RESUMO
Apolipoprotein L-I (apoL1) is a human-specific serum protein that kills Trypanosoma brucei through ionic pore formation in endosomal membranes of the parasite. The T. brucei subspecies rhodesiense and gambiense resist this lytic activity and can infect humans, causing sleeping sickness. In the case of T. b. rhodesiense, resistance to lysis involves interaction of the Serum Resistance-Associated (SRA) protein with the C-terminal helix of apoL1. We undertook a mutational and deletional analysis of the C-terminal helix of apoL1 to investigate the linkage between interaction with SRA and lytic potential for different T. brucei subspecies. We confirm that the C-terminal helix is the SRA-interacting domain. Although in E. coli this domain was dispensable for ionic pore-forming activity, its interaction with SRA resulted in inhibition of this activity. Different mutations affecting the C-terminal helix reduced the interaction of apoL1 with SRA. However, mutants in the L370-L392 leucine zipper also lost in vitro trypanolytic activity. Truncating and/or mutating the C-terminal sequence of human apoL1 like that of apoL1-like sequences of Papio anubis resulted in both loss of interaction with SRA and acquired ability to efficiently kill human serum-resistant T. b. rhodesiense parasites, in vitro as well as in transgenic mice. These findings demonstrate that SRA interaction with the C-terminal helix of apoL1 inhibits its pore-forming activity and determines resistance of T. b. rhodesiense to human serum. In addition, they provide a possible explanation for the ability of Papio serum to kill T. b. rhodesiense, and offer a perspective to generate transgenic cattle resistant to both T. b. brucei and T. b. rhodesiense.
Assuntos
Apolipoproteínas/fisiologia , Sobrevivência Celular/efeitos dos fármacos , Lipoproteínas HDL/fisiologia , Glicoproteínas de Membrana/metabolismo , Proteínas de Protozoários/metabolismo , Trypanosoma brucei brucei/fisiologia , Trypanosoma brucei rhodesiense/fisiologia , Sequência de Aminoácidos , Animais , Apolipoproteína L1 , Apolipoproteínas/genética , Apolipoproteínas/metabolismo , Apolipoproteínas/farmacologia , Análise Mutacional de DNA , Humanos , Zíper de Leucina/genética , Lipoproteínas HDL/genética , Lipoproteínas HDL/metabolismo , Lipoproteínas HDL/farmacologia , Camundongos , Camundongos Transgênicos , Dados de Sequência Molecular , Mutação , Papio anubis , Proteínas Citotóxicas Formadoras de Poros/genética , Proteínas Citotóxicas Formadoras de Poros/metabolismo , Proteínas Citotóxicas Formadoras de Poros/farmacologia , Ligação Proteica , Alinhamento de Sequência , Termodinâmica , Tripanossomicidas/metabolismo , Tripanossomicidas/farmacologia , Trypanosoma brucei brucei/metabolismo , Trypanosoma brucei rhodesiense/metabolismoRESUMO
African trypanosomes (the prototype of which is Trypanosoma brucei brucei) are protozoan parasites that infect a wide range of mammals. Human blood, unlike the blood of other mammals, has efficient trypanolytic activity, and this needs to be counteracted by these parasites. Resistance to this activity has arisen in two subspecies of Trypanosoma brucei - Trypanosoma brucei rhodesiense and Trypanosoma brucei gambiense - allowing these parasites to infect humans, and this results in sleeping sickness in East Africa and West Africa, respectively. Study of the mechanism by which T. b. rhodesiense escapes lysis by human serum led to the identification of an ionic-pore-forming apolipoprotein - known as apolipoprotein L1 - that is associated with high-density-lipoprotein particles in human blood. In this Opinion article, we argue that apolipoprotein L1 is the factor that is responsible for the trypanolytic activity of human serum.
Assuntos
Apolipoproteínas/fisiologia , Soros Imunes/imunologia , Lipoproteínas HDL/fisiologia , Trypanosoma brucei gambiense/fisiologia , Trypanosoma brucei rhodesiense/fisiologia , Tripanossomíase Africana/imunologia , Animais , Antígenos de Neoplasias/fisiologia , Apolipoproteína L1 , Proteínas Sanguíneas/fisiologia , Haptoglobinas/fisiologia , Humanos , Trypanosoma brucei gambiense/imunologia , Trypanosoma brucei rhodesiense/imunologia , Tripanossomíase Africana/parasitologiaRESUMO
Apolipoprotein L-I is the trypanolytic factor of human serum. Here we show that this protein contains a membrane pore-forming domain functionally similar to that of bacterial colicins, flanked by a membrane-addressing domain. In lipid bilayer membranes, apolipoprotein L-I formed anion channels. In Trypanosoma brucei, apolipoprotein L-I was targeted to the lysosomal membrane and triggered depolarization of this membrane, continuous influx of chloride, and subsequent osmotic swelling of the lysosome until the trypanosome lysed.
Assuntos
Apolipoproteínas/química , Apolipoproteínas/metabolismo , Membranas Intracelulares/metabolismo , Lipoproteínas HDL/química , Lipoproteínas HDL/metabolismo , Lisossomos/metabolismo , Trypanosoma brucei brucei/metabolismo , Ácido 4,4'-Di-Isotiocianoestilbeno-2,2'-Dissulfônico/farmacologia , Sequência de Aminoácidos , Animais , Ânions/metabolismo , Apolipoproteína L1 , Apolipoproteínas/genética , Apolipoproteínas/farmacologia , Células Imobilizadas , Cloretos/metabolismo , Colicinas/química , Colicinas/farmacologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Humanos , Membranas Intracelulares/efeitos dos fármacos , Membranas Intracelulares/ultraestrutura , Canais Iônicos/metabolismo , Bicamadas Lipídicas/química , Lipoproteínas HDL/genética , Lipoproteínas HDL/farmacologia , Lisossomos/efeitos dos fármacos , Lisossomos/ultraestrutura , Modelos Moleculares , Dados de Sequência Molecular , Mutação , Permeabilidade , Conformação Proteica , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Proteínas Recombinantes/metabolismo , Trypanosoma brucei brucei/efeitos dos fármacos , Trypanosoma brucei brucei/ultraestruturaRESUMO
The heterodimeric transferrin receptors of Trypanosoma brucei (Tf-Rs) are encoded by two genes termed ESAG7 and ESAG6. These genes belong to polycistronic transcription units contained in the multiple expression sites for the variant surface glycoprotein (VSG ESs), only one of which is active at a time. Each VSG ES carries a different copy of these genes, leading to alternative expression of Tf-Rs with quite distinct binding affinities for transferrins from various mammals. T. brucei clones exhibit marked growth differences depending on the species-specificity of the serum. Since transferrin is a vital growth factor for the parasite, we investigated whether it could be responsible for these observations. We analyzed the cases of Tf-Rs from two ESs preferentially selected for expression in man and mouse, respectively. We show that serum-dependent growth variations of trypanosomes expressing these receptors are independent of transferrin, and that both high- and low-affinity Tf-Rs allow efficient trypanosome growth in various sera, either in vivo or in vitro.
Assuntos
Receptores da Transferrina/metabolismo , Transferrina/fisiologia , Trypanosoma brucei brucei/crescimento & desenvolvimento , Animais , Bovinos , Meios de Cultura , Humanos , Soros Imunes/imunologia , Camundongos , Coelhos , Receptores da Transferrina/genética , Especificidade da Espécie , Transferrina/genética , Transferrina/imunologia , Trypanosoma brucei brucei/imunologiaRESUMO
Human sleeping sickness in east Africa is caused by the parasite Trypanosoma brucei rhodesiense. The basis of this pathology is the resistance of these parasites to lysis by normal human serum (NHS). Resistance to NHS is conferred by a gene that encodes a truncated form of the variant surface glycoprotein termed serum resistance associated protein (SRA). We show that SRA is a lysosomal protein, and that the amino-terminal alpha-helix of SRA is responsible for resistance to NHS. This domain interacts strongly with a carboxy-terminal alpha-helix of the human-specific serum protein apolipoprotein L-I (apoL-I). Depleting NHS of apoL-I, by incubation with SRA or anti-apoL-I, led to the complete loss of trypanolytic activity. Addition of native or recombinant apoL-I either to apoL-I-depleted NHS or to fetal calf serum induced lysis of NHS-sensitive, but not NHS-resistant, trypanosomes. Confocal microscopy demonstrated that apoL-I is taken up through the endocytic pathway into the lysosome. We propose that apoL-I is the trypanosome lytic factor of NHS, and that SRA confers resistance to lysis by interaction with apoL-I in the lysosome.