Altrenogest Supplementation during Early Pregnancy Improves Reproductive Outcome in Pigs.

Progesterone plays an important role in initial conceptus development and in a successful pregnancy, but results related to progesterone or its analogues (altrenogest) supplementation in early pregnancy of pigs are conflicting. The present study evaluated the effects of altrenogest supplementation in sows during days 6 and 12 of pregnancy on reproductive performance. On day 6 of pregnancy, 301 females were allocated at random to one of the following treatments: CON (Control: non-supplemented females, n = 163) or ALT (females daily supplemented with 20 mg of altrenogest, orally, from day 6 to 12 of pregnancy, n = 138). Ovulation was considered as occurred at 48 h after the first estrus detection to standardize the first day of pregnancy. The supplementation increased the number of total piglets born (ALT: 17.3 ± 0.4; CON: 16.6 ± 0.4), piglets born alive (ALT: 15.6 ± 0.4; CON: 14.8 ± 0.3), and placenta weight (ALT: 4.2 ± 0.1; CON: 3.8 ± 0.1) and decreased the stillbirth rate (ALT: 5.9 ± 0.6; CON: 7.6 ± 0.6) and the number of piglets born weighing less than 800 g (ALT: 6.6 ± 0.6; CON: 8.0 ± 0.6), without impairment on farrowing rate. These results demonstrated that altrenogest supplementation on swine females between days 6 and 12 of pregnancy may be used to improve reproductive performance.

Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets.

The application of phytases for animal feed in developing countries is limited due to the high cost of these enzymes, determined by the importation fees and the expensive substrates used for their production. In this work, we have used agroindustrial byproducts for the production of extracts containing phytases, which were accessed for their stability focusing on the conditions found in the gastrointestinal tract of pigs. The fungus Acremonim zeae presented higher phytase production in medium containing cornmeal, while the yeast Kluyveromyces marxianus produced 10-fold more phytase when cultivated on rice bran. Process optimization increased the difference in productivity to more than 300 fold. The phytase from A. zeae was thermostable, with higher activity at neutral pH and 50 °C, but was inhibited at pH 2.5 and by various ions. The phytase activity in the K. marxianus extract was stable at a wide range of conditions, which indicates the presence of at least two enzymes. As far as we know, this manuscript describes for the first time the phytase production and the characteristics of the extracts produced by both these microbial species. These enzymes could be produced at low cost and have potential to replace enzymes currently imported for this purpose.