Valorization of non-edible fruit seeds into valuable products: A sustainable approach towards circular bioeconomy.

Global imperatives have recently shown a paradigm shift in the prevailing resource utilization model from a linear approach to a circular bioeconomy. The primary goal of the circular bioeconomy model is to minimize waste by effective re-usage of organic waste and efficient nutrient recycling. In essence, circular bioeconomy integrates the fundamental concept of circular economy, which strives to offer sustainable goods and services by leveraging biological resources and processes. Notably, the circular bioeconomy differs from conventional waste recycling by prioritizing the safeguarding and restoration of production ecosystems, focusing on harnessing renewable biological resources and their associated waste streams to produce value-added products like food, animal feed, and bioenergy. Amidst these sustainability efforts, fruit seeds are getting considerable attention, which were previously overlooked and commonly discarded but were known to comprise diverse chemicals with significant industrial applications, not limited to cosmetics and pharmaceutical industries. While, polyphenols in these seeds offer extensive health benefits, the inadequate conversion of fruit waste into valuable products poses substantial environmental challenges and resource wastage. This review aims to comprehend the known information about the application of non-edible fruit seeds for synthesising metallic nanoparticles, carbon dots, biochar, biosorbent, and biodiesel. Further, this review sheds light on the potential use of these seeds as functional foods and feed ingredients; it also comprehends the safety aspects associated with their utilization. Overall, this review aims to provide a roadmap for harnessing the potential of non-edible fruit seeds by adhering to the principles of a sustainable circular bioeconomy.

Anti-oxidant potential of plants and probiotic spp. in alleviating oxidative stress induced by H2O2.

Cells produce reactive oxygen species (ROS) as a metabolic by-product. ROS molecules trigger oxidative stress as a feedback response that significantly initiates biological processes such as autophagy, apoptosis, and necrosis. Furthermore, extensive research has revealed that hydrogen peroxide (H2O2) is an important ROS entity and plays a crucial role in several physiological processes, including cell differentiation, cell signalling, and apoptosis. However, excessive production of H2O2 has been shown to disrupt biomolecules and cell organelles, leading to an inflammatory response and contributing to the development of health complications such as collagen deposition, aging, liver fibrosis, sepsis, ulcerative colitis, etc. Extracts of different plant species, phytochemicals, and Lactobacillus sp (probiotic) have been reported for their anti-oxidant potential. In this view, the researchers have gained significant interest in exploring the potential plants spp., their phytochemicals, and the potential of Lactobacillus sp. strains that exhibit anti-oxidant properties and health benefits. Thus, the current review focuses on comprehending the information related to the formation of H2O2, the factors influencing it, and their pathophysiology imposed on human health. Moreover, this review also discussed the anti-oxidant potential and role of different extract of plants, Lactobacillus sp. and their fermented products in curbing H2O2­induced oxidative stress in both in-vitro and in-vivo models via boosting the anti-oxidative activity, inhibiting of important enzyme release and downregulation of cytochrome c, cleaved caspases-3, - 8, and - 9 expression. In particular, this knowledge will assist R&D sections in biopharmaceutical and food industries in developing herbal medicine and probiotics-based or derived food products that can effectively alleviate oxidative stress issues induced by H2O2 generation.

Effect of selected physical and chemical modifications on physicochemical, pasting, and morphological properties of underutilized starch from rice bean (Vigna umbellata).

Starch was extracted from the rice bean which is largely underutilized and modified by physical (i.e. heat moisture treatment and retrogradation) and chemical (i.e. esterification and acid alcohol modification) methods. Both, physical and chemical modifications significantly (p < 0.05) affected the physicochemical, pasting, particle size and morphological properties of rice bean starch. Both amylose content and swelling power reduced after physical and chemical modifications. Among modified starches, retrograded starch showed higher solubility (8.56%) at 90 °C. Retrogradation also resulted in higher values of water (251%) and oil absorption (106%) capacities in comparison to other modified starches. Physical modifications greatly influenced the pasting properties in comparison to chemical modifications. The particle size distribution followed the order: native starch (659.8 nm) > heat moisture treated (434.3 nm) > retrograded (355.4 nm) > esterified (218 nm) > acid alcohol treated starch (234.5 nm). The study revealed that the particle size of rice bean starch was reduced by both physical and chemical modifications. FE-Scanning electron microscopy was used to study the morphological characteristics of starches and it was observed that retrogradation had a pronounced effect on the starch granules morphology.

Synthesis, characterization, and utilization of potato starch nanoparticles as a filler in nanocomposite films.

The nanoparticles for the preparation of nanocomposite starch films were synthesized from potato starch using the acid hydrolysis method. The films were prepared by incorporating starch nanoparticles into the film formulation at 0.5, 1, 2, 5, and 10% level of total starch. The control starch film was prepared without the incorporation of starch nanoparticles (SNPs) in film formulation. The starch and SNPs were analyzed for physicochemical and morphological properties. The absorption capacity of SNPs for water and oil was significantly (p < 0.05) lower as compared to native starch. Whereas, the swelling power and solubility of SNPs were significantly (p < 0.05) higher than the swelling power and solubility of starch, respectively. The starch granules were oval and spherical with regular surfaces whereas the SNPs had irregular cracked exteriors spaces. The water vapor transmission rate (WVTR) from nanocomposite starch films was significantly (p < 0.05) lower than the control starch film. The burst strength of films was increased significantly (p < 0.05) with an increased level of SNPs incorporation in film formulation. The incorporation of SNPs increased film thickness and biodegradability. Thus, the present study revealed that the incorporation of SNPs in film formulation resulted in improved film properties.

Impact on various properties of native starch after synthesis of starch nanoparticles: A review.

In recent years, interdisciplinary research is more focused on particle size, which helps in exploring the relation between micro and macroscopic properties of various materials. Starch nanoparticles are generally synthesized by using acid/enzymatic hydrolysis, gamma irradiation, simple nanoprecipitation, ultra-sonication, and homogenization treatments. The properties like amylose content, pasting, rheological, morphological, size distribution, etc. are affected after the formation of nanoparticles from starch. This study emphasizes how various properties are changed in starch nanoparticles. Starch nanoparticles are mainly used in the formulation of nano-emulsion, nano starch-based composite film, and drug delivery. The impact on various native starch properties after the preparation of starch nanoparticles are less reported. So, all the aspects related to various starch properties and their nanoparticles are extensively reviewed in this study so that the listed findings can be utilized in future processes to increase the various foods and non-food utilization of starch nanoparticles.

Synthesis and characterization of nano starch-based composite films from kidney bean (Phaseolus vulgaris).

This study was aimed to synthesize and evaluate the nano starch-based composite films by the addition of nano starch in film formulation at 0.5, 1, 2, 5 and 10% level of total starch. The acid hydrolysis technique was used to reduce the size of starch granules of kidney bean starch. The physicochemical properties of both native and nano starch were determined. Nano starch showed a higher value for swelling power, solubility, water and oil absorption capacity when compared with native starch. The particle size of kidney bean nano starch was 257.7 nm at 100% intensity. The size of starch granule affects various properties of films. The thickness, solubility and burst strength of the composite films were increased significantly (p ≤ 0.05) with an increase in the concentration of nano starch in film formulation. While the moisture content and water vapour transmission rate (WVTR) were decreased significantly (p ≤ 0.05) with an increase in the concentration of nano starch in film formulation. The results suggested that kidney bean starch could be used for the development of packaging films. The utilization of nano starch in film formulations had an additional advantage in improving the film properties.

Development and characterization of nano starch-based composite films from mung bean (Vigna radiata).

The excessive use of disposable plastic material in our society demands packaging material which can undergo quick degradation without harming the environment. Agricultural products can serve as one of the essential sources for the production of biodegradable packaging material. In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied. The average particle size distribution of nano starch was 141.772 nm. Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface. Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method. The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed. With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.

Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).

In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated. Nano starch was prepared by adopting the acid hydrolysis method having a yield of 27.5%. Particle size distribution of native and nano starch was 5559 nm and 396 nm. The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface. While the water chestnut nano starch appeared as an agglomerated form with irregular and rough surface. Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method. The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated. The results showed that native starch film had thickness (0.041 ±â€¯0.07 mm) moisture content (4.17 ±â€¯0.32%), water vapour transmission rate (4.678 × 10-3 ±â€¯0.42 g-2 s-1), water solubility (35.71 ±â€¯0.17%) and burst strength (976.4 ±â€¯12.47 g), respectively. The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.

Effect of Egg Albumen, Vegetable Oil, Corn Bran, and Cooking Methods on Quality Characteristics of Chicken Nuggets Using Response Surface Methodology.

Response surface methodology was used to study the effect of egg albumen (5-15 g), vegetable oil (5-15 g), and corn bran (5-15 g) on sensory and textural (firmness and toughness) quality of chicken nuggets cooked by the oven, steam, and microwave methods. The egg albumen and vegetable oil had a positive linear effect but corn bran had a negative linear effect at p<0.01 on sensory overall acceptability scores of nuggets. Firmness and toughness scores were increased significantly (p<0.01) with the increase in corn bran level in the formulation. The optimum level of egg albumen, vegetable oil, and corn bran were obtained and validated. Cooking methods also affected the sensory and textural quality of nuggets. Steam cooked nuggets had higher values of sensory scores than oven and microwave cooked nuggets. Oven cooked nuggets showed higher values of firmness and toughness than steam and microwave cooked nuggets. Results of this study suggest that emulsion based meat products can be enriched with dietary fiber source like corn bran without compromising the sensory and textural quality of the products.

Production of tannase under solid-state fermentation and its application in detannification of guava juice.

Guava juice is known to be rich in antioxidant activity due a high level of vitamins A and C. However, tannins present in the guava juice form tannin-protein complexes that affect the utilization of vitamins and minerals and inhibit digestive enzymes. Beside this, bitterness and cloudiness are the other major problems of juice industries. The present study aimed to utilize a low-cost substrate (tea residue) for the production of tannase and its application in detannification of guava juice. Solid-state fermentation (SSF) was evaluated to produce tannase from Aspergillus niger. Maximum tannase (1.86 U/g dry substrate) production was observed at 30°C after 96 hr of the incubation period. The optimum pH of the moistening agent was found to be 5.0. Partially purified enzyme using ammonium sulfate precipitation was subjected to guava juice treatment at a level of 0.5, 1.0, and 2.0% for 30 and 60 min. Decrease in tannic acid content of guava juice was found to be 17.60, 29.04, and 44.38% after 30 min and 40.59, 53.69, and 59.23% after 60 min, respectively.