Etiological factors of bloodstream infections in oncological patients, who was hospitalized at the National Institute of Maria Sklodowska-Curie - National Research Institute in Warsaw in 2020-2022.

Aim of the study: The purpose of the study was the microbiological analysis of bloodstream infections in patients hospitalized at the National Institute of Oncology, Maria Sklodowska-Curie - National Research Institute in the period from 01/01/2020 to 31/10/2022. Material and methods: In the period from 01/01/2020 to 31/10/2022, 18,420 blood cultures obtained from patients hospitalized at the NIO-PIB were analysed in the Department of Clinical Microbiology (total for the presence of bacteria and fungi). Culture for the presence of bacteria was carried out in the BactAlert automatic system by bioMerieux, and for fungi in the Bactec FX automatic system by Becton Dickinson. Results: 1,184 strains of bacteria and 32 strains of fungi considered to be the etiological factor of the infection were cultured from clinical samples. Gram-positive bacteria accounted for 61.57%, while Gram-negative bacteria accounted for 32.26% of all isolated bacterial strains. The most frequently cultured strains were Escherichia coli - 13.77% (including 22.1% of ESBL strains), Klebsiella penumoniae - 4.6% (44.4% of ESBL strains, 1.85% of NDM strains), Enterobacter cloacae - 2 .7% (including 40.6% of multi-resistant strains: ESBL (15.6%) or with AmpC derepression (25%), among the non-fermenting bacilli, Pseudomonas aeruginosa was the most frequently cultured - 4.18% (including 3.8% MBL) and Acinetobacter baumannii - 0.8% (including CRAB strains 50%, MBL 10%). Anaerobic microorganisms were responsible for 3.46% of blood infection cases. Yeast- like fungi were a factor in 2.7% of all fungemia cases. From blood samples taken Staphylococci were more frequently isolated directly from a vein or through a central venous catheter than aerobic Gram-negative bacilli (44.7% and 25.3% and 55.6% and 12.5%, respectively). The opposite situation occurred in the case of samples taken simultaneously directly from vein and through a central venous catheter, in which a higher share of aerobic Gram-negative bacilli (46.6%) than staphylococci (32.8%) in causing blood infections was observed. Conclusions: Gram-positive bacteria are the major contributors to bloodstream infections in cancer patients. There is a growing tendency to develop BSI caused by multi-resistant strains.

Deepfakes and Democracy (Theory): How Synthetic Audio-Visual Media for Disinformation and Hate Speech Threaten Core Democratic Functions.

Observers fear that deepfakes will shake the very foundations of democracy. Notwithstanding, in-depth scholarly analyses of deepfakes' political impact are rare, and do not consider theories of democracy. This contribution helps close this research gap, drawing on Warren's problem-oriented democracy theory, as well as theories of deliberative democracy and contributions on the role of trust in democracies. I identify three core functions of democratic systems and their normative foundations, namely empowered inclusion, collective agenda and will formation (supported by deliberation), and collective decision-making. Based on a literature and media analysis, I systematize different types of deepfakes serving either disinformation or hate speech and outline how they weaken core democratic functions and norms: Deepfakes impede citizens' empowered inclusion in debates and decisions that affect them, e.g. by hampering efforts to hold political representatives accountable or further marginalizing certain societal groups such as women or ethnic minorities. Deepfakes also undermine collective agenda and will formation by threatening the epistemic quality of deliberation as well as citizens' mutual empathy and respect. This culminates in a decreased legitimacy of collective decisions taken, which is additionally threatened by pervasive (but mostly speculative) fears of deepfake election manipulation. My analysis has implications for (future) governance efforts addressing deepfakes. Such efforts are increasing, e.g. on the part of social media platforms, but also (supra-)national regulatory bodies.

[The colonization of the digestive tract multidrug-resistant strains hospitalized patients in Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology].

INTRODUCTION: The aim of the study was a retrospective analysis of intestinal flora for the presence of multidrug-resistant strains, isolated from patients hospitalized in clinics Oncology Center from 01.01.2010 to 30.09.2015 r. METHODS: The multi-resistant strains were isolated from stool and rectal swabs. In order to increase the potential of multiple-resistant strains, the material was plated on the appropriate substrate. Determination of resistance mechanisms performed by general recommendations. RESULTS: Results of this study showed among isolated multiple-resistance strains a high proportion of Enterobacteriaceae strains producing ß-lactamase mainly ESBL. Klebsiella pneumoniae consist of 31.9% of isolated strains, E. coli 28.74% and Enterococcus faecium VER -21.15%. CONCLUSIONS: It is important to determine the microbiological status of hospitalized patients because colonized gastrointestinal tract multi-resistant strains may be one of the sources of serious infections.

[Etiology of infection associated with neoplastic changes of organs in the head and neck in patients hospitalized in the Oncology Centre in Warsaw].

INTRODUCTIONS: According to clinical observations primary neoplastic lesions in the head and neck are often complicated by infection. The incidence of postoperative complications of cancer lesions of the head and neck, oral cavity, upper airways or larynx is from 19% up to 47% cases but in extensive and long-term operations can reach 80%. METHODS: We retrospectively evaluated results of microbiological investigations of 312 clinical specimens collected from patients from Clinical Head and Neck Cancers of On- cology Center in Warsaw in the years 2008 -2012. All samples of clinical materials were plated on the suitable culture media and incubated according to the recommendations. RESULTS: The study has shown that from all collected clinical specimens 491 strains have been isolated. Nearly half of the cultured bacteria were Gram-positive cocci (48.7%), mainly methicillin-sensitive Staphylococcus aureus. Most Gram-negative bacteria isolated from neoplastic lesions in the head and neck belong to Enterobacteriaceae (21%), 23,4% of cultured bacteria grown anaerobically and they were mainly Gram-negative rods. CONCLUSIONS: The presence of diverse bacterial flora colonizing the mucous membranes of the mouth and throat may be the reason of difficulties in interpretation of microbiological investigation. It must be remembered that knowledge of colonizing and pathogenic flora of the area of head and neck enables appropriate preoperative prevention and empiric therapy.