RESUMO
Tannic acid (TA) exhibits low bioavailability in the gastrointestinal tract, limiting its benefits due to small amounts reaching the CNS. Thus, the objective of this study was to develop zein capsules and fibers by electrospraying/electrospinning for encapsulation of TA. Polymeric solutions were evaluated by electrical conductivity, density, and viscosity. In zein capsules, up to 2 % TA was added, and in fibers, up to 1 % TA was added. Zein capsule and fiber with TA were evaluated by morphology, size distribution, encapsulation efficiency, thermal and thermogravimetric properties, and functional groups. Zein capsule with 1.5 % TA was evaluated in astrocyte culture for cytotoxicity and antioxidant activity. TA zein capsules and fibers exhibited high encapsulation efficiency and homogeneous morphology. TA encapsulated in zein presented higher thermal stability than free TA. TA zein capsule did not present toxicity and elicited antioxidant action in lipopolysaccharide-induced astrocyte culture. Capsules and fibers were successfully produced by electrospraying/electrospinning techniques.
Assuntos
Antioxidantes , Astrócitos , Lipopolissacarídeos , Polifenóis , Taninos , Zeína , Taninos/química , Taninos/farmacologia , Astrócitos/efeitos dos fármacos , Astrócitos/metabolismo , Zeína/química , Antioxidantes/farmacologia , Antioxidantes/química , Lipopolissacarídeos/farmacologia , Animais , Escherichia coli/efeitos dos fármacos , Ratos , Células Cultivadas , CápsulasRESUMO
The aim of this study was to investigate the antiglioma effect of Cecropia pachystachya Trécul (CEC) leaves extract against C6 and U87 glioblastoma (GB) cells and in a rat preclinical GB model. The CEC extract reduced in vitro cell viability and biomass. In vivo, the extract decreased the tumor volume approximately 62%, without inducing systemic toxicity. The deficit in locomotion and memory and an anxiolytic-like behaviors induced in the GB model were minimized by CEC. The extract decreased the levels of reactive oxygen species, nitrites and thiobarbituric acid reactive substances and increased the activity of antioxidant enzymes in platelets, sera and brains of GB animals. The activity of NTPDases, 5'-nucleotidase and adenosine deaminase (ADA) was evaluated in lymphocytes, platelets and serum. In platelets, ATP and AMP hydrolysis was reduced and hydrolysis of ADP and the activity of ADA were increased in the control, while in CEC-treated animals no alteration in the hydrolysis of ADP was detected. In serum, the reduction in ATP hydrolysis was reversed by CEC. In lymphocytes, the increase in the hydrolysis of ATP, ADP and in the activity of ADA observed in GB model was altered by CEC administration. The observed increase in IL-6 and decrease in IL-10 levels in the serum of GB animals was reversed by CEC. These results demonstrate that CEC extract is a potential complementary treatment to GB, decreasing the tumor size, while modulating aspects of redox and purinergic systems.
RESUMO
Phenolic compounds were extracted from biphasic olive pomace and their biological potential was characterised. Two different extracts were prepared, E1 (40% methanol) and E2 (80% methanol), both subjected to agitation (180 min) and 70 °C. LC-ESI-qTOF-MS was used for individual quantification of the extracted phenolic compounds. The antioxidant activity was determined using different methods, including nitric oxide, DPPH (2,2-diphenyl-1-picrylhydrazyl) and hydroxyl radical. The enzymes α-amylase and α-glucosidase were used to evaluate the antihyperglycemic potential and sulforhodamine B and MTT (tetrazolium salt) for antitumor activity. To evaluate the antimicrobial activity, minimum inhibitory concentration and minimum bactericidal concentration of the extracts, the disc diffusion technique was used. Among the phenolic compounds present, tyrosol was highlighted in both extracts, as well as the anti-hyperglycemic effects, antitumor action and antioxidant activity. The antimicrobial activity found in the extracts was considered moderate to weak.