Exploring ways to improve China's ecological well-being amidst air pollution challenges using mixed methods.

Ecological well-being performance (EWP), a novel concept in sustainable development research, diverges from traditional ecological efficiency in terms of perspectives, core content, and driving factors. However, research on EWP remains insufficiently comprehensive, particularly the corresponding theoretical and methodological investigations into driving pathways. To address this gap, this study develops an "economy-environment-health" framework, incorporating air pollution and associated health losses into the evaluation system, and employs a two-stage Super-NSBM and Window DEA model for reevaluating EWP. The study further investigates the primary pathways of EWP, focusing on environmental regulations, technological innovation, and structural adjustments through both quantitative and qualitative methods. Quantitative spatial econometric analysis reveals that factors such as market-driven environmental regulations, green invention patents, and industrial and energy consumption structures significantly enhance EWP. While examining the "net effects" contributions of individual variables using spatial econometric models, the fsQCA method is employed to identify four effective driving paths for EWP from a configurational perspective. These paths are 1) technological innovation and structural adjustment under environmental regulations with public participation; 2) a combination of environmental regulation, technological innovation, and structural adjustment; 3) structural adjustment with minimal influence from environmental regulations and technological innovation; and 4) structural adjustment directed by market-incentive environmental regulations.

Impact of anti-dumping on global embodied air emissions: a complex network perspective.

After the rise of trade protectionism, anti-dumping has become a common means of political and trade games between countries. Global supply chains move production emissions between countries or regions through trade. In the context of carbon neutrality, anti-dumping measures representing the right to trade may become a tool for the game of emission rights between countries. Therefore, it is very important to study the environmental effects of anti-dumping to cope with global climate change and promote national development. Taking a sample of 189 countries and regions from the EORA input-output table with a study period of 2000-2016, we use the complex network, multi-regional input-output and panel regression models to verify the impact of anti-dumping on air emission transfer by constructing an anti-dumping network and an embodied air emission network. The results show that the initiator of anti-dumping can use anti-dumping to realize the cross-border transfer of ecological costs, reduce the burden of emission reduction and save more on emission quota. Developing countries lacking the right to speak in trade will increase the export volume of commodities after being subjected to a large number of anti-dumping sanctions, thus paying higher ecological costs and consuming more emission quotas. From a global perspective, additional emissions from product production can further contribute to global climate change.

Study on the Drivers of Inclusive Green Growth in China Based on the Digital Economy Represented by the Internet of Things (IoT).

With the vigorous development of digital economy based on digital technologies such as Internet of things (IoT), big data, and artificial intelligence, new vitality has been injected into China's economic model. Inclusive green growth (IGG) supports the transformation of society towards a better quality of life and well-being, as well as environmental protection. Therefore, it is crucial to identify the main drivers of IGG. However, IGG is subject to a variety of interpretations and lacks definitional clarity. To brigade this gap, this study primarily evaluates the performance of IGG and explores the key drivers on IGG in China. Specifically, the data envelopment analysis (DEA) model is employed to calculate IGG for 281 cities in China during 2005-2020. Subsequently, we take advantage of a nest of machine learning (ML) algorithm to demonstrate the vital drivers of urban IGG, which avoids the defects of endogenous linear hypothesis of traditional econometric methods. The results indicate that digitization represented by the IoT and other digital technology is the core drivers of the urban IGG in the overall sample, accounting for about 50% among all of drivers. This finding provides new evidence supporting the "high-quality development" strategy in China, as well as shedding light on grasping the principal fulcrum to achieve the transformation towards IGG in developing economies similar to China.

Effects of lytS-L on the primary metabolism and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona.

The LytTR family two-component system widely exists in bacterial cells and plays an important role in metabolic regulation. The lytS-L gene that encodes for a LytTR family sensor kinase was knocked out to study its influence on the growth, phenotype, and the biosynthesis of the insecticidal polyketide butenyl-spinosyn in Saccharopolyspora pogona NRRL 30141 (S. pogona). High performance liquid chromatography (HPLC) results showed that the butenyl-spinosyn yield of the lytS-L knockout mutant decreased by 58.9% compared with that of the parental strain. This is manifested by a weak toxicity of the mutant against the insect Helicoverpa assulta (H. armigera). Comparative proteomic analysis revealed the expression characteristics of the proteins in S. pogona and S. pogona-ΔlytS-L: a total of 14 proteins involved in energy metabolism were down-regulated, 9 proteins related to carbon metabolism such as glycolysis, and tricarboxylic acid cycle (TCA) were up-regulated, while 13 proteins involved in the biosynthesis of butenyl-spinosyn were down-regulated (fold change >1.2 or< 0.83). The qRT-PCR (Quantitative Real-time PCR) analysis illustrated that the changes in the expression levels of transcription and translation of the identified genes were consistent. This study explores the function of the two-component system of the LytTR family in S. pogona and shows that the lytS-L gene has an important influence on regulating primary metabolism and butenyl-spinosyn biosynthesis of S. pogona.

[Effect of fcl gene for butenyl-spinosyn biosynthesis and growth of Saccharopolyspora pogona].

The fcl gene encodes GDP-fucose synthase, which catalyzes two-step differential isomerase and reductase reactions in the synthesis of GDP-L-fucose from GDP-D-mannose. It also participates in the biosynthesis of amino sugar and ribose sugar, and is one of the key enzymes to regulate the metabolism of sugar and nucleotides in organisms. The presence of fcl gene in Saccharopolyspora pogona was found through sequencing result of genome. The mutant S. pogona-fcl and S. pogona-Δfcl were constructed by gene engineering technology. The results showed that the gene had an effects on growth and development, protein expression and transcriptional level, insecticidal activity, and biosynthesis of butenyl-spinosyn of Saccharopolyspora pogona. The results of HPLC analysis showed that the yield of butenyl-spinosyn in S. pogona-Δfcl was 130% compared with that in S. pogona, which reduced by 25% in S. pogona-fcl. The results of determination of insecticidal activity showed that S. pogona-Δfcl had a stronger insecticidal activity against Helicoverpa armigera than that of S. pogona, while the S. pogona-fcl had a lower insecticidal activity against Helicoverpa armigera compared with S. pogona. Scanning electron microscopy (SEM) was used to observe the morphology of the mycelia. It was found that the surface of the S. pogona-Δfcl was wrinkled, and the mycelium showed a short rod shape. There was no significant difference in mycelial morphology between S. pogona-fcl and S. pogona. Aboved all showed that deletion of fcl gene in S. pogona hindered the growth and development of mycelia, but was beneficial to increase the biosynthesis of butenyl-spinosyn and improve insecticidal activity. Whereas the fcl gene over-expression was not conducive to the biosynthesis of butenyl-spinosyn and reduced their insecticidal activity. SDS-PAGE results showed that the difference of protein expression among the three strains was most obvious at 96 hours, which was identified by real-time fluorescence quantitative polymerase chain reaction, the results showed that there were significant differences of related genes in transcriptional levels among the three strains. Based on the results of the study, a network metabolic control map was constructed to analyze the effect of fcl gene on growth and the regulation pathway of butenyl-spinosyn biosynthesis, which provided an experimental basis for revealing the regulation mechanism of butenyl-spinosyn biosynthesis and related follow-up studies.

Influence of titanium dioxide nanoparticles on the transport and deposition of microplastics in quartz sand.

The influence of titanium dioxide nanoparticles (nTiO2) on the transport and deposition of polystyrene microplastics (MPs) in saturated quartz sand was investigated in NaCl solutions with ionic strengths from 0.1 to 10 mM at two pH conditions (pH 5 and 7). Three different-sized polystyrene (PS) MPs (diameter of 0.2, 1, and 2 µm) were concerned in present study. We found that for all three different-sized MPs in NaCl solutions (0.1, 1 and 10 mM) at both pH 5 and 7, lower breakthrough curves and higher retained profiles of MPs with nTiO2 copresent in suspensions relative to those without nTiO2 were obtained, demonstrating that the copresence of nTiO2 in MPs suspensions decreased MPs transport and increased their deposition in quartz sand under all examined conditions. The mechanisms contributing to the increased MPs deposition with nTiO2 in suspensions at two pH conditions were different. The formation of MPs-nTiO2 heteroaggregates and additional deposition sites provided by previously deposited nTiO2 were found to drive to the increased MPs deposition with nTiO2 in suspensions at pH 5, while the formation of MPs-nTiO2 aggregates, additional deposition sites and increased surface roughness induced by the pre-deposited nTiO2 on quartz sand surfaces were responsible for the enhanced MPs deposition at pH 7. The results give insights to predict the fate and transport of different-sized MPs in porous media in the copresence of engineered nanoparticles.

Constructing Hierarchical Porous Carbons With Interconnected Micro-mesopores for Enhanced CO2 Adsorption.

A high cost-performance carbon dioxide sorbent based on hierarchical porous carbons (HPCs) was easily prepared by carbonization of raw sugar using commercially available nano-CaCO3 as a double-acting template. The effects of the initial composition and carbonization temperature on the micro-mesoporous structure and adsorption performance were examined. Also, the importance of post-activation behavior in the development of micropores and synthesis route for the formation of the interconnected micro-mesoporous structure were investigated. The results revealed excellent carbon dioxide uptake reaching up 2.84 mmol/g (25oC, 1 bar), with micropore surface area of 786 m2/g, micropore volume of 0.320 cm3/g and mesopore volume of 0.233 cm3/g. We found that high carbon dioxide uptake was ascribed to the developed micropores and interconnected micro-mesoporous structure. As an expectation, the optimized HPCs offers a promising new support for the high selective capture of carbon dioxide in the future.

Influence of graphene oxide on the transport and deposition behaviors of colloids in saturated porous media.

The effects of graphene oxide (GO) on the transport and deposition behaviors of colloids with different sizes in packed quartz sand were investigated in both NaCl (10 and 50 mM) and CaCl2 solutions (1 and 5 mM) at pH 6. Fluorescent carboxylate-modified polystyrene latex microspheres (CMLs) with size ranging from 0.2 to 2 µm were utilized as model colloids. Both breakthrough curves and retained profiles of colloids in the presence and absence of GO in suspensions under all examined solution conditions were analyzed. The breakthrough curves of all three different-sized CMLs with GO were higher yet the retained profiles were lower than those without GO at both examined ionic strengths in NaCl solutions. The observation showed that GO increased the transport and decreased the deposition of all three different-sized CMLs in NaCl solutions. However, in CaCl2 solutions, opposite observation was achieved at two different ionic strength conditions. Specifically, the presence of GO increased the transport and decreased the deposition of all three different-sized CMLs in 1 mM CaCl2 solutions, whereas, it decreased the transport and increased the deposition of all three different-sized CMLs in 5 mM CaCl2 solutions. Comparison the breakthrough curves and retained profiles of CMLs versus those of GO yielded that the overall transport and deposition behaviors of all three different-sized CMLs with GO copresent in suspensions agreed well with the transport and deposition behaviors of GO under all examined conditions. The transport and deposition behaviors of CMLs in packed porous media clearly were controlled by those of GO under the conditions investigated in present study due to the adsorption of CMLs onto GO surfaces. Our study showed that once released into natural environment, GO would adsorb (interact with) different types of colloids and thus have significant influence on the fate and transport of colloids in porous media.

Effect of different-sized colloids on the transport and deposition of titanium dioxide nanoparticles in quartz sand.

Colloids (non-biological and biological) with different sizes are ubiquitous in natural environment. The investigations regarding the influence of different-sized colloids on the transport and deposition behaviors of engineered-nanoparticles in porous media yet are still largely lacking. This study investigated the effects of different-sized non-biological and biological colloids on the transport of titanium dioxide nanoparticles (nTiO2) in quartz sand under both electrostatically favorable and unfavorable conditions. Fluorescent carboxylate-modified polystyrene latex microspheres (CML) with sizes of 0.2-2 µm were utilized as model non-biological colloids, while Gram-negative Escherichia coli (∼ 1 µm) and Gram-positive Bacillus subtilis (∼ 2 µm) were employed as model biological colloids. Under the examined solution conditions, both breakthrough curves and retained profiles of nTiO2 with different-sized CML particles/bacteria were similar as those without colloids under favorable conditions, indicating that the copresence of model colloids in suspensions had negligible effects on the transport and deposition of nTiO2 under favorable conditions. In contrast, higher breakthrough curves and lower retained profiles of nTiO2 with CML particles/bacteria relative to those without copresent colloids were observed under unfavorable conditions. Clearly, the copresence of model colloids increased the transport and decreased the deposition of nTiO2 in quartz sand under unfavorable conditions (solution conditions examined in present study). Both competition of deposition sites on quartz sand surfaces and the enhanced stability/dispersion of nTiO2 induced by copresent colloids were found to be responsible for the increased nTiO2 transport with colloids under unfavorable conditions. Moreover, the smallest colloids had the highest coverage on sand surface and most significant dispersion effect on nTiO2, resulting in the greatest nTiO2 transport.

Toll-like receptor 4 is involved in a protective effect of rhein on immunoglobulin A nephropathy.

OBJECTIVES: The objective was to investigate the protective effects of rhein on renal histology change and the effects of rhein on renal tissue toll-like receptor (TLR) 4, TLR9, transforming growth factor-ß1 (TGF-ß1) expression in immunoglobulin A nephropathy (IgAN) rats. MATERIALS AND METHODS: Bovine serum albumin-lipopolysaccharide-carbon tetrachloride 4 method was used to establish IgAN model. Thirty-two male sprague dawley rats were randomly divided into the control group, IgAN model group, rhein-prevented group, and rhein-treated group. 24-h urinary protein (UP), creatinine, urea, alanine aminotransferase (ALT), total protein (TP) contents in the serum of rats were detected with automatic biochemical analyzer and renal pathological changes were observed by the hematoxylin and eosin and periodic acid-Schiff staining. The glomerular deposition of IgA was measured by immunofluorescence staining. Real-time polymerase chain reaction and immunohistochemistry were used to detect renal tissue contents of TLR4, TLR9, TGF-ß1 messenger ribonucleic acid and protein expression. RESULTS: The biochemical parameters results of IgAN model rats showed that the 24-h UP excretion and ALT concentration were much higher, and TP concentration was much lower than those of the control group (P < 0.05). Granule-like or mass-like IgA depositions in the mesangial area, glomerular hypercellularity, hyperplasia of mesangial matrix, and tubulointerstitial fibrosis were found in IgAN group. Rhein-prevented and rhein-treated both improved the biochemical parameters and relieved renal pathological injury. The expressions of renal tissue TLR4, TGF-ß1, but not TLR9 were significantly elevated in IgAN model rats (P < 0.05). Rhein-prevented and rhein-treated both inhibited TLR4 and TGF-ß1 expressions. CONCLUSION: Rhein significantly improved the serum and urine biochemical parameters, and attenuated the glomerular pathological changes and tubulointerstitial fibrosis in IgAN rats. The mechanism may involve inhibition of renal TLR4 and TGF-ß1 secretion.

Bt-transgenic cotton is more sensitive to CeO2 nanoparticles than its parental non-transgenic cotton.

Because genetically modified crops are developing widely in the world while nanoparticles (NPs) are being synthesized and applied in various fields, they will have many opportunities for interactions in the future. The effects of NPs on genetically modified crops therefore require investigation. In the present study, CeO2 NPs were revealed to have toxic effects on root biomass of Bt 29317 at 100 and 500 mg L(-1), but had no toxic effects on Jihe 321. Besides, we also studied the effects of CeO2 NPs on nutrient element uptake in transgenic cotton, and found that contents of most nutrient elements (Fe, Ca, Mg, Zn and Na) in roots of Bt 29317 were affected at lower NP concentrations (100 mg L(-1)) compared with Jihe 321. In addition, ICP-MS analysis revealed that CeO2 NPs were more heavily adsorbed by roots of Bt 29317 than Jihe 321, whereas fewer CeO2 NPs were transported from roots to shoots of Bt 29317 than its non-transgenic counterpart. These data confirm that Bt 29317 is more sensitive to CeO2 NPs than its parental non-transgenic cotton, indicating that nanomaterials are potentially more detrimental to transgenic plants than conventional ones.

Effects of rhein on intestinal epithelial tight junction in IgA nephropathy.

AIM: To investigate the effects of rhein on intestinal epithelial tight junction proteins in rats with IgA nephropathy (IgAN). METHODS: Twenty-eight female Sprague-Dawley rats were randomly divided into four groups (7 per group): Control, IgAN, Rhein-treated, and Rhein-prevented. Bovine serum albumin, lipopolysaccharide and CCl4 were used to establish the rat model of IgA nephropathy. The Rhein-treated group was given rhein from week 7 until the rats were sacrificed. The Rhein-prevented group was given rhein from week 1. Animals were sacrificed at the end of week 10. We observed the changes in the intestinal epithelial tight junctions using transmission electron microscopy, and expression of intestinal epithelial tight junction proteins zona occludens protein (ZO)-1 and occludin by immunofluorescence using laser confocal microscopy. Changes in mRNA and protein expression of ZO-1 and occludin were measured by reverse transcriptase polymerase chain reaction and Western blotting. The ratio of urinary lactulose/mannitol was measured by high performance liquid chromatography (HPLC) for assessing the intestinal permeability. RESULTS: In the control group, the tight junctions lied between epithelial cells on the top of the outer side of the cell membrane, and appeared in dense dotted crystal structures, the neighboring cells were binded tightly with no significant gap, and the tight junction protein ZO-1 and occludin were evenly distributed in the intestinal epithelial cells at the top of the junction. Compared with the control group, in the IgAN group, the structure of the tight junction became obscured and the dotted crystal structures had disappeared; the fluorescence of ZO-1 and occludin was uneven and weaker (5.37 ± 1.27 vs 10.03 ± 1.96, P < 0.01; 4.23 ± 0.85 vs 12.35 ± 4.17, P < 0.01); the mRNA expression of ZO-1 and occludin decreased (0.42 ± 0.19 vs 0.92 ± 0.24, P < 0.01; 0.40 ± 0.15 vs 0.97 ± 0.25, P < 0.01); protein expression of ZO-1 and occludin was decreased (0.85 ± 0.12 vs 1.98 ± 0.43, P < 0.01; 0.72 ± 0.15 vs 1.38 ± 0.31, P < 0.01); and the ratio of urinary lactulose/mannitol increased (3.55 ± 0.68 vs 2.72 ± 0.21, P < 0.01). In the Rhein-prevented and Rhein-treated groups, compared with the IgAN group, the intestinal epithelial tight junctions were repaired; fluorescence of ZO-1 and occludin was stronger (11.16 ± 3.52 and 8.81 ± 2.30 vs 5.37 ± 1.27, P < 0.01; 10.97 ± 3.40 and 9.46 ± 2.40 vs 4.23 ± 0.85, P < 0.01); mRNA of ZO-1 and occludin increased (0.81 ± 0.17 and 0.64 ± 0.16 vs 0.42 ± 0.19, P < 0.01; 0.82 ± 0.22 and 0.76 ± 0.31 vs 0.40 ± 0.15, P < 0.01); protein expression of ZO-1 and occludin was increased (2.07 ± 0.41 and 1.57 ± 0.23 vs 0.85 ± 0.12, P < 0.01; 1.34 ± 0.21 and 1.15 ± 0.17 vs 0.72 ± 0.15, P < 0.01); and the ratio of urinary lactulose/mannitol decreased (2.83 ± 0.43 and 2.87 ± 0.18 vs 3.55 ± 0.68, P < 0.01). CONCLUSION: Rhein can enhance the expression of ZO-1 and occludin, repair damaged tight junctions, and protect the intestinal barrier.