (NA12, NA11, NA21, NA31, and NA41), and their adsorption capability on arsenic ions (As(III)) from aqueous media was assessed. The physicochemical properties such as morphology, X-ray diffraction pattern, specific surface area, numbers of hydroxyl groups, and surface pH were investigated. In addition, the effect of contact time, temperature, and pH on the adsorption capability on As(III) was also evaluated. NA41 exerted the highest adsorption capability on As(III) comparable to other prepared adsorbents. However, the specific surface area and numbers of hydroxyl groups did not significantly affect the adsorption capability on As(III). The equilibrium adsorption of As(III) using NA41 was achieved within 24 h, and the obtained results corresponded to a pseudo-second-order model with correlation coefficient value of 0.980. Additionally, the adsorption isotherms were well described by both the Langmuir and Freundlich equations. The optimal pH condition for removal of As(III) using NA41 was found to be approximately 6-8. Finally, the adsorption mechanism of As(III) was assessed by analyzing the binding energy and elemental distribution, which indicated that the electrostatic interaction and ion exchange influenced the adsorption of As(III) under experimental conditions. These results demonstrated the potential candidate of NA41 as an effective adsorbent on As(III) removal from aqueous media.


Assuntos
Alumínio/química , Arsênio/isolamento & purificação , Hidróxidos/química , Níquel/química , Poluentes Químicos da Água/isolamento & purificação , Adsorção , Arsênio/química , Concentração de Íons de Hidrogênio , Tamanho da Partícula , Propriedades de Superfície , Poluentes Químicos da Água/química
6.
Chem Pharm Bull (Tokyo) ; 68(3): 292-297, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32115537

RESUMO

In this study, the adsorption capability of phosphate ion using a novel tri-metals complex hydroxide was evaluated for preventing the eutrophication in water environment. A nickel-aluminum-zirconium complex hydroxide (NAZ) was synthesized using each inorganic sulfate mixing ratio of 0.9 : 1.0 : 0.1 and was calcined at different temperatures. The characteristics of the NAZ were analyzed by scanning electron microscopy images, X-ray diffraction analysis, elemental distribution, and binding energy. Moreover, the amount adsorbed of phosphate ion onto uncalcined and calcined NAZ was measured. That of phosphate ions onto the uncalcined was the largest of all. These results suggested that the adsorption of phosphate ions tends to depend on the physicochemical properties (e.g., amount of hydroxyl groups, pore volumes, and pH) of the adsorbents. Moreover, the adsorption mechanism of phosphate ions was evaluated on the basis of binding energy and elemental analysis. After adsorption, the binding energy of phosphorus P (2s and 2p) peaked and the sulfur peak intensity S(2s) reduced. This result indicated that the adsorption mechanism of phosphate would be exchanged with sulfate ions.


Assuntos
Alumínio/química , Complexos de Coordenação/química , Hidróxidos/química , Níquel/química , Fosfatos/química , Zircônio/química , Adsorção , Complexos de Coordenação/síntese química , Tamanho da Partícula , Propriedades de Superfície
7.
Chem Pharm Bull (Tokyo) ; 68(1): 70-76, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31902902

RESUMO

In this study, nickel-aluminium complex hydroxides at different molar ratios (nickel-aluminium = 1 : 2, 1 : 1, 2 : 1, 3 : 1, and 4 : 1, referred to as NA12, NA11, NA21, NA31, and NA41) were prepared, and their adsorption capability for chromium(VI) ion was investigated. Firstly, physicochemical characteristics (SEM images, X-ray diffraction (XRD) patterns, specific surface area, amount of hydroxyl groups, and surface pH) of nickel-aluminum complex hydroxide were evaluated. The amount of chromium(VI) ion adsorbed onto NA11 (15.3 mg/g) was greater than that adsorbed onto the other adsorbents. This research elucidated that the amount of chromium(VI) ion adsorbed using nickel-aluminium complex hydroxide was related to the adsorbent surface properties (r = 0.818-0.875). Subsequently, the adsorbent (NA11) surface before and after adsorption of chromium(VI) ion was evaluated, and chromium energy (577 and 586 eV) detected after adsorption onto the NA11 surface. These results revealed that the NA11 surface properties were very important for the removal of chromium(VI) ion from aqueous solution. In addition, the effects of pH, contact time, and temperature on the adsorption of chromium(VI) ion were evaluated. We confirmed a high recovery percentage of chromium(VI) ion when using sodium hydroxide solution at 10-1000 mmol/L (approximately greater than 80%) in this experimental condition. Thus, NA11 is a promising adsorbent for the removal of chromium(VI) ion from aqueous solution.


Assuntos
Alumínio/química , Cromo/química , Complexos de Coordenação/química , Hidróxidos/química , Níquel/química , Adsorção , Concentração de Íons de Hidrogênio , Íons/química , Temperatura , Termodinâmica , Poluentes Químicos da Água/química
8.
Chem Pharm Bull (Tokyo) ; 67(5): 487-492, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31061375

RESUMO

A new mixed metal hydroxide adsorbent (NA11, molar ratioNi-Al = 1 : 1) was prepared and its physicochemical properties (specific surface area, amount of hydroxyl group, scanning electron microscopy images, X-ray diffraction analysis, elemental distribution, and binding energy) were studied. In addition, the amount of borate ion adsorbed using several adsorbents, including NA11, was evaluated in this study. The specific surface area of and amount of hydroxyl group in NA11 was greater than those of the other studied adsorbents. The amount of borate ion adsorbed showed similar trends to those of the specific surface area and number of hyrdroxyl groups, which indicated that the adsorption mechanism of borate ion was related to the specific surface area and the amount of hydroxyl group. After adsorption, the binding energy of boron B(1s) peaked, and the sulfur peak intensity S(2s) and S(2p) reduced. These results suggest that ion exchange between borate and sulfate ions was one of the adsorption mechanisms. Equilibrium adsorption was reached within 6 h in the case of NA11. These data were fitted into a pseudo-second-order model (r = 0.813-0.998). The solution pH affected the capacity of NA11 for adsorbing borate ion from aqueous solution. It was found that adsorbance was greatest at pH 10. Adsorption isotherm data were fitted to both the Freundlich (r = 0.986-0.994) and Langmuir (r = 0.997-0.999) isotherm equations. Collectively, it is suggested that NA11 is prospectively useful for the adsorption of borate ion from aqueous solutions.


Assuntos
Alumínio/química , Boratos/isolamento & purificação , Hidróxidos/química , Níquel/química , Águas Residuárias/análise , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos , Adsorção , Algoritmos , Complexos de Coordenação/química
9.
Water Sci Technol ; 2017(3): 913-921, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30016309

RESUMO

We prepared a variety of nickel-aluminum complex hydroxides, investigated their physicochemical properties, and evaluated their ability to adsorb phosphate ions (the molar ratios of nickel to aluminum, 1:2, 1:1, 2:1, 3:1, and 4:1, are referred to as NA12, NA11, NA21, NA31, and NA41). NA12 and NA11 have amorphous structures; their specific surface areas and the concentration of associated hydroxyl groups were greater than those of other adsorbents. The number of phosphate ions adsorbed onto NA12 and NA11 was greater than that onto other adsorbents. These results indicated that the phosphate ion adsorption is related to the specific surface area and the amount of hydroxyl groups. The adsorption isotherm data, and the effects of contact time and pH on the adsorption were investigated; our results implied that both the Freundlich equation model and the pseudo-second-order kinetic model describe the adsorption of phosphate ions by NA11. We showed that phosphate ions adsorbed onto NA11 can be desorbed by sodium hydroxide solution at different concentrations and that NA11 could be reused for at least three repeated cycles of phosphate ion adsorption and desorption. This study illustrates that NA11 has the potential for practical application as an adsorbent for phosphate ions from wastewater.


Assuntos
Alumínio/química , Níquel/química , Fosfatos/química , Água/química , Adsorção , Concentração de Íons de Hidrogênio , Cinética , Águas Residuárias , Poluentes Químicos da Água/química , Purificação da Água/métodos
10.
Chemosphere ; 196: 69-77, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29291516

RESUMO

Anaerobic ammonium oxidation (anammox) bacteria were enriched in continuous packed-bed columns with marine sediment. One column (SB-C) was packed with only marine sediment collected from a shrimp-aquaculture pond, and another column (SB-AMX) was inoculated with marine anammox bacteria (MAB) as a control. These columns were continuously fed with natural or artificial seawater including ammonium (NH4+) and nitrite (NO2-). The SB-AMX showed anammox activities from the beginning and continued for over 200 days. However, the SB-C had no nitrogen removal performance for over 170 days. After adding a bicarbonate solution (KHCO3) to the sediment-only packed column, anammox activity was observed within 13 days. The column exhibited a nitrogen removal efficiency (NRE) of 88% at a nitrogen loading rate (NLR) of 1.0 kg-N·m-3·day-1, which was comparable to the control one. A next-generation sequencing analysis revealed the predominance of MAB related to "Candidatus Scalindua spp.". In addition, the co-occurrence of sulfur-oxidizing denitrifiers was observed, which suggests their symbiotic relationship. This study suggests the applicability of MAB for in-situ bioremediation of nitrogen-contaminated marine sediments and reveals a potential microbial interaction between anammox and sulfur-oxidizing communities responsible for nitrogen and sulfur cycling in marine aquaculture systems.


Assuntos
Aquicultura , Bactérias/metabolismo , Biodegradação Ambiental , Sedimentos Geológicos/microbiologia , Nitrogênio/metabolismo , Poluentes Químicos da Água/metabolismo , Compostos de Amônio , Anaerobiose , Desnitrificação , Sedimentos Geológicos/química , Nitritos , Nitrogênio/análise , Oxirredução , Filogenia , Lagoas , Água do Mar/microbiologia , Poluentes Químicos da Água/análise
11.
Water Sci Technol ; 75(1-2): 94-105, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28067650

RESUMO

Different molar ratios of a Ni/Co binary hydroxide (NiCo82, NiCo91, and Ni100) were prepared and calcined at 270 °C (NiCo82-270, NiCo91-270, and Ni100-270). The properties of the adsorbents and the amount of adsorbed phosphate ions were evaluated. The adsorbents calcined at 270 °C had a nickel oxide structure. The amount of adsorbed phosphate ions, the amount of hydroxyl groups, and the specific surface area of the calcined adsorbents at 270 °C were greater than those of the uncalcined adsorbents. The amount of adsorbed phosphate ions was related to the amount of hydroxyl groups and the specific surface area; the correlation coefficients were 0.966 and 0.953, respectively. The adsorption isotherm data for NiCo91 and NiCo91-270 were fit to both the Freundlich and Langmuir equations. The amount of adsorbed phosphate ions increased with increasing temperature. The experimental data fit the pseudo-second-order model better than the pseudo-first-order model. A neutral pH was optimal for phosphate ion adsorption. In addition, the phosphate ions that were adsorbed onto NiCo91-270 could be recovered using sodium hydroxide, and the adsorbent was useful for the repetitive adsorption/desorption of phosphate ions. Collectively, these results suggest that NiCo91-270 is prospectively useful for the adsorption of phosphate ions from aqueous solutions.


Assuntos
Cobalto/química , Hidróxidos/química , Níquel/química , Fosfatos/química , Adsorção , Hidróxido de Alumínio/química , Concentração de Íons de Hidrogênio , Cinética , Soluções , Temperatura , Água/química
12.
Intern Med ; 54(7): 771-5, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25832940

RESUMO

Inflammatory pseudotumor (IPT) is a rare benign condition often misdiagnosed as malignancy. An 80-year-old man was referred to our clinic for an asymptomatic hepatic mass detected on plain abdominal CT. Abdominal ultrasonography identified the lesion as a poorly defined hypoechoic mass. Although a liver biopsy did not provide any evidence of malignancy, imaging modalities suggested a diagnosis of cholangiocarcinoma. The patient underwent left lobectomy, and the pathological findings were consistent with the features of xanthogranulomatous cholangitis. This case is the first report of hepatic IPT originating from xanthogranulomatous cholangitis without symptoms and illustrates the importance of obtaining a preoperative diagnosis in order to avoid a misdiagnosis of malignant tumor.


Assuntos
Ductos Biliares Intra-Hepáticos/patologia , Colangiocarcinoma/diagnóstico , Colangite/diagnóstico , Granuloma de Células Plasmáticas/diagnóstico , Hepatopatias/diagnóstico , Neoplasias dos Ductos Biliares/diagnóstico , Neoplasias dos Ductos Biliares/patologia , Biópsia , Colangite/patologia , Diagnóstico Diferencial , Erros de Diagnóstico , Diagnóstico por Imagem , Granuloma de Células Plasmáticas/patologia , Granuloma de Células Plasmáticas/cirurgia , Humanos , Hepatopatias/patologia , Hepatopatias/cirurgia , Masculino , Cuidados Pré-Operatórios , Resultado do Tratamento
13.
Nihon Shokakibyo Gakkai Zasshi ; 110(6): 1038-43, 2013 Jun.
Artigo em Japonês | MEDLINE | ID: mdl-23739737

RESUMO

A 55-year-old woman was admitted to our hospital because of diverse symptoms of portal hypertension, such as refractory ascites, diarrhea, and general malaise. Blood test revealed liver and renal dysfunction and glucose tolerance. Contrast enhancement computed tomography revealed splenic arteriovenous fistula with dilated splenic artery and vein, causing portal hypertension. The splenic arteriovenous fistula was successfully treated by percutaneous transarterial embolization, resulting in the complete recovery of the patient. Herein, we report a case of arteriovenous fistula which was successfully treated with the aid of interventional radiology.


Assuntos
Fístula Arteriovenosa/complicações , Hipertensão Portal/etiologia , Baço/irrigação sanguínea , Fístula Arteriovenosa/terapia , Ascite/etiologia , Diarreia/etiologia , Embolização Terapêutica , Feminino , Humanos , Hipertensão Portal/complicações , Pessoa de Meia-Idade
14.
J Gastroenterol Hepatol ; 28(7): 1217-22, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23432697

RESUMO

BACKGROUND AND AIM: Currently, hepatitis B virus (HBV) re-infection after liver transplantation (LT) can be almost completely suppressed by the administration of HBV reverse transcriptase inhibitors and hepatitis B immunoglobulins. However, after transplantation, there is no indicator of HBV replication because tests for the serum hepatitis B surface antigen and HBV-DNA are both negative. Therefore, the criteria for reducing and discontinuing these precautions are unclear. In this study, we examined the serum HBV core-related antigen (HBcrAg) and intrahepatic covalently closed circular DNA (cccDNA) in order to determine if these could be useful markers for HBV re-infection. METHODS: Thirty-one patients underwent LT for HBV-related liver disease at Nagasaki University Hospital from 2001 to 2010. Of these, 20 cases were followed up for more than 1 year (median follow-up period, 903 days). We measured serum HBcrAg and intrahepatic cccDNA levels in liver tissue. In addition, in nine cases, we assessed the serial changes of HBcrAg and intrahepatic cccDNA levels from preoperative periods to stable periods. RESULTS: We examined serum HBcrAg and intrahepatic cccDNA levels in 20 patients (35 samples). HBcrAg and cccDNA levels were significantly correlated with each other (r = 0.616, P < 0.001). From a clinical aspect, the fibrosis stage was significantly lower in both HBcrAg- and cccDNA-negative patients than in HBcrAg- or cccDNA-positive patients. CONCLUSIONS: HBcrAg and cccDNA were useful as HBV re-infection markers after LT. Keeping patients' HBcrAg and cccDNA negative after LT might contribute to long-term graft survival.


Assuntos
DNA Circular/análise , DNA Viral/análise , Doença Hepática Terminal/cirurgia , Antígenos do Núcleo do Vírus da Hepatite B/sangue , Vírus da Hepatite B/genética , Hepatite B/diagnóstico , Transplante de Fígado , Fígado/metabolismo , Adulto , Idoso , Biomarcadores/análise , Biomarcadores/sangue , Doença Hepática Terminal/etiologia , Feminino , Sobrevivência de Enxerto , Hepatite B/complicações , Hepatite B/prevenção & controle , Vírus da Hepatite B/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Prevenção Secundária
15.
Trop Med Health ; 40(2): 55-8, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23097620

RESUMO

Endemic areas of human T-lymphotropic virus type 1 (HTLV-1) have been reported in Japan as well as tropical Africa, Central and South America and Melanesia. The existence of two subgroups, i.e., the transcontinental and Japanese subgroups, was reported in Japan. In the present study, we provide data on the ratio of the two subgroups in each endemic area and infection foci and examine the distribution of HTLV-1 in Japan and neighboring areas. A 657 bp fragment of env region of HTLV-1 proviral genome was successfully amplified for 183 HTLV-1 positive DNA samples. The subgroup determination was done by RFLP reactions using endonucleases HpaI and HinfI. The northern part of mainland Kyushu, represented by Hirado and Kumamoto, was monopolized by the Japanese subgroup, while the transcontinental subgroup ranged from 20 to 35% in the Pacific coast areas of Shikoku (Kochi), the Ryukyu Archipelago (Kakeroma and Okinawa) and Taiwan. An interesting finding in the present study is the presence of the transcontinental subgroup in Kochi, suggesting the endemicity of the transcontinental subgroup along the Kuroshio Current.

16.
J Gastroenterol ; 47(2): 195-202, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22038554

RESUMO

BACKGROUND: Geranylgeranylacetone (GGA), an isoprenoid compound which includes retinoids, has been used orally as an anti-ulcer drug in Japan. GGA acts as a potent inducer of anti-viral gene expression by stimulating ISGF3 formation in human hepatoma cells. This drug has few side effects and reinforces the effect of IFN when administered in combination with peg-IFN and ribavirin. This study verified the anti-HCV activity of GGA in a replicon system. In addition, mechanisms of anti-HCV activity were examined in the replicon cells. METHODS: OR6 cells stably harboring the full-length genotype 1 replicon containing the Renilla luciferase gene, ORN/C-5B/KE, were used to examine the influence of the anti-HCV effect of GGA. After treatment, the cells were harvested with Renilla lysis reagent and then subjected to a luciferase assay according to the manufacturer's protocol. RESULT: The results showed that GGA had anti-HCV activity. GGA induced anti-HCV replicon activity in a time- and dose-dependent manner. GGA did not activate the tyrosine 701 and serine 727 on STAT-1, and did not induce HSP-70 in OR6 cells. The anti-HCV effect depended on the GGA induced mTOR activity, not STAT-1 activity and PKR. An additive effect was observed with a combination of IFN and GGA. CONCLUSIONS: GGA has mTOR dependent anti-HCV activity. There is a possibility that the GGA anti-HCV activity can be complimented by IFN. It will be necessary to examine the clinical effectiveness of the combination of GGA and IFN for HCV patients in the future.


Assuntos
Antiulcerosos/farmacologia , Diterpenos/farmacologia , Hepacivirus/efeitos dos fármacos , Serina-Treonina Quinases TOR/efeitos dos fármacos , Relação Dose-Resposta a Droga , Expressão Gênica/efeitos dos fármacos , Genes Reporter , Humanos , Fator Gênico 3 Estimulado por Interferon , Fosforilação , RNA Interferente Pequeno/imunologia , Replicon , Serina-Treonina Quinases TOR/fisiologia , Transfecção , Células Tumorais Cultivadas
17.
Trop Med Health ; 40(4): 117-24, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23532551

RESUMO

We conducted phylogenetic analyses and an estimation of coalescence times for East Asian strains of HTLV-1. Phylogenetic analyses showed that the following three lineages exist in Japan: "JPN", primarily comprising Japanese isolates; "EAS", comprising Japanese and two Chinese isolates, of which one originated from Chengdu and the other from Fujian; and "GLB1", comprising isolates from various locations worldwide, including a few Japanese isolates. It was estimated that the JPN and EAS lineages originated as independent lineages approximately 3,900 and 6,000 years ago, respectively. Based on archaeological findings, the "Out of Sunda" hypothesis was recently proposed to clarify the source of the Jomon (early neolithic) cultures of Japan. According to this hypothesis, it is suggested that the arrival of neolithic people in Japan began approximately 10,000 years ago, with a second wave of immigrants arriving between 6,000 and 4,000 years ago, peaking at around 4,000 years ago. Estimated coalescence times of the EAS and JPN lineages place the origins of these lineages within this 6,000-4,000 year period, suggesting that HTLV-1 was introduced to Japan by neolithic immigrants, not Paleo-Mongoloids. Moreover, our data suggest that the other minor lineage, GLB1, may have been introduced to Japan by Africans accompanying European traders several centuries ago, during or after "The Age of Discovery." Thus, the results of this study greatly increase our understanding of the origins and current distribution of HTLV-1 lineages in Japan and provide further insights into the ethno-epidemiology of HTLV-1.

18.
J Med Virol ; 81(8): 1450-6, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19551824

RESUMO

Peripheral blood samples were collected from 23 human T-lymphotropic virus type-1 (HTLV-1) carriers residing in Kakeroma Island, Japan (Kagoshima Prefecture, Oshima County, Setouchi Town), one of the most highly endemic areas in Japan. The samples were subjected to amplification by PCR and sequencing of the Long Terminal Repeat in order to reconstruct a phylogenetic tree of HTLV-1 isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of env region was also conducted for subgrouping of HTLV-1. Although one sample could not be amplified by PCR, and three more could not be sequenced due to the existence of conspicuous nonspecific bands or repeated sequences, the phylogenetic analysis revealed that the remaining 19 isolates obtained from Kakeroma Island belonged to either the Transcontinental or the Japanese subgroups of the Cosmopolitan subtype, one of the three major subtypes. The RFLP data corresponded closely with the typing data throughout the sequencing. The proportion of the Transcontinental subgroup among the isolates was 26.3% (5 of 19) by sequence analysis and 27.3% (6 of 22) by RFLP. Unlike in Taiwan, China and Okinawa, the Japanese subgroup was dominant in Kakeroma Island. The analysis would also suggest that the Japanese subgroup seems not to have derived from the Transcontinental subgroup, but rather that the Transcontinental subgroup came to Japan first and was followed later by the Japanese one.


Assuntos
Portador Sadio/virologia , Infecções por HTLV-I/virologia , Vírus Linfotrópico T Tipo 1 Humano/classificação , Vírus Linfotrópico T Tipo 1 Humano/genética , Sangue/virologia , Análise por Conglomerados , Genótipo , Vírus Linfotrópico T Tipo 1 Humano/isolamento & purificação , Humanos , Japão , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Análise de Sequência de DNA , Homologia de Sequência
19.
Nutr Res ; 29(2): 89-93, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19285598

RESUMO

Nutritional support may play an important role in management of liver cirrhosis (LC) associated with unresectable hepatocellular carcinoma (HCC). Total protein and albumin deteriorate in patients with LC undergoing trans-arterial chemoembolization (TACE). Therefore, in this study, we examined the hypothesis that short-term administration of branched-chain amino acids (BCAA) will prevent a fall in total protein and albumin in the perioperative period. The subjects were 56 patients who underwent TACE for HCC between 2004 and 2005 at Nagasaki University Hospital. The patients were randomly placed in the BCAA group (n = 28) or a control group (n = 28). The patients in the BCAA group consumed a snack containing 50 g of BCAA once a day at 10:00 pm starting 1 day before TACE and continuing until 2 weeks after TACE. A comparison of baseline and end point data showed greater decreases in the concentrations of total protein, albumin, cholinesterase, and total cholesterol and in the red blood cell count in the control group compared to the BCAA group. Ammonia levels decreased in the BCAA group and increased in the control group. Our findings indicate that a BCAA supplement taken orally as a late evening snack prevents suppression of liver function by TACE in patients with LC complicated with HCC during the 2-week period after TACE.


Assuntos
Aminoácidos de Cadeia Ramificada/uso terapêutico , Carcinoma Hepatocelular/terapia , Quimioembolização Terapêutica/efeitos adversos , Cirrose Hepática/dietoterapia , Neoplasias Hepáticas/terapia , Albumina Sérica , Idoso , Aminoácidos de Cadeia Ramificada/administração & dosagem , Amônia/sangue , Proteínas Sanguíneas/metabolismo , Carcinoma Hepatocelular/complicações , Colesterol/sangue , Colinesterases/sangue , Suplementos Nutricionais , Contagem de Eritrócitos , Feminino , Alimentos Fortificados , Humanos , Cirrose Hepática/sangue , Cirrose Hepática/etiologia , Neoplasias Hepáticas/complicações , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
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