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1.
J Antimicrob Chemother ; 75(12): 3593-3600, 2020 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-32790873

RESUMO

BACKGROUND: Carbapenemase-producing Enterobacterales represent a major therapeutic challenge. MBLs, requiring zinc at their catalytic site, could be inhibited by meso-dimercaptosuccinic acid (DMSA), a heavy metal chelator already widely used for treating lead intoxication. OBJECTIVES: To evaluate the activity of carbapenems alone or combined with DMSA against MBL-producing Escherichia coli in a severe murine peritonitis model. METHODS: Isogenic strains of wild-type E. coli CFT073 producing the MBLs NDM-1, VIM-2 and IMP-1, and the control serine carbapenemases OXA-48 and KPC-3 were constructed. MIC determinations and time-kill assays were performed for imipenem, meropenem and ertapenem alone or in combination with DMSA. Infected mice were treated intraperitoneally for 24 h with imipenem, DMSA or their combination. Bacterial counts in peritoneal fluid and spleen were assessed at 24 h. RESULTS: DMSA in combination with each carbapenem caused a significant decrease in the MICs for all MBL-producing strains, in a concentration-dependent manner, but did not provide benefit against non-MBL strains. In mice infected with the NDM-1-producing strain, the combination of imipenem and DMSA significantly reduced bacterial counts in peritoneal fluid (P = 0.0006) and spleen (P < 0.0001), as compared with imipenem alone, with no benefit against the KPC-3-producing and CFT073 strains. DMSA concentrations in plasma of mice were comparable to those obtained in humans with a standard oral dose. CONCLUSIONS: DMSA restores the activity of carbapenems against MBL-producing strains, and its combination with carbapenems appears to be a promising strategy for the treatment of NDM-producing E. coli infections.


Assuntos
Carbapenêmicos , Peritonite , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Carbapenêmicos/farmacologia , Escherichia coli/genética , Camundongos , Testes de Sensibilidade Microbiana , Peritonite/tratamento farmacológico , Succímero , beta-Lactamases/genética
2.
Eur J Obstet Gynecol Reprod Biol ; 252: 198-205, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32622104

RESUMO

OBJECTIVE: To report complications of Acute Fatty Liver of pregnancy (AFLP), a rare liver disease of pregnancy, and identify prognostic factors for mothers and children. STUDY DESIGN: We conducted a retrospective descriptive study over 18 years in three French maternities. Demographic, clinical, biological data, and outcomes of patients and their infants were reviewed. RESULTS: 142,450 pregnancies from centers were studied. Eighteen patients with AFLP were identified The prevalence of AFLP was estimated as 1/7,914 pregnancies. Prolonged prothrombin time was identified as a risk factor of maternal complications (OR = 0.86, p = 0.0493). Gestational age at delivery was the only risk factor associated with fetal or neonate complications (OR = 0.37, p = 0.0417). One boy died of previously undiagnosed ß-oxidation deficiency at eight months. CONCLUSION: In AFLP, prothrombin time must be carefully monitored to anticipate major maternal complications. Infants born to mothers with ALFP should be screened as early as possible for mitochondrial fatty acid oxidation deficiency.


Assuntos
Fígado Gorduroso , Complicações na Gravidez , Fígado Gorduroso/epidemiologia , Feminino , Humanos , Recém-Nascido , Masculino , Gravidez , Complicações na Gravidez/epidemiologia , Prognóstico , Estudos Retrospectivos
3.
Clin Chim Acta ; 495: 451-456, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31051163

RESUMO

CONTEXT: Cerebrospinal fluid (CSF) biomarkers are valuable tools for the diagnosis of neurological diseases. We aimed to investigate within a retrospective multicentric study the final diagnosis associated with very high CSF Tau levels and to identify patterns of biomarkers that would differentiate them in clinical practice, to help clinical biologists into physicians' counseling. PATIENTS AND METHODS: Within the national multicentric network ePLM, we included 1743 patients from January 1, 2008, to December 31, 2013, with CSF biomarkers assayed by the same Innotest assays (protein Tau, phospho-Tau [pTau], and Aß 1-42). We identified 205 patients with protein Tau concentration higher than 1200 pg/mL and final diagnosis. RESULTS: Among those patients, 105 (51.2%) were suffering from Alzheimer's disease, 37 (18%) from sporadic Creuztfeldt-Jakob disease, and 63 (30.7%) from other neurological diseases including paraneoplastic/ central nervous system tumor, frontotemporal dementia, other diagnoses, amyloid angiopathy, Lewy body dementia, and infections of the central nervous system. Phospho-Tau, Aß1-42 and Aß1-42/pTau values differed significantly between the three groups of patients (p < .001). An Aß1-42/pTau ratio between 4.7 and 9.7 was suggestive of other neurological diseases (threshold in AD: 8.3). CSF 14-3-3 was useful to discriminate Alzheimer's disease from Creuztfeldt-Jakob disease in case of Aß1-42 concentrations <550 pg/mL or pTau>60 pg/mL. CONCLUSION: This work emphasizes the interest of a well-thought-out interpretation of CSF biomarkers in neurological diseases, particularly in the case of high Tau protein concentrations in the CSF.


Assuntos
Laboratórios , Proteínas tau/líquido cefalorraquidiano , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/líquido cefalorraquidiano , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fosfoproteínas/líquido cefalorraquidiano , Adulto Jovem , Proteínas tau/metabolismo
4.
J Intern Med ; 284(1): 78-91, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29498764

RESUMO

BACKGROUND: Acute intermittent porphyria (AIP) is an inherited disorder of haem metabolism characterized by life-threatening acute neurovisceral attacks due to the induction of hepatic δ-aminolevulinic acid synthase 1 (ALAS1) associated with hydroxymethylbilane synthase (HMBS) deficiency. So far, the treatment of choice is hemin which represses ALAS1. The main issue in the medical care of AIP patients is the occurrence of debilitating recurrent attacks. OBJECTIVE: The aim of this study was to determine whether chronic hemin administration contributes to the recurrence of acute attacks. METHODS: A follow-up study was conducted between 1974 and 2015 and included 602 French AIP patients, of whom 46 had recurrent AIP. Moreover, we studied the hepatic transcriptome, serum proteome, liver macrophage polarization and oxidative and inflammatory profiles of Hmbs-/- mice chronically treated by hemin and extended the investigations to five explanted livers from recurrent AIP patients. RESULTS: The introduction of hemin into the pharmacopeia has coincided with a 4.4-fold increase in the prevalence of chronic patients. Moreover, we showed that both in animal model and in human liver, frequent hemin infusions generate a chronic inflammatory hepatic disease which induces HO1 remotely to hemin treatment and maintains a high ALAS1 level responsible for recurrence. CONCLUSION: Altogether, this study has important impacts on AIP care underlying that hemin needs to be restricted to severe neurovisceral crisis and suggests that alternative treatment targeting the liver such as ALAS1 and HO1 inhibitors, and anti-inflammatory therapies should be considered in patients with recurrent AIP.


Assuntos
5-Aminolevulinato Sintetase/sangue , Hidroximetilbilano Sintase/fisiologia , Fígado/fisiopatologia , Porfiria Aguda Intermitente/fisiopatologia , Doença Aguda , Animais , Estudos de Coortes , Estudos Transversais , Feminino , Seguimentos , Heme Oxigenase-1/metabolismo , Hemina/administração & dosagem , Hemina/efeitos adversos , Humanos , Fígado/efeitos dos fármacos , Proteínas de Membrana/metabolismo , Camundongos Endogâmicos C57BL , Estresse Oxidativo/efeitos dos fármacos , Porfiria Aguda Intermitente/diagnóstico , Porfiria Aguda Intermitente/epidemiologia , Porfiria Aguda Intermitente/terapia , Recidiva , Fatores de Risco
5.
Rev Med Interne ; 38(4): 250-255, 2017 Apr.
Artigo em Francês | MEDLINE | ID: mdl-27890382

RESUMO

The role of biomarkers in clinical research was recently highlighted in the new criteria for the diagnosis of Alzheimer's disease. Cerebro-spinal fluid (CSF) biomarkers (total Tau protein, threonine 181 phosphorylated Tau protein and amyloid Aß1-42 peptide) are associated with cerebral neuropathological lesions observed in Alzheimer's disease (neuronal death, neurofibrillary tangle with abnormal Tau deposits and amyloid plaque). Aß1-40 amyloid peptide dosage helps to interpret Aß1-42 results. As suggested in the latest international criteria and the French HAS (Haute Autorité de santé) recommendations, using theses CSF biomarkers should not be systematic but sometimes could be performed to improve confidence about the diagnostic of Alzheimer's disease in young subjects or in complex clinical situations. Future biomarkers actually in development will additionally help in diagnostic process (differential diagnosis) and in prognostic evaluation of neurodegenerative diseases.


Assuntos
Doença de Alzheimer/líquido cefalorraquidiano , Doença de Alzheimer/diagnóstico , Biomarcadores/líquido cefalorraquidiano , Demência/diagnóstico , Peptídeos beta-Amiloides/líquido cefalorraquidiano , Pesquisa Biomédica/métodos , Pesquisa Biomédica/tendências , Demência/líquido cefalorraquidiano , Diagnóstico Diferencial , Humanos , Memória/fisiologia , Padrões de Prática Médica , Proteínas tau/líquido cefalorraquidiano
6.
Rev Med Interne ; 37(3): 173-85, 2016 Mar.
Artigo em Francês | MEDLINE | ID: mdl-26774916

RESUMO

The hereditary porphyrias comprise a group of eight metabolic disorders of the haem biosynthesis pathway characterised by acute neurovisceral symptoms, skin lesions or both. Each porphyria is caused by abnormal function of a separate enzymatic step resulting in a specific accumulation of haem precursors. Seven porphyrias are the consequence of a partial enzyme deficiency while a gain of function mechanism has been recently characterised in a novel porphyria. Acute porphyrias present with severe abdominal pain, nausea, constipation, confusion and seizure, which may be life threatening. Cutaneous porphyrias can be present with either acute painful photosensitivity or skin fragility and blisters. Rare recessive porphyrias usually manifest in early childhood with either severe chronic neurological symptoms or chronic haemolysis and severe cutaneous photosensitivity. Porphyrias are still underdiagnosed, but once they are suspected, and depending on the clinical presentation, a specific and simple front line test allows the diagnosis in all symptomatic patients. Diagnosis is essential to institute as soon as possible a specific treatment. Screening families to identify presymptomatic carriers is crucial to prevent chronic complications and overt disease by counselling on avoiding potential precipitants.


Assuntos
Doenças Genéticas Inatas , Doenças Hematológicas , Heme/metabolismo , Porfirias , Doenças Genéticas Inatas/diagnóstico , Doenças Genéticas Inatas/genética , Doenças Genéticas Inatas/terapia , Doenças Hematológicas/diagnóstico , Doenças Hematológicas/genética , Doenças Hematológicas/metabolismo , Doenças Hematológicas/terapia , Heme/genética , Humanos , Porfiria Aguda Intermitente/diagnóstico , Porfiria Aguda Intermitente/genética , Porfiria Aguda Intermitente/metabolismo , Porfiria Aguda Intermitente/terapia , Porfirias/diagnóstico , Porfirias/epidemiologia , Porfirias/genética , Porfirias/terapia
8.
Med Hypotheses ; 75(6): 600-4, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20801583

RESUMO

Cocaine addiction is a chronic disease marked by relapses, co-morbidities and the importance of psychosocial consequences. The etiology of cocaine addiction is complex and involves three types of factors: environmental factors, factors linked to the specific effects of cocaine and genetic factors. The latter could explain 40-60% of the risk for developing an addiction. Several studies have looked for a link between cocaine addiction and the genes of the dopaminergic system: the genes DRD2, COMT, SLC6A3 (coding for the dopamine transporter DAT) and DBH (coding for the dopamine beta hydroxylase) but unfortunately very few well established results. Pharmacogenetic approach could be an interesting opportunity for the future. The gene DBH has particularly been linked with the psychotic effects caused by cocaine. This so-called cocaine-induced psychosis (CIP) or cocaine-induced paranoia may influence the development of cocaine addiction. Indeed, these psychotic symptoms during cocaine exposure could cause an aversive effect limiting the development of an addiction. Several functional alterations caused by different mutations of the genes involved in dopaminergic transmission (principally-1021C>T of the gene DBH, but also Val158Met of the gene COMT, TaqI A of the gene DRD2 and VNTR 9 repeat of the DAT) could result in a cocaine-induced psychosis prone phenotype. We are hypothesising that the appearance of CIP during the first contact with cocaine is associated with a lower risk of developing cocaine addiction. This protective effect could be associated with the presence of one or more polymorphisms associated with CIP. A pharmacogenetic approach studying combination of polymorphism could isolate a sub-group of patients at risk for CIPs but more favorably protected from developing an addiction. This theory could enable a better understanding of the protective factors against cocaine addiction and offer new therapeutic or preventive targets in vulnerable sub-groups exposed to cocaine.


Assuntos
Transtornos Relacionados ao Uso de Cocaína/etiologia , Transtornos Relacionados ao Uso de Cocaína/fisiopatologia , Dopamina beta-Hidroxilase/genética , Farmacogenética/métodos , Psicoses Induzidas por Substâncias/fisiopatologia , Transtornos Relacionados ao Uso de Cocaína/genética , Humanos , Mutação/genética , Psicoses Induzidas por Substâncias/complicações
9.
Ann Biol Clin (Paris) ; 67(6): 629-39, 2009.
Artigo em Francês | MEDLINE | ID: mdl-19939766

RESUMO

Research of new diagnosis or prognosis biomarkers is a major challenge for the management of patients with complex pathologies like cancer. Clinical proteomics is one of the recent approaches to identify these biomarkers in biological fluids. Over the last five years, many problems related to the variability and the quality control of these analyses have been observed. This was notably related to the different preanalytical status of each sample. A strong need for standardization of the critical preanalytical phases (collection, transport, processing, storage...) has been therefore recognized. With this goal in mind, working groups of the "Institut national du cancer" (INCa) and the "Société française de biologie clinique" (SFBC) proposed here preanalytical proteomics guidelines for the most common biological fluids: plasma, serum, urine and cerebrospinal fluid. To goal is to provide the basis for the harmonization of the procedures in clinical laboratories and biobanks to allow an optimal use of biological collections.


Assuntos
Líquidos Corporais/fisiologia , Técnicas de Laboratório Clínico/normas , Técnicas e Procedimentos Diagnósticos/normas , Guias de Prática Clínica como Assunto , Proteômica/métodos , Análise Química do Sangue/normas , Humanos , Prognóstico , Proteinúria/diagnóstico , Proteômica/normas , Urina/química
10.
Ann Biol Clin (Paris) ; 67(6): 641-9, 2009.
Artigo em Francês | MEDLINE | ID: mdl-19939767

RESUMO

The SFBC Working Group on << Preanalytics and multiplex analyses in proteomics >> is presenting a protocol which will allow harmonization of biospecimen research studies on the impact of different preanalytical variations on peptidic and protein analytes. This protocol is based upon standardization of preanalytical options corresponding to different preanalytical variations and different types of biospecimens (serum, plasma, cedrebrospinal fluid and urine). Application of this protocol will allow, not only harmonization of Biospecimen research, but also elaboration of standard nomenclature of the preanalytical steps.


Assuntos
Peptídeos/análise , Proteínas/análise , Proteômica/métodos , Bancos de Espécimes Biológicos/normas , Análise Química do Sangue/normas , Líquido Cefalorraquidiano , Feminino , Humanos , Masculino , Proteômica/normas , Manejo de Espécimes/normas , Terminologia como Assunto , Urina
11.
J Neurol ; 256(6): 904-9, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19252796

RESUMO

We assessed the prevalence of Wernicke encephalopathy (WE) in all 657 cases suspected of Creutzfeldt-Jakob (CJD) referred from 2001 to 2006 to the French Neuropathology Network of CJD. Clinical, biological and imaging data were reviewed when the diagnosis of WE was made at autopsy. No CJD was found in five cases suspected of sporadic CJD. In these five cases, myoclonus had been observed in four, CSF 14-3-3 protein in two. In 14 other cases, WE was combined with CJD, 13 of which were sporadic. These belonged mainly to the molecular variants of sporadic CJD associated with a long duration of disease. This stresses the necessity of remaining alert to the diagnosis of WE when CJD is suspected.


Assuntos
Encéfalo/patologia , Síndrome de Creutzfeldt-Jakob/epidemiologia , Síndrome de Creutzfeldt-Jakob/patologia , Encefalopatia de Wernicke/epidemiologia , Encefalopatia de Wernicke/patologia , Proteínas 14-3-3/líquido cefalorraquidiano , Adulto , Idoso , Idoso de 80 Anos ou mais , Síndrome de Creutzfeldt-Jakob/diagnóstico , Diagnóstico Diferencial , Humanos , Pessoa de Meia-Idade , Mioclonia/epidemiologia , Mioclonia/patologia , Prevalência , Sistema de Registros , Fatores de Tempo , Encefalopatia de Wernicke/diagnóstico , Adulto Jovem
12.
Ann Pharm Fr ; 65(6): 371-81, 2007 Nov.
Artigo em Francês | MEDLINE | ID: mdl-18079669

RESUMO

Molecular pharmacogenetic units have recently been established in several hospital laboratories in France. The clinical impact of these units is still limited and numerous problems of organizational, ethical, legal, technical, social and economical nature remain to be resolved. However, an increasing number of these units, a rise in their activities and an enlargement of their scope of application are foreseeable in the future. Ultimately, these units would significantly contribute to limit the public health problem caused by interindividual variabilities in drug effects. In view of these prospects, it seems essential that such hospital activity should be quickly recognised by the authorities and the various health sectors in France. It is also essential that the problems that arise from such pharmacogenetic activities should be considered by the authorities and would profit from the organization of a national network and from financial guarantees.


Assuntos
Laboratórios Hospitalares/tendências , Farmacogenética/tendências , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos , França , Humanos , Laboratórios Hospitalares/ética , Laboratórios Hospitalares/estatística & dados numéricos , Metiltransferases/deficiência , Metiltransferases/genética , Farmacogenética/ética , Farmacogenética/estatística & dados numéricos , Saúde Pública
13.
Ann Biol Clin (Paris) ; 65(5): 463-71, 2007.
Artigo em Francês | MEDLINE | ID: mdl-17913666

RESUMO

This review focuses on "clinical proteomics" which represents an emerging discipline in biomedical research. "Clinical proteomics" relies on the analysis of the proteome, i.e. the entire set of peptides and proteins present in a biological sample, to provide relevant data for diagnosis, prognosis or therapeutic strategies of human pathologies. This new type of approach has tremendous potential for the diagnosis of complex pathologies or for the early detection of cancers. This article reports the conclusions of a workgroup of the French Society for Clinical Biology (SFBC) 2004-2006 which evaluated the status, the impact and the future development of proteomics in the clinical field. It provides therefore a broad view going from the methods already present in the clinical laboratories (multiplex technologies...), to the tools for clinical and basis research including bioinformatics.


Assuntos
Proteômica/tendências , Biomarcadores/análise , Eletroforese em Gel Bidimensional , Previsões , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Técnicas Analíticas Microfluídicas , Análise Serial de Proteínas , Proteômica/instrumentação , Proteômica/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
15.
J Pharmacol Exp Ther ; 317(2): 724-31, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16461587

RESUMO

A correlation between high plasma serotonin levels and total pulmonary resistance was reported in more than 80% of pulmonary hypertensive patients. When submitted to chronic hypoxia (10% O(2) for more than 3 weeks), wild-type mice develop lung vascular remodeling and pulmonary hypertension. We previously reported that, in contrast, the development of these hypoxia-dependent alterations is totally abolished in mice with permanent (genetic) or transient (pharmacologic) inactivation of the serotonin 5-hydroxytryptamine (5-HT)(2B) receptor. In the present study, we asked whether 5-HT(2B) receptors could be involved in the control of plasma serotonin levels. Further investigating the chronic hypoxic mouse model of pulmonary hypertension, we first show that in wild-type mice, plasma serotonin levels and 5-HT(2B) receptors expression were significantly increased after chronic exposure to hypoxia. This increase appeared before significant changes in remodeling factors could be detected and persisted when the pathology was established. Conversely, in mice with either genetically or pharmacologically inactive 5-HT(2B) receptors, plasma serotonin levels were not modified by chronic hypoxia. We then confirmed that 5-HT(2B) receptors can control plasma serotonin levels by providing in vivo evidence that an acute agonist stimulation of 5-HT(2B) receptor triggers a transient increase in plasma serotonin that is serotonin transporter dependent and blocked by 5-HT(2B) receptor-selective antagonist or genetic ablation. Our data support the notion that a 5-HT(2B) receptor-dependent regulation of serotonin uptake is implicated in the control of plasma serotonin levels.


Assuntos
Hipertensão Pulmonar/sangue , Hipóxia/sangue , Receptor 5-HT2B de Serotonina/fisiologia , Serotonina/sangue , Animais , Feminino , Hipertensão Pulmonar/etiologia , Hipóxia/complicações , Pulmão/irrigação sanguínea , Pulmão/metabolismo , Masculino , Camundongos , Camundongos Mutantes , Artéria Pulmonar/metabolismo , Receptor 5-HT2B de Serotonina/genética , Antagonistas do Receptor 5-HT2 de Serotonina
16.
Biol Reprod ; 74(5): 816-23, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16421231

RESUMO

The first prion-like protein doppel, officially designed as prion protein dublet, does not seem to be needed for prion disease progression, whereas its physiological function seems to be related to male fertility. Its expression is primarily detected in the male genital tract, and Prnd-inactivated male mice are sterile. We investigated the location of Doppel in the testis of various species of mammal to determine its physiological function. Doppel is expressed early during ontogenesis, and is found in both germ cells and Sertoli cells in mice, rats, boars, and humans. Doppel is permanently expressed in the Sertoli cells but at different levels according to species. Its expression in testicular germ cells was primarily detected in spermatids, with a transient presence in the acrosome. These data suggest that Doppel may play a physiological role in acrosome biogenesis and may be of use in studies of patients suffering from idiopathic infertility.


Assuntos
Acrossomo/metabolismo , Príons/metabolismo , Espermátides/metabolismo , Testículo/metabolismo , Acrossomo/ultraestrutura , Animais , Anticorpos , Proteínas Ligadas por GPI , Humanos , Imuno-Histoquímica , Masculino , Camundongos , Microscopia Eletrônica de Transmissão , Ratos , Ratos Wistar , Espermátides/crescimento & desenvolvimento , Espermátides/ultraestrutura , Suínos , Testículo/crescimento & desenvolvimento
17.
Neurology ; 64(8): 1455-7, 2005 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-15851745

RESUMO

Inherited prion diseases are characterized by mutations in the PRNP gene encoding the prion protein (PrP). We report a novel missense mutation in the PRNP gene (resulting in a G114V mutation in PrP) in members of a Uruguayan family with clinical and histopathologic features of prion disease. Affected individuals were characterized by an early age at onset, initial neuropsychiatric symptoms, late dementia with prominent pyramidal and extrapyramidal symptoms, and long disease duration.


Assuntos
Amiloide/genética , Encéfalo/fisiopatologia , Predisposição Genética para Doença/genética , Mutação/genética , Doenças Priônicas/genética , Precursores de Proteínas/genética , Adolescente , Adulto , Idade de Início , Substituição de Aminoácidos/genética , Biópsia , Encéfalo/metabolismo , Encéfalo/patologia , Aberrações Cromossômicas , Análise Mutacional de DNA , Demência/genética , Demência/patologia , Demência/fisiopatologia , Progressão da Doença , Evolução Fatal , Feminino , Lobo Frontal/metabolismo , Lobo Frontal/patologia , Lobo Frontal/fisiopatologia , Testes Genéticos , Humanos , Masculino , Transtornos da Personalidade/genética , Transtornos da Personalidade/patologia , Transtornos da Personalidade/fisiopatologia , Doenças Priônicas/patologia , Doenças Priônicas/fisiopatologia , Proteínas Priônicas , Príons , Tratos Piramidais/metabolismo , Tratos Piramidais/patologia , Tratos Piramidais/fisiopatologia , Uruguai
18.
Ann Biol Clin (Paris) ; 63(2): 121-6, 2005.
Artigo em Francês | MEDLINE | ID: mdl-15771969

RESUMO

Human prion diseases are rare neurodegenerative diseases, due to proteinaceous infectious particles, named prions. The most frequent of these rare diseases is Creutzfeldt-Jakob disease, which can be sporadic, inherited or acquired (iatrogenic or variant). The diagnosis is based on the post mortem examination of the brain. During the life of the patient, neuronal markers may be detected in CSF, the prion protein gene PRNP may be screened for pathogenic mutations linked to inherited prion disease forms, and the pathogenic prion protein may be evidenced in the tonsils of patients affected with the variant form of the disease. The agent responsible of the disease is still imperfectly known, and the recent discovery of the "prion like" proteins did not help at this point to elucidate the mystery.


Assuntos
Doenças Priônicas , Príons/genética , Animais , Ataxia Cerebelar/diagnóstico , Ataxia Cerebelar/genética , Códon , Síndrome de Creutzfeldt-Jakob/diagnóstico , Síndrome de Creutzfeldt-Jakob/genética , Diagnóstico Diferencial , Modelos Animais de Doenças , Eletroencefalografia , Ensaio de Imunoadsorção Enzimática , Heterozigoto , Homozigoto , Humanos , Doença Iatrogênica , Complexo Principal de Histocompatibilidade , Camundongos , Mutação , Polimorfismo Genético , Proteínas PrPSc/análise , Proteínas PrPSc/genética , Doenças Priônicas/líquido cefalorraquidiano , Doenças Priônicas/diagnóstico , Doenças Priônicas/genética , Príons/análise , Proteínas tau/líquido cefalorraquidiano
20.
FEBS Lett ; 536(1-3): 61-5, 2003 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-12586339

RESUMO

Doppel protein has been discovered in prnp knock-out mouse lines, with overproduction of this protein in the brain causing ataxia and neurodegeneration. We investigated whether Doppel expression (i) affected or was affected by the course of prion propagation in neuroblastoma cells, or (ii) modulated Creutzfeldt-Jakob disease pathogenesis. No change in Doppel production was detected in N2a cells, before or after infection. Transient murine Doppel gene expression had no effect on N2a viability or PrP(Sc) production. A sensitive immunometric assay revealed low levels of Doppel in human brain, reflecting weak transcription of the corresponding gene. No difference in brain Doppel levels was observed between Creutzfeldt-Jakob disease patients and controls, adding further evidence that Doppel is unlikely to be involved in prion disease pathogenesis.


Assuntos
Encéfalo/metabolismo , Síndrome de Creutzfeldt-Jakob/metabolismo , Príons/metabolismo , Animais , Síndrome de Creutzfeldt-Jakob/genética , Feminino , Proteínas Ligadas por GPI , Humanos , Masculino , Camundongos , Neurônios/metabolismo , Príons/genética , RNA Mensageiro/biossíntese , Scrapie/metabolismo , Transcrição Gênica , Células Tumorais Cultivadas
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